Ten species of chaetognaths were identified from plankton collections taken from the inner continental shelf waters off Virginia in 1960, including:Sagitta serratodentata, S. elegans, S. tenuis, S. enflata, S. helenae, S. minima, S. hispida, S. bipunctata, Pterosagitta draco, andKrohnitta pacifica. An artificial key to the identification of these species and descriptions based on the collected specimens are given. Tooth counts and seminal vesicle structure ofS. serratodentata revealed the presence of two subspecies in the western Atlantic. Virginian specimens are the cold-water vaviety,S. s. tasmanica, whereas Cuban waters are inhabited byS. s. atlantica. Published counts and descriptions of this species are lacking for intermediate waters. Specimens ofS. tenuis displayed an intermediacy and overlapping of several characters that have been used for the separation ofS. friderici andS. tenuis. Included among these characters are Tokioka’s TC values, maximum body length, number of teeth, extension of the ovaries, and size at maturity. The present collections ofK. pacifica constitute a northward extension of the known range of this species in the western Atlantic.