Wet root rot caused by Rhizoctonia solani Kuhn in pulse crops was favored by wide range of soil parameters like temperature, humidity, pH, electric conductivity and soil texture. The areas surveyed for the collection of the isolates showing variable atmospheric temperature and relative humidity and low to medium levels of soil organic carbon and high level of available phosphorus influenced the development of the disease incidence from 2-48%. Seventy three cultivars of mungbean, twenty eight cultivars of urdbean and eight cultivars of cowpea were evaluated against virulent isolate of R. solani (RASC 30) to design a set of differential cultivars for virulence analysis. Two cultivars of urdbean, namely, NDU3-4 and IPU2-14, one cultivar of mungbean, namely, HUM 1 and three cultivars of cowpea, namely, V240, V585 and DCP7 showed resistant reactions. Four cultivars of urdbean, namely, TU94-2, KU323, KUG216 and B3-8-8, one cultivar of mungbean, namely, PDM54 and two cultivars of cowpea, namely, V578 and DCP13 were moderately resistant against the pathogen. The virulence analysis of 90 isolates of the pathogen representing 7 anastomosis groups (AGs) isolated from pulse crops of 16 agro-ecological regions of India on a set of differential cultivars, namely, HUM 1, PDM 54 and Pusa Vishal of mungbean, NDU 3-4, KU 323, Uttara of urdbean and V 240, V 578 and Pusa Sukomal of cowpea grouped the isolates into five pathotypes. The differential cultivar for each pathotype was identified. The pathotypes were not corresponding to the AG type of the isolates. Except one pathotype (isolate RMPG28 belonging to AG2-3), each pathotype had the isolates from different AGs.