On 25 June 2020, a glacial lake outburst flood (GLOF) occurred in Jinwuco, Nidou Zangbo, and southeast Tibet, causing catastrophic damage to multiple infrastructures such as roads, bridges, and farmlands in the surrounding and downstream areas. Due to the lack of long-term monitoring of glacial lake and glacier changes in the region and the surrounding surface, the spatial and temporal evolutionary characteristics and triggering factors of the disaster still need to be determined. Here, we combine multi-temporal optical remote sensing image interpretation, surface deformation monitoring with synthetic aperture radar (SAR)/InSAR, meteorological observation data, and corresponding soil moisture change information to systematically analyze the spatial and temporal evolution characteristics and triggering factors of this GLOF disaster. Optical images taken between 1987 and 2020 indicate that the glacial lake’s initial area of 0.39 km2 quickly grew to 0.56 km2, then plummeted to 0.26 km2 after the catastrophe. Meanwhile, we found obvious signs of slippage beside the lateral moraine at the junction of the glacier’s terminus and the glacial lake. The pixel offset tracking (POT) results based on SAR images acquired before and after the disaster reveal that the western lateral moraine underwent a 40 m line of sight (LOS) deformation. The small baseline subset InSAR (SBAS-InSAR) results from 2017 to 2021 show that the cumulative deformation of the slope around the lateral moraine increased in the rainy season before the disaster, with a maximum cumulative deformation of −52 mm in 120 days and gradually stabilized after the disaster. However, there are three long-term deformation areas on the slope above it, showing an increasing trend after the disaster, with cumulative deformation exceeding −30 mm during the monitoring period. The lateral moraine collapse occurred in a warm climate with continuous and intense precipitation, and the low backscatter intensity prior to the slide suggests that the soil was very moist. Intense rainfall is thought to be the catalyst for lateral moraine collapse, whereas the lateral moraine falling into the glacier lake is the direct cause of the GLOF. This study shows that the joint active–passive remote sensing technique can accurately obtain the spatial and temporal evolution characteristics and triggering factors of GLOF. It is helpful to understand the GLOF event caused by the slide of lateral moraine more comprehensively, which is essential for further work related to glacial lake hazard assessment.