Amnion Cells In Culture Research Articles

A comparison of the growth of selected mycobacteria in HeLa, monkey kidney, and human amnion cells in tissue culture.

HeLa, monkey kidney, and human amnion cells in tissue cultures were compared as sites for the multiplication of strains of tubercle bacilli or original and reduced pathogenicity, and for several other species of mycobacteria capable of causing disease in humans. The arrangement of the pathogenic species inorder of their growth rates in HeLa cells was Mycobacterium fortuitum, Mycobacterium balnei, and the "yellow bacillus," followed closely by the tubercle bacillus. This order was also correct for these species in monkey kidney and human amnion cells, and is the same as that seen in bacteriological media. The arrangement of the strains of tubercle bacilli in order of their growth rates in all three types of cells was: H37Rv, then R1Rv, and lastly H37Ra, which multiplied about as slowly as BCG. An INH-resistant strain grew about as rapidly as H37Rv. Growth of the pathogenic species occurred at about the same rates in HeLa and monkey kidney cells, but was distinctly slower in human amnion cells, which are less active metabolically. Irradiation of the cells in doses up to 5000 r did not affect the subsequent growth of mycobacteria in them. Preliminary experiments with human leprosy bacilli indicate that they can be introduced into these cells in high numbers and that the bacilli then persist for the life of the cells.

Metabolism of human amnion cell cultures infected with poliomyelitis virus. I. Glucose metabolism during virus synthesis.

Metabolism of human amnion cell cultures infected with poliomyelitis virus. I. Glucose metabolism during virus synthesis.

Fluorescent antibody studies with herpes simplex virus in unfixed preparations of trypsinized tissue cultures.

Summary: The classical strain of herpes simplex virus (Burnet & Lush, 1939) and a recently isolated strain (Melbourne 1/56) were propagated in tissue culture of human amnion and infant-mouse kidney ‘epithelial’ cells. Following the inoculation of 103·25 TCCD50 (tissue culture cytopathogenic dose) of the Melbourne 1/56 herpes simplex virus into amnion cell culture, the titre of virus dropped to 100·50 TCCD50 at 12 hr. and the maximum level of virus (103·50 TCCD50) was detected at 48 hr. after inoculation. The earliest morphological changes were observed in the tube-cultures c. 18 hr. after inoculation of the virus and eosinophilic intranuclear inclusion bodies were observed in the amnion cells c. 24 hr. after virus injection. Cell degeneration was prominent at 36 hr. when most cells were highly retractile and rounded. Cell suspensions, harvested from infected and uninfected cultures of amnion and mouse- kidney epithelium, were treated with immune herpes serum followed by fluorescent rabbit anti-human γ-globulin, and specific peripheral fluorescence was observed in the unfixed preparations of the infected tissue cultures. The limited surface staining was ascribed to the impermeability of the unfixed cytoplasmic membranes to globulins.

Cultivation of measles virus in human amnion cells and in developing chick embryo.

Summary1. A strain of measles virus originally isolated in cultures of human renal cells has been propagated throughout 28 serial passages in cultures of human amnion cells. In the latter system it induces 2 types of cytopathic change: (1) formation of “syncytia” or “multinuclear giant cells” in which intranuclear and intracytoplasmic inclusions are prominent features; (2) only recently recognized, the assumption by individual epithelial cells of a characteristic fusiform or stellate configuration. In certain of these affected cells eosinophilic intranuclear inclusions are present that resemble those found in the nuclei of the syncytia. Eventually both types of change terminate in cellular necrosis and disintegration. In contrast to the dual response of amnion cells only the formation of syncytia has been observed in cultures of renal cells infected with the virus. 2. The Edmonston strain of measles virus from the 28th passage in human amnion cells was inoculated into chick embryos. In this host it has be...

Human amnion cell cultures; susceptibility to viruses and use in primary virus isolations.

Summary1. Tissue cultures of human amnion cells were found to be susceptible to all adenoviruses tested, certain Coxsackie viruses, and herpes simplex virus. 2. Amnion cell cultures were employed in large scale routine testing for viral agents with recovery of 40 strains of adenoviruses types 1, 2, 3, and 5, one type 1 poliovirus, and 6 strains of herpes simplex virus.

Plaque Formation of Poliomyelitis Viruses on Human Amnion Cell Cultures.

SummaryPlaque formation of all 3 types of poliomyelitis virus has been obtained in monolayer cultures of human aminon cells. The plaque titer obtained on aminon was 3-6 times higher than on monkey kidney cell cultures for strains Mahoney and MEF-1.