CorrigendaCorrigendumPublished Online:01 Jan 2011https://doi.org/10.1152/ajpcell.zh0-6486-corr.2011Original articleMoreSectionsPDF (577 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInEmailWeChat Volume 295, December 2008Volume 64, December 2008Suh HN, Huong HT, Song CH, Lee JH, Han HJ. Linoleic acid stimulates gluconeogenesis via Ca2+/PLC, cPLA2, and PPAR pathways through GPR40 in primary cultured chicken hepatocytes. Am J Physiol Cell Physiol 295: , 2008. First published October 8, 2008; doi:10.1152/ajpcell.00368.2008 (http://ajpcell.physiology.org/cgi/content/full/295/6/C1518).— In the final published version of this article, in results, the blot for β-actin from Fig. 4A was mistakenly also inserted as being representative of cPLA2 and lamin in Figs. 3A and 5B, respectively. This problem has been corrected in the revised Figs. 3A and 5B. The corrected figures are shown below. The authors apologize for the errors. The problems identified did not alter the conclusions reached in this study.Fig. 3.Involvement of cytosolic phospholipase A2 (cPLA2) in linoleic acid-induced gluconeogenesis. A: primary cultured chicken hepatocytes were treated with linoleic acid for 0–240 min and then harvested. The total protein was extracted and blotted with phospho-cPLA2 or cPLA2 antibodies. Values represent means ± SE of three experiments for each condition as determined from densitometry relative to cPLA2. *P < 0.05 vs. control. B: cells were treated with a mixture of EGTA/BAPTA-AM for 30 min before treatment with linoleic acid for 1 h. The total protein was extracted and blotted with the phospho-cPLA2 or cPLA2 antibodies. Values represent means ± SE of three experiments for each condition as determined from densitometry relative to cPLA2. *P < 0.05 vs. control, **P < 0.05 vs. linoleic acid. C: cells were treated with AACOCF3 or mepacrine for 30 min before treatment with linoleic acid for 12 h; [3H]arachidonic acid ([3H]AA) release was then measured. Values represent means ± SE of three independent experiments with triplicate dishes. *P < 0.05 vs. control, **P < 0.05 vs. linoleic acid. D: cells were treated with AACOCF3 or mepacrine for 30 min before treatment with linoleic acid for 12 h; glucose production was then measured. Values represent means ± SE of three independent experiments with triplicate dishes. *P < 0.05 vs. control, **P < 0.05 vs. linoleic acid.Download figureDownload PowerPointFig. 5.Involvement of peroxisome proliferator-activated receptors (PPARs) in linoleic acid-induced gluconeogenesis. A: primary cultured chicken hepatocytes were treated with linoleic acid for 6 h, and the PPAR-α (a), -δ (b), and -γ (c) gene expression levels were then analyzed by real-time RT-PCR. *P < 0.05. B: cells were incubated with linoleic acid for 0–12 h and then harvested. The nuclear fraction was extracted and blotted with PPAR-α, PPAR-δ, or lamin antibodies. Values represent means ± SE of four experiments for each condition as determined from densitometry relative to lamin. *P < 0.05 vs. control, #P < 0.05 vs. control. C: cells were treated with AACOCF3, mepacrine, or indomethacin heptyl ester for 30 min before treatment with linoleic acid for 12 h. The nuclear fraction was extracted and blotted with PPAR-α, PPAR-δ, or lamin antibodies. Values represent means ± SE of four experiments for each condition as determined from densitometry relative to lamin. *P < 0.05 vs. control, **P < 0.05 vs. linoleic acid; #P < 0.05 vs. control, ##P < 0.05 vs. linoleic acid.Download figureDownload PowerPointThis article has no references to display. Download PDF Previous Back to Top Next FiguresReferencesRelatedInformationRelated articlesLinoleic acid stimulates gluconeogenesis via Ca2+/PLC, cPLA2, and PPAR pathways through GPR40 in primary cultured chicken hepatocytes 01 Dec 2008American Journal of Physiology-Cell Physiology More from this issue > Volume 300Issue 1January 2011Pages C222-C223 Copyright & PermissionsCopyright © 2011 the American Physiological Societyhttps://doi.org/10.1152/ajpcell.zh0-6486-corr.2011History Published online 1 January 2011 Published in print 1 January 2011 Metrics