Cu In Roots Research Articles

Combined application of selected heavy metals and EDTA reduced the growth of Petunia hybrida L.

Up till now, despite of well-developed ornamental market, very little information is available on Petunia hybrida L. tolerance against heavy metals (HMs), which can contribute in both beautification of urban dwellings, as well as potential in phytoremediation. Therefore, hydroponic study was conducted to check the effects of Cd, Cr, Cu, Ni and Pb individually (50 and 100 μM) and with co-application of EDTA (2.5 mM) in Hoagland’s nutrient solution. Results indicated higher uptake of Cd, Cr, Ni and Pb in above ground parts, and Cu in roots, further the co-application of EDTA enhanced HMs uptake in P. hybrida L. This uptake accompanied changes in biochemical stress indicators, included significantly higher MDA, H2O2 contents and electrolyte leakage with reduced chlorophyll a, chlorophyll b, total chlorophyll and carotenoid content. Upon exposure to HMs increased antioxidant enzyme activities (CAT, POX, GST, APX, and SOD) were noted. Though selected HMs can be removed by using P. hybrida L., the findings of current study indicated that the direct exposure of P. hybrida L. to Cd, Cr, Cu, Ni and Pb damaged the plant’s aesthetics, and to use P. hybrida L. for beautification of urban landscape or phytoremediation, appropriate soil modification should be included.

Morpho-physiological traits, antioxidant capacity and phytoextraction of copper by ramie (Boehmeria nivea L.) grown as fodder in copper-contaminated soil.

Ramie (Boehmeria nivea L.), the oldest fiber crop in China, can also be grown as fodder crop because of its huge biomass production. Moreover, it has the potential to colonize heavy metal-contaminated soils which showed the possibilities of phytoremediation using B. nivea. Therefore, the present study was conducted to investigate the potential of B. nivea for phytoextraction of copper (Cu)-contaminated soil. Moreover, the impact of different concentrations of Cu on growth and antioxidant enzymatic activity by B. nivea were also studied. For this purpose, a pot experiment was conducted to examine the growth, antioxidative response, and localization (distribution) of Cu in B. nivea plant under different Cu concentrations (0, 50, 100, 200, 300, and 400mgkg-1 soil). Results revealed that B. nivea tolerated up to 100mgkg-1 Cu concentration without a significant decrease in biomass, but further increase in Cu concentration from 200 to 400mgkg-1 exhibited a significant reduction in chlorophyll content, fresh and dry biomass, plant height, and number of leaves. It was further observed that B. nivea accumulated more Cu in roots (26 to 53mgkg-1), followed by the leaves (23 to 28mgkg-1) and stems (14 to 21mgkg-1), while the values for both bioaccumulation factor (BAF) and translocation factor (TF) at all treatments were less than 1. Moreover, activities of antioxidative enzymes (superoxide dismutase and peroxidase) were initially increased with the exposure of 50, 100, and 200mgkg-1 Cu, but decreased by further increasing the Cu concentration to 300 and 400mgkg-1 indicating the oxidative stress which is manifested by high malondialdehyde (MDA) and proline contents also. Thus, based on results, it can be concluded that B. nivea accumulated relatively low Cu contents in aboveground parts and could be grown as fodder crop for phytoremediation of Cu-contaminated sites.

Screening of native tropical trees for phytoremediation in copper-polluted soils

Due to the limited number of studies on phytoremediation using native tree species in tropical soils, the aim was to identify new phytoremediator species from tropical climate with the purpose of promoting an increase in the diversity of tropical native trees used in phytoremediation projects. Seven native tree species from Brazil were selected: Cedrela fissilis, Handroanthus serratifolius, Copaifera langsdorffii, Hymenaea courbaril, Mimosa caesalpiniifolia, Cecropia sp. and Myracrodruon urundeuva. Seedlings of these species were planted in pots with an unpolluted Arenosol, and then spiked with 60, 100 and 500 mg kg−1 Cu. Height and stem diameters were measured over 60 days. Biomass and total Cu concentration were determined in leaves, stem and roots. Copper in bulk soils and rhizospheres was analyzed by a sequential extraction method. All species accumulated high concentration of Cu in roots (>300 mg kg−1), so they could be used as phytostabilizators for this metal. Copper mobilization increased in the rhizospheres, but it was mostly absorbed by roots. Cecropia sp., M. urundeuva and C. langsdorffii are hyperaccumulators of Cu (>300 mg kg−1 in shoots), so they are potential phytoextractor species. This study evidence the potential of seven tree species native from tropical regions for phytostabilizing copper-polluted soils.

Organic acid profile and phenolic and sugar content in Salix purpurea × viminalis L. cultivated with different spent mushroom substrate and copper additions

ABSTRACTThe aim of the study was to determine the response of Salix purpurea × viminalis L. growing on Pleurotus ostreatus spent mushroom substrate (SMS) with different copper (Cu) addition. The content of Cu in roots, leaves and shoots was reduced by SMS addition. A decrease of biomass was observed with simultaneous Cu concentration increase. SMS induced leaf and root elongation of Cu-treated plants. Variation in the profile of low molecular weight organic acids with the domination of oxalic and acetic acids was observed. The total phenolic content significantly increased for plants cultivated with SMS, while the biosynthesis of salicylic acid was considerably weakened. The content of sugars was generally reduced by SMS. Alteration in the level of the stress-related molecules suggests mitigation of the harmful effect of Cu on Salix hybrid metabolism by SMS addition. This pointed to the possibility of using SMS in contaminated soil to reduce the toxic effect of metals on plants used in phytoextraction.

Temporal Evolution of Copper Distribution and Speciation in Roots of Triticum aestivum Exposed to CuO, Cu(OH)2, and CuS Nanoparticles

Utilization of nanoparticles (NP) in agriculture as fertilizers or pesticides requires an understanding of the NP properties influencing their interactions with plant roots. To evaluate the influence of the solubility of Cu-based NP on Cu uptake and NP association with plant roots, wheat seedlings were hydroponically exposed to 1 mg/L of Cu NPs with different solubilities [CuO, CuS, and Cu(OH)2] for 1 h then transferred to a Cu-free medium for 48 h. Fresh, hydrated roots were analyzed using micro X-ray fluorescence (μ-XRF) and imaging fluorescence X-ray absorption near edge spectroscopy (XANES imaging) to provide laterally resolved distribution and speciation of Cu in roots. Higher solubility Cu(OH)2 NPs provided more uptake of Cu after 1 h of exposure, but the lower solubility materials (CuO and CuS) were more persistent on the roots and continued to deliver Cu to plant leaves over the 48 h depuration period. These results demonstrate that NPs, by associating to the roots, have the potential to play a role in slowly providing micronutrients to plants. Thus, tuning the solubility of NPs may provide a long-term slow delivery of micronutrients to plants and provide important information for understanding mechanisms responsible for plant uptake, transformation, and translocation of NPs.

Piriformospora indica, an excellent system for heavy metal sequestration and amelioration of oxidative stress and DNA damage in Cassia angustifolia Vahl under copper stress

Present investigation reveals copper induced phytotoxicity, oxidative stress and DNA damage in Cassia angustifolia Vahl and its amelioration by employing a symbiotic fungus, Piriformospora indica. Seeds were germinated on Knop's medium containing five Cu levels (0, 1, 10, 50, 100 and 200 mg L−1), with and without P. indica. Colonization with P. indica significantly (P < 0.05) ameliorated Cu induced oxidative stress. However, maximum amelioration was observed at 50 mg L−1 Cu with P. indica. Atomic absorption spectroscopy revealed that P. indica colonization significantly inhibited Cu accumulation in shoots. Maximum decline in Cu accumulation in shoots was observed at 50 mg L−1 (27.27%) with P. indica over Cu alone. Besides, P. indica colonized seedlings stored 16.86% higher Cu in roots as compared to Cu alone at 200 mg L−1. Similarly, maximum proline accumulation increased up to 19.32% over Cu alone at 50 mg L−1 Cu with P. indica. Significant elevation in antioxidant enzyme levels of superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase and glutathione reductase was seen with P. indica. Contrary to increase in antioxidant level, toxic parameters such as lipid peroxidation and hydrogen peroxide decreased significantly with P. indica. Maximum decline in lipid peroxidation (13.76%) and hydrogen peroxide (18.58%) was observed at 50 mg L−1 with P. indica over Cu alone. P. indica significantly reduced DNA damage as well as changed the protein profile in C. angustifolia seedlings. Thus, P. indica proved to be an excellent system to alleviate Cu induced oxidative stress and might be useful as a phytostabilization tool.

Physiological and biochemical effects of nanoparticulate copper, bulk copper, copper chloride, and kinetin in kidney bean (Phaseolus vulgaris) plants

It is essential to understand the interactions of engineered nanoparticles (ENPs) with additives used in agriculture and their impacts on crop plants. In this study, kidney bean (Phaseolus vulgaris) plants were grown in potting soil amended with either nano copper (nCu), bulk copper (bCu), or copper chloride (CuCl2) at 0, 50, and 100mg/kg, combined with 0, 10, or 100μM of kinetin (KN). Plant growth, Cu, micro and macroelement concentrations, chlorophyll content, and enzymatic activity were examined in 55-day old plants. Results showed that root Cu content was at least 10-fold higher, compared to other tissues. Accumulation of Cu in roots was decreased by 100μM KN up to 25%. A concentration-dependent increase of Cu content in leaves by Cu×KN was observed. Chlorophyll production was diminished by CuCl2+KN between 22 and 30%, showing a hormetic response. Catalase activity was repressed by 65% to 82% in bCu and CuCl2 treatments. From all essential elements, Ca, Mn, and P were reduced by 33% to 97% in bCu, CuCl2, and CuCl2+KN treatments. However, this did not impact stem elongation and tissue biomass that increased up to 55% under exposure to bCu and CuCl2. Our results demonstrate that KN combined with ionic Cu could have negative implications in kidney bean plants, since this combination impacted chlorophyll production and nutrient element accumulation.

Phytoremediation of metals using lemongrass (Cymbopogon citratus (D.C.) Stapf.) grown under different levels of red mud in soil amended with biowastes

ABSTRACTDue to hostile condition of red mud (RM), its utilization for vegetation is restricted. Therefore, RM with biowastes as soil amendment may offer suitable combination to support plant growth with reduced risk of metal toxicity. To evaluate the effects of RM on soil properties, plant growth performance, and metal accumulation in lemongrass, a study was conducted using different RM concentrations (0, 5, 10, and 15% w/w) in soil amended with biowastes [cow dung manure (CD) or sewage-sludge (SS)]. Application of RM in soil with biowastes improved organic matter and nutrient contents and caused reduction in phytoavailable metal contents. Total plant biomass was increased under all treatments, maximally at 5% RM in soil with SS (91.4%) and CD (51.7%) compared to that in control (no RM and biowastes). Lemongrass acted as a potential metal-tolerant plant as its metal tolerance index is >100%. Based on translocation and bioconcentration factors, lemongrass acted as a potential phytostabilizer of Fe, Mn, and Cu in roots and was found efficient in translocation of Al, Zn, Cd, Pb, Cr, As, and Ni from roots to shoot. The study suggests that 5% RM with biowastes preferably SS may be used to enhance phytoremediation potential of lemongrass.

Spatial imaging and speciation of Cu in rice (Oryza sativa L.) roots using synchrotron-based X-ray microfluorescence and X-ray absorption spectroscopy

Knowledge of elemental localization and speciation in rice (Oryza sativa L.) roots is crucial for elucidating the mechanisms of Cu accumulation so as to facilitate the development of strategies to inhibit Cu accumulation in rice grain grown in contaminated soils. Using synchrotron-based X-ray microfluorescence and X-ray absorption spectroscopy, we investigated the distribution patterns and speciation of Cu in rice roots treated with 50 μM Cu for 7 days. A clear preferential localization of Cu in the meristematic zone was observed in root tips as compared with the elongation zone. Investigation of Cu in the root cross sections revealed that the intensity of Cu in the vascular bundles was more than 10-fold higher than that in the other scanned sites (epidermis and cortex) in rice roots. The dominant chemical form of Cu (79.1%) in rice roots was similar to that in the Cu-cell wall compounds. These results suggest that although Cu can be easily transported into the vascular tissues in rice roots, most of the metal absorbed by plants is retained in the roots owing to its high binding to the cell wall compounds, thus preventing metal translocation to the aerial parts of the plants.

Avaliação do potencial fitorremediador de crisântemo em solo com excesso de cobre

Minimizing the harmful effects of copper (Cu) in the soil, using plants are slow and gradual, requiring the identification of species with fitorremediativa fitness for this process. Thus, the present work had as objective to evaluate the cultivation of chrysanthemum cv. Dark Fiji in soil added with Cu as promising phytoremediation. The experiment was conducted in the period from July to December 2014, in the greenhouse of the Floriculture UFSM. In a completely randomized experimental design, with five treatments composed of doses of Cu added to the soil, in the amounts of 250, 500, 750 and 1,000 mg kg-1 and control (without addition), with five replications. In two crop cycles both with duration of 104 days from the production of seedlings to harvest. Chrysanthemum cuttings were obtained from cuttings collected in the garden clonal itself, with 8 cm long, rooted in commercial substrate and transplanted into containers containing soil. They evaluated phytotechnical parameters and translocation factors of aerial part of bioaccumulation and bioconcentration factor of Cu in plant roots and metal extraction rate. It was observed that at all doses of Cu added to the soil, the plants showed low plant development and floriferous affecting its aesthetic quality in both crop cycles. The high accumulation of Cu in the roots is indicative of growing tolerance, cv. Dark Fiji in areas with excess of this, with phytoremediation potential.

Assessment of Heavy Metals Contamination in Reclaimed Mine Soil and their Accumulation and Distribution in Eucalyptus Hybrid.

The metal contamination in reclaimed mine soil (RMS) of Jharia coal field, Dhanbad (India) using various contamination indices and their accumulation in tissues of Eucalyptus hybrid were assessed. In RMS, metal concentrations were found higher (202%-533%) than control soil (CS) with major contribution of Co and Mn followed by Zn, Cu and Pb. Principal component analysis (PCA) of metals present in RMS was carried out to assess their origin in RMS. The contamination factor (CF) values in RMS indicated moderate to very high level of pollution (ranged between 2.02 and 5.33). Higher accumulation of Pb in barks (three times), Zn in leaves (4.5 times), Mn in leaves (19 times), and Cu in roots (1.4 times) was found in trees growing on RMS than CS. The study concluded that different tree tissues accumulate varied concentration of heavy metals in RMS and thus for biomonitoring of metals, specific tissues has to be selected.

Copper-Induced Responses in Poplar Clones are Associated with Genotype- and Organ-Specific Changes in Peroxidase Activity and Proline, Polyamine, ABA, and IAA Levels

The involvement of auxin, abscisic acid (ABA), polyamines (PAs), and proline in adaptation to long-term exposure of woody plants to high levels of heavy metals in soil has received scant attention, even in poplar which is a good candidate for phytoremediation of metal-polluted soils and is regarded as a model for basic research in tree species. Three poplar clones (M1, PE19/66, and B229) were comparatively analyzed in a pot experiment for their responses to 300 mg kg−1 Cu(NO3)2 at morphological, physiological, and biochemical levels. After 4 months, despite the prevalent accumulation of Cu in roots, where the metal reached potentially toxic concentrations, the three clones showed distinct Cu accumulation and translocation capacities, whereas they did not display evident toxicity symptoms or growth inhibition. Several protective mechanisms, namely decreased photosynthetic functionality, enhanced guaiacol peroxidase (GPOD) activity, and accumulation of proline and PAs, were differentially activated in Cu-treated plants in an organ- and clone-specific manner. Overall, a positive relationship between root Cu concentration with GPOD, proline, and PAs was observed. In M1, higher Cu accumulation in roots and leaves compared with other clones was reflected in stimulation of GPOD activity in both organs and in enhanced proline, and PA levels. In PE19/66, these responses were observed only in roots concomitant with high Cu accumulation. Clone B229 accumulated very low amounts of Cu, therefore, these defense responses were attenuated compared with other clones. Enhanced ABA concentrations in response to Cu were observed in PE19/66 and B229; this was likely responsible for stomatal limitation of photosynthesis in PE19/66, whereas in B229 this effect may have been counteracted by increased IAA. Essentially unchanged leaf auxin levels under Cu stress may account for the lack of shoot growth inhibition observed in all three clones; B229 was the only clone that displayed Cu-induced IAA accumulation in roots. Results are discussed in terms of clone-specific adaptive mechanisms to Cu stress in tolerant poplars.

Copper translocation and tolerance in seedlings of tree species grown in contaminated soil

ABSTRACTHigh concentrations of copper (Cu) may be toxic to plants, yet some species can be tolerant to soils contaminated by this metal. The study aimed to evaluate Cu translocation and tolerance in seedlings of ‘timbaúva’ (Enterolobium contortisiliquum (Vell.) Morong), ‘pata-de-vaca’ (Bauhinia forficata Link) and ‘amendoim-bravo’ (Pterogyne nitensTul) subjected to Cu-contaminated soil. The study was conducted in a greenhouse for 120 days, using as substrate a clayey Oxisol. The experiment was set in a completely randomized design, using a 3 x 6 factorial scheme, corresponding to three forest species (‘timbaúva’, ‘pata-de-vaca’ and ‘amendoim-bravo’) and six Cu doses in the soil (0, 60, 120, 180, 240 and 300 mg kg-1), with six replicates. The following variables were analysed: total dry matter, Cu content and its accumulation in roots and shoots, tolerance and translocation indices and the coefficient of impact of the contamination on the relative contents of Cu in roots and shoots. ‘Timbaúva’ showed lower reduction in total dry matter with the increase of Cu doses in the soil, proving to be able to decrease Cu translocation to the shoots and tolerate doses of up to 236 mg kg-1 of this metal in the soil. ‘Timbaúva’ seedlings are more tolerant to contamination of Cu in soil, compared with seedlings of ‘amendoim-bravo’ and ‘pata-de-vaca’.

Effects of the Addition of Selenium on Trace Element Concentrations in Danshen (Salvia miltiorrhiza)

Danshen (Salvia miltiorrhiza), a Chinese herbal medicine, was cultivated under three conditions of selenium enrichment. Fifteen trace and minor elements were determined in different tissues of the plant by inductively coupled plasma-mass spectrometry and hydride generation-atomic fluorescence spectrometry after microwave digestion. The goals of this work were to investigate the concentrations of trace elements in Danshen and determine whether the element concentrations of As, Cd, Hg, Pb, and Cu exceeded the safety standards; to compare the concentrations of fifteen elements in roots, stems, and leaves of Danshen under the same selenium enrichment level; to compare the concentrations of fifteen elements in the same tissue of Danshen under different selenium enrichment levels; and to compare the translocation factors after selenium enrichment. The results showed that the concentrations of elements As, Cd, Hg, Pb, and Cu in roots were lower than the specified values. In general, under the same selenium enrichment conditions, the elemental concentrations in roots were lower than those in stems and leaves. Furthermore, most element concentrations in leaves were higher than those in stems. The concentrations of Pb, Cr, and Hg in roots decreased while other elemental concentrations increased after selenium enrichment. Cu, Pb, Cd, Cr, and Hg were primarily transferred to stems and leaves when cultivated under selenium enrichment.

Study of Absorption of Heavy Metals (Pb,Cu) in &lt;i&gt;Ph&lt;/i&gt;&lt;i&gt;ragmites au&lt;/i&gt;&lt;i&gt;stralis&lt;/i&gt; in Lingang New City

Analysis on the concentrations of Pb, Cu in the roots, stems, leaves of phragmites australis which grows on Lingang New City of Shanghai showed that both of Pb, Cu in roots were higher concentrations than that in stems and leaves; the absorption of Pb,Cu had obvious seasonal variation; in winter, the enrichment of Pb, Cu in phranmites autralis was occurred and the enrichment ability of Pb was higher than Cu,

Effect of copper toxicity on root morphology, ultrastructure, and copper accumulation in Moso bamboo (Phyllostachys pubescens).

A hydroponic culture experiment was conducted to study the effect of copper toxicity on root morphology, ultrastructure, and copper accumulation in Moso bamboo (Phyllostachys pubescens). Root ultrastructure of Moso bamboo was studied by transmission electron microscopy and scanning electron microscopy. Application of 200 μM Cu resulted in an accumulation of 810 mg kg(-1) dry weight and 91 mg kg(-1) dry weight Cu in roots and shoots, respectively. The majority of the plants did not survive the application of 400 μM Cu. Biomass production declined consistently with application of each additional increment of Cu. Root growth was more severely inhibited than shoot growth. Cu adversely affected the root morphology of the plants, however, root surface area and number of root tips increased slightly at low levels of Cu. Root cell ultrastructure and organelles changed significantly under Cu stress, in particular, cell walls, mitochondria, and xylem parenchyma were affected.

НАКОПЛЕНИЕ ТЯЖЕЛЫХ МЕТАЛЛОВ В ОРГАНАХ ЗЕМЛЯНИКИ САДОВОЙ В УСЛОВИЯХ ТЕХНОГЕННОГО ЗАГРЯЗНЕНИЯ

In case the large strawberry plantations and the plots of amateur gardeners are located in the areas, influenced by industrial centers, the risk of soil and air pollution with heavy metals is high, and the background values of Cu, Zn and Pb, for instance, can be 4-55 times increased. In plant there are physiological barriers between the root and the stem, the stem and the leaf, and the stem and reproductive organs, which prevent penetration of the toxicants into fruits. During 2006-2008 we evaluated the accumulation of Pb, Ni, Zn, Fe, and Cu in roots, rhizomes, petioles, leaves and fruits in four strawberry Fragaria ½ ananassa (Duchesne ex Weston) varieties, grown on the light gray forest soil under multielement anthropogenic pollution in Orlovskaya Province. The accumulation of each element was shown to be specific, depending on its physiological effect and chemical properties, and on the anthropogenic load. Significant differences among the varieties were also observed. In Rubinovii kulon, Mamochka, and Bylinnaya varieties the Pb content in leaves was higher than in the roots, probably due to extra foliar absorption from air. In Bogema variety the Pb accumulation gradually decreased from the roots to rhizomes, then to leaves and finally to fruits. Considerable Ni content in the roots was indicated only in Mamochka variety. In other tested varieties the Ni accumulation in fruits was significantly higher than in the underground organs. Zn and Fe distribution among strawberry plant organs was specified by the varietal genetic features. Zn was not accumulated in the vegetative organs of Rubinovii kulon and Mamochka plants. Conversely, the high accumulation of Zn was found in the roots of Bogema plants and in the rhizomes of Bylinnaya plants. In Rubinovii kulon, Mamochka and Bogema plants the Fe accumulation was mainly detected in the roots and in the berries, whereas in Bylinnaya plants this element was mainly concentrated in the rhizomes and petioles. The least significant difference for factor A (strawberry varieties), factor B (plant organs) and their combination (AB) makes for Pb 0.24, 0.27, and 0.53, respectively; for Ni 0.04, 0.05, and 0.10, respectively; for Zn 0.24, 0.27, and 0.53, respectively; for Fe 0.79, 0.89, and 1.77, respectively; for Cu 0.47, 0.52, and 1.05, respectively. An unequal accumulation of toxicants among the varieties, which were not bred with a view to low heavy metal penetration under soil and air pollution, shows that the ecological risk of the heavy metal accumulation in fruits could be decreased by purposeful choosing varieties for cultivation with respect to their physiological features.

Effects of particle sizes of rock phosphate on immobilizing heavy metals in lead zinc mine soils

Abstract Phosphate-induced immobilization is recognized as one of effective in situ remediation methods for heavy metal contaminated soils. Phosphate-based minerals that adsorb, chelate, or complex heavy metals in soil were greatly concerned as effective heavy metals immobilizing materials. Effects of particle sizes of rock phosphate on immobilizing heavy metals in Pb-Zn mine soils by a greenhouse experiment was conducted. Rock phosphate was added to a Pb-Zn mine soil with four different particle sizes, D97<101.43 µm (UP), D97<71.12 µm (P1), D97<36.83 µm (P2) and D97< 4.26 µm (P3) (the diameters of 97% of the particles were less than 4.26 µm.), and 2 rates (2.5% and 5%). Lolium prenne , L. were grown in the treated soils. Compared to the control, addition of rock phosphate (RP) decreased metal contents in both roots and shoots significantly. Pb contents in shoots decreased by 19.59%-37.80% by different particle sizes at the rate of 5%, reaching lowest level at lowest particle size P3. Zn contents in shoots decreased by 13.47% -13.75 %, Cu in roots was decreased by 18.46%-67.98% and in shoots by 16.82%-32.61%, and Cd in roots decreased by 31.03%-74.23%. The results indicated that, RP can reduce the phytoavailability of Pb, Zn, Cu and Cd in soil significantly by immobilization and the effects strengthened with the decrease of particle size and increasing the rate of addition.

Exposure studies of core–shell Fe/Fe3O4 and Cu/CuO NPs to lettuce (Lactuca sativa) plants: Are they a potential physiological and nutritional hazard?

Iron and copper nanomaterials are widely used in environmental remediation and agriculture. However, their effects on physiological parameters and nutritional quality of terrestrial plants such as lettuce (Lactuca sativa) are still unknown. In this research, 18-day-old hydroponically grown lettuce seedlings were treated for 15 days with core–shell nanoscale materials (Fe/Fe3O4, Cu/CuO) at 10 and 20mg/L, and FeSO4·7H2O and CuSO4·5H2O at 10mg/L. At harvest, Fe, Cu, micro and macronutrients were determined by ICP-OES. Also, we evaluated chlorophyll content, plant growth, and catalase (CAT) and ascorbate peroxidase (APX) activities. Our results showed that iron ions/NPs did not affect the physiological parameters with respect to water control. Conversely, Cu ions/NPs reduced water content, root length, and dry biomass of the lettuce plants. ICP-OES results showed that nano-Cu/CuO treatments produced significant accumulation of Cu in roots compared to the CuSO4·5H2O treatment. In roots, all Cu treatments increased CAT activity but decreased APX activity. In addition, relative to the control, nano-Cu/CuO altered the nutritional quality of lettuce, since the treated plants had significantly more Cu, Al and S but less Mn, P, Ca, and Mg.

Selenium Fertilization Alters the Chemical Composition and Antioxidant Constituents of Tomato (Solanum lycopersicon L.)

Although selenium (Se) is a known anticarcinogen, little is known regarding how Se affects other nutritional qualities in crops. Tomato ( Solanum lycopersicon ) was supplied with 0-50 μM selenate and analyzed for elemental composition and antioxidant compounds. When supplied at low doses (5 and 10 μM) via the roots, Se stimulated the synthesis of phenolic compounds in leaves and reduced the levels of Mo, Fe, Mn, and Cu in roots. At higher doses (25 and 50 μM Se) leaf glutathione levels were 3-5-fold enhanced. Supply of selenate via foliar spray (0, 2, or 20 mg Se plant(-1)) resulted in Se-biofortified tomato fruits, with Se levels low enough not to pose a health risk. The Se-biofortified fruits showed enhanced levels of the antioxidant flavonoids naringenin chalcone and kaempferol and a concomitant decrease of cinnamic acid derivatives. Thus, tomato fruits can be safely enriched with Se, and Se biofortification may enhance levels of other neutraceutical compounds.