Culture Conditions For Mycelial Growth Research Articles

Culture conditions for mycelial growth of eight Philippine wild strains of tiger sawgill mushroom, Lentinus tigrinus

Lentinus tigrinus is a wild edible and medicinal mushroom with potential culinary and pharmaceutical applications. In the present work, we evaluated the different culture media, pH and physical conditions such as aeration, illumination and temperature for maximum mycelial growth of eight L. tigrinus strains. Mycelial growth of the eight strains respond differently to the nutritional and physical factors. All strains showed maximum mycelial growth on coconut water gulaman (CWG), except TLLt 3, which favored potato dextrose agar (PDA) and malt extract agar (MEA). TLLt 1, 5, 6, 7 and 8 mycelia luxuriantly grew at pH 5.0-8.0 whereas TLLt 2 preferred pH 5.0-7.0, and TLLt 3 and 4 favored pH 5.0-6.5. Sealed condition was found to be more suitable for TLLt 1 mycelia while no significant difference was noted between the two aeration conditions for the mycelial growth of other seven strains. Both TLLt 1 and 2 mycelia favorably grew in lighted condition while the other six favorably grew in both lighted and dark conditions. Room temperature (30⁰C) recorded the fastest mycelial growth fo all strains, but found comparable to air-conditioned temperature (23⁰C) for the growth of TLLt 3, 6 and 8 mycelia. Therefore, mycelial growth of L. tigrinus on the different nutritional and physical parameters may vary depending on the strain type.

Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces

Termitomyces sp. that grow in symbiosis with fungus-farming Termites have medicinal properties. However, they are rare in nature, and their artificial culture is challenging. The expression of AXL receptor tyrosine kinase and immune checkpoint molecules favor the growth of cancer cells. The study evaluated the optimal conditions for the artificial culture of Termitomyces and their inhibitory activity on AXL and immune checkpoint molecules in lung adenocarcinoma and melanoma cell lines. The culture of 45 strains of Termitomyces was compared. Five strains with marked growth rates were selected. Four of the selected strains form a single cluster by sequence analysis. The mycelium of 4 selected strains produces more fungal mass in potato dextrose broth than in a mixed media. The bark was the most appropriate solid substrate for Termitomyces mycelia culture. The mycelium of all five selected strains showed a higher growth rate under normal CO2 conditions. The culture broth, methanol, and ethyl acetate of one selected strain (T-120) inhibited the mRNA relative expression of AXL receptor tyrosine kinase and immune checkpoint molecules in cancer cell lines. Overall, these results suggest the potential usefulness of Termitomyces extracts as a co-adjuvant therapy in malignant diseases.

Optimization of mycelial growth and cultivation of wild Ganoderma sinense.

Ganoderma sinense, a well-known medicinal macrofungus of Basidiomycetes, is widely used in traditional medicine for promoting health and longevity in East Asia. The fruiting bodies of G. sinense contain polysaccharides, ergosterol, and coumarin, which have antitumor, antioxidant, and anticytopenia activities. Mushroom cultivation requires suitable conditions for the formation of fruiting bodies and yield. However, little is known about the optimal culture conditions for mycelial growth and cultivation of G. sinense. In this study, the successful cultivation of a G. sinense strain collected from the wild was reported. The optimal culture conditions were identified by examining one factor at a time. The results of this study revealed that the nutritional requirements for the optimal mycelial growth of G. sinense were fructose (15 g/l) as the carbon source and yeast extract (1 g/l) as the nitrogen source. The optimal pH and temperature for G. sinense were 7 and 25-30°C, respectively. The mycelia grew fastest in treatment II (69% rice grains + 30% sawdust + 1% calcium carbonate). G. sinense produced fruiting bodies under all tested conditions and showed the highest biological efficiency (2.95%) in treatment B (96% sawdust, 1% wheat bran, 1% lime). In summary, under optimal culture conditions, G. sinense strain GA21 showed satisfactory yield and a high potential for commercial cultivation.

Optimization of mycelial growth of <i>Xylaria papulis</i> Lloyd (Xylariaceae) in indigenous liquid culture conditions, science city of Muñoz, Nueva Ecija, Philippines

The search for potential and inexhaustible sources of bioactive compounds with great functional activities is imperative for potential drug leads in treating various human diseases. In this regard, this study investigated the optimum liquid medium and physical culture conditions for the mycelial growth of <italic>X. papulis</italic> Lloyd for nutraceutical studies. The greatest mycelial biomass was achieved in PSB liquid medium among the indigenous media evaluated, such as potato sucrose broth (PSB), corn meal broth (CMB), rice bran broth (RBB), and coconut water (CW). In terms of pH, <italic>X. papulis</italic> Lloyd was observed to grow in a wide range of pH (5.0−8.5), but the best mycelial growth was observed at pH 6.5. Room temperature of 28 °C, lighted (137 lux), and static conditions were the other optimum physical culture conditions for mycelial growth of <italic>X. papulis</italic> Lloyd.

Optimization of culture conditions for mycelial growth and fruiting body production of naturally-occurring Philippine mushroom Lentinus swartzii Berk

Lentinus swartzii, belonging to Basidiomycota, is one of the unrecorded and un-utilized naturally-occurring mushrooms in the Philippines. With the intention to realize the full potential of this wild mushroom, the present work highlights the successful rescue, domestication, optimization of culture conditions (culture media, pH, and physical factors) for mycelial growth, and evaluation of the fruiting body production on formulated substrate. The mycelia favorably grew on coconut water gulaman (CWG), potato sucrose gulaman (PSG), and potato dextrose agar. However, for practical and economic reasons, CWG was the basal medium used in the evaluation of pH and physical factors. The better-quality mycelial growth was obtained when cultured on CWG at pH 6, and incubated in the dark, 30°C, under sealed conditions. Both rice seeds and cracked corn were the most suitable materials for grain spawn production. Among substrate formulations, 7:3 ratio of rice straw and sawdust produced the highest mean weight of fruiting bodies (37.02 g per bag) and biological efficiency (7.40%). Enriched cultivation of this mushroom by adding nutritious supplements in the substrate must be done in further studies.

Optimization of Culture Conditions for Mycelial Growth and Sporulation of Myrothecium roridum

Culture and nutrition conditions of Myrothecium roridum Tode were optimized by conducting a series of interlined experiments on a growth medium, temperature, pH, and photoperiod. In contrast, relation of culture age with virulence was measured by fungal development on young leaves of bitter gourd. The physiological response was measured on colony radial growth and spore production. Among the six test growth media, i.e., nutrient agar (NA), potato dextrose agar (PDA), Czapek-Dox agar (CDA), glucose agar (GA), malt extract agar (MEA), and bitter gourd agar (BGA), the highest radial growth (77 mm) and the highest number of spores (239 × 106 spores/ml) were observed on PDA. Incubation temperature was evaluated between a range of 15-40 °C, and the highest colony growth (87 mm) was observed at 30 °C, whereas the highest spore production (315 × 106 spores/ml) was at 35 °C. Different pH levels, i.e., 5, 5.5, 6, 6.5, 7, and 7.5, were optimized, and the highest colony growth (87 mm) and spore production (504 × 106 spores/ml) was recorded at pH 5.0. Impact of photoperiod was studied, and the highest mycelial growth (88 mm) and maximum spore production (524 × 106 spores/ml) was observed at 16/8 h alternate light and dark period. It was concluded that the optimum conditions for mycelia growth and spore production was pH 5.0-6.0 and at 30 ± 2 °C in PDA with 16/8 h alternate light and dark photoperiod.

DETERMINATION OF OPTIMUM CULTURE CONDITIONS FOR MYCELIAL GROWTH OF Macrolepiota procera MUSHROOM

Macrolepiota procera, commonly called the Parasol Mushroom, is a delicious mushroom collected from the nature and commonly consumed by the public in many regions of Turkey. This study was conducted to determine the optimum culture conditions (pH, temperature, carbon and nitrogen sources) for mycelial growth of M. procera. Three pH values (pH 5.0, 5.5 and 6.0), four incubation temperatures (15, 20, 25 and 30°C), seven carbon (C) sources (dextrose, glucose, lactose, maltose, mannitol, sucrose and xylose) and six nitrogen (N) sources ((NH4)2HPO4, NH4NO3 and Ca(NO3)2, malt extract, peptone and yeast extract) were investigated. In the second step of the study, the effect of seven pH values (4.0, 4.5, 5.0, 5.5, 6.0, 6.5 and 7.0) on the mycelial colony diameter was examined at 20 and 25°C since these temperatures gave the best mycelial growth in the previously conducted temperature experiment. The best mycelial growth was determined at pH 6.0. The optimum temperature for mycelial growth of M. procera was found as 25°C. The use of glucose as carbon source and yeast extract and peptone as nitrogen source in the culture medium gave the best results for mycelial growth. Determining of optimum culture conditions for mycelial growth of M. procera will provide important contributions to the fortcoming studies on it’s commercially cultivation in Turkey.

Optimal culture conditions for mycelial growth and fruiting body formation of Ling Zhimushroom Ganoderma lucidum strain GA3

The objective in this study is to investigate optimal culture conditions for mycelial growth and fruiting body formation of the Ling Zhi mushroom, Ganoderma lucidum strain GA3. The results of the study show that the optimal media and temperature for the mycelial growth are potato, glucose, and agar (PGA) supplemented with rice bran, and 25-300C, respectively. Strain GA3 is able to grow in a wide pH range, between 4 and 12.The most favourable substrate mixture for the formation and development of the fruiting body is 87% sawdust + 4% corn powder + 8% rice bran + 1% calcium carbonate (CaCO3).

Nutritional Requirements for Mycelial Growth of Milk-White Toothed Mushroom, Irpex lacteus (Agaricomycetes), in Submerged Culture.

Irpex lacteus, a medicinal fungus, is used in traditional Chinese medicine to treat chronic glomerulonephritis. In this work, a strain of I. lacteus was isolated from the fruiting body of a wild specimen and identified by ITS-5.8S ribosomal DNA sequencing analysis. Then the nutritional requirements and culture conditions for mycelial growth of I. lacteus in semisynthetic liquid media were investigated using the one-factor-at-a-time and orthogonal matrix methods. Optimum growth occurred at 30°C and 35°C. I. lacteus mycelia grew well at pH values between 3 and 9, suggesting that this strain is not sensitive to pH. The nutritional components, including 9 carbohydrates, 9 nitrogen compounds, 11 vitamins, and 10 mineral elements, were studied for their effects on mycelial growth in submerged cultures of I. lacteus. Among these variables, soluble starch, peptone, yeast extract, and calcium chloride were identified as required for optimum mycelial growth. The concentrations of each component were optimized using an orthogonal design, and the effects of medium composition on mycelial growth were found in the order soluble starch > yeast extract > peptone > calcium chloride. The optimal concentrations of these components for mycelial growth were determined to be 60 g/L soluble starch, 35 g/L peptone, 15 g/L yeast extract, and 0.6 g/L calcium chloride. Under the optimum medium and culture conditions, the maximum biomass reached 13.73 g/L after 3 days in submerged culture, a value over twice that reached using the basal medium. These results provide a basis for further physiological study and industrial fermentation of I. lacteus.

Culture conditions for mycelial growth of Poria cocos

This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and <TEX>$35^{\circ}C$</TEX>, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and <TEX>$MgSO_4{\cdot}7H_2O$</TEX> and <TEX>$FeSO_4{\cdot}7H_2O$</TEX> as mineral salts.

Optimization of culture conditions for mycelial growth and basidiocarp production of Philippine strains of Panaeolus antillarium and Panaeolus cyanescens

The domestication and optimization of cultural conditions for the secondary mycelial growth was determined to establish the production technology of Panaeolus species. Wild P. antillarum and P. cyanescens were collected and their cell lines were rescued. The mycelial growth performance was evaluated on different indigenous culture media (carabao, cow and horse dung) and physical factors (pH, aeration and illumination). The optimal growth condition for basidiocarp production using dung of domesticated ruminants was also determined. Both secondary mycelia of the two Panaeolus species evaluated grew best on solid culture media of carabao and cow dung decoction gulaman (local crude agar) at a pH range of 7.5 - 8.0, incubated in unsealed and dark condition. Carabao and cow dung as substrates favored the fast mycelial colonization of both mushrooms with means of 11.20 ± 4.24 and 11.50 ± 5.80 days, respectively. However, regardless of the substrate, P. antillarum colonized the substrates in a shorter period of 9.37 ± 2.74 days. In terms of yield, carabao dung had the highest mean yield of 7.16 ± 0.75 g with 17.89 ± 1.88% bio-efficiency, which did not significantly vary with cow dung having a mean yield of 6.99 ± 1.89 g with 17.49 ± 4.73% bio-efficiency. These significant results suggest that P. antillarum and P. cyanescens are new Philippine strains of wild mushrooms with great potential for cultivation for nutraceutical purposes.

The culture conditions for mycelial growth and sclerotial formation of Polyporus umbellatus

Polyporus umbellatus (Syn. Grifola umbellata) is a sclerotium forming mushroom belongs to family Polyporaceae of Polyphorales, Basidiomycota. The sclerotia of P. umbellatus have long been used for traditional medicines in China, Korea and Japan. This study was initiated to obtain the basic data for artificial sclerotial production of P. umbellatus. Here, we investigated the favorable conditions for mycelial growth of P. umbellatus and its symbiotic fungus Armillaria mellea. We also evaluate the favorable carbon and nitrogen sources for sclerotial formation in dual culture between P. umbellatus and A. mellea. The favorable conditions for mycelial growth of P. umbellatus were 20oC and pH 4, while optimal conditions for mycelial growth of A. mellea were 25oC and pH 6. The carbon sources for optimal mycelial growth of P. umbellatus were fructose and glucose, while carbon sources for favorable mycelial growth of A. mellea were also fructose and glucose. The nitrogen sources for favorable mycelial growth P. umbellatus were peptone and yeast extract, while optimal mycelial growth of A. mellea were obtained in peptone and yeast extract. When P. umbellatus and A. mellea were dual cultured on carbon sources, sclerotia were induced on basal media supplemented with glucose, fructose and maltose at pH 4~6, while nitrogen sources inducing sclerotia were basal media supplemented with peptone and yeast extract for 60 days at 20oC under dark condition.

Optimization of Submerged Culture Conditions for Mycelial Growth and Extracellular Polysaccharide Production by Coriolus versiolor

This paper is concerned with optimization of submerged culture conditions for mycelial growth and Exopolysaccharides (EPS) yield with Coriolus versiolor by both one-factor-at-a-time and orthogonal matrix methods. Glucose and yeast-Extracts were identified to be the most suitable carbon and nitrogen sources, respectively. The optimal initial pH, inoculum size and liquid volume for mycelial growth and EPS yield were 5.0, 8% and 150mL/500mL, respectively. Subsequently, the concentration of glucose, yeast-extract, KH2PO4, and MgSO4•7H2O were optimized using the orthogonal matrix method. The effects of media composition on the mycelial growth of Coriolus versiolor were in the order of glucose >KH2PO4 > yeast-extract > MgSO4•7H2O, and those on EPS yield were in the order of glucose >MgSO4•7H2O> yeast-extract >KH2PO4.The optimal concentrations for enhanced yield were determined as 30 g/L glucose, 7.0 g/L yeast-extract, 1.0 g/L KH2PO4, 1.0 g/L MgSO4•7H2O, and 40 g/L glucose, 6.0 g/L yeast-extract, 1.0 g/L KH2PO4, 1.5 g/L MgSO4•7H2O for mycelial and EPS yield, respectively. The verification experiments confirmed the final medium. This optimization strategy (30 g/L glucose, 7.0 g/L yeast-extract, 2.0 g/L KH2PO4 and 0.5 g/L MgSO4•7H2O) in shake flask culture lead to a mycelial yield of 5.18 g/L, and EPS yield of 0.64 g/L, respectively, which were considerably higher than those obtained in preliminary studies. Under optimal culture conditions, the maximum EPS concentration in a 5-L stirred-tank bioreactor was 0.75 g/L, while the maximum mycelial yield was 8.55 g/L. This also corresponded to 14.67% and 39.42% enhancement in EPS yield and mycelial dry weight, respectively, compared with the verification test results.

Favorable Culture Conditions for Mycelial Growth of Korean Wild Strains in Ganoderma lucidum

Ganoderma lucidum (Fr.) Karst (Polyporaceae), belonging to basidiomycota, is one of the most famous medicinal mushrooms. This study was carried out to investigate favorable mycelial growth conditions, such as pH, temperature, growth media, carbon sources and nitrogen sources of Korean strains in G. lucidum. The most suitable temperature for the mycelial growth was obtained at 30°C. In general, optimal temperature range for the mycelial growth was found at 25~30°C. This Mushroom has a broad pH range (5~9) for its mycelial growth and mostly favorable growth was found at pH 5. Generally, Hamada, Glucose peptone, YM, Mushroom complete and Lilly media were the most suitable for the mycelial growth of G. lucidum. Among 10 different carbon sources, dextrin, galactose and fructose were best but the rest of other carbon sources also facilitated the growth of mycelia. The most suitable nitrogen sources were ammonium acetate, glycine, arginine and calcium nitrate, but to a certain extent, all of the supplemented nitrogen sources also stimulated the mycelial growth.

Production of exopolysaccharides by submerged mycelial culture of a mushroom Tremella fuciformis

The optimization of submerged culture conditions for mycelial growth and exopolysaccharide (EPS) production in an edible mushroom Tremella fuciformis was studied in shake flasks and bioreactors. The temperature of 28 degrees C and pH 8 in the beginning of fermentation in agitated flasks was the most efficient condition to obtain maximum mycelial biomass and EPS. The optimal medium constituents were as follows (gL(-1)): glucose 20, tryptone 2, KH(2)PO(4) 0.46, K(2)HPO(4) 1 and MgSO(4).7H(2)O 0.5. The fungus was cultivated under various agitation and aeration conditions in a 5L stirred-tank bioreactor. The maximum cell mass and EPS production were obtained at a relatively high agitation speed of 200 rpm and at an aeration rate of 2 vvm. The flow behavior of the fermentation broth was Newtonian and the maximum apparent viscosity (35 cP) was observed at a highly aerated condition (2 vvm). The EPS productivity in an airlift reactor was higher than that in the stirred-tank reactor. The morphological study revealed that the fungus grows in mainly three different yeast-like forms: ovoid, elongated, and double yeast forms. The high population of the elongated yeast has a very close relationship to high EPS production. The EPS were protein-bound polysaccharides consisted of mainly mannose, xylose, and fucose. The molecular weights of EPS were determined to be (1.3-1.5)x10(6).

Optimization of submerged culture conditions for mycelial growth and exo-biopolymer production by Paecilomyces tenuipes C240

This paper is concerned with optimization of submerged culture conditions for mycelial growth and exo-biopolymer production by Paecilomyces tenuipes C240 by one-factor-at-a-time and orthogonal matrix methods. The one-factor-at-a-time method was adopted to investigate the effects of medium components (i.e. carbon, nitrogen, and mineral sources) and environmental factors (i.e. initial pH and temperature) on mycelial growth and exo-biopolymer production. Among these variables, glucose, KNO 3, K 2HPO 4, and MgSO 4 were identified to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature and initial pH for mycelial growth and exo-biopolymer production were 28 °C and 6.0, respectively. Subsequently, the concentration of glucose, KNO 3, K 2HPO 4, and MgSO 4 were optimized using the orthogonal matrix method. The effects of media composition on the mycelial growth of P. tenuipes C240 were in the order of glucose>K 2HPO 4>KNO 3>MgSO 4, and those on exo-biopolymer production were in the order of glucose>K 2HPO 4>MgSO 4>KNO 3. The optimal concentration for enhanced production were determined as 4 g/l glucose, 0.6 g/l KNO 3, 0.1 g/l K 2HPO 4, and 0.1 g/l MgSO 4 · 5H 2O for mycelial yield, and 3 g/l glucose, 0.4 g/l KNO 3, 0.1 g/l K 2HPO 4, and 0.1 g/l MgSO 4 · 5H 2O for exo-biopolymer production, respectively. The subsequent verification experiments confirmed the validity of the models. This optimization strategy in shake flask culture lead to a mycelial yield of 10.18 g/l, and exo-biopolymer production of 1.89 g/l, respectively, which were considerably higher than those obtained in preliminary studies. Under optimal culture conditions, the maximum exo-biopolymer concentration in a 5 l stirred-tank bioreactor was 2.36 g/l.

Optimum Culture Conditions for Aflatoxin B2Production by a Human Pathogenic Strain ofAspergillus Flavus

The optimum culture conditions for mycelial growth and aflatoxin production by a human pathogenic strain of Aspergillus flavus were established. On the basis of its physical and chemical properties...