1. 1. A Study has been carried out in order to explain the enzyme-palmitoleate interaction. 2. 2. The highly purified and crystalline enzymes representative of fundamental metabolic pathways were: alcohol dehydrogenase (ADH), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), isocitrate dehydrogenase (ICDH), glucose-6-phosphate dehydrogenase (G6P-DH), alkaline phosphatase. 3. 3. The enzyme-palmitoleate interaction was studied as a phenomenon time-independent (inhibition) and time-dependent (inactivation). 4. 4. Palmitoleate inhibited remarkably LDH, MDH, ICDH and G6P-DH. 5. 5. A kinetic analysis of the inhibitory action of palmitoleate on LDH and MDH was also carried out. 6. 6. Inactivation studies have shown that ADH and alkaline phosphatase are not sensitive to palmitoleate action, unlike the other enzymes. 7. 7. A comparison was made between the action of palmitoleate and that of a synthetic anionic detergent, sodium dodecyl sulfate (SDS).