The response of drip-irrigated rice physiological traits to water and fertilisers has been widely studied. However, the responses of yield, root traits and their plasticity to the nitrogen environment in different nitrogen-efficient cultivars are not fully understood. An experiment was conducted from 2020-2022 with high nitrogen efficient (high-NUE) cultivars (T-43) and low-NUE cultivars (LX-3), and four nitrogen levels (0, 150, 300, and 450 kg ha-1) under drip irrigation in large fields were used. The aim was to study the relationship between root morphology, conformation, biomass, and endogenous hormone content and yield and nitrogen use efficiency. The results showed the following: 1) Under the same N application rate, compared with LX-3, T-43’s yield, N partial factor productivity (PFP), fine root length density (FRLD), shoot dry weight (SDW), root indole-3-acetic acid (IAA), and root zeatin and zeatin riboside (Z+ZR) were significantly increased by 11.4-18.9%, 11.3-13.5%, 11.6-15.7%, 9.9-31.1%, 6.1-48.1%, and 22.8-73.6%, respectively, while the root-shoot ratio (RSR) and root abscisic acid (ABA) were significantly decreased (P<0.05). 2) Nitrogen significantly increased rice root morphological indexes and endogenous hormone contents (P<0.05). Compared to N0, yield, root length density (RLD), surface area density (SAD), Root volume density (RVD), and root endogenous hormones (IAA, Z+ZR) were significantly increased in the 2 cultivars under N2 by 61.6-71.6%, 64.2-74.0%, 69.9-105.6%, 6.67-9.91%, 54.0-67.8%, and 51.4-58.9%, respectively. Compared with N3, the PFP and N agronomic efficiency (NAE) of nitrogen fertiliser under N2 increased by 52.3-62.4% and 39.2-63.0%, respectively. 3) The response of root trait plasticity to the N environment significantly differed (P<0.05). Compared with LX-3, T-43 showed a longer root length and larger specific surface area, which is a strategy for adapting to changes in the nutrient environment. For rice cultivars with high-NUE, the root-shoot ratio was optimised by increasing the FRLD, root distribution in upper soil layers, and root endogenous hormones (IAA, Z+ZR) under suitable nitrogen conditions (N2). An efficient nutrient acquisition strategy can occur through root plasticity, leading to increased yield and NUE.
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