Common Bean Cultivars Research Articles

QTL mapping and identification of genes associated with the resistance to Acanthoscelides obtectus in cultivated common bean using a high-density genetic linkage map

BackgroundCommon bean (Phaseolus vulgaris L.) is an important agricultural product with large nutritional value, and the insect pest Acanthoscelides obtectus (Say) seriously affects its product quality and commodity quality during storage. Few researches on genes of bruchid resistance have investigated in common bean cultivars.ResultsIn this study, a bruchid-resistant cultivar black kidney bean and a highly susceptible accession Longyundou3 from different gene banks were crossed to construct a recombinant inbred line population. The genetic analysis indicated a quantitative inheritance of the bruchid resistance trait controlled by polygenes. A high-density genetic map of a total map distance of 1283.68 cM with an average interval of 0.61 cM between each marker was constructed using an F6 population of 157 recombinant inbred lines. The map has 3106 bin markers, containing 2,234,769 SNPs. Using the high-density genetic map, a new quantitative trait locus for the resistance to Acanthoscelides obtectus was identified on chromosome 6. New molecular markers based on the candidate region were developed, and this locus was further delimited to an interval of 122.3 kb between SSR markers I6–4 and I6–16 using an F2 population. This region comprised five genes. Phvul.006G003700, which encodes a bifunctional inhibitor, may be a potential candidate gene for bruchid resistance. Sequencing analysis of candidate gene identified a 5 bp insertion-deletion in promoter of gene Phvul.006G003700 between two parents. Expression analysis of candidate gene revealed that the expression level of Phvul.006G003700 in bruchid-resistant parent was markedly higher than that in bruchid-susceptible parent both in dry seeds and leaves.ConclusionsA high-density genetic linkage map was constructed utilizing whole-genome resequencing and one new QTL for bruchid resistance was identified on chromosome 6 in common bean cultivar. Phvul.006G003700 (encoding a bifunctional inhibitor) may be a potential candidate gene. These results may form the basis for further research to reveal the bruchid resistance molecular mechanism of common bean.

Alternatives for selection of carioca common bean lines that combine upright plants and slow grain darkening

In Brazil, there is an increasing demand for common bean (Phaseolus vulgaris L.) cultivars having Carioca-type (cream-colored seed coat with brown stripes) beans which have a light color that persists for a longer time combined with the most possible upright plant architecture. However, plant breeders are finding it difficult to combine these two traits in the same cultivar. The aim of this study was to compare success in the selection of new common bean lines that combine the two traits in segregating populations with 25%, 50%, or 75% of the alleles from the parents contrasting in upright plant architecture or in bean grains with persistent light color. A biparental cross was performed between a parent with light-colored beans (P1) and another with upright plants (P2). Three populations were obtained from the F1 seeds, with allelic frequencies of 75%, 50%, and 25% of P1. Subsequently, progenies were evaluated in a similar number in each population in the F2:3, F2:4, F2:5, and F2:6 generations. The plant architecture (PA) and grain darkening (GD) traits were visually evaluated using a scoring scale. Genetic and phenotypic parameters were estimated for all generations. There was no expressive advantage in using populations with a greater allele proportion of the parent favorable to upright plant architecture or slow grain darkening either in releasing of variability or in the occurrence of progenies with favorable phenotypes for the two traits.

Foliar-Applied Seaweed Extract Improves Yield of Common Bean (Phaseolus vulgaris L.) Cultivars Through Changes in Biochemical and Fatty Acid Profile Under Irrigation Regimes

Foliar-Applied Seaweed Extract Improves Yield of Common Bean (Phaseolus vulgaris L.) Cultivars Through Changes in Biochemical and Fatty Acid Profile Under Irrigation Regimes

​​Evaluation of Dry Bean Cultivars in Turkey for Resistance against Common Bacterial Blight Disease

Background: Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. phaseoli (Xap) has been recorded as one of the most important seed-borne destructive diseases of beans, affecting its production worldwide. Since chemical compounds cause serious damage to natural ecosystems and in some instances fail in controlling CBB, the use of genetically resistant cultivar is the most economically efficient and environmentally friendly measure in the control of CBB disease. The objective of this study was to assess the resistance reactions of widely grown and popular common bean cultivars against Xap under controlled conditions. Methods: In this study, 16 dry bean cultivars comprising eight widely grown dry bean varieties, six improved and promising genotypes and two known CBB susceptible varieties, were evaluated for their reaction to the CBB in a completely randomized design with three replications. The reaction of the bean cultivars to Xap was assessed using disease incidence, disease severity, per cent severity index, the area under disease progress curve (AUDPC) and incubation period. Result: Data analysis indicated that there were highly significant differences (p≤0.01) among common bean cultivars for CBB disease incidence, disease severity, per cent severity index and AUDPC among cultivars. The cultivated varieties Noyanbey and Özmen were identified as resistant cultivars. In addition, SAP6 and BAC6 resistance genes were found in these two cultivars. As a consequence, it was thought that these cultivars could be a potential source of disease resistance for CBB in breeding programs and sustainable, eco-friendly and good agricultural practices.

What are the impacts of water deficit, cultivars, and years on the dynamics of nutrient uptake by common bean? Part II: Ca, Mg, and S

We aimed to explain and compare Ca, Mg, and S uptake dynamics by two common bean cultivars with contrasting growth habits as a function of water deficit severity in two years to generate more specific information for their fertilization management. A field study was set up using the cultivars IAC Imperador, with a determinate growth habit and early cycle, and IPR Campos Gerais, with an indeterminate growth habit and intermediate cycle. These cultivars were maintained under three irrigation levels, that is, severe (54% ETc), moderate (77% ETc), and no water deficit (100% ETc). The absorption order was Ca > Mg > S, regardless of the treatment. For the IAC Imperador, the Ca uptake ranged from 72 to 127 kg ha−1, Mg from 17.2 to 39.1 kg ha−1 and S from 7.9 to 16.5 kg ha−1, while for the IPR Campos Gerais it ranged from 92 to 156 kg ha−1, 21.0 to 36.5 kg ha−1 and 14.6 to 33.7 kg ha−1, respectively. Overall, severe water deficit reduced Ca, Mg, and S absorption by more than 25%. The IPR Campos Gerais presents, on average, 64% higher S accumulation, while the highest Ca and Mg accumulation order depends on the year. Compared to the IAC Imperador, this cultivar showed a later S absorption peak (up to +23 days), and higher interannual variability in the growth and nutrient absorption due to its indeterminate growth habit. These differences evidence the importance of specific fertilizer management practices for Ca, Mg and S in common bean, assisting in optimizing nutrient use.

Identification of Causal Agent Inciting Powdery Mildew on Common Bean and Screening of Resistance Cultivars

Powdery mildew is one of the severe diseases on common bean in Southwestern China, but the identity of the pathogen inciting this disease is unclear. The objective of this study was to identify the causal agent of common bean powdery mildew and to screen resistant cultivars. The pathogen was identified through morphological identification, molecular phylogenetic analysis, and pathogenicity tests. Resistance of common bean cultivars was evaluated by artificial inoculation at the seedling stage. The common bean powdery mildew isolate CBPM1 was obtained after pathogen isolation and purification. Morphological identification confirmed that the isolate CBPM1 belonged to the Oidium subgenus Pseudoidium and germinated Pseudoidium-type germ tubes. Molecular phylogenetic analysis showed that the isolate CBPM1 and Erysiphe vignae isolates from different hosts were clustered into a distinct group. The pathogenicity and host range tests revealed that the isolate CBPM1 was strongly pathogenic to common bean, multiflora bean, lablab bean, cowpea, and mung bean, but not to soybean, adzuki bean, pea, faba bean, chickpea, lentil, pumpkin, and cucumber. In addition, 54 common bean cultivars were identified for resistance to powdery mildew, and 15 were resistant or segregant. Based on the morphological, molecular and pathogenic characteristics, the causal agent of common bean powdery mildew was identified as E. vignae. This is the first time E. vignae has been confirmed on common bean. Cultivars with different resistance levels were screened, and these cultivars could be used for disease control or the breeding of new resistant cultivars.

Agronomic biofortification increases concentrations of zinc and storage proteins in common bean (Phaseolus vulgaris L.) grains

Zinc (Zn) is an essential element for plants and mammals and its deficiency affects billions of people worldwide. This study aimed to evaluate the effects of soil Zn fertilization and foliar Zn sprays in different phenological stages of the plant on the grain nutritional quality of common bean (Phaseolus vulgaris L.). Field experiments were carried out in two consecutive harvest years under no-till system in an Oxisol. Two commercial common bean cultivars were used: BRS Esteio (black bean) and IPR Campos Gerais (Carioca bean). Total concentration of Zn, amino acids, sucrose, total sugars and storage proteins (albumin, globulin, glutelin and prolamin) in grains were evaluated. The cultivar BRS Esteio exhibited higher grain enrichment with Zn than the cultivar IPR Campos Gerais, showing genotypic variation. Single foliar Zn spray of 600 g ha−1 at the initial grain filling stage was shown to be the best way to improve the grain Zn concentration, without affecting grain yield. Foliar Zn spray at the final stage of grain filling favored the increase of Zn concentration in the pods over the Zn concentration in the grains. Agronomic biofortification of bean grains with Zn was more efficient with foliar Zn spraying than with the soil Zn fertilization, however, the soil Zn application favored the increase of concentrations of total amino acids and storage proteins (albumin, globulin and glutelin) in the grains. Agronomic biofortification proved to be efficient in increasing concentrations of Zn and storage proteins in common bean grains.

Influence of common bean genotypes and rhizobia interaction for nodulation and nitrogen fixation

Bacteria from the rhizobia group are able to associate symbiotically with bean crop, forming nodules in the root, in which the biological nitrogen-fixing process occurs. However, the efficiency of this process has been low and it can be attributed to genetic and environmental factors. Thus, the objective of this study was to evaluate the capacity of nodulation of local varieties and commercial common bean cultivars inoculated with a Rhizobium tropici strain used in commercial inoculants and rhizobia isolates from common bean root nodules. The experiment was carried out in a factorial scheme (2x4), in a randomized block design with four replicates. It was tested two local varieties and two commercial cultivars, inoculated with the reference Rhizobium tropici strain CIAT899 and the RBZ14 strain isolated from common bean nodules grown in soils of Southern Brazil, in adapted Leonard-type pots. The CIAT899 strain promoted either higher mass of viable nodules and higher nitrogen accumulation in the aerial part. The black group local variety showed better response than the cultivar TAA Dama for nodule viability, suggesting more efficiency for nodulation. The interaction between genotypes (local varieties and commercial cultivars) and bacteria showed the specificity of the complex symbiotic relationship of biological nitrogen fixation in common bean, requiring further studies of these interactions.

Chemical and morphological characteristics of common bean seed and evaluating genetic advance in commercial classes.

Common bean (Phaseolus vulagris L.) is a nutritionally important food crop with prospective health benefits in the world. The current study was evaluated the chemical components, morphological characteristics, and genetic advance of 22 common bean cultivars/lines seeds from three commercial classes (white, red, pinto beans) adapted to different climates of Iran. The results showed significant variations among 22 common bean cultivars/lines for all studied seed traits. The commercial group comparisons showed that pinto beans were the best in terms of seed morphological characteristics but red beans were superior for seed protein percentage and zinc content. White beans had high amounts of iron, calcium and magnesium, and also presented high amounts of starch and uronic acid as anti-nutritional factors. Among the chemical components, crude fat had the highest genetic and phenotypic coefficients of variation, whereas starch percentage showed the lowest values. The genetic advance over the mean ranged from 6.73% (starch percentage) to 66.31% (100-seed weight), and high heritability was estimated for calcium content (0.99). AND1007 Line demonstrated the high seed protein, iron and zinc contents. To confirm the results, a genotype-by-trait biplot was done. These results could help to achieve a common bean cultivar with a high amount of nutritional value of seeds and appropriate seed characteristics with a low amount of anti-nutritional factors.

Protein profile and antioxidant capacity of processed seeds from two common bean (Phaseolus vulgaris L.) cultivars

AbstractCommon bean (Phaseolus vulgaris L.) seeds are an economical protein source rich in bioactive compounds with antioxidant activity. In this work, we evaluated processed seeds from two common bean cultivars regarding protein profile and antioxidant capacity before and after enzymatic digestion in vitro. We provided protein maps by two‐dimensional electrophoresis (2‐DE) of seeds germinated during 36 h and 72 h. The principal component analysis (PCA) showed that protein abundance had more variation on germination times than the cultivar ones. Seeds germinated for 36 h showed greater antioxidant capacity compared to germination for 72 h and cooking, before and after enzymatic digestion. In protein isolates, cooking associated with digestion provided better antioxidant capacity. Germination time influences protein expression as well as the antioxidant capacity of common beans. Furthermore, in vitro digestion increases the antioxidant capacity of cooked bean protein isolates.

Demanda nutricional e exportação de nutrientes por cultivares modernas de feijão-comum

Abstract The objective of this work was to quantify the extraction and export of nutrients by common bean (Phaseolus vulgaris) cultivars from different commercial groups and with different growth habits. The experiment was conducted using four common bean cultivars: TAA Gol, BRS FC104, IPR Tuiuiú, and TAA Dama. Total dry matter (DM) and nutrient accumulation in the V4 (with four, six, and eight three-leaflet leaves), R5, R7, R8, and R9 stages was determined, as well as grain yield and nutrient export. Differences were observed both in nutrient uptake and in DM and nutrient accumulation among the cultivars in the initial stages of development (V4 with six and eight three-leaflet leaves). The TAA Gol cultivar showed a greater accumulation of almost all nutrients in these two initial stages. In R7, the cultivar that had the greatest DM and nutrient accumulation was TAA Dama, but, from R8 onwards, there were no significant differences. Although nutrient export is similar among the evaluated cultivars, a lower percentage is translocated to the grain in TAA Dama.

Simbiozna učinkovitost autohtonih sojeva rizobija na grahu izoliranih iz sjeverozapadne Hrvatske

The strains of nodule bacteria differ greatly in their symbiotic properties and efficiency in nitrogen fixation and therefore, the selection of highly effective strains is of great importance when attempting to achieve a successful pre-sowing inoculation of common bean. The aim of this study was to determine the nodulation ability of indigenous rhizobial strains and to evaluate their compatibility and symbiotic efficacy with two common bean cultivars. A two-factorial pot experiment (12 indigenous rhizobial strains and two common bean cultivars) was set up in the greenhouse. In the full blossom phase, dry nodule biomass, shoot dry weight and N content in dry shoot was determined. The research revealed significant differences depending on the strain of nodule bacteria and common bean cultivars. The compatibility of indigenous and reference strains on the examined common bean cultivars was also observed. The cultivar Tresnjevac was significantly better than Slavonski zeleni in all measured parameters. The results revealed that the indigenous common bean rhizobial strains considerable differ in their symbiotic efficiency. The highest dry nodule biomass was determined when strain HO12 was applied, and the highest shoot dry weight with strains MB1 and DE7. The application of strain DE7 revealed the highest N content in dry shoot. The results indicate that these indigenous strains of common bean nodulating bacteria are characterized by the highest symbiotic efficiency and could be used for the pre-sowing inoculation of common bean after testing in field scale experiment.

Reinoculação de Rhizobium tropici em cobertura combinado ou não com Azospirillum brasilense em feijoeiro

ABSTRACT The nitrogen (N) supplying capacity of common bean plants through biological fixation is still questioned by farmers. This study aimed to investigate the nodulation, growth and production of common bean cultivars under field conditions, when subjected to topdressing Rhizobium tropici reinoculation applied at different rates, combined or not with Azospirillum brasilense. A randomized blocks design was used, in a 2 × 4 × 2 + 2 factorial arrangement, with four replications. The treatments consisted of two common bean cultivars (BRS-Estilo and BRS-Esteio) reinoculated with four R. tropici rates (0-, 1-, 2- and 4-fold the reference rate), combined or not with A. brasilense in co-inoculation. The additional treatments consisted of nitrogen fertilizer applications (20 and 50 kg ha-1 at planting and as topdressing, respectively) for both cultivars. The variables evaluated at the R6 stage were nodulation (number of active nodules and nodule dry weight) and morphological plant characteristics (root length and dry weight, plant height, shoot dry weight and N content), while, at harvest, the number of pods per plant, number of grains per pod, 100-grain average weight and grain yield were evaluated. The topdressing reinoculation of R. tropici combined with A. brasilense at the V4 stage affected the nodulation process and agronomic characteristics of the common bean plants. When performed in addition to seed inoculation, it may totally replace the supply of mineral N in the crop, allowing the achievement of high yield levels.

Selection strategies for identifying fast cooking, mineral-biofortified bean cultivars with high agronomic performance

Simultaneous selection for various agronomic traits, cooking time and mineral concentration are major challenges for common-bean (Phaseolus vulgaris L.) breeding programs. The authors of this study proposed to analyze genetic gain estimates obtained by direct and indirect selection using selection indices and economic weights for 13 traits, and to determine the most efficient selection strategy for the simultaneous selection of fast cooking, mineral-biofortified common bean cultivars with high agronomic performance. For this purpose, three experiments were carried out in different growing seasons to evaluate 49 common bean cultivars of different grain types. Agronomic performance was evaluated based on six traits; cooking time was determined using a Mattson cooker; and the concentration of six minerals was analyzed in samples of raw grains. Significant genotype × environment interaction or genotype effects were observed for all traits, indicating the existence of genetic variability. Direct selection resulted in high genetic gain estimates for individual traits, but caused undesirable changes in one or more of the traits under selection. The classic, base, desired-gains and rank-sum selection indices tested with six economic weights do not provide genetic gain estimates favorable to the selection of all traits. The multiplicative index is the best selection strategy for use in the breeding program when aiming at the simultaneous selection of fast cooking, mineral-biofortified common bean cultivars with high agronomic performance.

Association mapping for common bacterial blight in carioca beans

Common bacterial blight (CBB) is an important disease in Phaseolus vulgaris L. A carioca diversity panel (CDP) composed of 149 cultivars of common bean was genotyped with 1,616 SNPs and evaluated for Xanthomonas phaseoli pv. phaseoli (Xap) resistance aiming to identify QTLs. Phenotypic evaluation was done in controlled conditions. The plants displayed in a randomized block design, with 3 replications were evaluated ten days after inoculation. GWAS analysis using the BLINK model identified one SNP on chromosome Pv07, with a different genomic location in relation to previous studies. Considering a confidence interval of 100 kb, gene annotation identified 13 candidate genes related to defense-associated genes, and their key functional motives were discussed. The marker identified may constitute an important resource for future marker assisted CBB resistance breeding.

Assessment of the technological quality characters and chemical composition for some Egyptian Faba bean germplasm

Increasing the yield and nutritional value of faba beans is one of the main objectives of the common bean cultivar improvement programs due to its contribution to terminate protein deficiency malnutrition in developing countries and raising the income level of smallholder farmers. In this study, the technological quality of bean seeds from twenty-four genotypes were evaluated (twenty germplasm and four improved cultivars (Misr 1, Giza 429, Giza 716, Giza 843). The results showed a significant diversity in the Egyptian bean germplasm in their quality traits compared to the control cultivars and indicated that the genotypes had a high hydration coefficient that ranged between (98.6-112.4%). While most of the genotypes for cotyledons to hull ratio met the commercial criteria that ranged between (7.41-6.41%). Twenty genotypes had the highest total soluble solids (6.90-11.78%) and were superior to the control cultivars. The seeds chemical composition analysis showed that the genotypes differed in their composition such as protein (26.65 to 30.72 %.), carbohydrate (58.00 to 62.25%), tannin (70.67 to 157.45%), phenols (35.23-51.02 %), and moisture (9.15 to 10.45%).

Snap Bean Breeding for Rust Resistance: Validation of Molecular Markers for The Ur-11 Gene Introgression

Bean rust caused by <em>Uromyces appendiculatus </em>fungus is one of the most destructive diseases reported in temperate<em>,</em> <em>tropical, </em>and sub-tropical regions, affecting the quality and quantity of <em>common </em>bean (Phaseolus vulgaris <em>L) </em>yield. The present study aimed to improve the breeding process of snap beans for rust resistance by identification of inheritance patterns of resistant genes using phenotypic and molecular markers. Among the different genes identified, Ur-11 has wide-spectrum resistance to 89 out of 90 reported races of the rust pathogen. Therefore, the common bean cultivar PI 181996, having the Ur-11 gene was used as the rust resistance donor parent while the local popular <em>snap bean </em>variety <em>Kappetipola nil</em>, susceptible to all locally reported races of the rust pathogen in Sri Lanka, was selected as the susceptible (Recurrent) parent. The introgression of the Ur-11 gene from the cultivar PI 181996 in F1, F2, and BC1F1 populations were phenotypically evaluated using standard screening method while validating random amplified polymorphic DNA (RAPD) marker, OPAC 20 and sequence characterized amplified region (SCAR) marker, SAE 19 and GT 2. Coupling markers (OPAC 20 and GT-2) and repulsion marker (SAE 19) showed 95% and 100% of expected results for phenotypically resistant and susceptible lines respectively. The results showed that the Ur-11 gene in PI 181996 can be tagged with SCAR marker GT-2 and SAE 19 and can be used for markerassisted selection to identify the presence of the Ur-11 gene in the snap bean rust-resistance breeding program. Introgression of the Ur-11 gene helped to develop genetic resistance to local races of the rust pathogen, and the selected progenies could be developed as new resistant snap bean breeding lines.

Registration of the slow‐darkening pinto common bean cultivar ‘Wildcat’

AbstractSlow‐darkening pinto common bean (Phaseolus vulgaris L.) cultivar ‘Wildcat’ (Reg. no. CV‐345, PI 698190) was released by the University of Nebraska dry bean breeding program in 2018. It was bred for adaptation to western Nebraska growing conditions and enhanced resistance to bean rust, common bacterial blight (CBB), and Bean common mosaic virus (BCMV). The yield of Wildcat was comparable to conventional and slow‐darkening pinto bean cultivars grown in Nebraska from 2015 to 2020. During the 2017 Western Regional Bean Trial, 2017 Midwest Regional Performance Nursery, and 2018 Cooperative Dry Bean Nursery, Wildcat had a higher seed yield than ‘Twin Falls’, ‘Nez Perce’, ‘Black Foot’, ‘Montrose’, ‘Buster’, ‘ND‐Palomino’, and ‘La Paz’ at some locations. Wildcat has the Ur‐11 gene that confers resistance to all but one known bean rust race and carries the Indel NDSU_IND_11_48.459.896 marker tagging the Ur‐11 locus. Wildcat also has the SAP6 sequence characterized amplified region (SCAR) marker linked to a major CBB resistance quantitative trait locus, with an intermediate CBB rating (5.0) under field conditions at western Nebraska in 2020. Wildcat also carries the single dominant I gene that confers resistance to all non‐necrotic strains of BCMV and the SW13 SCAR marker linked to the I gene for resistance to BCMV. Wildcat has white flowers, blooms 46 d after planting, and is a full‐season bean, maturing 93 d after planting. The seed size of Wildcat, 43.3 g 100 seeds–1 on average, was significantly larger than current conventional and slow‐darkening pinto beans.

What are the impacts of the common bean production system on production and quality attributes of the grains?

The choice of a cultivar can significantly affect grain yield and quality of common bean crops in production systems. The objective of this work was to evaluate the effect of common bean cultivars of the carioca commercial group on grain yield and quality in a production system, and assess the correlation between their variables. The experiment was carried out in the Southeast region of Brazil, in the winter-spring crop season. A randomized block design was used, with 13 treatments and four replications per treatment. The treatments were consisted of common bean cultivars (Pérola, BRSMG-Majestoso, BRS-Estilo, BRSMG-Madrepérola, IPR-Campos Gerais, IPR-Tangará, IPR-Andorinha, IPR-139, IAC-Imperador, IAC-Formoso, IAC-Alvorada, IAC-Milênio, and TAA-Bola Cheia). The choice of a cultivar affects production and quality attributes of common bean in production systems, as it can result in increases of up to 34% in grain yield, 20% in grain yield in sieves equal to or higher than 12 (GYS≥12), 16% in grain protein contents, and 47% in cooking time. The cultivar BRSMG-Majestoso presented the highest grain yield (3,805 kg ha-1), and IPR-Andorinha and BRSMG-Madrepérola stood out with GYS≥12 of 93% and grains with clear integument, respectively. Grain yield increases as the number of pods per plant and grains per pod increase, presenting a negative correlation with crude protein contents and cooking time.

Inoculation with Rhizobium tropici can totally replace N-fertilization in the recently released BRSMG Uai bean cultivar

The BRSMG Uai common bean cultivar (Phaseolus vulgaris L.) unites traits required by producers, such as high yield and upright stands, the latter of which is considerably improved in relation to previously released cultivars such as BRSMG Madrepérola and Pérola. However, the potential of the cultivar to form symbiotic relationships with rhizobia to fix N2 has not yet been assessed. Here, field experiments were conducted to assess the efficiency of biological nitrogen fixation (BNF) in the cultivar, and to compare this to the efficiencies of other genetic materials widely grown throughout Brazil (BRSMG Madrepérola and Pérola). Experiments were conducted on two Oxisols in Minas Gerais State, Brazil (one during the dry season in the south, and the other during the winter season in the Alto-Paranaíba region), under a no-tillage system over maize stover. A randomized block experimental design was implemented in a 3 × 3 factorial arrangement with four replicates. Individual and combined Analysis of Variance were performed and the data were normalized. Homogeneity of the residual mean squares was observed. Significant effects of factors were identified by Scott-Knott tests and F tests. The BRSMG Uai cultivar responded to inoculation with the rhizobial strain CIAT 899, with yields similar to those achieved upon fertilization with 80 kg ha-1 of N-urea and to the yields of the BRSMG Madrepérola and Pérola cultivars inoculated with the same rhizobial strain. In terms of commercial use, this cultivar may be successful under either of the management practices adopted for N supply, and from the perspective of plant breeding, it has potential for inclusion in breeding programs directed toward improving BNF.