Background: According to the World Health Organisation, Chlamydia trachomatis (Ct) is the most commonsexually transmitted bacterial agent worldwide.The aim of the present study was to determine the prevalence and risk factors for genital infection with Ctamong sexually active women (14-60 years of age), in Turin (northern Italy).Methods: A cross-sectional study was performed between January 2002 and December 2007 among sexuallyactive women in Turin. All women were tested for Ct infection using the Amplified Ct Assay (Gen-Probe) onan endocervical specimen. The prevalence was calculated as the proportion of Ct-positive women out of thetotal study population. Associations between Ct infection and risk factors (socio-demographic, behavioural,and clinical-gynaecological) were analyzed using the Chi-square test. The level of significance was set at0.05. Risk factors independently associated with Ct infection were determined using a multivariate logisticregression model. The statistical analyses were performed using SPSS (version 17.0).Results: The study included 25,289 women, of whom 1.8% were found to have Ct infection. The prevalenceof Ct infection increased significantly from 1.5% in 2002 to 2.2% in 2007 (χ2 =5.6; p-value <0.05). Theprevalence significantly increased by decreasing age (χ2 linear trend 213.7; p-value <0.001) and wassignificantly higher among non-Italian women compared to Italian women (3.3% vs. 1.6%, p-value <0.001).In the multivariate analysis, Ct infection was significantly associated with an adjusted odds ratio higher than2 for young age (14-24 years), being non-Italian, having had 2 or more lifetime sexual partners, and havinghad more than 1 partner in the previous six months.Conclusions: Our results showed an increase in Ct prevalence in Turin between 2002 and 2007. A significantassociation between Ct infection and young age, multiple sexual partners, and originating from EasternEurope was observed. To reduce the spread of the infection, women with at least one of the above mentionedrisk factors, should be encouraged to undergo a free-of-charge Ct testing.