The primary argument for including large numbers of known-to-be innocent fillers in lineups is that guessing-based selections are dispersed among a large number of lineup members, leading to low innocent-suspect identification rates. However, a recent study using the two-high threshold eyewitness identification model has demonstrated advantages of smaller lineups at the level of the processes underlying the observable responses. Participants were more likely to detect the presence of the culprit and less likely to select lineup members based on guessing in smaller than in larger lineups. Nonetheless, at the level of observable responses, the rate of innocent-suspect identifications was higher in smaller compared to larger lineups due to the decreased dispersion of guessing-based selections among the lineup members. To address this issue, we combined smaller lineups with lineup instructions insinuating that the culprit was unlikely to be in the lineup. The goal was to achieve a particularly low rate of guessing-based selections. These lineups were compared to larger lineups with neutral instructions. In two experiments, culprit-presence detection occurred with a higher probability in smaller compared to larger lineups. Furthermore, instructions insinuating that the culprit was unlikely to be in the lineup reduced guessing-based selection compared to neutral instructions. At the level of observable responses, the innocent-suspect identification rate did not differ between smaller lineups with low-culprit-probability instructions and larger lineups with neutral instructions. The rate of culprit identifications was higher in smaller lineups with low-culprit-probability instructions than in larger lineups with neutral instructions.