Objective: To screen the antimicrobial activity of Varnya lepa lotion against skin infecting organisms like Staphylococcus aureus and Staphylococcus epidermis. In Ayurvedic literature, Varnya lepa is one of formulation which includes seven drugs like Raktachandan, Manjista, Kustha, Lodhra, Priyangu, Vatankura and Masura. It is mainly indicated for melasma, enhances complexion and hyperpigmentation. Methods: The antimicrobial activity of Varnya lepa lotion was testing by using Agar well diffusion method/ Cup-plate method and Minimum inhibitory concentration(MIC). Results:Through the Disc diffusion method, Varnya lepa lotion has shown no antibacterial activity but by MIC method has minimum antibacterial activity against Staphylococcus aureus and Staphylococcus epidermis organisms. Conclusion: As herbal products are growing in the market tremendously and concerns surrounding their safety are also increasingly recognised. In this article, it is concluded that Varnya lepa lotion was having a minimum antibacterial effect against the organisms like Staphylococcus aureus and Staphylococcus epidermis and would protect skin from different infections.