Sesame Cultivars Research Articles

Sesame production constraints, variety traits preference in the Southeastern Tanzania: Implication for genetic improvement

Despite the prominence of sesame production in household and national economies, its productivity remains quite low (405.6 kg/ha) in comparison to the potential output of 1500 kg/ha. Low sesame production threatens the employment of 481,115 smallholder producers in Tanzania's sesame sub-sector. In view of that, to enhance sesame productivity, the present study lured to find out what restrictions upset sesame cultivation and spot farmers' desired traits for sesame cultivars in southeastern Tanzania. A semi-structured questionnaire and interview checklist were adopted to obtain data from 240 sesame cultivators. The findings revealed that the key impediments inhibiting sesame production in the studied regions included the prevalence of insect-pests (23.6%), diseases (15.7%), drought (10.1%), weed infestation (9.9%), insufficient capital (7.9%), high seed costs (7.6%), and a dearth of enhanced seeds (6.8%). Furthermore, according to sesame growers, the most important criteria in sesame variety selection were high yielding (45.7%), resistance to insect-pests and diseases (20.8%), early maturity (15.4%), appealing seed color (8.8%), and drought tolerance (6.3%). Meanwhile, it was reported that the market valued sesame cultivars with white grain colour, large grain size, clean seeds, and high oil content. Hence, the present study recommends enhanced breeding for improved sesame variety development with the desired traits by farmers and post-farm operators.

Stability analysis for seed yield in ten cultivars of sesame (Sesamum indicum L.)

Stability analysis for seed yield in ten cultivars of sesame (Sesamum indicum L.)

Lignan-Rich Sesame (Sesamum indicum L.) Cultivar Exhibits In Vitro Anti-Cholinesterase Activity, Anti-Neurotoxicity in Amyloid-β Induced SH-SY5Y Cells, and Produces an In Vivo Nootropic Effect in Scopolamine-Induced Memory Impaired Mice.

Alzheimer's disease, a major cause of dementia, is characterized by impaired cholinergic function, increased oxidative stress, and amyloid cascade induction. Sesame lignans have attracted considerable attention owing to their beneficial effects on brain health. This study investigated the neuroprotective potential of lignan-rich sesame cultivars. Among the 10 sesame varieties studied, Milyang 74 (M74) extracts exhibited the highest total lignan content (17.71 mg/g) and in vitro acetylcholinesterase (AChE) inhibitory activity (66.17%, 0.4 mg/mL). M74 extracts were the most effective in improving cell viability and inhibiting reactive oxygen species (ROS) and malondialdehyde (MDA) generation in amyloid-β25-35 fragment-treated SH-SY5Y cells. Thus, M74 was used to evaluate the nootropic effects of sesame extracts and oil on scopolamine (2 mg/kg)-induced memory impairment in mice compared to the control cultivar (Goenback). Pretreatment with the M74 extract (250 and 500 mg/kg) and oil (1 and 2 mL/kg) effectively improved memory disorder in mice (demonstrated by the passive avoidance test), inhibited AChE, and enhanced acetylcholine (Ach) levels. Moreover, immunohistochemistry and Western blot results showed that the M74 extract and oil reversed the scopolamine-induced increase in APP, BACE-1, and presenilin expression levels in the amyloid cascade and decreased BDNF and NGF expression levels in neuronal regeneration.

Response of sesame (Sesamum indicum L.) to foliar-applied thiourea under saline conditions

Salt stress is among the most pervasive limitations for food production, which eventually results in negative financial, environmental and social consequences. Thiourea is a vital chemical which improves growth and yield of plants. Sesame (Sesamum indicum L.) is a crop of semi-arid and arid regions, whose medicinal and seed oil properties are well known. A pot experiment was carried out to examine the impact of thiourea on sesame plants exposed to saline conditions. Two cultivars of sesame, i.e., TH-5 and TH-6 were used. Two levels (0 and 70 mM) of salinity stress as well as two foliar-applied levels (water spray and 150 mM) of thiourea were maintained after 28 and 34 days of seed sowing, respectively. Data for various morphological and physiological attributes was taken after 56 days of seed sowing. The results showed that salinity stress diminished shoot mass along with root mass (fresh and dry) as well as shoot and root lengths. Of physiological parameters, chlorophyll and gas exchange attributes were also negatively affected by the salinity stress. Salinity stress escalated the activities of SOD and POD as well as the levels of MDA. Foliar-applied thiourea raised the contents of chlorophyll b, total chlorophyll and carotenoids of cv. TH-6 under salt stress. The activity of POD of both cultivars under control and salt stress conditions and root K+ concentration of cv.TH-5 under salt stress were also enhanced by thiourea. Overall, foliar application of thiourea mitigated the negative impacts of salinity on sesame.

Agronomic Characteristics and Yield Values of Sesame (Sesamum indicum L.) Cultivars at Various Sowing Dates

In this study, ten different sesame (Sesamum indicum L.) cultivars registered in Türkiye were investigated for their sowing dates as a second crop in the lowlands of Mardin province, in 2018 and 2019. In this study, ten different sesame cultivars were used; these were Arslanbey, Boydak, Cumhuriyet-99, Hatipoğlu, Muganlı-57, Orhangazi-99, Osmanlı-99, Sarısu, Tan-99, and Tanas. Four different sowing dates (June 5, June 15, June 25, and July 4) were examined for these cultivars. In this study, agricultural parameters such as plant height, number of lateral branches, number of capsules, seed yield, oil ratio, and oil yield of the sesame cultivars were investigated. Different sowing dates were found to have a significant effect on the sesame cultivars. The second sowing date resulted in the highest plant height, number of branches, number of capsules, seed yield, oil content, and oil yield (June 15). In terms of cultivars, the Boydak and Sarısu had the highest seed yield (2184 and 2149 kg ha-1, respectively), the Osmanlı-99 (54.6%) had the highest oil ratio, and the Sarısu and Boydak had the highest oil yield (1075 and 1062 kg ha-1, respectively). The Boydak and Sarısu cultivars are promising for high seed and oil yields in the second crop sowing, and a sowing date of June 15 is advised.

Performances of black seed sesame (Sesamum indicum L.) under low fertility saline soils

The world need for healthy cooking oil has readjusted research into other non-conventional oil crops like sesame. The importance of sesame is not only because of its healthy oil crop but also because of its short life cycle making it an attractive cash crop. The crop has been reported to be moderately sensitive to saline-soil and drought. Previous reports on the salinity tolerance were conducted on artificial saline soils with considering fertility thus it is important to evaluate the crops performance on naturally low fertility saline soils. Therefore, the objective of this study was to investigate the effect of saline-soil on growth, development and yield and yield related traits of black seed sesame. The pot experiment was conducted at Buri Ram province Thailand, in a 2 × 6 factorial in RCBD with three replications, where factor A was the soil salinity levels and factor B was the sesame cultivars. Sesame seeds were planted in a seedbed and later were transplanted into the 72 experimental pots each filled with 9 kg soil. Observed results showed that salinity levels had effect on sesame i.e. plant height, number of branches at 30 DAT, SCMR, total chlorophyll content, number of capsules, total dry weight and capsules dry weight. An interaction between salinity level and cultivar was also investigated i.e. number of branches and capsules, total and capsules dry weight and harvest index (HI). Salinity levels had influence on sesame both on growth and yield parameters; cv. Kanchanaburi and KKU2 showed higher number of capsules when compared with other cultivars grown under saline soil, whereas cv. Kanchanburi, UB3 and Buriram gave higher capsules dry weight under saline-soil.

Effect of Foliar Nutrition with Brassinolide on Vegetative Growth Characteristics of Several Sesame Cultivars

In the summer of 2022, researchers in the Karma region of Anbar Governorate applied foliar nutrition with brasinolide to four different sesame cultivars to see how it affected their growth. The study followed a randomized complete block design (RCBD), with a split-plot layout and three replicates. Brassinolide concentrations occupied the main plots (0, 0.5, 1, 1.5 mg L-1), while the cultivars (Sumer, Rafidain, Wadea, Hadd) occupied the sub-plots. The results indicated that the Sumer cultivar recorded the lowest number of days from planting to the beginning of flowering, which amounted to 39.83, it also gave the highest average plant height of 198.17 cm. The cultivar Hadd was superior in the number of branches (6.29 plant-1), the leaf area (7863 cm2 plant-1), and the dry weight of the plant (223.48 gm). Whereas, the Wadea cultivar outperformed in the leaves content of chlorophyll (26.53 mg 100 g-1 fresh weight). The brasinolide concentration of 1.5 mg L-1 recorded the shortest period from planting to the beginning of flowering (38.67 days), plant height (199.17 cm, number of branches 5.08 branch plant-1, leaf area 5619 cm2 plant-1, chlorophyll content (26.93 mg 100 gm-1 fresh weight, and plant dry weight 215.58 gm. There was a significant interaction between the cultivar Sumer and the concentration of 1.5 mg L-1 in the number of days from planting to the beginning of flowering and plant height. Also, there was an interaction between Hadd cultivar and the same concentration in the number of branches, leaf area and dry weight of the plant. While, the cultivar Wadea, it also interacts with the same concentration in the leaf’s chlorophyll content.

Response of Two Sesame (Sesamum indicum L.) Cultivars to Nitrogen and Phosphorus Fertilization under Rain Fed Conditions

A field experiment was conducted for two consecutive seasons(2002/2003 and 2003/2004) in the Experimental Farm of the Faculty ofAgriculture, University of Sinnar, Abu Naama, Sudan. The objective wasto study the response of two sesame (Sesamum indicum L.) cultivars tonitrogen and phosphorus fertilization under rain fed conditions. Thetreatments were four nitrogen levels (0, 40, 80, 120 kgN/ha) and fourphosphorus levels (0, 50, 100, 150 kgP 2 O5 /ha). Urea (46% N) andtriplesuperphosphate (48% P 2 O5 ) were used as sources of nitrogen andphosphorus, respectively. Two improved sesame cultivars, Khidir andPromo, were used in the study. The experiment was laid out in a split-splitplot design with four replicates. Nitrogen treatments were assigned to themain plots, whereas cultivars and phosphorus treatments were allotted tothe sub and sub-sub plots, respectively. The results showed that bothnitrogen and phosphorus had significant effects on plant height, shoot dryweight, height to the first capsule, capsule number/plant, percentages ofcapsules on the main stem and the branches and seed yield. The cultivarsshowed significant differences in some growth and yield attributes, withPromo exhibiting better growth and giving higher yield than Khidir.There was significant interaction between cultivars and nitrogen on seedyield in both seasons

Bacilli Rhizobacteria as Biostimulants of Growth and Production of Sesame Cultivars under Water Deficit.

A strategy using bacilli was adopted aiming to investigate the mitigation of the effects of water deficit in sesame. An experiment was carried out in a greenhouse with 2 sesame cultivars (BRS Seda and BRS Anahí) and 4 inoculants (pant001, ESA 13, ESA 402, and ESA 441). On the 30th day of the cycle, irrigation was suspended for eight days, and the plants were subjected to physiological analysis using an infrared gas analyzer (IRGA). On the 8th day of water suspension, leaves were collected for analysis: superoxide dismutase, catalase, ascorbate peroxidase, proline, nitrogen, chlorophyll, and carotenoids. At the end of the crop cycle, data on biomass and vegetative growth characteristics were collected. Data were submitted for variance analysis and comparison of means by the Tukey and Shapiro-Wilk tests. A positive effect of inoculants was observed for all characteristics evaluated, contributing to improvements in plant physiology, induction of biochemical responses, vegetative development, and productivity. ESA 13 established better interaction with the BRS Anahí cultivar and ESA 402 with BRS Seda, with an increase of 49% and 34%, respectively, for the mass of one thousand seeds. Thus, biological indicators are identified regarding the potential of inoculants for application in sesame cultivation.

Comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis.

Sesame production is severely affected by unexpected drought stress during flowering stage. However, little is known about dynamic drought-responsive mechanisms during anthesis in sesame, and no particular attention was given to black sesame, the most common ingredient in East Asia traditional medicine. Herein, we investigated drought-responsive mechanisms of two contrasting black sesame cultivars (Jinhuangma, JHM, and Poyanghei, PYH) during anthesis. Compared to PYH, JHM plants showed higher tolerance to drought stress through the maintenance of biological membrane properties, high induction of osmoprotectants' biosynthesis and accumulation, and significant enhancement of the activities of antioxidant enzymes. For instance, the drought stress induced a significant increase in the content of soluble protein (SP), soluble sugar (SS), proline (PRO), glutathione (GSH), as well as the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in leaves and roots of JHM plants compared to PYH plants. RNA sequencing followed by differentially expressed genes (DEGs) analysis revealed that more genes were significantly induced under drought in JHM than in PYH plants. Functional enrichment analyses disclosed that several pathways related to drought stress tolerance, such as photosynthesis, amino acids and fatty acid metabolisms, peroxisome, ascorbate and aldarate metabolism, plant hormone signal transduction, biosynthesis of secondary metabolites, and glutathione metabolism, were highly stimulated in JHM than in PYH plants. Thirty-one (31) key highly induced DEGs, including transcription factors and glutathione reductase and ethylene biosynthetic genes, were identified as potential candidate genes for improving black sesame drought stress tolerance. Our findings show that a strong antioxidant system, biosynthesis and accumulation of osmoprotectants, TFs (mainly ERFs and NACs), and phytohormones are essential for black sesame drought tolerance. Moreover, they provide resources for functional genomic studies toward molecular breeding of drought-tolerant black sesame varieties.

Hybrid and native sesame seeds subjected to salinity

Sesame is a plant with great economic and social potential, being used for various purposes such as the food, pharmaceutical, medicinal industries, and more recently, as a source of renewable energy for the production of biofuel. One of the factors that most limit the productivity of this crop is salt stress. Based on this, the present work aimed to evaluate the germination and vigor of hybrid and creoles sesame seeds subjected to saline stress. The experiment was carried out at the Phytotechnics Laboratory of the Engineering and Agricultural Sciences Campus/UFAL. Two sesame cultivars were studied, BRS Anahí and Creole from the state of Sergipe. The seeds were aseptically treated by immersion in 70% alcohol for 1 minute, followed by washing in running water before being subjected to the treatments. To simulate salt stress, sodium chloride (NaCl) was used as a solute at concentrations of 0.0 (control); -0.1; -0.2; -0.3; -0.4 and -0.5 MPa. The material was placed, Biochemical Oxygen Demand (B.O.D.) germination type chamber, regulated at an alternate temperature of 20-30 °C. The experimental design used was completely randomized, with four replications of 25 seeds per treatments in a 2 x 6 factorial scheme (sesame cultivars x saline concentrations). Data were submitted to analysis of variance and polynomial regression, and the analyzes were performed using the SISVAR 5.6 software. The parameters evaluated were: water content, germination, first germination count, germination speed index, seedling length and dry mass. The results for the germination of sesame seeds submitted to salt stress showed that as the salinity levels increased there was a reduction in the percentage of germination of the sesame seeds studied, but until the concentration of -0.4 MPa they are within the standards allowed for commercialization of that culture. In the first count of germination, the Creoles sesame seeds showed the highest values, demonstrating better germination performance compared to BRS Anahí. In the germination speed index, BRS Anahí outperformed Creoles in all salt concentrations, except for the control. For seedling length, it was found that at the lowest salt concentrations both sesame varieties obtained the highest averages, with a significant reduction in length at the highest concentration (-0.5). The dry mass of the seedlings decreased as the water salinity increased, being more evident after the -0.3 MPa NaCl solution, especially for BRS Anahí. Thus, it can be concluded that salinity affects the physiological potential of sesame seeds, being more evident when at salt concentrations from -0.3 MPa. Creoles sesame seeds are less sensitive to saline stress.

Germination and Seedling Growth of Sesame Cultivars under Salinity Stress

Among the most common abiotic stresses that retard the development and growth of plants is salt stress. This study examined the impact of various salt levels (NaCl and Na2SO4) on the germination behaviour of sesame cultivars. Under various simulated salt concentrations (0.05M, 0.01M, 0.15M, 0.2M, 0.25M, 0.3M, 0.35M, 0.4M, and 0.5M), the degree of salinity tolerance of two sesame cultivars, viz., GT-10 and JTS-8 were assessed. The radicle, hypocotyl length, and fresh weight measurements were made separately after 96 and 144 hours. The results revealed that germination and seedling growth drastically decreased as the salt concentration increased. In comparison to GT-10, JTS-8 exhibits greater tolerance to different NaCl concentrations and greater sensitivity to varying Na2SO4 concentrations.

Assessment of Novel Genetic Diversity Induced by Mutagenesis and Estimation of Genetic Parameters in Sesame M4 Mutant Lines

Sesame (Sesamum indicum L.) is an ancient oilseed, aromatic, and medicinal crop widely used for its high-quality oil and seeds. The available genetic diversity in Morocco is too limited; thus, a mutagenesis breeding program was adopted. This study was carried out to evaluate the novel variability induced and observed in 11 M4 mutant lines and to estimate some valuable genetic parameters. The experiment was conducted in two different environments using a randomized complete block design with three replications. Phenological, morphological, and agronomic traits were recorded. To estimate the effect of genotype, environment, and their interaction, ANOVA and planned contrast analyses were performed. To examine relatedness among genotypes, cluster analysis was performed. Significant differences among mutants and between parent cultivars and their respective mutant lines were observed. Genetic parameters such as genotypic (GCV) and phenotypic (PCV) coefficients of variation, broad-sense heritability (H2 b.s), genetic advance (GA), and genetic advance over the mean (GAM) were high in most traits. Highly productive mutants, ‘US2-1’ and ‘US1-2’, were observed, exhibiting the highest number of capsules per plant and seed yield ever reported. Additionally, there are other promising mutants with early flowering, early maturity, and a reduced height of the first capsule. This suggests that mutagenesis can be successfully applied to develop high-yielding sesame varieties along with other improved phenological and agromorphological traits. All these mutant lines can be used as promising germplasm to develop competitive sesame cultivars to meet the increasing demand for sesame oil and seeds in the actual context of climate change.

Estimates of Variance Components and Heritability of Seed Yield and Yield Components in Commercial Sesame Cultivars at Different Levels of Irrigation and Mycorrhizal Fungi

Estimates of Variance Components and Heritability of Seed Yield and Yield Components in Commercial Sesame Cultivars at Different Levels of Irrigation and Mycorrhizal Fungi

Genetic diversity using biochemical, physiological, karyological and molecular markers of Sesamum indicum L.

The genetic diversity and the relationships among sesame cultivars were investigated using physiological and cyto/molecular analysis. To our information, no studies have yet been conducted on the genetic evaluation of sesame genotypes based on cyto/molecular analysis in Saudi Arabia. This study showed that genotype Bah-312 had the highest values from physiological and biochemical traits (plant height, harvest index, total plant dry matter, seed yield, oil content, and fatty acids content). Using 20 ISSR and 25 SCoT primers, the studied genotypes amplified 233 and 275 alleles, while the average polymorphism percentage (P%) was 65.32% (ISSR) and 77.8% (SCoT) across all the studied genotypes, respectively. To assess the markers efficiency analysis the polymorphism information contents (PIC), Marker Index (MI), Effective Multiplex Ratio (EMR), Resolving Power (Rp) were estimated. In general, primers (ISSR 2 & SCoT 21) and (ISSR 4 & SCoT 3) revealed the highest and lowest values for P %, PIC, MI, and EMR%. Furthermore, 188 positive and negative unique bands were detected, out of which ISSR generated 84, while 104 were amplified by SCoT analysis. In this regard, genotype Bah-312 generated 41 unique amplicons, and Jiz-511 genotype 23 unique amplicons. In the same context, the population genetics parameters, number of different alleles (Na), number of effective alleles (Ne), Shannon’s index (I), expected heterozygosity (He), and Unbiased Expected Heterozygosity (uHe), were calculated. ISSR marker showed the highest values for all the estimated parameters. In this regard, genotype Bah-312 exhibited the highest values (1.35, 1.37, 0.31, 0.21, 0.29) & (1.31, 1.35, 0.30, 0.20, 0.27) while, genotype Ahs-670 revealed the least values (1.29, 1.31, 0.26, 0.16, 0.23) &(1.14, 1.26, 0.22, 0.15, 0.20) for ISSR and SCoT markers respectively. For cytological data, according to the highest asymmetry index (AsK%) and lowest total form percentage (TF%) values, genotype Ahs-670 was the most advanced cultivar, and genotype Bah-312 was the most primitive one. According to the degree of asymmetry of karyotype (A) and intrachromosomal asymmetry index (A1), sesame genotype Ahs-670 was the most asymmetrical, and Bah-312 was the most symmetrical genotype. This study gives some helpful information about the genetic diversity of six sesame landraces. The variation harbored by these landraces could be used in sesame breeding programs.

Effect of Organic and Phosphate Fertilizers on Yield Traits of Several Sesame Cultivars, Sesamum Indicum L.

Effect of Organic and Phosphate Fertilizers on Yield Traits of Several Sesame Cultivars, Sesamum Indicum L.

Performance of sesame (Sesamum indicum L.) cultivars under different plant population during summer season

An experiment on performance of sesame (Sesamum indicum L.) cultivars under different plant populations during summer season (February to March 2021) was conducted at the Experimental Farm of Centurion University of Technology and Management, Paralakhemundi, Odisha, on sandy loam soil in split plot design with three varieties (YLM 66, YLM 17 and TIL1) in main plots and four plant populations (30 cm x 10 cm, 30 cm x 15 cm, 30 cm x 20 cm and 30 cm x 25 cm) in sub-plots. There were no significant differences in plant height among varieties and spacing. At 60 days after sowing (DAS), significantly higher LAI was obtained with YLM 66 and dry matter with TIL 1 over other varieties. These parameters were higher at 30 cm x 20 cm spacing over other spacings. Number of seeds per capsule (43.8) and seed weight per capsule were significantly higher with YLM 66 as compared to other varieties. However, these parameters did not vary significantly due to spacing. Significantly higher seed yield was observed with variety YLM 66 over other varieties under test. The seed yield did not vary significantly due to spacing. The straw yield was significantly higher in TIL 1 over YLM 17 and YLM 66) and biological yield was higher in TIL 1 and YLM 66 over variety YLM 17. These parameters increased significantly with increase in spacing.

Performance evaluation of commercial sesame (Sesamum indicum L.) cultivars for yield and yield related traits in Wolaita, southern Ethiopia

Sesame (Sesamum indicum L.) is cultivated in Ethiopia as a cash crop, export commodity, and raw material for sesame oil industries. It has surged up as a silver line regarding its contribution to the export earnings for Ethiopians. Sesame cultivation was not widely known in Wolaita area although the area could be a potential cultivation location for wider production of sesame in the country. Therefore, to evaluate performance in terms of yield and yield related traits for selection of the best performing cultivars to enhance productivity and larger production, ten sesame cultivars were laid out in a randomized complete block design with three replications at two districts (Humbo and Kindo-Koysha) for two years (2017-2018). Analysis of variance showed that cultivars Mehado-80 (154 cm), Adi (153 cm), and Tate (149 cm) have grown to significantly higher heights over the rest of the cultivars. Mehado-80 and Tate had a significantly higher number of primary branches (mean values across locations 9.3, 8.7 and 8.6, respectively). The cultivar Tate had the highest number of capsules (97.9) per plant, which had also the highest genotypic variation (428.54). The highest genotypic coefficient of variance (45.58%) was recorded for seed yield. Days to 50% flowering and 90% maturity exhibited the lowest phenotypic coefficient of variation, 7.16%, and 3.69%, respectively. Heritability in a broad sense (h2b) ranged from 13.32% for plant height to 32.43% for thousand seed weight. The interaction effects had showed no significant difference (P>0.05) for most of the studied traits. Cultivars Tate (1200 kg/ha), Serkamo (917 kg/ha) and Kelafo-74 (875 kg/ha) scored significantly higher seed yield at Kindo-Koysha site whereas Tate (1208 kg/ha, S (1042 kg/ha) and Mehado-80 (1021 kg/ha) scored significantly higher seed yield at Humbo site. Hence these cultivars could, respectively, be used as a potential cultivar for Kindo-Koysha and Humbo districts of Wolaita zone, while cultivar Tate exhibited the highest seed yield at both locations and hence recommended for Wolaita zone, southern Ethiopia. Key words: Commercial cultivars, Seed yield, Sesame, Traits, Wolaita, Yield components

Comparative physiological and transcriptomic analysis of sesame cultivars with different tolerance responses to heat stress.

The online version contains supplementary material available at 10.1007/s12298-022-01195-3.

Triple test cross analysis for seed yield and its components in sesame under water stress conditions

Water deficit is a limiting factor in sesame cultivars yield, thus identification of genetic mechanisms of sesame traits under water stress conditions is essential to development of water stress tolerant genotypes. Hence, the triple test cross (TTC) analysis in F2 population of the sesame cross (NA76 ​× ​NA54) was used. Since, 30 TTC families with their 13 respective parents were evaluated during the summer season 2020 under two irrigation treatments. i.e., normal irrigation with 5952.38 ​m3 ​hm−2 of applied water, and water stress with 2976.19 ​m3 ​hm−2 of applied water. This was implemented at the experimental field of Kafr El Hamam/Sharkia, Agricultural Research Station, Agricultural Research Center (ARC), Egypt. Additive (D) and dominance (H) gene actions as well as epistatic and its two components of additive ​× ​additive, additive ​× ​dominance plus dominance ​× ​dominance were involved in the inheritance of all studied traits under both irrigation treatments and their combined analysis. The degree of dominance (H/D)0.5 verified the presence of partial dominance in most cases. The correlation coefficient between sums (additive) and differences (dominance) was non-significant, showing that dominant genes between lines were ambidirectional. The moderately water stress tolerant TTC families as the best selected families were found in families 22, 25, 10, 12, and 15. Therefore, postponing selection in these selected families to later segregating generations for all studied traits would be effective to exploit the positive effects of additive ​× ​additive epistasis.