CT Max Research Articles

Multiple biomarkers response of Astyanax lacustris (Teleostei: Characidae) exposed to manganese and temperature increase

The present study aimed to evaluate the toxicity of Mn (6.65 mg/L) at different exposure times (96 h, 7, 14, and 21 days) and evaluate its possible toxic effects on the fish Astyanax lacustris through multi-biomarkers and the maximum critical temperature (CT Max). The results show an increase in the Mn accumulation (liver and gills) with increasing exposure time. The glutathione S-transferase (GST) activity showed differences in the group exposed to Mn for 96 h compared to the group exposed for 21 days. The acetylcholinesterase (AChE) activity increased in the fish exposed for 7 days compared to the control group. On the other hand, no genotoxic changes were observed. The CT Max showed that the loss of equilibrium of 50% of the fish occurs at a temperature of 39ºC, with and without the Mn presence. Furthermore, the catalase gene expression (oxidative stress) did not show alterations.

Body size dictates physiological and behavioural responses to hypoxia and elevated water temperatures in Murray cod (Maccullochella peelii).

Increasing drought frequency and duration pose a significant threat to fish species in dryland river systems. As ectotherms, fish thermal and hypoxia tolerances directly determine the capacity of species to persist in these environments during low flow periods when water temperatures are high and waterbodies become highly stratified. Chronic thermal stress can compound the impacts of acute hypoxic events on fish resulting in significant fish mortality; however, it is not known if all size classes are equally susceptible, or if the allometric scaling of physiological processes means some size classes are disproportionately affected. We investigated the physiological responses of Murray cod (Maccullochella peelii) over a four-fold body size range (0.2-3000g) to acute changes in water temperature and oxygen concentration following 4 weeks of acclimation to representative spring (20°C) and summer (28°C) water temperatures. We recorded maximum thermal tolerance (CT max), oxygen limited thermal tolerance (PCTmax ), lowest tolerable oxygen level (as the oxygen level at which lose equilibrium; O2,LOE), gill ventilation rates and aerial surface respiration threshold, blood oxygen transport capacity and lactate accumulation. Acclimation to elevated water temperatures improved thermal and hypoxia tolerance metrics across all size classes. However, body size significantly affected thermal and hypoxia responses. Small M. peelii were significantly less hypoxia tolerant than larger individuals, while larger fish were significantly less thermal tolerant than smaller fish. Hypoxia constrained thermal tolerance in M. peelii, with both small and large fish disproportionally compromised relative to mid-sized fish. Our findings indicate that both very small/young (larvae, fry, fingerlings) and very large/older M. peelii in dryland rivers are at significant risk from the combined impacts of a warming and drying climate and water extraction. These data will inform policy decisions that serve to balance competing demands on precious freshwater resources.

High proportion of RR-TB and mutations conferring RR outside of the RRDR of the rpoB gene detected in GeneXpert MTB/RIF assay positive pulmonary tuberculosis cases, in Addis Ababa, Ethiopia.

Globally, TB is the leading cause of infectious disease morbidity and mortality with many diagnostic uncertainties. Access to affordable and rapid diagnostics remained a major challenge for many developing countries which bear the greatest burden of TB delaying the initiation time to treatment. This study aimed to assess the GeneXpert MTBRIF assay probe utility for the detection of pulmonary TB and Rifampicin-resistant TB cases in Addis Ababa, Ethiopia. A cross-sectional study was performed from October 2019 to July 2020 in Saint Peter TB Specialized Hospital in Addis Ababa metropolitan area, Ethiopia. This study enrolled 216 clinically suspected new presumptive pulmonary TB cases confirmed by GeneXpert MTB/RIF Assay. Sociodemographic and clinical characteristics were captured using a structured tool. Data were entered in Microsoft Excel 2019, checked for inconsistency, cleaned promptly, and exported to IBM SPSS Statistics for Windows, Version 26.0. Armonk, N.Y: IBM Corp, the USA for analysis. Descriptive analysis and binary and multivariate logistics regression were performed and all statistical significance was determined at a 95% confidence level. The majority of the study participants, 55.1% [119/216] were males aged 6-80 years. The prevalence of RR MTB was 11.11% [24/216]. A higher proportion of RR TB was found in female patients [54.2%, 13/24], in patients in the age group of 30-50 years [45.8%, 11/24], in married individuals [62.5%, 15/24], in persons whose residence is urban [79.2%, 19/24], in persons who had a previous history of TB symptoms [100%, 24/24], in persons who had a history of contact with active and LTBI [33.3%, 8/24], and in persons who had a history of HIV and IDUs [41.7%, 10/24]. Occupation (AOR 22.868, 95% CI 1.655-316.022, p = 0.019), history of previous PTB+ (AOR 4.222, 95% CI 1.020-17.47, p = 0.047), and history of HIV and IDUs (AOR 4.733, 95% CI 1.416-15.819, p = 0.012) were independent predictors associated with RR-TB emergence. The commonest mutation 62.5% [15/24] was found in probe E (codons 529-533) region. There was no mutation associated with probe A (codons 507-511), probe B (codons 511-518), and probe C (codons 518-523) regions, as well as no combination of missed probes, was revealed. However, 12.5% [3/24] of RR TB patients were found without unidentified missed probe types detected outside of the RRDR. The delta Ct max was >4.0 and the highest proportion of 35.6% [77/216] RR TB was detected in samples of medium DNA load. The proportion of RR-TB we observed in this study was high. Similarly, a higher proportion of RR TB was detected outside of the RRDR. Moreover, a significant number of the GeneXpert MTB/RIF Assay probes were identified as unhybridized and this critical observation would mean that most of the probes had no or minimal utility in this geographical region. This calls for further studies to uncover mutation in the rpoB gene conferring RR and reshape TB triage and definite diagnostic algorithm in Ethiopia.

Thermal tolerance and critical maxima examined in marine gastropods inhabiting around Karachi Nuclear Power Plant

Bensthic communities along the coastal basins are an indication of ecosystem health but highly susceptible owing to manmade activities. This study envisages thermal tolerance in sea snailsMonodonta canalifera,Nerita albicillaandTylothais savignyiinhabiting around the outfall and intake structures of Karachi Nuclear Power Plant (KANUPP). To test the adaptability and vulnerability, a lethality test protocol under controlled temperature was applied in the laboratory where they were exposed at 25, 30, 35, and 40°C, which was raised to 45°C after an acclimation period of 1 week. The critical thermal maximum (CTmax) for the three species was found to be between 39 and 42°C, whereas the lethal temperature (LT50) tests revealed that at the utmost 45°C was lethal forM. canalifera. The correlation between LT50and CTmaxima(R= 0.47,p= 0.00) and LT50and body sizes reveals that the thermal adaptability inN. albicillaandT. savignyi(R= 0.65,p= 0.00) was relatively higher than that at 45°C given in the laboratory. In addition, microscopic changes due to temperature, which appeared in the foot (adhesive part) of each species, were deduced from the histological examination. The outcomes of this study would help to underline the ecosystem health around KANUPP and highlight precautionary measures required for the newly established K2/K3 power units to safeguard habitat.

Differences in the gut microbiome composition of Korean children and adult samples based on different DNA isolation kits.

Recent studies have revealed that the composition of human gut microbiota varies according to region, race, age, diet, living environment, and sampling and DNA extraction method. The purpose of this study was to broaden our understanding of the intestinal microbial composition of Koreans by conducting a 16S rRNA amplicon sequencing on 78 Korean samples composed of adults, children, normal and obese groups. We compared the microbiome composition and diversity of these groups at different levels including the phylum and genus level using two different stool DNA extraction kits of QIAamp® PowerFecal® DNA Kit (Qiagen, Hilden, Germany) and CT Max Fecal DNA Kit (Ct bio, Korea). We found that Ct bio (Ct) kit recovered higher DNA yields and OTUs than QIAamp® PowerFecal® DNA Kit (Qia). The Ct kit, which adopted more rigorous bead beating method, detected the most Gram-positive (G+) bacteria, Firmicutes, at the Phylum level, whereas the Qia kit, which used a less rigorous cell lysis method, found the most Gram-negative (G-) bacteria, Bacteroidetes. The Firmicutes-to-Bacteroidetes (F/B) ratio showed no significant difference between the obese and the normal groups of same kit; however, they were significantly different with two different kits. There was a difference in the intestinal flora between healthy Korean adults and children. The taxa that differed significantly between the adults and children were Bacteroides, Bifidobacterium, Prevotella, and Subdoligranulum. There was no significant difference in the intestinal flora between the normal weight group and the obese group in adults and children, respectively. This is probably because the difference in body mass index (BMI) between the sample groups collected in this study is statistically significant, but it is not large enough to show a clear difference in the flora. Therefore, these results should be interpreted with caution while considering the BMI values and Korean obesity criterion together.

Physiological, developmental, and behavioral plasticity in response to thermal acclimation

Organisms with complex ecologies and life-cycle processes may shift physiologically (acclimation in tolerance), developmentally, and/or behaviorally (thermoregulation) in response to changes in climate. As such, climate change may trigger multiple, interacting phenotypic responses, which underscores the nuances of characterizing a species capacity to adapt and respond to climate change. In this study, we use a model frog species, Bufo gargarizans, to examine how three phenotypes, thermal tolerance limits (critical thermal minimum, CTmin and critical thermal maximum, CTmax), ontogeny, and behavioral preferences in temperature (Tpref) respond to different levels of thermal exposure (i.e., acclimation ranging from 10°C to 30°C). Acclimation temperature had little effect on Tpref of tadpoles, yet behaviorally they showed strong signs of thermal selection towards an optimum. Both CTmin and CTmax increased with acclimation temperature with an approximate 10% increase in tolerance limits per 1°C increase in exposure. Development and body size both responded to acclimation temperature, both of which also influenced lower but not upper thermal limits. Our study highlights the idiosyncrasies of estimating climate vulnerability, where multiple phenotypes can respond to shifts in temperature-a complexity that is especially apparent in species with complex life-cycles.

The effect of spacing between inner blades on the performance of the Savonius wind turbine

Savonius wind turbine is an interesting type of vertical axis wind turbines (VAWTs) which has a low cost of manufacturing, however, this turbine has a relatively low power output compared to the other types of VAWTs. Further study is required to enhance its performance to match the high demand of the power generation in small-scale applications. The main objective of the current study is to enhance the power output coefficient (Cp) of the conventional Savonius rotor by adding two-inner blades to the geometry. The performance assessment was performed for five different angles of inner blades (100°, 120°, 140°, 160°, and 180°). Moreover, the effect of spacing between inner blades was numerically examined for three values of spacing i.e. 0.02, 0.01, and 0.005 m. The current two-dimensional simulation was performed utilizing the Ansys Fluent solver. The simulation results affirmed that the maximum enhancement to be 32.9% compared to the conventional rotor at TSR equal to 0.7 when the spacing between inner blades is 0.005 m with an inner blades angle of 100°. Moreover, results demonstrated that the Ct max is 0.435 at TSR = 0.4 for the rotor with 160° inner blades and a spacing of 0.005 m.

Influence of Non-lethal Doses of Natural Insecticides Spinetoram and Azadirachtin on Helicoverpa punctigera (Native Budworm, Lepidoptera: Noctuidae) Under Laboratory Conditions.

Helicoverpa punctigera (native budworm) is an important pest species in crops across Australia. From the third instar onward, this species causes severe damage to crop plants: therefore, caterpillars need to be managed at an early stage of their development. In our experiment, we raised H. punctigera on an artificial diet, which included different concentrations of the natural insecticides Spinetoram and Azadirachtin. The survival of the larvae, growth and body mass gain was recorded over 17 days. Only caterpillars raised on lowest toxin concentrations survived and molted successfully to the fifth instar, but had slower growth and body mass gain compared to the insecticide-free control group. Caterpillars fed on higher toxin concentrations never molted to the next instar or died in the first few days. To test how the toxins influence physiological conditions including metabolic rate and water loss, surviving fifth instar larvae were exposed to thermolimit respirometry: starting at 25°C following a constant increasing temperature ramping rate of 0.25°C–1, until reaching the critical thermal maxima (CTmax). Caterpillars raised on a non-lethal dose of insecticides had higher metabolic rates and lost more water compared to the control group. Insects that have seem to consume more energy per mg tissue and have a higher water loss at high temperatures. Non-lethal concentrations of insecticides on pest insects physiology may reduce their impact on crops and may enable more targetted insecticide application.

Does Xpert\xae MTB/RIF assay give rifampicin resistance results without identified mutation? Review of cases from Addis Ababa, Ethiopia

BackgroundXpert® MTB/RIF assay is currently used in Ethiopia for the rapid diagnosis of Mycobacterium tuberculosis (MTB) and mutations that confer Rifampicin resistance. Rifampicin resistance is determined based on any mutation in the 81 bp of rpoB gene using five overlapping probes represented as Probe A (codons 507–511), Probe B (codons 512–518), Probe C (codons 518–523), Probe D (codons 523–529) and Probe E (codons 529–533). In this review, we assessed the frequency of missed probe types for Rifampicin Resistance results.MethodsData were reviewed from specimens received and tested using Xpert® MTB/RIF assay at Ethiopian National Tuberculosis Reference Laboratory, in Addis Ababa from 15 July 2016 to 31 December 2018 retrospectively. All archived data were reviewed carefully to describe missed probe types and the quantity of DNA in the sample.ResultsA total of 100 specimens were reported as MTB Detected Rifampicin Resistance Detected by Xpert® MTB/RIF assay. More than half (55%) of these results were reported from male patients. The median age was 28.0 years (5 months to 88 years). Majorities (62%) of the cases were detected from sputum. Among the total of 38 extrapulmonary samples, lymph node aspirates were accounted for 50% (19/38). The most common mutations (81.0%) were found in the Probe E region followed by Probe D (10.0%), and Probe B (3.0%). Mutations in Probe A and Probe C regions were not observed. However, six (6.0%) Rifampicin resistance cases were found without any missed probe type. The delta Ct max is ≥4.3. No specimen yielded Rifampicin resistance associated with more than one probe failure or mutation combinations.ConclusionMutations associated with Probe E (codons 529–533) region were identified as the commonest rpoB gene mutations. The Rifampicin resistance results found without any identified missing probe needs further study. The lower DNA amount was observed in extrapulmonary specimens compared with sputum.

Prospective Evaluation Fiducial Migration after Placement for Liver SBRT and Influence on Treatment Delivery

Fiducial placement in liver tumors (HCC) for Robotic Radiosurgery (CK) treatment is critical. There is a possibility of migration and related issues. Present study evaluating the impact of fiducial migration after simulation and during treatment. Between Mar 2017-Mar 2018, 53 HCCs accrued in the ethical & scientific committee approved prospective study for CK treatment. Three fiducials were place in liver (CT/USG guided) for tracking. Fiducials placed by radiologist with ‘ideal’ fiducials will be equidistance from each other (max distance 5 cm, min distance 2 cm). CT scan imaging was done at immediate post-placement (D0), at Day-3 and Day-8 to evaluate migration. During treatment (Day-4-8) intra-fraction imaging done to track the fiducials position. Fifty three patients (Male 92%, mean age 60.2 yrs, ECOG 0-1 92%, Child-Pugh A 89%, B&C 11%, majority in seg II & VI, PVT disease 64%) with HCCs underwent fiducial placement under guidance (CT scan 69%). Among 159 fiducial placement, six (3.4%)[lung 2, spleen 1, Heart 1, abdomen 2] had displacement outside liver at immediate post-fiducial period (Day-0). Day-0 CT scan, min and max inter-fudicial distance, min & max distance from tumor, max & min inter-fiducial angle are 2.66 (0.819) & 4.8 (1.08) mm; 2.1 (0.7) & 5.12 (1.25) mm; 28.5 (11.8) & 83 (25.25) degree respectively. Maximum and minimum fiducial migration on Day 3 was 0.85 (0.22) and 028 (1.04) mm. Tracking range, co-linearity, tracking uncertainty on Day1-5 of treatment was 5.07, 4.5, 4.9, 5.1, 5.1; 27.02, 25.9, 27.2, 27.6, 27.8; 40.9, 44.3, 47.3, 49.7, 47.06 respectively. Maximum migration during treatment (Day 1-5) was 0.29, 0.27, 0.27, 0.28, and 0.31 respectively. Total migration after treatment (Day 8) from day 0 was 2.35 (0.271) mm. Migration score evaluated on Day 3 and Day 8 did not show any significant difference in fiducial position compared with day 0. Displacement of fiducials occurs at immediate post-fiducial period. There is no significant change in fiducial positions at Day-3 and Day 8 from baseline (D-0). Liver SBRT treatment after fiducial placement may be started immediate post-fiducial placement (Day 1). No additional advantage of waiting for 3 days or more for fiducial stabilization.

Pulmonary F-18 FDG uptake of non-tumor area in lung cancer patients to provide risk stratification in postoperative acute exacerbation of interstitial lung disease.

e13165 Background: The incidence of lung cancer in patients with idiopathic pulmonary fibrosis (IPF) is higher than the general population. In IPF patients with lung cancer, postoperative acute exacerbation (AE) is considered a fatal comorbidity. This study aimed to investigate whether FDG-PET/CT can be used to assess the risk of postoperative AE of IPF and severe respiratory adverse events (SRAE),which are defined as grade 3a or more severe respiratory complications such as AE, bacterial pneumonia, empyema, and bronchial fistula based upon Clavian-Dindo classification. Methods: We identified 822 patients with lung cancer who had preoperative FDG-PET/CT and subsequent pulmonary resection from July 2012 to July 2018. We reviewed the patients’ clinical records and transferredthe preoperative FDG-PET/CT images of each patient to SynapseVincent system (Fujifilm Corporation, Tokyo, Japan) for measuring SUV value of the lung.Inevery case, both SUVmax of main tumor (tumor-SUVmax) and non-lung tumor part (NLT-SUVmax) were measured. Univariate and multivariate analyses were performed for exploring significant parameters associated with AE and SRAE. Results: The cohort in this study consisted of 460 men and 362 women (mean age 67.9 ± 10.1 years; range 23–87). SRAE was observed in 35 (4.2%) patients including those with AE (n = 16, 1.9%). NLT-SUVmax and presence of IPF on CT were the independent predictive factors for both AE and SRAE. The receiver operating characteristic (ROC) area under the curve (AUC) of significant factors associated with AE and SRAE was measured, and cut-off levels on ROC curves for significant parameters associated with AE and SRAE were 1.69 and 1.55, respectively. The risk rates for the incidence of AE and SRAE according to NLT-SUVmax and chest CT findings were 19.0 % and 30.2% of the patients with positive IPF on CT and NLT-SUV max ≥1.69. Conclusions: NLT-SUVmax was the independent predictor of the incidence of AE and SRAE for patients who underwent pulmonary resection. FDG/PET-CT can be used in routine clinical practice to identify high-risk individuals for AE who require careful and intensive observation after pulmonary resection and that can facilitate clinical decision-making.

Thermal niche conservatism in an environmental gradient in the spider Sicarius thomisoides (Araneae: Sicariidae): Implications for microhabitat selection

Temperature is one of the most important environmental variables for organisms, especially for ectothermic animals. In fact, ectotherms must move within a relatively narrow range of temperatures where they are able to maximize their performance. We assessed the thermal ecology of female sand spiders (Sicarius thomisoides) in Chile from separate populations along an environmental gradient and different macro habitats (coast vs. inland locations). The parameters of thermal performance curves do not vary between populations, with an average optimum temperature (T°opt) of 25.33 ± 2.65 °C, and a CT min and CT max of 6.56 ± 1.72 °C and 44.23 ± 4.92 °C, respectively. Our results show that the thermal niche in laboratory is conserved and does not vary along an environmental gradient coinciding with the temperatures selected by female spiders in their microhabitats.

Comparison of Aerobic Scope for Metabolic Activity in Aquatic Ectotherms With Temperature Related Metabolic Stimulation: A Novel Approach for Aerobic Power Budget.

Considering that swim-flume or chasing methods fail in the estimation of maximum metabolic rate and in the estimation of Aerobic Scope (AS) of sedentary or sluggish aquatic ectotherms, we propose a novel conceptual approach in which high metabolic rates can be obtained through stimulation of organism metabolic activity using high and low non-lethal temperatures that induce high (HMR) and low metabolic rates (LMR), This method was defined as TIMR: Temperature Induced Metabolic Rate, designed to obtain an aerobic power budget based on temperature-induced metabolic scope which may mirror thermal metabolic scope (TMS = HMR—LMR). Prior to use, the researcher should know the critical thermal maximum (CT max) and minimum (CT min) of animals, and calculate temperature TIMR max (at temperatures −5–10% below CT max) and TIMR min (at temperatures +5–10% above CT min), or choose a high and low non-lethal temperature that provoke a higher and lower metabolic rate than observed in routine conditions. Two sets of experiments were carried out. The first compared swim-flume open respirometry and the TIMR protocol using Centropomus undecimalis (snook), an endurance swimmer, acclimated at different temperatures. Results showed that independent of the method used and of the magnitude of the metabolic response, a similar relationship between maximum metabolic budget and acclimation temperature was observed, demonstrating that the TIMR method allows the identification of TMS. The second evaluated the effect of acclimation temperature in snook, semi-sedentary yellow tail (Ocyurus chrysurus), and sedentary clownfish (Amphiprion ocellaris), using TIMR and the chasing method. Both methods produced similar maximum metabolic rates in snook and yellowtail fish, but strong differences became visible in clownfish. In clownfish, the TIMR method led to a significantly higher TMS than the chasing method indicating that chasing may not fully exploit the aerobic power budget in sedentary species. Thus, the TIMR method provides an alternative way to estimate the difference between high and low metabolic activity under different acclimation conditions that, although not equivalent to AS may allow the standardized estimation of TMS that is relevant for sedentary species where measurement of AS via maximal swimming is inappropriate.

Oxidative stress and antioxidant defense responses of Etroplus suratensis to acute temperature fluctuations

Fishes are always exposed to various environmental stresses and the chances of succumbing to such stresses are of great physiological concern. Any change in temperature from the ambient condition can induce various metabolic and physiological changes in the body. The present study evaluates the effects of temperature induced stress on the antioxidant profile of Etroplus suratensis such as superoxide dismutase, catalase, glutathione peroxidase and lipid peroxidation. Fishes of same size were kept in a thermostatized bath at three different temperature regimes viz 16°C, 27°C (ambient temperature) and 38°C for 72h. These temperatures were selected based on the CT Max (Critical Thermal Maximum) and CT Min (Critical Thermal Minimum) exhibited by E. suratensis. Superoxide dismutase and catalase activity was found maximum in brain and muscle respectively during the 48th hour of exposure in fishes kept at 38°C. At 16°C the antioxidant response of glutathione peroxidase was maximum in muscles, whereas the lipid peroxidation rate was found to be high in gills compared to other tissues. The profound increase in the levels of oxidative stress related biomarkers indicate that the thermal stressors severely affected oxidative state of E. suratensis by the second day of experiment. Such down-regulation of redox state accompanied with the induction of oxidative stress cascade may lead to physiological damage in various tissues in fishes, in vivo. However current data indicate that a transition to low and high temperature environment from ambient condition severely affected the levels and profile of the antioxidant markers overtime in E. suratensis.

Divergence of thermal physiological traits in terrestrial breeding frogs along a tropical elevational gradient.

Critical thermal limits are thought to be correlated with the elevational distribution of species living in tropical montane regions, but with upper limits being relatively invariant compared to lower limits. To test this hypothesis, we examined the variation of thermal physiological traits in a group of terrestrial breeding frogs (Craugastoridae) distributed along a tropical elevational gradient. We measured the critical thermal maximum (CT max; n = 22 species) and critical thermal minimum (CT min; n = 14 species) of frogs captured between the Amazon floodplain (250 m asl) and the high Andes (3,800 m asl). After inferring a multilocus species tree, we conducted a phylogenetically informed test of whether body size, body mass, and elevation contributed to the observed variation in CT max and CT min along the gradient. We also tested whether CT max and CT min exhibit different rates of change given that critical thermal limits (and their plasticity) may have evolved differently in response to different temperature constraints along the gradient. Variation of critical thermal traits was significantly correlated with species’ elevational midpoint, their maximum and minimum elevations, as well as the maximum air temperature and the maximum operative temperature as measured across this gradient. Both thermal limits showed substantial variation, but CT min exhibited relatively faster rates of change than CT max, as observed in other taxa. Nonetheless, our findings call for caution in assuming inflexibility of upper thermal limits and underscore the value of collecting additional empirical data on species’ thermal physiology across elevational gradients.

Desiccation resistance in tropical insects: causes and mechanisms underlying variability in a Panama ant community.

Desiccation resistance, the ability of an organism to reduce water loss, is an essential trait in arid habitats. Drought frequency in tropical regions is predicted to increase with climate change, and small ectotherms are often under a strong desiccation risk. We tested hypotheses regarding the underexplored desiccation potential of tropical insects. We measured desiccation resistance in 82 ant species from a Panama rainforest by recording the time ants can survive desiccation stress. Species' desiccation resistance ranged from 0.7 h to 97.9 h. We tested the desiccation adaptation hypothesis, which predicts higher desiccation resistance in habitats with higher vapor pressure deficit (VPD) – the drying power of the air. In a Panama rainforest, canopy microclimates averaged a VPD of 0.43 kPa, compared to a VPD of 0.05 kPa in the understory. Canopy ants averaged desiccation resistances 2.8 times higher than the understory ants. We tested a number of mechanisms to account for desiccation resistance. Smaller insects should desiccate faster given their higher surface area to volume ratio. Desiccation resistance increased with ant mass, and canopy ants averaged 16% heavier than the understory ants. A second way to increase desiccation resistance is to carry more water. Water content was on average 2.5% higher in canopy ants, but total water content was not a good predictor of ant desiccation resistance or critical thermal maximum (CT max), a measure of an ant's thermal tolerance. In canopy ants, desiccation resistance and CT max were inversely related, suggesting a tradeoff, while the two were positively correlated in understory ants. This is the first community level test of desiccation adaptation hypothesis in tropical insects. Tropical forests do contain desiccation‐resistant species, and while we cannot predict those simply based on their body size, high levels of desiccation resistance are always associated with the tropical canopy.

Dietary pyridoxine potentiates thermal tolerance, heat shock protein and protect against cellular stress of Milkfish (Chanos chanos) under endosulfan-induced stress

We herein report the protective role of pyridoxine in enhancing thermal tolerance of Milkfish Chanos chanos reared under endosulfan-induced stress. Four isocaloric and isonitrogenous diets were prepared with graded levels of pyridoxine (0, 50, 75 and 100mg/kg). Two hundred and twenty five fishes were randomly distributed into four treatment groups in triplicate, reared under endosulfan-treated water, which were fed with pyridoxine supplemented diet, while the negative control group was reared without endosulfan-treatment and control fed. The concentration of endosulfan in treated water was maintained at a level of 1/40th of LC50 i.e. 0.52μg/L. Dietary pyridoxine supplementation had significant (p<0.01) effect on temperature tolerance viz. CTmax (Critical temperature maxima), LTmax (Lethal temperature maxima), CTmin (Critical temperature minima) and LTmin (Lethal temperature minima) of milkfish. The positive correlation was observed between CT max and LTmax (Y=-1.54+15.6x, R(2), 0.943) as well as CTmin and LTmin (Y=-1.44+1.021x, R(2), 0.941). At the end of the thermal tolerance study, antioxidative status and HSP 70 were significantly reduced in pyridoxine supplemented groups, whereas brain AChE was significantly (p<0.01) elevated compared to positive and negative control. It is concluded that CTmax, LTmax, CTmin and LTmin, antioxidative status, neurotransmitter enzyme and HSP 70 strengthened the enhancement of thermal tolerance of Milkfish.

Effects of Loma morhua (Microsporidia) infection on the cardiorespiratory performance of Atlantic cod Gadus morhua (L).

The microsporidian Loma morhua infects Atlantic cod (Gadus morhua) in the wild and in culture and results in the formation of xenomas within the gill filaments, heart and spleen. Given the importance of the two former organs to metabolic capacity and thermal tolerance, the cardiorespiratory performance of cod with a naturally acquired infection of Loma was measured during an acute temperature increase (2 °C h(-1)) from 10 °C to the fish's critical thermal maximum (CT(Max)). In addition, oxygen consumption and swimming performance were measured during two successive critical swimming speed (U(crit)) tests at 10 °C. While Loma infection had a negative impact on cod cardiac function at warm temperatures, and on metabolic capacity in both the CT(Max) and U(crit) tests (i.e. a reduction of 30-40%), it appears that the Atlantic cod can largely compensate for these Loma-induced cardiorespiratory limitations. For example, (i) CT(Max) (21.0 ± 0.3 °C) and U(crit) (~1.75 BL s(-1)) were very comparable to those reported in previous studies using uninfected fish from the same founder population; and (ii) our data suggest that tissue oxygen extraction, and potentially the capacity for anaerobic metabolism, is enhanced in fish infected with this microsporidian.

Experimental manipulations of tissue oxygen supply do not affect warming tolerance of European perch.

A progressive inability of the cardiorespiratory system to maintain systemic oxygen supply at elevated temperatures has been suggested to reduce aerobic scope and the upper thermal limit of aquatic ectotherms. However, few studies have directly investigated the dependence of thermal limits on oxygen transport capacity. By manipulating oxygen availability (via environmental hyperoxia) and blood oxygen carrying capacity (via experimentally induced anaemia) in European perch (Perca fluviatilis Linneaus), we investigated the effects of oxygen transport capacity on aerobic scope and the critical thermal maximum (CT(max)). Hyperoxia resulted in a twofold increase in aerobic scope at the control temperature of 23°C, but this did not translate to an elevated CT(max) in comparison with control fish (34.6±0.1 versus 34.0±0.5°C, respectively). Anaemia (∼43% reduction in haemoglobin concentration) did not cause a reduction in aerobic scope or CT(max) (33.8±0.3°C) compared with control fish. Additionally, oxygen consumption rates of anaemic perch during thermal ramping increased in a similar exponential manner to that in control fish, highlighting that perch have an impressive capacity to compensate for a substantial reduction in blood oxygen carrying capacity. Taken together, these results indicate that oxygen limitation is not a universal mechanism determining the CT(max) of fishes.

Dietary lecithin potentiates thermal tolerance and cellular stress protection of milk fish (Chanos Chanos) reared under low dose endosulfan-induced stress

Endosulfan is an organochlorine pesticide commonly found in aquatic environments that has been found to reduce thermal tolerance of fish. Lipotropes such as the food additive, Lecithin has been shown to improve thermal tolerance in fish species. This study was conducted to evaluate the role of lipotropes (lecithin) for enhancing the thermal tolerance of Chanos chanos reared under sublethal low dose endosulfan-induced stress. Two hundred and twenty-five fish were distributed randomly into five treatments, each with three replicates. Four isocaloric and isonitrogenous diets were prepared with graded levels of lecithin: normal water and fed with control diet (En0/L0), endosulfan-treated water and fed with control diet (En/L0), endosulfan-treated water and fed with 1% (En/L1%), 1.5% (En/L 1.5%) and 2% (En/L 2%) lecithin supplemented feed. The endosulfan in treated water was maintained at the level of 1/40th of LC50 (0.52ppb). At the end of the five weeks, critical temperature maxima (CTmax), lethal temperature maxima (LTmax), critical temperature minima (CTmin) and lethal temperature minima (LTmin) were Determined. There was a significant (P<0.01) effect of dietary lecithin on temperature tolerance (CTmax, LTmax, CTmin and LTmin) of the groups fed with 1, 1.5 and 2% lecithin-supplemented diet compared to control and endosulfan-exposed groups. Positive correlations were observed between CT max and LTmax (R2=0.934) as well as between CTmin and LTmin (R2=0.9313). At the end of the thermal tolerance study, endosulfan-induced changes in cellular stress enzymes (Catalase, SOD and GST in liver and gill and neurotansmitter enzyme, brain AChE) were significantly (p<0.01) improved by dietary lecithin. We herein report the role of lecithin in enhancing the thermal tolerance and protection against cellular stress in fish exposed to an organochlorine pesticide.