Isolation of environmental DNA (eDNA) is becoming a valuable tool for detecting presence of rare or secretive aquatic species. The recent use of quantitative PCR (qPCR) with eDNA sampling presents the possibility of using this method to infer population abundance and status. This approach would be especially useful for species such as the Eastern hellbender (Cryptobranchus alleganiensis alleganiensis), a declining, secretive, aquatic salamander that requires intense field survey effort to study. In 2012, we conducted eDNA sampling at sites across the range of the species in North Carolina. Our objectives were to assess presence across 61 sites, test for a correlation of abundance and biomass with eDNA estimates at a subset of 23 sites, and sample at multiple spatial and temporal scales in three river systems. Overall, we detected hellbender eDNA at 33 sites, including all sites with 2012 hellbender records, 71% of all recent or historic sites with hellbender presence, and at nine sites that lack species occurrence records. We did not find a correlation between eDNA estimates and field survey counts of individuals or biomass. We detected a strong temporal increase in eDNA during the September breeding period, but no consistent evidence of a spatial relationship with eDNA. Overall, our results demonstrate the efficacy of eDNA for detecting hellbender populations. Furthermore, the potential utility of qPCR to assess population status in hellbenders requires further study and testing, although it may be promising for determining population reproductive status.