Plant Culture Research Articles

Challenges Experienced by Aquaponic Hobbyists, Producers, and Educators

We used an online survey to document challenges experienced by aquaponic hobbyists (n = 81), producers (n = 117), and educators (n = 75). Responses were distilled into the following categories: 1) operations and management; 2) facilities, location, and system design; 3) knowledge and educational resources; 4) funding; 5) economic viability; 6) plant culture; 7) marketing and distribution; 8) fish culture; 9) human factors; 10) regulations and certifications. Training and research in these areas are needed to advance the aquaponics industry.

Optimisation of substrate composition and level of mineral nutrition as the basis of improving the production of decorative plants in container culture

The current significant increase in the volume of growing ornamental seedlings in container culture in tree nurseries is conditioned by a number of significant advantages of planting material with an uninjured root system. At the same time, the agricultural technology for the production of such seedlings in container culture is much more complicated, compared to the traditional one. First of all, this is conditioned by growing them in a space limited by the size of containers and using an artificially prepared substrate. The purpose of the study was to conduct biotesting of three modifications of the substrate composition for the container culture of Spiraea japonica ‘Goldflame’, Tamarix tetrandra Pall. ex Bieb., Forsythia ovate Nakai and different doses of starter fertilisers “Nitroamofoska” and “Plantacote”. The study used both general scientific methods of analysis, synthesis, and active experimentation, as well as applied research methods such as biometric, phenological, soil, and agrochemical. Studies have established the presence of species-specific reactions of experimental plants with different fastidiousness to soil conditions, to the composition of the substrate and the types and doses of starter fertiliser used in container culture. A conclusion was made regarding the increase in the profitability of their cultivation due to the use of cheaper local components for the preparation of the substrate. The conducted studies showed a number of advantages of using organo-mineral slow-release fertilisers in container culture as a starter, in particular, “Plantacote” at a dose of 2.5-5.0 g∙l-1. That the mass production of decorative planting material with a closed root system should be preceded by research on establishing species-specific reactions of cultivated plants to cultivation conditions, which are the basis for optimising the composition of the substrate and the level of mineral nutrition of cultivated plants in order to increase the efficiency of the production of decorative seedlings in container culture. The obtained results will be useful for producers of planting material and the scientific community working towards the development of container culture of ornamental plants

‘Op de knalmaat van de bylen’. Geweld en verzet in de Surinaamse plantagepoëzie van P.F. Roos

How are colonial violence and resistance entangled in Dutch plantation poetry? This article aims to answer this question through an analysis of Paul François Roos’Surinaamsche mengelpoëzy (1804), a collection of poems describing Suriname’s plantation culture. Previously, its representations of Suriname’s natural environment have been interpreted as exotic variations on pastoral conventions, offering a Eurocentric perspective on the colony. This article hypothesises that, even if literary conventions in the poems contributed to the concealment of colonial violence against the natural environment and the Afro-American population, an affective analysis can also reveal voices of dissent. To do so, it will combine ecocritical and postcolonial theory to demonstrate how Surinaamsche mengelpoëzy ‘naturalised’ distinctions between ‘nature’ and ‘culture’, and between ‘harmony’ and ‘combat’, asserting colonial order. However, when this representation of Suriname’s plantation culture is conceptualised as an ‘affective economy’ in which human and non-human bodies are interrelated through economic and ecological affects, these distinctions start to blur, and order is destabilised. Using Monique Allewaert’s concept of ‘ecological personhood’ ecological affects become visible as Afro-American resistance against European notions of individuality and sovereignty.

Effect of planting density, foliar spraying and overlapping system on the growth and productivity using soilless culture system

The aim of this study to improve the production of tomato and strawberry plants in soilless culture. This experiment was carried out to study effect of the planting distances (D30, D40), Overlapping (mixed culture) of the tomato plant only and with strawberry plant (L0, L1), and foliar application by Biozyme (B0, B1) on growth and productivity of the plants. The results revealed that planting of tomato at a distance 40 cm by using ring culture (soilless) had a significant superiority in the chlorophyll content (61.15); diameter of the tomato stem (13.47 mm); fruits number of tomato (22.78); fruit weight of tomato (206.88 g) and total plant yield of tomato (4.71 kg.plant−1). In addition, for strawberry plant had a significant superiority in the fruits number (16.25); fruit weight (25.20 g), total plant yield (408.55 g plant−1, 1634 g m−2 and 389.47 kg greenhouse-1). The planting of tomato at a distance 30 cm had a significant superiority in the plant high (210.88 cm) and total plant yield of tomato (22.86 kg m−2 and 5.69 ton greenhouse-1). Foliar application of Biozyme alone and combined treatment D30 B1 had a significant superiority in the yield of the strawberry plant (340.08 g plant−1 and 269.70 g.plant−1, respectively).

The effect of auxin status driven by bacterivorous nematodes on root growth of Arabidopsis thaliana

Bacterivorous nematodes are one of the dominant microbial organisms and play an important role in shaping the bacterial community and hormone dynamics in rhizosphere soils. It has been found that the presence of bacterivorous nematodes potentially regulate indole-3-acetic acid (IAA) content in soils by interacting with the status of IAA-producing bacteria, and eventually act on the growth of plant roots. However, the mechanism behind this interaction in broad soil ecosystems remains unknown. This study reveals the interaction between bacterivorous nematodes and the soil bacterial community, especially IAA-producing bacteria, based on denaturing gradient gel electrophoresis (DGGE) analysis. The results of DGGE showed that the bacterial diversity in soil was increased by inoculation of nematodes during certain periods of plant culture, and was also affected by the genotype of the Arabidopsis thaliana (Johannes Thal, 1577) plants. The variation in soil bacterial communities among the treatments with different genotypes of A. thaliana and nematodes indicated that the soil bacterial community structure was affected by both genotype and nematode grazing. Furthermore, we measured the root architectures of four genotypes of A. thaliana, including wild type (Col-0), two IAA-insensitive mutants (Axr5 and Axr3–1), and DR5::GUS transgenic type (IAA sensitive) under different conditions of bacterivorous nematode inoculation. We found that the root architecture of the IAA-sensitive A. thaliana developed more tips and slender roots under inoculation with activated bacterivorous nematodes, while the root architectures of auxin-insensitive A. thaliana mutants showed no significant differences between alive and inactivated bacterivorous nematodes, even though the IAA content increased in the soil under inoculation with alive nematodes. This study confirms that the presence of bacterivorous nematodes in soils increases the soil IAA content significantly by regulating the bacterial community structure, which leads to an accumulation of auxin in the root tips of A. thaliana, and eventually enhances the plant root growth.

Synthesis, Biosynthesis, and Biological Activity of Diels-Alder Adducts from Morus Genus: An Update.

The plants of the Moraceae family are producers of a great variety of polyphenolic natural products. Among these, the Diels-Alder type adducts (DAAs) are endowed with a unique cyclohexene scaffold, since they are biosynthesized from [4+2] cycloaddition of different polyphenolic precursors such as chalcones and dehydroprenyl polyphenols. To date, more than 150 DAAs have been isolated and characterized from Moraceous and related plants. The main source of DAAs is the mulberry root bark, also known as "Sang-Bai-Pi" in Traditional Chinese Medicine, but they have also been isolated from root bark, stem barks, roots, stems or twigs, leaves, and callus cultures of Moraceous and other related plants. Since 1980, many biological activities of DAAs have been identified, including anti-HIV, antimicrobial, anti-inflammatory, and anticancer ones. For these reasons, natural DAAs have been intensively investigated, and a lot of efforts have been made to study their biosynthesis and to establish practical synthetic access. In this review, we summarized all the updated knowledge on biosynthesis, chemoenzymatic synthesis, racemic and enantioselective total synthesis, and biological activity of natural DAAs from Moraceous and related plants.

Effects of plasma and its activated water on gene expression of Medicago truncatula

AbstractIn this study, the seeds of Medicago truncatula are treated with plasma and plasma‐activated water (PAW), and the RNA sequencing (RNA‐Seq) of the seedlings is performed to analyze the gene expression changes. It is found that plasma treatment has no significant effect on seed survival rate, but PAW has an obvious lethal effect. Both methods result in the differential expression of a large number of genes in seedlings, and the expression of genes with the function of promoting plant growth and metabolism is significantly upregulated. The roots of seedlings in PAW group grow lateral roots. This study provides new ideas and experimental evidence for PAW plant culture research and has a guiding significance for leguminous plant breeding.

Tea Environments and Plantation Culture: Imperial Disarray in Eastern India

Tea Environments and Plantation Culture: Imperial Disarray in Eastern India

An Investigation into Using Temporary Immersion Bioreactors to Micropropagate Moringa oleifera Lam. Callus, Roots, and Shoots

Moringa oleifera Lam., a tree naturally grown in the tropics, is becoming increasingly popular as an industrial crop due to its multitude of useful attributes. Therefore, this study tested the effect of temporary immersion system (TIS) bioreactors for mass micropropagation of Moringa oleifera Lam. callus, roots, and shoots. TIS are tissue culture systems that make use of timers to periodically immerse and drain plant cultures in a liquid nutrient medium instead of using solidified media. In initial studies, Moringa oleifera seeds were germinated in vitro, and in vitro seedling leaflets were then used as explant material for callus production on the pre-culturing media. Two experiments were conducted to improve the protocol for TIS bioreactor production. The first experiment investigated the effect of 6-benzylaminopurine (BA) and kinetin, whereas experiment 2 was conducted to improve shooting production. For the first experiment, leaf material was cultured onto a solidified medium consisting of half-strength Murashige and Skoog (MS) basal salts and 0.5 ppm 1-naphthaleneacetic acid (NAA) to initiate callus production before splitting it between solidified media and bioreactors for shooting. The shooting media consisted of full-strength MS basal salts and different treatments of kinetin and BA. A significant increase in callus production was observed with the use of TIS bioreactors, compared to solidified media, whereas root production had a highly significant interaction effect between the media and the cytokinin treatments. With shoot proliferation in mind, experiment 2 was performed, where microcuttings from in vitro-grown seedlings were excised and cultured onto a solidified MS medium, consisting of a control (0 ppm BA) and two different concentrations of 6-benzylaminopurine (BA) (0.1 ppm BA and 0.2 ppm BA) in the pre-culturing phase. Microcuttings were again excised after two weeks and transferred to the shooting media containing 0.1 ppm BA in TIS bioreactors and semi-solidified medium. Results showed TIS bioreactors to be effective in increasing both the amount and length of shoots produced. Shoot and callus fresh weights were also higher in explants cultured in TIS bioreactors. The results of this study also suggest M. oleifera sensitivities to plant growth regulators (PGRs). In conclusion, this study successfully produced callus, roots, and shoots in both the solidified media and TIS bioreactors, emphasizing the prospect of using TIS bioreactors for mass micropropagation of M. oleifera callus, roots, and shoots.

Plant growth acceleration using a transparent Eu3+-painted UV-to-red conversion film

The stimulation of photosynthesis is a strategy for achieving sustainable plant production. Red light is useful for plant growth because it is absorbed by chlorophyll pigments, which initiate natural photosynthetic processes. Ultraviolet (UV)-to-red wavelength-converting materials are promising candidates for eco-friendly plant cultures that do not require electric power. In this study, transparent films equipped with a UV-to-red wavelength-converting luminophore, the Eu3+ complex, were prepared on commercially available plastic films for plant growth experiments. The present Eu3+-based films absorb UV light and exhibit strong red luminescence under sunlight. Eu3+-painted films provide significant growth acceleration with size increment and biomass production for vegetal crops and trees in a northern region. The plants cultured with Eu3+-painted films had a 1.2-fold height and 1.4-fold total body biomass than those cultures without the Eu3+ luminophores. The present film can promote the plant production in fields of agriculture and forestry.

Analysis of phosphorus forms on different soil types using heterogeneous isotope exchange reaction

Pot experiments and soil incubation were carried out to follow the processes of added phosphorus (P) fertilizer using radioisotope tracer technique, heterogeneous isotope exchange. Four types of soils were investigated (Dystric Arenosol (Humic), Calcic Vertisol (Gleyic), Rendzic Phaeozem (Hyperhumic), Calcic Gleysol(Arenic, Humic)). The changes of phosphate fractions (water-soluble (Pw), isotopically exchangeable (PIE), ammonium lactate soluble phosphorus (PAL), and tightly sorbed phosphorus (Ptightly)) in soil were determined as a function of P supply, incubation time and the plant culture. The soil samples were incubated at different times (1, 3, 13 weeks) and phosphate quantities. 0, 40, 80, 160, 320 mg P/kg soil doses of P fertilizer were added to the soils. After incubation, perennial ryegrass (Lolium perenne L.) was sown. A significant correlation was found between the sum of Pw and PIE and Puptake of plant proving that these values can be good indicators of plant available phosphorus. During plant culture, the Pw and PIE values always decrease showing that plant directly utilizes these phosphate forms. The transformation of added phosphate to tightly sorbed phosphate is the highest for soils with great humus content.

Extracts of Fruits and Plants Cultivated In Vitro of Aristotelia chilensis (Mol.) Stuntz Show Inhibitory Activity of Aldose Reductase and Pancreatic Alpha-Amylase Enzymes.

Aristotelia chilensis is a plant whose fruit is considered a powerful natural antioxidant. During the last years, some investigations of the fruit have been carried out, finding antioxidant properties in the juice or the phenolic fraction. The antioxidant properties of the plant are useful in the inhibition of enzymes related to diabetes such as pancreatic aldose reductase and alpha-amylase. Because many synthetic drugs used today have limitations and potentially harmful side effects, the use of naturally occurring compounds, such as flavonoids, is clinically attractive. In this study, the characterization of aqueous extracts of fruits and in vitro plants of A. chilensis was carried out based on their content of anthocyanins and total phenols, the antioxidant capacity by the antiradical activity 2,2-diphenyl-1-picrilhydrazil (DPPH), and the profile of anthocyanins and other phenolic compounds by liquid chromatography coupled to mass spectrometry (LC-MS/MS). Subsequently, the effect of these extracts on the inhibition of bovine aldose reductase and pancreatic alpha-amylase enzymes was determined. According to our results, extracts of fruits and in vitro plants of A. chilensis achieved inhibition of the bovine aldose reductase enzyme of 85.54 ± 1.86% and 75.67 ± 1.21%, respectively. Likewise, the percentage of inhibition of the pancreatic alpha-amylase enzyme for fruit extracts was 29.64 ± 0.63%, while for in vitro plant extracts it was 47.66 ± 0.66%. The antioxidant and enzymatic inhibition activity of the extracts were related to the content of anthocyanins, such as delphinidin and cyanidin glycosides as well as the phenols derived from quercetin, myricetin, and kaempferol. The results obtained allow us to suggest that the in vitro culture of plants of A. chilensis represents a viable biotechnological alternative to obtain phenolic compounds for the inhibition of aldose reductase and pancreatic alpha-amylase enzymes.

Enhancing control systems of higher plant culture chambers via multilevel structural mechanistic modelling.

Modelling higher plant growth is of strategic interest for modern agriculture as well as for the development of bioregenerative life support systems for space applications, where crop growth is expected to play an essential role. The capability of constraint-based metabolic models to cope the diel dynamics of plants growth is integrated into a multilevel modelling approach including mass and energy transfer and enzyme kinetics. Lactuca sativa is used as an exemplary crop to validate, with experimental data, the approach presented as well as to design a novel model-based predictive control strategy embedding metabolic information. The proposed modelling strategy predicts with high accuracy the dynamics of gas exchange and the distribution of fluxes in the metabolic network whereas the control architecture presented can be useful to manage higher plants chambers and open new ways of merging metabolome and control algorithms.

In Vitro Propagation of Pyracantha angustifolia (Franch.) C.K. Schneid.

Narrow-leaf firethorn or pyracantha (Pyracantha angustifolia (Franch.) C.K. Schneid.), from the family Rosaceae, is a species of large and thorny evergreen shrub. In this study, a procedure is presented for efficient axillary shoot multiplication and root induction in P. angustifolia using Murashige and Skoog (MS), woody plant (WPM), and Linsmaier and Skoog (LS) culture media supplanted with 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA). The disinfection of the axillary buds was performed with a 70.23% success rate on a basal MS medium augmented with 0.5 mg·L−1 gibberellic acid (GA3). Uniform and axenic explants were then cultured on MS, WPM, and LS media enriched with different concentrations of BAP, 0.3 mg·L−1 GA3, and 0.1 mg·L−1 IBA. The highest multiplication coefficient (2.389) was obtained for the MS medium supplemented with 2.5 mg·L−1 BAP. After one month, newly formed micro-shoots were transferred to rooting media (MS, WPM, and LS) containing different concentrations of IBA, together with a constant concentration of 0.1 mg·L−1 BAP. The micro-shoots were kept in the dark for one week and then cultured in a 16/8 h light/dark regime. The MS medium supplemented with 1 mg·L−1 IBA was the most effective in stimulating rooting (88.76% of micro-shoots). The highest number of roots (3.5 per micro-shoot) was produced in the MS medium enriched with 1.5 mg·L−1 IBA. The rooted plantlets were transferred into pots filled with perlite and peat moss in a 2:1 proportion and acclimatized to ambient greenhouse conditions, with a resultant mean 92.84% survival rate. Thus, this protocol can be successfully applied for the in vitro mass propagation of P. angustifolia.

Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

BackgroundFlavonoids are necessary for plant growth and resistance to adversity and stress. They are also an essential nutrient for human diet and health. Among the metabolites produced in Cannabis sativa (C. sativa), phytocannabinoids have undergone extensive research on their structures, biosynthesis, and biological activities. Besides the phytocannabinoids, C. sativa is also rich in terpenes, alkaloids, and flavonoids, although little research has been conducted in this area.ResultsIn this study, we identified 11 classes of key enzyme-encoding genes, including 56 members involved in the flavonoid biosynthesis in C. sativa, from their physical characteristics to their expression patterns. We screened the potentially step-by-step enzymes catalyzing the precursor phenylalanine to the end flavonoids using a conjoin analysis of gene expression with metabolomics from different tissues and chemovars. Flavonol synthase (FLS), belonging to the 2-oxoglutarate-dependent dioxygenase (2-ODD) superfamily, catalyzes the dihydroflavonols to flavonols. In vitro recombinant protein activity analysis revealed that CsFLS2 and CsFLS3 had a dual function in converting naringenin (Nar) to dihydrokaempferol (DHK), as well as dihydroflavonols to flavonols with different substrate preferences. Meanwhile, we found that CsFLS2 produced apigenin (Api) in addition to DHK and kaempferol when Nar was used as the substrate, indicating that CsFLS2 has an evolutionary relationship with Cannabis flavone synthase I.ConclusionsOur study identified key enzyme-encoding genes involved in the biosynthesis of flavonoids in C. sativa and highlighted the key CsFLS genes that generate flavonols and their diversified functions in C. sativa flavonoid production. This study paves the way for reconstructing the entire pathway for C. sativa’s flavonols and cannflavins production in heterologous systems or plant culture, and provides a theoretical foundation for discovering new cannabis-specific flavonoids.

Polish Contribution to Global Research on Somatic Embryogenesis

AbstractSomatic embryogenesis (SE) is a complex process that begins with regaining totipotency in some somatic cells, proceeds through embryo development and maturation, and ends with the formation of a whole plant. Since the first publications on SE in 1958, this regeneration process has been applied to the in vitro propagation of many plant species and has led to the development of some specific model systems. SE has been used to expand our understanding of the cytomorphological, physiological, biochemical, and genetic processes that govern the earliest developmental events in the life of plants. This paper summarizes the achievements of Polish research groups working on SE systems established for several plants (gentians, the tree fernCyathea delgadiiSternb., and conifers) and three model species (Arabidopsis thaliana,Medicagosativa, andM. truncatula). SE systems have used a broad spectrum of experimental approaches involving genomic tools (transcriptomics, proteomics, and chromatin analyses), physiological methods which focus on phytohormones, and cytological techniques. Studies on the experimental models ofA. thalianaandMedicagospp. have resulted in the identification of new genetic and epigenetic elements of the complex regulatory network controlling embryogenic induction in plant somatic cells. The protocol developed for ferns has provided a unique and simple system for cytological analysis of early SE events that occur in a single cell of initial explants. Gentian embryogenic suspension cultures have successfully been used in broad biotechnological applications, including plant transformation, protoplast isolation, culture, and fusion. Systems described for coniferous species effectively produced many vigorous somatic seedlings and cost-efficient storage of genotypes during clonal field-testing. The research undertaken by Polish scientists has resulted in developing experimental systems that have enabled significant advances in SE knowledge.

Endogenous phthalates as a prospective regulator of interspecific relations in a biocoenosis

It is widely believed that phthalates are xenobiotic pollutants whose prevalence in the environment is associated with their facilitated diffusion from plastic materials. Studies into the effect of synthetic phthalates on living organisms revealed their extremely negative action on the metabolism of animals and humans. The acting mechanism of these compounds is realised through a ligand-receptor pathway. Along with dioxins, polychlorinated biphenyls and similar compounds, phthalates are classified as endocrine disrupters. However, at present, sufficient evidence has been accumulated confirming the natural origin of phthalates. Thus, phthalates were de novo biosynthesised from labelled precursors in an algae culture. These compounds were detected in closed experimental systems, including cell cultures of highest plants, as well as those isolated from a number of bacterial, fungi, lowest and highest plant forms located far from the sources of technogenic pollution. The concept of phthalate biogenesis assumes the action of these compounds on living systems. Phthalates exhibit bactericidal and fungicidal action and compose allelopathic exudates, suppressing the growth of competing plant forms. Phthalates possess insecticidal and repellent properties. An analogy can be traced between the action of phthalates and endocrine disrupters of another chemical category, namely phytoestrogens, which regulate herbivorous mammal populations. A hypothesis is proposed about the biological role of endogenous plant phthalates representing secondary metabolic compounds. Exhibiting predominantly a shielding function, these compounds participate in the network of interactions between plants, animals, fungi and microorganisms. It should be noted that synthetic and endogenous phthalates are characterised by essential stereochemical differences, which can explain their different action on living organisms.

Использование и особенности культивирования мха сфагнума в биотехнологической системе для естественной фильтрации, очистки воздуха в городских условиях

This article presents the results of a study of a literature review on the use of mosses as natural filters for air purification in polluted locations; provides information on the features of the cultivation of sphagnum moss by vertical and horizontal methods in the future design of a biotechnological system; describes the necessary conditions to achieve the maximum effect of air purification using this type of plant culture. The study results will be used in the development of unique equipment, namely a pilot prototype of a biotechnological system — a filter using sphagnum moss as a natural filter for air purification in a certain area. This development involves the use of automated control systems to create artificial conditions for plant culture — moss, which, for natural reasons, cannot exist in urban environments. In the long term, a biotechnological filter will effectively clean the air, cool the environment and reduce the noise level in those locations where it is impossible to plant many trees due to large buildings. Currently, groups of scientists around the world are conducting research and experimental development of various solutions, including technologies that can significantly improve the urban environment using natural filters. Using mosses as a natural filter will absorb fine dust, purify the air, and create a favorable space.

RhizoPot platform: A high-throughput in situ root phenotyping platform with integrated hardware and software.

Quantitative analysis of root development is becoming a preferred option in assessing the function of hidden underground roots, especially in studying resistance to abiotic stresses. It can be enhanced by acquiring non-destructive phenotypic information on roots, such as rhizotrons. However, it is challenging to develop high-throughput phenotyping equipment for acquiring and analyzing in situ root images of root development. In this study, the RhizoPot platform, a high-throughput in situ root phenotyping platform integrating plant culture, automatic in situ root image acquisition, and image segmentation, was proposed for quantitative analysis of root development. Plants (1-5) were grown in each RhizoPot, and the growth time depended on the type of plant and the experimental requirements. For example, the growth time of cotton was about 110 days. The imaging control software (RhizoAuto) could automatically and non-destructively image the roots of RhizoPot-cultured plants based on the set time and resolution (50-4800 dpi) and obtain high-resolution (>1200 dpi) images in batches. The improved DeepLabv3+ tool was used for batch processing of root images. The roots were automatically segmented and extracted from the background for analysis of information on radical features using conventional root software (WinRhizo and RhizoVision Explorer). Root morphology, root growth rate, and lifespan analysis were conducted using in situ root images and segmented images. The platform illustrated the dynamic response characteristics of root phenotypes in cotton. In conclusion, the RhizoPot platform has the characteristics of low cost, high-efficiency, and high-throughput, and thus it can effectively monitor the development of plant roots and realize the quantitative analysis of root phenotypes in situ.

Effect of Silver Nanoparticles Synthesized by ‘Green’ Methods on the Growth of in vitro Culture of Betula pendula L. whole Plants

Background: Metal nanoparticles, such as silver nanoparticles obtained by “green” nanosynthesis, have been increasingly used in research and practice in recent years due to their high biocompatibility and low toxicity. It is important to understand how green nanoparticles have regulatory effects on all groups of living systems, including plants. One of the key questions is how silver nanoparticles obtained by green methods modify plant growth in various cultivation and biotechnological systems, such as in vitro culture. Objective: The aim of this study was to establish how in vitro culture of birch plants (Betula pendula Roth) reacts to different levels of silver nanoparticles synthesized by green methods (based on plant extracts) and chemical approaches. Methods: The paper examined the nodal segments of silver birch Betula pendula Roth grown on Woody Plant Medium (WPM) with the addition of silver nanoparticles (0.3-300 mg L-1). After 30 days of cultivation in an in vitro environment, the growth of shoots and roots was measured. Silver nanoparticles were synthesized using L-ascorbic acid (reducing agent) and polyvinylpyrrolidone (PVP; stabilizer), as well as with needle extract (as a reducing agent and stabilizer). Results: Chemical nanosynthesis based on PVP and L-ascorbate, as well as green nanosynthesis using extract of spruce needles made it possible to obtain spherical nanoparticles with similar physical parameters. Low levels of AgNPs (0.3-10 mg L-1) synthesized by chemical techniques (PVP and L-ascorbate) stimulated the growth of birch shoots. In this case, the maximum stimulating effect on shoot growth was found at 10 mg of L-1 AgNPs (250-300% stimulation compared to the control). Under higher levels of nanoparticles (30-300 mg L-1), the stimulating effect decreased. Concentrations over 300 mg of L-1 inhibited the growth of birch plants. Very similar effects were observed in roots. In experiments with nanoparticles synthesized using spruce needle extract, it was shown that low concentrations of AgNPs (0.3 and 1 mg L-1) did not cause a significant change in the size of birch shoots and roots. At the same time, higher levels of silver nanoparticles (3-300 mg L-1) significantly stimulated growth. Conclusion: The present study demonstrates the production of stable silver nanoparticles based on PVP and L-ascorbic acid, as well as an extract of Betula pendula needles. The resulting nanoparticles have a uniform shape and distribution. The presence of AgNP (1-300 mg L-1) in the nutrient media has a stimulating effect on Betula pendula shoot and root growth.