Cueva De Research Articles

Enrichment of acid-tolerant sulfide-producing microbes from an acidic pit lake.

High concentrations of harmful metal(loid)s and extreme acidity are persistent environmental concerns in acidic pit lakes. In this study, we examine Cueva de la Mora (CM), a meromictic pit lake in the Iberian Pyrite Belt, Spain, as a model system. Our research aims to explore potential bioremediation strategies to mitigate the impacts of metal(loid)s and acidity in such environments. The major strategy applied in this research is to biologically stimulate sulfate reduction (i.e., biosulfidogenesis) in the deep layer of the lake to promote the formation of low-solubility sulfide minerals. Previous omics-based studies of CM have shown that several sulfate-reducing bacteria (SRB) taxa are present in the deep layer. However, their activities are likely limited by the availability of electron donors for sulfide production. Therefore, different amendments (glycerol, elemental sulfur, and glycerol + elemental sulfur) were tested to promote sulfide production and enrich acid-tolerant sulfide-producing microbes. Our results showed that glycerol stimulated dissimilatory sulfate reduction much faster than elemental sulfur alone, suggesting that electron donor limitations control sulfide production. Furthermore, the combined addition of glycerol and elemental sulfur (S(0)) resulted in the highest level of sulfide production. This indicates that S(0) can play a significant role as an electron acceptor in further promoting sulfide production when a suitable electron donor is present. Microbial community analysis revealed that Desulfosporosinus acididurans, a previously discovered acid-tolerant SRB, was enriched and became the dominant species in incubations with glycerol only (~76-96% abundance) or the combination of glycerol and S(0) (~93-99% abundance).

The Role of Sustainability in the Implementation of Tourism Projects as a Conservation Strategy of the Sierra Patlachique ANP, Case Cuevas de la amistad

The Role of Sustainability in the Implementation of Tourism Projects as a Conservation Strategy of the Sierra Patlachique ANP, Case Cuevas de la amistad

Shepherding the past: High-resolution data on Neolithic Southern Iberian livestock management at Cueva de El Toro (Antequera, Málaga).

The feeding strategies of the first domesticated herds had to manage the risks arising from the novelty of livestock practices in territories often distant from the animals' primary habitats. The Iberian Peninsula is characterised by a great diversity of environments, which undoubtedly influenced these dynamics. At the beginning of the Neolithic period these led the possibility to combine diverse livestock farming practices based on different animal feeding habits. This variability is also consistent with the rythms of adoption of domesticated animals, being later on the northern area. In order to address this issue, this work focuses on the dietary regimes of early sheep herds from southern Iberia, an area for which information is currently scarce. This study utilises high-resolution radiocarbon dating and stable isotope data on teeth to investigate sheep husbandry management strategies in Cueva de El Toro (Antequera, Málaga). The radiocarbon dates on the analysed remains evidenced they were deposited at the site over a short period, supporting the recurrent use of the cave. The sequential analysis of oxygen and carbon isotopes in tooth enamel reveals distinct livestock management strategies, reproduction patterns, feeding habits, and mobility during this short period. This variability demonstrates that livestock management practices in the western Mediterranean are more diverse than previously considered. Furthermore, these findings support the hypothesis that early Neolithic communities in the southern Iberian Peninsula were able to adopt different feeding strategies within the same herd, depending on their ecological and productive needs.

The nature and distribution of putative non-functional alleles suggest only two independent events at the origins of Astyanax mexicanus cavefish populations

BackgroundSeveral studies suggested that cavefish populations of Astyanax mexicanus settled during the Late Pleistocene. This implies that the cavefish’s most conspicuous phenotypic changes, blindness and depigmentation, and more cryptic characters important for cave life, evolved rapidly.ResultsUsing the published genomes of 47 Astyanax cavefish from la Cueva de El Pachón, El Sótano de la Tinaja, La Cueva Chica and El Sótano de Molino, we searched for putative loss-of-function mutations in previously defined sets of genes, i.e., vision, circadian clock and pigmentation genes. Putative non-functional alleles for four vision genes were identified. Then, we searched genome-wide for putative non-functional alleles in these four cave populations. Among 512 genes with segregating putative non-functional alleles in cavefish that are absent in surface fish, we found an enrichment in visual perception genes. Among cavefish populations, different levels of shared putative non-functional alleles were found. Using a subset of 12 genes for which putative loss-of-function mutations were found, we extend the analysis of shared pseudogenes to 11 cave populations. Using a subset of six genes for which putative loss-of-function mutations were found in the El Sótano del Toro population, where extensive hybridization with surface fish occurs, we found a correlation between the level of eye regression and the amount of putative non-functional alleles.ConclusionsWe confirm that very few putative non-functional alleles are present in a large set of vision genes, in accordance with the recent origin of Astyanax mexicanus cavefish. Furthermore, the genome-wide analysis indicates an enrichment of putative loss-of-function alleles in genes with vision-related GO-terms, suggesting that visual perception may be the function chiefly impacted by gene losses related to the shift from a surface to a cave environment.The geographic distribution of putative loss-of-function alleles newly suggests that cave populations from Sierra de Guatemala and Sierra de El Abra share a common origin, albeit followed by independent evolution for a long period. It also supports that populations from the Micos area have an independent origin. In El Sótano del Toro, the troglomorphic phenotype is maintained despite massive introgression of the surface genome.

Presence of the Solutrean on the North Eastern side of the Pyrenees: The case of Ruisseau de la Boulière 2

Until recently, the extreme Eastern part of the Northern Pyrenean Piedmont was a "vacuum" on the distribution map of the Solutrean. As a matter of fact, only two sites, the Embullas cave and Espassoles open-air site, reported the Solutrean on the Pyrénées-Orientales territory which covers an area of over 4000 square kilometers.
 There is no explanation to justify this situation, since this zone is flanked by two important Solutrean areas. Indeed, further South, just beyond the Pyrenees there are a number of Solutrean sites in Spanish Catalonia: l’Arbreda and Reclau Viver caves in the Serinya region. For hundred and forty kilometers towards the North East, a set of remarkable Solutrean sites spread over Eastern Languedoc along the right bank of the Rhône: the Oullins, Chabot, Pâques and La Salpétrière caves in the Gard and Ardèche regions. Along the Mediterranean coast, between these two clusters, the Solutrean presence is more discreet, in the form of a discontinuous structure of sites (la Roque cave, the Col de Gigean site and the caves of Bize and La Crouzade).
 The publication in 2014 of the open-air site of the Vigne Bertrand at Vingrau (Pyrénées Orientales, France), close to the Espassoles site, revived the interest for the Corbières zone, a low mountainous area near the Pyrenees.
 The Ruisseau de la Boulière 2, located very close to the first two sites, confirms the Solutrean presence in this micro-zone. The Eastern Corbières appear today as an identifiable landmark of the Solutrean before crossing the Pyrenees. On the other hand, the discovery of a Montaut type bifacial asymmetrical point in the site leads us to suggest the hypothesis of east-western mobility, along the northern and/or southern Pyrenean piedmonts. The existence of such connections has already been reported on the basis of techno-typological convergences between Aragón (Cueva de Chaves) and Hérault (La Salpétrière) lithic industries.

Multiple compression directions and basinal response in a rhomb-shaped terrestrial basin: The Cuevas de Cañart syncline (Iberian Chain, eastern Spain)

Rhomb-shaped basins have been commonly attributed to strike-slip tectonics, linked to fault bends and jogs (pull-apart basins). The case-study presented in this work (Las Cuevas de Cañart basin, Iberian Chain) is a 2 km × 5 km continental basin that shows a particular combination of geometric and sedimentary features. It shows a syncline geometry with steeply-dipping limbs, thickness of syn-tectonic sedimentary continental filling of about 1000 m, high amplitude/wavelength ratio, and different fold trends, some of them oblique to the main trend of the Iberian Chain. All these features make it an interesting case of a mixed-type basin, sharing both compressional and strike-slip features. The Cuevas de Cañart basin shows a Mesozoic, pre-compressional sequence linked to the extensional processes dominating in the marginal zones of the Maestrazgo basin, with a series up to 1500 m thick of limestones, sandstones and shales. These units overlie a detachment level (Upper Triassic Keuper facies) and show thickness variations controlled by NW-SE and NE-SW striking normal faults. During the Cenozoic compression, both fault systems were re-activated, and newly formed E-W folds (perpendicular to one of the dominant shortening directions) also appeared in the southern basin margin. The detailed structural study of this basin evidences the role of different types of structures (basement faults, thrusts formed under different compression directions) and the relevance of the detachment level in the evolution of compressional basins and thin-skinned fold-and-thrust belts.

The earliest basketry in southern Europe: Hunter-gatherer and farmer plant-based technology in Cueva de los Murciélagos (Albuñol).

Plant material culture can offer unique insights into the ways of life of prehistoric societies; however, its perishable nature has prevented a thorough understanding of its diverse and complex uses. Sites with exceptional preservation of organic materials provide a unique opportunity for further research. The burial site of Cueva de los Murciélagos in southern Iberia, uncovered during 19th-century mining activities, contained the best-preserved hunter-gatherer basketry in southern Europe, together with other unique organic artifacts associated with the first farming communities, such as sandals and a wooden hammer. We present 14 14C dates for the perishable artifacts (N = 76), situating the assemblage between the Early and Middle Holocene (c. 7500 to 4200 cal BCE). Our integrated analysis includes raw material determination and technological and chrono-cultural contextualization of this unique and important set of materials.

As above, so below: Deposition, modification, and reutilization of human remains at Marmoles cave (Cueva de los Marmoles: Southern Spain, 4000-1000 cal. BCE).

The deposition and manipulation of human remains in natural caves are well known for the Neolithic of Southern Iberia. The cultural meaning of these practices is however still largely unclear. Cueva de los Marmoles (CM, Priego-Córdoba) is one of the most important cave contexts from Southern Spain, which returned a large number of commingled skeletal remains suggesting its funerary use from the Neolithic to the Late Bronze Age. Here we discuss CM from a chronological and cultural perspective based on new radiocarbon, anthropological, and taphonomic analyses. These include the estimation of the minimum number of individuals, the exploration of fragmentation patterns characterizing different skeletal regions, and the macroscopic and microscopic analysis of modifications to the remains of possible anthropic origin. Radiocarbon data point to a funerary use of CM between the 5th -2nd millennium cal. BCE. MNI estimates reveal the presence of at least 12 individuals (seven adults and five nonadults). The low representation of elements from hands and feet suggests that individuals were placed in the cave while partially decomposed. Anthropic traces on the remains (e.g. fresh fractures, marrow canal modifications, and scraping marks) hint at their intentional fragmentation, cleaning from residual soft tissues, and in some cases reutilization. These practices are well-exemplified by the recovery of one "skull cup" and of two long bones used as tools. These data align with those from other cave contexts from the same geographic region, suggesting the presence, especially during the Neolithic period, of shared ideologies centered on the human body.

Study of Micro-Samples from the Open-Air Rock Art Site of Cueva de la Vieja (Alpera, Albacete, Spain) for Assessing the Performance of a Desalination Treatment.

In this work, some micro-samples belonging to the open-air rock art site of Cueva de la Vieja (Alpera, Albacete, Spain) were analysed. These samples were collected after and before a desalination treatment was carried out, with the aim of removing a whitish layer of concretion that affected the painted panel. The diagnostic study was performed to study the conservation state of the panel, and to then confirm the effectiveness of the treatment. Micro energy dispersive X-ray fluorescence spectrometry, Raman spectroscopy, and X-ray diffraction were employed for the characterization of the degradation product as well as that of the mineral substrate and pigments. The micro-samples analysis demonstrated that the painted layer was settled on a dolomitic limestone with silicon aggregates and aluminosilicates as well as iron oxides. The whitish crust was composed by sulfate compounds such as gypsum (CaSO4·2H2O) with a minor amount of epsomite (MgSO4·7H2O). An extensive phenomenon of biological activity has been demonstrated since then in almost all of the samples that have been analysed, and the presence of calcium oxalates monohydrate (CaC2O4·H2O) and dehydrate (CaC2O4·2H2O) were found. The presence of both calcium oxalates probably favoured the conservation of the pictographs. In addition, some carotenoids pigments, scytonemin (C36H20N2O4), and astaxanthin (C40H52O4) were characterized both by Raman spectroscopy and by X-ray diffraction. Hematite was found as a pigment voluntarily used for the painting of the panels used in a mixture with hydroxyapatite and amorphous carbon. The results of the analyses of the samples taken after the cleaning treatment confirmed a substantial decrease in sulphate formation on the panel surface.

Mesolithic human remains at Cueva de la Cocina: Insights from bioarchaeology and geochemistry

A series of previously unpublished human remains were found at Cueva de la Cocina (Dos Aguas, Valencia, Spain) in excavations from the 1940s and 1970s. Our analyses indicate a minimum of 5 individuals were present in the archaeological deposit, including both adults and children. AMS radiocarbon dating and osteological analysis document ritual defleshing or skinning during the Late Mesolithic, while δ13C and δ15N stable isotope analysis and comparisons to contemporary Mesolithic human remains from the region suggest that these individuals were part of a broader subsistence and cultural network of Holocene hunter-gatherers. Ancient DNA data indicate these late Mesolithic individuals were descendants of a major spread of a new population after a population turnover at the end of the Late Glacial. This research highlights the importance of long-term curation of archaeological finds and revisiting legacy collections.

A multi-stage Bayesian modelling for building the chronocultural sequence of the Late Mesolithic at Cueva de la Cocina (Valencia, Eastern Iberia)

This paper presents a refined Mesolithic chronocultural sequence as a result of matching data provided by the set of archaeological research conducted at Cueva de la Cocina (Valencia, Spain) in the 20th and 21st centuries and the new radiocarbon dates record. Because available data are of different quality, we apply a methodological framework based on Bayesian modelling approaches. To do this, we systematically order each one of the archaeological registers and then combine the information in a unitary general chronology. Our novel approach introduces Bayesian modelling from a double analytical procedure: using Bayesian chronological models applied to the stratigraphic sequence of Pericot's excavation in Cocina cave we build a general phase model using data from multiple years of archaeological fieldwork. One the most reliable layers have been defined, we use this information to define the rest of the sequence through a Predictive Bayesian approach. This approach sheds light on evolutionary questions from a macroscale regarding the socioecological dynamics of the last hunter-gatherers and their role for explaining the subsequent agricultural spread.

First record of a freshwater cave sponge (Porifera, unknown gen. and sp.) in a cave inhabited by Astyanax cavefish in the Sierra de El Abra, San Luis Potosí, Mexico

The karstic cave, la Cueva de Los Sabinos, located in the Sierra de El Abra in the state of San Luis Potosí, Mexico, is mostly known for hosting a population of blind, depigmented Astyanax mexicanus cavefish. Herein, we report the discovery of a non-pigmented sponge (Porifera) in the final sump of this cave. No genus or species name could be attributed because we did not collect any specimen. Up to now, the sponge distribution seems restricted to a single pool in la Cueva de Los Sabinos, but further careful exploration of other pools of the cave as well as closely related cavities is warranted. To our knowledge, this observation constitutes the fourth report of a freshwater, white, cave-adapted sponge in the world and the first for Mexico and North America. It is also the eleventh troglobite species encountered in Los Sabinos. Our discovery confirms the exceptionally rich biodiversity of this cave ecosystem.

THE “CUEVAS DE LOS MOROS” IN BOCAIRENTE (ES). ON THE INTEGRATED EXPEDITIOUS SURVEY

Abstract. The work supports the research on rocky settlements in the Mediterranean area and their diffusion. In particular, it provides the Valencian Community and the Municipality of Bocairente with the two-three-dimensional graphic documentation of the monument "Cuevas de Los Moros", apparently poor, which is the driving factor of the cultural economy of the country. The documentation created is a useful interdisciplinary tool for in-depth analysis of the place through historical, archaeological, and economic studies. The work has scientific rigor due to the methodically tackling data acquisition. It integrates the traditional instrumental survey, double-decametre, and level with photographic documentation and SFM processing. Furthermore, one of the objectives is to disseminate a correct methodological approach to documenting cultural heritage. The aim is to share and allocate among culturally motivated amateur operators to increase the documentation of a cultural heritage, which due to its widespread extension and apparent poverty, does not receive the necessary attention for promotion and conservation by the bodies responsible for the control of cultural heritage. Rock dwelling and its environmental system, as well as its rock-cut monuments, are recognized by Unesco.

Refining chronologies and typologies: Cueva de la Cocina (Dos Aguas, Valencia, Spain) and its central role in defining the Late Mesolithic sequence in the Iberian Mediterranean area

This paper constitutes a narrative view of the diachronic research conducted at Cueva de la Cocina (Dos Aguas, València) and its consequences on the Spanish Mesolithic literature from the middle of the 20th century to current times. Since its discover in 1941, the site has become one of the key sites regarding the sequence of the Late Mesolithic in Mediterranean Iberia. The campaigns conducted by L. Pericot (1941-45) provided the first archaeological sequence between the end of the Palaeolithic and the Early Neolithic in the aforementioned area. The review developed by J. Fortea in his fundamental book, focused on the Mediterranean “Epipalaeolithic”, reinforced the initial postulates on the occupations of the last hunter-gatherers and gave place to the current sequence. The goal of this work consists of providing some light in the epistemological trajectory that conforms the Mesolithic's research history. Particularly, we want to highlight the importance to understand pioneering works to open new research questions considering current archaeological challenges and chances.

Molluscs as Personal Adornment in a Gravettian Context from Cueva de Ardales (Málaga, Spain)

ABSTRACT Molluscs used as personal adornment in Gravettian context are scarce in the Iberian Peninsula. Less than 200 pieces have been barely found along the Mediterranean, and most of them in coastal sites. This article presents thirteen new items – in the majority of marine origin – found by a Spanish-German team during a joint research project in Cueva de Ardales (Ardales, Malaga, Spain). The cave is one of the few interior sites in which this type of finds has been attested and therefore shows the connections of the coast with the hinterland (away from the current Mediterranean coastline about 50 km currently and 60–65 kms. around 30 ka). The article also presents archaeozoological and archaeometric analysis of the molluscs, especially Raman spectrometry and X-ray diffraction.

Palpigrades from Cuba (Arachnida: Palpigradi: Eukoeneniidae).

The knowledge of palpigrades in Cuba is limited to the species Eukoenenia orghidani, discovered and described from Cueva de Bellamar. In this work, a survey for palpigrades in the suburbs of La Habana, Cuba revealed three species, Eukoenenia berlesei, Eukoenenia florenciae and a new species described here as Eukoenenia glandulosa sp. nov. Interestingly, the three species coexist in the microspaces of wet soil. A total of 16 arthropod species were identified living in the same microhabitat as the palpigrades.

The geometric microliths of cueva de la cocina and their significance in the mesolithic of Eastern Iberia: A morphometric study

This work presents the analysis of the geometric microliths of Cueva de la Cocina (València, Spain). It does so considering the hypothesis that, given the size of the cave's assemblage and its distinctive cultural markers, it could have acted as an aggregation site for the Late Mesolithic in the Mediterranean Iberia. The work presented here uses the cultural similarity of the geometric microliths of different sites to test this hypothesis, relying on several methodological techniques such as tactical simulation and geometric morphometrics, while offering proposals for the correction of data imbalance in archaeological data. As a conclusion, our data points to the fact that, for the last phase of the Late Mesolithic (phase B in the regional sequence) the site could have indeed acted as an aggregation site, but we require caution on the results of our analysis fundamentally on two grounds: (1) whether the cultural variation of the geometric microliths offers enough resolution for microscale analysis and (2) the necessity to combine this proxy with other types of archaeological artefacts which could give (or rest) strength to our hypothesis.

The Preclassic military macaw (Ara militaris) from the Cueva de Avendaños (Chihuahua, Mexico) was not locally procured

AbstractThe oldest macaw specimen in Pre‐Hispanic Mexico and the southwestern United States was recovered in Cueva de Avendaños, Chihuahua, Mexico, at the base of the Sierra Madre Occidental. It dates to the Late Archaic/Early Agricultural period (1929–2057 cal BP). The head is the only preserved element of the macaw and it was mummified through natural causes. Research on this specimen examines its sex identification and its geographical range, questioning whether this specimen belongs to a local population living in the vicinity of the cave. A species distribution model over the last 2000 years was elaborated to ascertain the presence of military macaw in the study area. Our results show that the macaw was non‐local, sustaining the idea that the practice of early animal husbandry existed in the early stages of the Pre‐Hispanic world, and also suggest that a significant and rapid climate changes characterized the Holocene, affecting the military macaw's distributional range.

Use of cinnabar in funerary practices in the Central Pyrenees. Analysis of pigments on bones from the prehistoric burial of the Cueva de la Sierra cave in Campodarbe (Huesca, Spain)

The appearance of red pigments in prehistoric burial sites is frequent. In the study presented herein, the composition and provenance of the pigment sprinkled in a burial dated between the Late Neolithic and Early Chalcolithic periods in the Cueva de la Sierra, in Campodarbe (Huesca, Spain), were characterized through X-ray fluorescence spectroscopy, scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy, Raman spectroscopy, X-ray powder diffraction, and sulfur (δ34S) isotope analysis. The chemical composition of the pigment used in the burial could be unequivocally established as cinnabar, and the S isotope signature pointed to an origin in the ores of the Northwestern Iberian Peninsula, ca. 500 km far from the burial site, suggesting an alternative provenance to the Almadén outcrop, the main cinnabar source in the Iberian Peninsula. The presence of cinnabar in the Ebro valley, on the southern slope of the Central Pyrenees, forces us to rethink the complex processes of interaction between communities that populated the north of the Iberian Peninsula during the final moments of the 4th millennium BC.

The Acoustics of Aggregation Sites: Listening to the Rock Art Landscape of Cuevas de la Araña (Spain)

ABSTRACT Since the 1980s, research into aggregation sites has focused on the material dimension of the archaeological record and has thus led to an incomplete view of the prehistoric reality. Early communities chose sites for many reasons. In this article, we explore the possibility that something as immaterial in nature as the acoustics of the sites may have influenced the selective choices made by the prehistoric peoples who created and used rock art in these meeting places. Employing the Impulse Response (IR) methodology, we systematically analyze the acoustic properties of Cuevas de la Araña, an aggregation site in eastern Spain with Levantine rock art, and 16 satellite sites in its surroundings. The results indicate that the acoustics of La Araña could have been important for creating affective engagement through musical performances carried out during the production and use of rock art, as well as for the choice of the site as a meeting place for Levantine populations.