Crypt Depth Research Articles

Comparative evaluation of Acheta domesticus and Hermetia illucens as alternative protein sources for the growth, health, and meat quality of the broiler

IntroductionInsects are receiving increasing attention due to their potential to enhance farming efficiency and sustainability, mitigate vast quantities of bio-waste, and improve animal performance. It is hypothesized that replacing soybean meal with Acheta domesticus will improve broiler productive performance, health, and meat quality. Therefore, the objective of this study was to assess the effect of the gradual replacement of soybean meal (4%, 8%, and 12%) with house cricket (A. domesticus) and black soldier fly (Hermetia illucens) on productive performances, hematology, intestinal morphology, and meat quality attributes of male broiler (Ross 308). MethodsA total of 350 1-day-old chicks (39.23 ± 0.19 g) were divided into seven groups (five pens per group and 10 chicks per pen) following a completely randomized design. The seven groups included 4%, 8%, and 12% SBM replacements with A. domesticus and H. illucens. Soybean meal was the basal diet considered the control. ResultsThe broilers fed 12% A. domesticus, or 12% H. illucens had significantly higher ((p 0.05) live weight, average daily weight gain, and improved feed conversion ratio than the broilers fed basal (SBM) diet throughout starter, grower, and finisher phases. Hematology (complete blood count and serum biochemistry traits) and intestinal morphology (villus height, villus width, crypt depth, and villus height-to-crypt depth ratio) of the broilers improved when fed on 12% A. domesticus and 12% H. illucens meals as compared to the control group (p < 0.05). Feeding 12% A. domesticus and 12% H. illucens also had a significant positive impact on the meat quality traits of broilers, such as maximum redness (a*) and yellowness (b*) with minimal cooking loss and lightness (L*) (p < 0.05) than the broilers fed the control diet. DiscussionUsing insect meal as an alternative to traditional feed sources could contribute significantly to the sustainable expansion of the poultry industry.

The potential of supplementing compound organic trace elements at lower levels in Chinese yellow- feathered broiler diets, part II: Impacts on growth performance, gut health, intestinal microbiota, and fecal mineral excretion.

This study aimed to investigate the effects of reducing inorganic trace minerals (ITM) by supplementing compound organic trace minerals (OTM) chelates on growth performance, fecal mineral excretion, intestinal health, and cecal microbiota of yellow-feathered broilers. A total of 960 one day old male broilers were randomly assigned to 6 treatments, among which birds were fed with the basal diets (negative control, NC), or supplemented with 1,000 mg/kg (positive control, PC), 300, and 500 mg/kg ITM or OTM, respectively. Dietary supplementation of OTM significantly increased the average daily gain (ADG) during 22-53 d and 1-53 d, and reduced the fecal emissions of Fe, Cu, Zn, and Mn of Chinese yellow-feathered broilers (P < 0.05). Furthermore, the OTM300 group significantly reduced the crypt depth in the duodenum, and increased the ratio of villus height to crypt depth (V/C) in the duodenum and jejunum (P < 0.05). The mRNA expression of TGF-β, Bcl-2, CAT, and GPX4 as well as tight junction proteins (occludin, ZO-1, claudin-1, and claudin-5) in jejunum mucosa were significantly increased by compound OTM when comparing with ITM300 group (P < 0.05). Moreover, dietary compound OTM significantly changed the Chao1 index and β diversity index of cecal microbiota of Chinese yellow-feathered broilers. The abundances of Firmicutes (phylum), Eubacterium_coprostanoligenes_group (family) and Oscillibacter (genus) were increased, while the abundances of Bacteroidetes (phylum) and Rikenellaceae RC9 group (genus) were decreased by OTM treatment. Spearman correlation analysis showed that the mRNA of occludin and jejunal V/C ratio were positively correlated with the abundance of Firmicutes (phylum), but negatively correlated with the abundance of Bacteroidota (phylum). In addition, the abundance of Eubacterium_coprostanoligenes_group (family) was positively correlated with the mRNA of claudin-1, Bcl-2, and TGF-β. PICRUST prediction of microbial function revealed that OTM treatment enriched the pathways related to amino acid metabolism and DNA replication. In conclusion, dietary supplementation at lower levels of compound OTM to replace ITM could improve growth performance and intestinal health, and reduce the fecal excretion of trace elements by modulation of cecal microbiota community and diversity in Chinese yellow-feathered broilers.

Impact of fermented bamboo powder on the morphology and physiology of the gastrointestinal tract in yellow-feather broiler chickens.

Bamboo powder, a novel ingredient, is gaining recognition for its potential as a dietary supplement in poultry feed. This study aimed to investigate the effects of fermented bamboo powder (FBP) on antioxidant status, gut hormone activities, intestinal digestive enzyme activities, gut morphological structure, gastrointestinal development, and the expression of nutritional transporter genes in dwarf yellow-feather broiler chickens. A total of 600 healthy 1-day-old chicks were allocated randomly into two groups, with 10 replicates per group and 30 chicks in each replicate. The control group was provided with a standard basal diet, whereas the experimental group received the same basal diet supplemented with 1.0, 2.0, 4.0, and 6.0 g/kg of fermented bamboo powder (FBP) in four phases: Phase I (days 1-22), Phase II (days 23-45), Phase III (days 46-60), and Phase IV (days 61-77). Phases I and II were categorized as the pretreatment period (days 0-45), while Phases III and IV represented the experimental period (days 46-77). Tissue samples were collected during Phase IV for further analysis. After 77 days of feeding, results revealed that FBP supplementation significantly enhanced the levels of gastrointestinal hormones (Glucagon-like peptide 1, Peptide YY, Cholecystokinin, and 5-hydroxytryptamine) in the duodenum, jejunum, and ileum. Similarly, the activities of digestive enzymes (protease, chymotrypsin, trypsin, and amylase) were significantly increased in the small intestine. It also improved gut morphology by increasing villus height, crypt depth, and goblet cell counts in the duodenum, jejunum, and ileum. Additionally, antioxidant enzyme activities (superoxide dismutase, glutathione peroxidase, and catalase) were significantly increased, while malondialdehyde content was significantly decreased in the jejunum. Additionally, FBP supplementation significantly enhanced gizzard development. Overall, FBP supplementation modulated gut hormones and enzymes, enhanced gut morphology and promoted antioxidant status and gene expression related to nutrient transport and antioxidant defenses in broiler chickens. These findings suggest that FBP has the potential as a beneficial dietary supplement in poultry feed.

Effects of drinking water supplemented with essential oils and organic acids mixtures on growth performance and intestinal health of broilers challenged with necrotic enteritis.

It is urgent to develop effective antibiotic alternatives for the control of subclinical necrotic enteritis (NE) in chickens after in-feed antibiotics have been banned. The current study investigated the efficacy of drinking water supplemented with essential oils and organic acids mixtures (EOA) on growth performance and intestinal health of broilers challenged with necrotic enteritis (NE). A total of 360 one-day-old Arbor Acres male broilers were randomly divided into 5 treatment groups, including non-challenged control group (T0), challenged NE group (T1), and challenged NE chickens treated with 0.2 % EOA1 (T2) or 0.2 % EOA2 (T3) in drinking water, along with NE-challenged chickens treated with 45 mg/kg bacitracin methylene disalicylate (BMD) in the diet (T4). Results showed that drinking water supplemented with either EOA1 or EOA2 significantly decreased Clostridium perfringens load in ileal content (P < 0.05). EOA2 markedly reduced jejunal crypt depth, serum lipopolysaccharide (LPS) content, ileal IL-1β mRNA level and myeloperoxidase (MPO) activity, significantly increased Mucin-2 mRNA abundance in ileum of NE infected broilers (P < 0.05) when compared with single NE-infected group. The 16S sequencing analysis revealed that, compared with single NE-challenge group and the antibiotic BMD group, the addition of EOA1 in drinking water significantly increased the Shannon index and Simpson index of ileal microbiota in NE-infected broilers (P < 0.05), while drinking water supplemented with either EOA1 or EOA2 significantly decreased Streptococcus relative abundance of NE-infected broilers (P < 0.05). In summary, drinking water with EOA2 might alleviate the intestinal injury induced by NE challenge, and the gut health-improving effects of EOA2 were better than that of EOA1.

Protective effects of carnosic acid on growth performance, intestinal barrier, and cecal microbiota in yellow-feathered broilers under lipopolysaccharide challenge.

This research was performed to investigate protective effects of carnosic acid on growth performance, intestinal barrier, and cecal microbiota of lipopolysaccharide-challenged broilers. Three hundred 1-day-old yellow-feathered broilers (male) were allocated randomly into 5 treatments, with 6 replicates per treatment, and 10 birds per replicate cage. Birds in both the control group (CON) and the lipopolysaccharide-challenged group were provided with a basal diet, while others were fed a basal diet supplemented with 20, 40, and 60 mg/kg carnosic acid (CA20, CA40, CA60), respectively. At 17, 19, and 21 days of age, birds were injected intraperitoneally with lipopolysaccharide (500 μg/kg body weight), except those in CON, which were injected with saline. Compared with challenged birds, the CA20, CA40, and CA60 increased (P < 0.05) the final body weight, average daily gain, and average daily feed intake, and the CA40 and CA60 also decreased diarrhea rate. Compared with challenged birds, carnosic acid reduced (P < 0.05) plasmal levels of D-lactic acid and endotoxin, increased (P < 0.05) the villus height to crypt depth ratio, and the number of goblet cells in duodenum. The CA40 and CA60 elevated (P < 0.05) relative expression of cell junction proteins (Claudin-1/-2 and ZO-1/-2/-3) and MUC-2 in duodenum, while decreased (P < 0.05) relative expression of TLR2, TLR4, and the concentrations of IL-6, IL-10, TNF-α, TGF-β1 in duodenum. CA40 also increased (P < 0.05) the α-diversity of the cecal microbiota and boosted (P < 0.05) the relative abundance of beneficial phyla and genera, particularly Firmicutes, Anaerofilum, and Papilibacter. In conclusion, dietary supplementation with carnosic acid showed protective effects on the growth performance and intestinal health in challenged broilers by down-regulating the expression of TLRs (TLR2/4) and inhibiting the production of inflammatory cytokines, strengthening the tight junction in intestinal epithelial cells, and enhancing the diversity of microbiota and the relative abundance of beneficial bacteria. When supplemented to diet of broilers, 40 mg/kg carnosic acid was recommended.

Effects of tannic acid on growth performance, intestinal health, and tolerance in broiler chickens.

This study investigated the optimal tannic acid dosage and assessed tolerance levels in broiler chickens. In experiment 1, 525 broilers were randomly divided into 5 treatment groups, the control group (CON group) and groups TA1 to TA4, corresponding to treatments of 0.025, 0.05, 0.075, and 0.1 % tannic acid, respectively, to determine the effect of tannic acid on broiler growth performance and gut health. Experiment 2 was performed to evaluate the tolerance of tannic acid; 416 broilers were randomly divided into control (CTR), 0.075 % tannic acid (TA), 0.375 % tannic acid (5TA), and 0.75 % tannic acid (10TA) groups. In the first experiment, compared with that in the CON group, the growth performance and the ileal intestinal villi height to crypt depth ratio showed a quadratic curve increase with tannic acid supplementation (P < 0.05). Adding 0.05 % to 0.075 % tannic acid significantly improved the growth performance, intestinal morphology, and intestinal barrier function (P < 0.05). Tannic acid concentrations of 0.075 % significantly increased the abundance of Firmicutes and Lactobacillaceae in the ileum and decreased the abundance of Vibrionaceae and Yersiniaceae (P < 0.05). In experiment 2, the growth performance of the TA group significantly improved compared with that of the CTR group (P < 0.05). The F/G was significantly higher in the 5TA and 10TA groups than in the CTR group (P < 0.05), and the 10TA group had significantly reduced body weight on d 21 (P < 0.05). The addition of tannic acid resulted in significant glomerular and glandular hyperplasia, as well as muscularis thickening of the gizzard mucosa. However, broilers could not tolerate tannic acid doses of 0.375 % and above. Tannic acid supplementation may protect the proventriculus mucosal layer, reduce villi atrophy, and enhance growth performance by positively influencing the intestinal microbiota, villus morphology, and intestinal barrier function.

Effects of dietary supplementation of myristic acid on jejunal mucosa-associated microbiota, mucosal immunity, and growth performance of nursery pigs.

The objective of this study was to investigate the effects of myristic acid on jejunal mucosal microbiota, mucosal immunity, and growth performance of nursery pigs. Thirty-six pigs (6.6 ± 0.4kg of body weight) were assigned to three treatments (n = 12) for 35 d in three phases: (NC) basal diet; (PC) NC + bacitracin; and (MA) NC + myristic acid compound. Pigs were euthanized to collect jejunal mucosa, jejunal tissues, and ileal digesta. The PC increased (p < 0.05) the relative abundance (RA) of Lactobacillus spp., and Bifidobacterium boum than the NC group. The MA increased (p < 0.05) RA of Bifidobacterium dentium and Megasphaera spp. than the NC group. The PC tended to decrease IL-8 (p = 0.053) and protein carbonyl (p = 0.075) whereas IgG (p = 0.051) and IL-8 (p = 0.090) in jejunal mucosa were decreased by the MA. The PC increased (p < 0.05) the villus height to crypt depth ratio than the NC group. Both bacitracin and myristic acid improved the intestinal health and growth performance of nursery pigs. Effects of bacitracin were rather immediate whereas the effects of myristic acid were obtained after a 3-week feeding.

Impact of calsporin® (Bacillus subtilis C-3102) supplementation on growth performance and intestinal function in geese.

The objective of this study was to preliminarily explore the effects of Calsporin® (Bacillus subtilis C-3102) on the growth performance, intestinal morphology, apparent digestibility, and cecal microbiota of geese. A total of 144 Sanhua geese, aged 35 days, were randomly divided into three groups: a control group, a group receiving a basal diet supplemented with 30 ppm Bacillus subtilis (B. subtilis), and a group receiving a basal diet supplemented with 60 ppm B. subtilis. Each group had six replicates, with eight geese per replicate. The study consisted of a one-week pre-feeding period followed by a four-week experimental period. The results indicated that, compared to the control group, the group supplemented with 60 ppm B. subtilis showed a significant increase in final body weight and the average daily gain (P < 0.05). Both the 30 ppm and 60 ppm B. subtilis groups exhibited significant improvements in the feed/gain (F/G) ratio (P < 0.05). Additionally, supplementation with either 30 ppm or 60 ppm B. subtilis significantly increased the height of the ileum villi (P < 0.05), with a trend towards an increased villus height to crypt depth (VH/CD) ratio (P > 0.05). In terms of digestive enzyme activity, both 30 ppm and 60 ppm B. subtilis supplementation significantly enhanced the activities of ileum cellulase and chymotrypsin (P < 0.05). Furthermore, the apparent digestibility of crude ash, crude protein, and Ca was significantly improved in the 60 ppm B. subtilis group (P < 0.05). Microbial analysis revealed that B. subtilis increased the abundance of potential probiotics bacterial families, such as Turicibacter, and butyrate-producing, including Prevotellaceae Ga6Al. In conclusion, supplementation with 60 ppm B. subtilis can significantly enhance the growth performance, intestinal morphology, and apparent digestibility of geese. Increasing the supplementation level may further optimize these benefits.

Effects of drinking water supplemented with apple vinegar, essential oils, or colistin sulfate on growth performance, blood lipids, antioxidant status, intestinal morphology, and gut microflora of broiler chickens.

With rising concerns about antibiotic resistance and its consequences on public health, the identification of safe and effective alternatives to antibiotics in the poultry industry has become increasingly critical. This study aimed to evaluate the effects of supplementing drinking water with apple vinegar and essential oils, compared to an antibiotic growth promoter, on the growth performance, serum lipid profile, antioxidant status, intestinal morphology, and gastrointestinal microflora population of broiler chickens. A total of 240 one-day-old male broiler chickens were randomly assigned to four treatments, each consisting of six replicate pens with ten birds per pen. The broiler chickens received the following experimental treatments: normal drinking water without additives (control) and drinking water supplemented with antibiotic (0.25 ml/L), apple vinegar (1 ml/L), and essential oils blend (0.5 ml/L). The results showed that adding antibiotic or essential oils blend to drinking water improved body weight gain and feed conversion ratio throughout the entire rearing period (P < 0.05). The serum levels of cholesterol and low-density lipoprotein in broilers that received apple vinegar or essential oils blend were lower than those in the control and antibiotic groups (P < 0.05). The essential oils blend treatment significantly increased the activity of glutathione peroxidase and superoxide dismutase in the serum of broiler chickens compared to the other treatments (P < 0.05). Furthermore, adding essential oils blend to the drinking water increased villus height and villus height to crypt depth ratio in the jejunum of broilers (P < 0.05). The broiler chickens in the apple vinegar and essential oil blend groups also exhibited a lower population of Enterobacteriaceae and a higher count of lactic acid bacteria in the ceca (P < 0.05). In summary, the addition of essential oils blend to the drinking water of broiler chickens has beneficial effects on production performance and can serve as an effective alternative to antibiotic growth promoters.

Efficacy of advanced chelate technology-based 7-mineral supplementation in mitigating aflatoxin B1-induced impairments in broiler chicken performance and intestinal health.

Optimal levels and bioavailability of trace minerals (TM) in the broiler diet are important for improving performance and health status in the presence of dietary toxins. The study examines the effectiveness of advanced chelate technology-based 7-minerals (ACTM) in broilers fed aflatoxin B1 (AFB1)-contaminated diets, involving 768 chickens in eight treatments with six replicates, following a completely randomized design. Treatments contained (1) negative control (NC) group receiving a basal diet without AFB1 and containing recommended inorganic TM (ITM) levels (NC+ITM), (2) positive control (PC) group receiving a basal diet with 0.5mg AFB1/kg and recommended ITM levels (PC+ITM), (3) PC diet+toxin binder (ITM+TB), (4 and 5) PC diet with 50% and 100% ACTM instead of ITM (ACTM50 and ACTM100), (6 and 7) PC diet with 12.5% and 25% extra ACTM (ITM+ACTM12.5 and ITM+ACTM25), and (8) PC diet with 125% ITM levels (ITM125). The results showed that the ACTM100 and ITM+ACTM25 treatments resulted in higher average weight gain and European production efficiency index compared to the PC+ITM treatment, but lower than the NC+ITM treatment. Key indicators of gut health, such as ileal digestibility of crude fat and phosphorus, AMEn value, duodenal villus height to crypt depth ratio, villus surface area, and gene expression of junctional adhesion molecule 2 were significantly improved in the ACTM100, ITM+BT, and NC+ITM groups compared to the PC+ITM group. Additionally, jejunal occludin expression increased in the ACTM100, ITM+ACTM25, ITM+TB treatments, and the jejunal zonula occludens-1 expression increased significantly in the ACTM100 and ITM+ACTM25 groups. The results indicate that completely replacing ITM with ACTM or adding ACTM supplement to ITM diets at 25% extra commercial levels can improve growth performance, gut health, and nutrient digestibility in the presence of AFB1 challenge. These effects are comparable to diets containing a commercial toxin binder.

Evaluation of the Effect of Bacteriophages and Organic Acids as a Feed Additive to Reduce Salmonella enteritidis in Challenged Chickens.

This study aimed to compare the effects of dietary supplementation of bacteriophage (BP) and acidifiers on performance, meat quality, morphology, and intestinal microbiota in chickens challenged and unchallenged with Salmonella enteritidis (SE) and also to investigate the possibility of replacing them in the diet with antibiotics. A total of 1760 male Ross (308) chicks were randomly assigned to 11 dietary treatments (8 pens/with 20 male chickens in each). Dietary treatments were as follows: SE-uninfected (negative control (NC), a basal diet without supplemention; NC+ 500 g/t BP (NBP1); NC+ 1000 g/t BP (NBP2); NC+ 300 mg/kg acidifier A (NAA); NC+ 300 mg/kg acidifier B (NAB)) and SE-infected (positive control (PC), a basal diet without supplemention; PC+ 40 mg/kg Antibiotic enrofloxacin (PA); PC+ 500 g/t BP (PBP1); PC+ 1000 g/t BP (PBP2); PC+ 3000 mg/kg acidifier A (PAA); PC+ 3000 mg/kg acidifier B (PAB)). At 13 D birds in challenged groups were orally gavaged with 1 mL bacterial suspension containing approximately 108 CFU Salmonella enterica. The results indicated that chicks challenged with SE impaired performance so that BWG and FI significantly decreased and FCR increased (p < 0.05). Diets containing a high dose of BP increased BW and improved FCR in challenged and unchallenged chickens. The quality of breast meat showed a decline in oxidation in chickens challenged with SE (p < 0.05). The inclusion of bacteriophage in the diet of chickens (1000 g/t) improved the L*, b*, and oxidation of meat. The use of bacteriophage and acidifier A in the diet significantly increased the Lactobacillus, and LAB count, especially in the challenged groups. The challenge of chickens with SE decreased the villus height and crypt depth in different parts of the small intestine (p < 0.05). The results of the present study suggested that 1000 g/t of BP probably improves chicken performance by increasing beneficial bacteria and decreasing pathogenic bacteria. Also, bacteriophage showed an improvement in the performance reduction of chickens with Salmonella infection. In addition, improved meat quality and beneficial changes in the microbiome and intestinal morphology indicate the effectiveness of BP as an alternative antibiotic growth promoter in broiler diets.

Yeast cell wall polysaccharides accelerate yet in-feed antibiotic delays intestinal development and maturation via modulating gut microbiome in chickens

BackgroundIt is important to promote intestinal development and maturation of chicks for feed digestion and utilization, intestinal health, and disease resistance. This study aimed to investigate the effects of dietary yeast cell wall polysaccharides (YCWP) addition on intestinal development and maturation of chickens and its potential action mechanism.Methods180 one-day-old male Arbor Acres broilers were randomly assigned to three groups containing control (basal diets without any antibiotics or anticoccidial drug), bacitracin methylene disalicylate (BMD)-treated group (50 mg/kg) and YCWP-supplemented group (100 mg/kg).ResultsCompared with control group, in-feed antibiotic BMD continuous administration significantly decreased crypt depth (d 21) and villus height (d 42) along with mucosal maltase activity (d 42) in the ileum (P < 0.05). Also, BMD markedly downregulated gene expression levels of β-catenin, lysozyme, occludin and FABP-2 (d 21) and innate immune related genes CD83 and MHC-I mRNA levels (d 42, P < 0.05), and decreased goblet cell counts in the ileum of chickens (d 21 and d 42, P < 0.05). While, TLR-2, TLR-6 and iNOS mRNA abundances were notably upregulated by BMD treatment (d 42, P < 0.05). Nevertheless, dietary YCWP addition significantly increased the ratio of villus height to crypt depth (d 21), villus surface area (d 21 and d 42), ileal alkaline phosphatase and maltase activities as well as goblet cell (d 21 and d 42) and IgA-producing plasma cell numbers as compared to BMD treatment (d 21, P < 0.05). YCWP addition also upregulated gene expression levels of Lgr5, Wnt/β-catenin signaling pathway related gene (Wnt3, β-catenin, d 21; β-catenin, d 42), intestinal cells proliferation marker Ki-67 and barrier function related genes (occludin, d 21 and d 42, P < 0.05). Moreover, YCWP significantly increased antigen presenting cell marker related genes (MHC-II, d 21; CD83 and MHC-I, d 42), TLR-1, TLR-2 and TLR-6 mRNA levels (d 21, P < 0.05). Cecal microbiome analysis showed that YCWP addition obviously improved cecal microbial composition, as indicated by increasing relative abundance of Fournierella, Psychrobacter and Ruminiclostridium on d 21, and Alistipes and Lactobacillus on d 42, which were positively related with gut development and maturation related indexes (P < 0.05).ConclusionCollectively, YCWP promoted yet antibiotic BMD delayed intestinal morphological and immunological development linked with modulating gut microbiome in chickens.

Effects of dietary supplementation with butyrate glycerides on lipid metabolism, intestinal morphology, and microbiota population in laying hens.

The present study investigated the impact of butyrate glycerides (BG) on lipid metabolism, intestinal morphology, and microbiota of laying hens. Four hundred eighty 54-week-old Hy-line Brown laying hens were randomly selected and divided into five groups. The control group (ND) was fed a basal diet. Meanwhile, the remaining groups were given a basal supplemented with 0.5, 1, 2, and 4 g/kg of the product containing BG and were designated as BG-0.5, BG-1, BG-2, and BG-4 groups, respectively. The findings showed that: (1) BG supplementation significantly decreased (P < 0.001) the blood Glu levels (BG-0.5, BG-1, BG-2, and BG-4) and increased (P < 0.001) the serum HDL-C levels (BG-2, and BG-4). (2) The BG-2 and BG-4 groups showed an increase (P < 0.01) in abdominal lipid HSL activity. (3) The levels of hepatic TC and TG in all BG groups were significantly decreased (P < 0.05). (4) The addition of BG resulted in a significant reduction in the mRNA expression of the liver X receptor alpha (LXRα) (P < 0.05). (5) All BG groups presented a substantial reduction in duodenal crypt depth and a notable increase in the ratio of villus height to crypt depth (V/C) (P < 0.01). Additionally, all BG groups exhibited a significant increase in villus height in the ileum (P < 0.001). (6) Both the BG-1 and BG-4 groups exhibited a significant reduction in the amounts of n-butyric and n-glutaric acids in the cecum contents (P < 0.05). (7) The inclusion of BG did not substantially impact the diversity of cecal microbiota in laying hens. However, it dramatically boosted the proportion of the beneficial bacterium Alistipes (P < 0.05) and reduced the abundance of the harmful bacterium Verrucomicrobiota (P < 0.05). Overall, incorporating BG with glycerol monobutyrate as the diet's primary active component reduces fat accumulation in laying hens' blood and liver. It potentially regulates lipid metabolism via the PPARγ-LXRα-SREBP1c pathway. Additionally, BG has the potential to enhance the structure of the small intestine's mucous membrane and increase the presence of beneficial bacteria. Under the experimental conditions, late-laying hens supplemented with 4 g/kg BG performed best overall.

Effects of a multi-strain probiotic on rabbits productive traits, antioxidant defence, caecal microbiota and short‑chain fatty acids, and intestinal histomorphology.

This paper aimed to evaluate the effects of a multi-strain probiotic as natural feed additives on the productive performance, blood parameters, antioxidant defence, caecal short‑chain fatty acid profile as well as the effectiveness on the intestinal morphology and on the equilibrium modification of caecal microbiota of growing rabbits. Eighty-six-week-old Italian White rabbits were assigned into two dietary groups: the control group was fed without any additive, while the test group received a diet supplemented with a multi-strain probiotic (MS-Prob; Slab51®) at a dosage of 250 mg/kg diet. The feeding trial lasted up to 12 weeks of age. Rabbits MS-Prob diet had significantly higher daily and final body weight recording also the best of feed efficiency compared to control group. Rabbits in MS-Prob group showed improved slaughter weight and carcass dressing yield. No significant effect was found on meat protein, lipids and ash contents. Serum total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL) and triglycerides decreased significantly in rabbits fed the test-diet. In rabbits fed MS-Prob, the activity of glutathione peroxidase (GPx), glutathione S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) increased significantly, whereas the level of thiobarbituric acid-reactive substance (TBARS) decreased significantly. Caecal pH, ammonia-N and total volatile fatty acids (VFAs) were not significantly influenced by dietary treatments. Conversely, VFAs molar proportions were statistically affected by diets, with higher acetic and butyric acid concentrations in caecum of rabbits fed MS-Prob. Feeding of MS-Prob reduced harmful bacterial growth (Escherichia coli, Bacillus spp., Clostridium spp.) while promoting beneficial bacteria (Lactobacillus spp., Bacteroides spp.). Moreover, MS-Prob positively influences rabbit gut histomorphology, showing higher villus height, villus width, and crypt depth. s: This study indicated that MS-Prob (Slab51®) supplementation stimulated the performance of growing rabbits and positively affected blood serum parameters, intestinal morphology, and caecal environment and microbiota.

Effects of stocking densities on growth, organ index, serum biochemistry, gut morphology and microbiota of young ducks in a rice-duck-crayfish coculture system.

The rice-duck-crayfish (RDC) coculture system, an ecologically efficient breeding method, supports ducks' natural habits and enhances duck welfare. However, the optimal stocking density and its influence on duck health in this system remains undetermined. The study examined the effects of stocking densities on growth, organ index, serum biochemistry, gut morphology and microbiota of ducks in RDC system. A total of five hundred and forty 20-day-old Nonghu No. 2 ducks were randomly divided into low-density (8 birds/666.67 m2, LD), medium-density (12 birds/666.67 m2, MD) and high-density (16 birds/666.67 m2, HD) groups, with three replicates in each group, and the symbiosis period was up to 40 days until rice tasselling. There were no significant differences in final body weight, average daily gain, or feed:gain ratio between groups (p>0.05); the liver and spleen indices of ducks in HD group were significantly greater than in LD group (p<0.05); the serum albumin concentration in HD group decreased, whereas creatine kinase activity increased (p<0.05); the ileal crypt depth significantly increased; and the ileal villus height and villus/crypt ratio significantly decreased in ducks in MD and HD groups than in LD group (p<0.05). Additionally, the abundance of cecal Deferribacterota and Spirochaetota increased significantly (p<0.05), while the abundance of Firmicutes decreased significantly (p<0.05) with increasing stocking density. Moreover, the increase in stocking density significantly decreased the abundance of some beneficial bacteria (Faecalibacterium and Fournierella) and increased the abundance of some harmful bacteria (Mucispirillum and Brachyspira) (p<0.05). These preliminary results suggest that moderately high-density breeding doesn't significantly affect duck growth, but increased stocking density led to changes in cecal microbiota and dysbiosis. Reducing stocking density positively affects immune parameters and ileum morphology.However, due to the limited number of total replicates of the study, further research is needed to validate the reliability of the results.

Reduced Glutathione Promoted Growth Performance by Improving the Jejunal Barrier, Antioxidant Function, and Altering Proteomics of Weaned Piglets.

Reduced glutathione (GSH) is a main nonenzymatic antioxidant, but its effects and underlying mechanisms on growth and intestinal health in weaned piglets still require further assessment. A total of 180 weaned piglets were randomly allotted to 5 groups: a basal diet (CON), and a basal diet supplemented with antibiotic chlortetracycline (ABX), 50 (GSH1), 65 (GSH2), or 100 mg/kg GSH (GSH3). Results revealed that dietary GSH1, GSH2, and ABX improved body weight and the average daily gain of weaned piglets, and ABX decreased albumin content but increased aspartate aminotransferase (AST) activity and the ratio of AST to alanine transaminase levels in plasma. GSH2 significantly decreased glucose content but increased the content of triglyceride and cholesterol in the plasma. Both GSH1 and GSH2 improved the jejunal mucosa architecture (villus height, crypt depth, and the ratio of villus height to crypt depth), tight junction protein (ZO-1 and Occludin), and antioxidant capacity (CAT and MDA), and the effects were superior to ABX. Dietary GSH improved the jejunal barrier by probably inhibiting the myosin light chain kinas pathway to up-regulate the transcript expression of tight junction protein (ZO-1 and Occludin) and Mucins. Through the proteomics analysis of the jejunal mucosa using 4D-DIA, the KEGG pathway enrichment analysis showed that differentiated proteins were significantly enriched in redox homeostasis-related pathways such as glutathione metabolism, cytochrome P450, the reactive oxygen species metabolic pathway, the oxidative phosphorylation pathway, and the phosphatidylinositol 3-kinase-serine/threonine kinase pathway in GSH2 vs. CON and in GSH2 vs. ABX. The results of proteomics and qRT-PCR showed that GSH supplementation might dose-dependently promote growth performance and that it alleviated the weaning stress-induced oxidative injury of the jejunal mucosa in piglets by activating SIRTI and Akt pathways to regulate GPX4, HSP70, FoxO1. Therefore, diets supplemented with 50-65 mg/kg GSH can promote the growth of and relieve intestinal oxidative injury in weaned piglets.

Probiotic administration aggravates dextran sulfate sodium salt-induced inflammation and intestinal epithelium disruption in weaned pig

BackgroundA. muciniphila (AKK) has attracted extensive research interest as a potential next-generation probiotics, but its role in intestinal pathology is remains unclear. Herein, this study was conducted to investigate the effects of A. muciniphila DSM 22,959 on growth performance, intestinal barrier function, microecology and inflammatory response of weaned piglets stimulated by dextran sulfate sodium salt (DSS).MethodTwenty-four Duroc × Landrace × Yorkshire (DLY) weaned piglets used for a 2 × 2 factorial arrangement of treatments were divided into four groups with six piglets in each group. From 1 to 15 d, the CA and DA groups were orally fed with 1.0 × 1011 colony-forming units A. muciniphila per day, while the CON and DCON groups were received gastric infusion of anaerobic sterile saline per day. The pigs were orally challenged (DCON, DA) or not (CON, CA) with DSS from day 9 to the end of the experiment and slaughtered on day 16.ResultsPresence of A. muciniphila in DSS-challenged weaned pigs resulted in numerically increased diarrhea rate, blood neutrophilic granulocyte, serum C-reactive protein and immunoglobulin M levels, and numerically reduced final weight, average daily feed intake and average daily gain. The decrease in intestinal villus height, villous height: crypt depth ratio and digestibility was accompanied by lower expression of ZO1, ZO2, Claudin1, DMT1, CAT1, SGLT1 and PBD114 genes, as well as decreased enzyme activities of intestinal alkaline phosphatase, lactase, sucrase and maltase of piglets in DA group compared to piglets in DCON group. The abundance of Bifdobacterium, Lactobacillus, A. muciniphila, Ruminococcus gnavus was numerically higher in digesta of pigs in DA group than those in DCON group. The inflammatory responses of piglets were dramatically changed by the simultaneous presence of A. muciniphila and DSS: expression level of IL17A, IL17F, IL23, RORγt, Stat3 was elevated in DA pigs compared to the other pig groups.ConclusionsOur result showed that the oral A. muciniphila aggravates DSS-induced health damage of weaned piglet, which may attribute to the deteriorating intestinal morphology, dysbiosis of microbiota and inflammatory response disorders.

The Use of Coffee Cherry Pulp Extract as an Alternative to an Antibiotic Growth Promoter in Broiler Diets.

Coffee cherry pulp (CCP) is a by-product of coffee bean production. CCP contains amounts of phenolic compounds that are beneficial for animals. This study evaluated the impact of coffee cherry pulp extract (CCPE) supplementation on growth performance, meat quality, carcass characteristics, serum biochemistry, cecum microbial population, intestinal morphology, and immune and antioxidant responses of broilers. Five hundred 1-day-old Ross 308 chicks were randomly assigned to five groups: a basal diet control, a basal diet with antibiotic growth promoters at 0.25 g/kg, and groups supplemented with CCPE at 0.5, 1.0, and 2.0 g/kg diet over 35 days. The results showed that throughout the experimental period, the groups supplemented with CCPE improved their final weight, average daily gain, and feed conversion ratio (p < 0.05). CCPE at 1.0 and 2.0 g/kg diet reduced the average daily feed intake (p < 0.05). In addition, CCPE at 0.5 g/kg reduced levels of serum alanine transaminase and aspartate aminotransferase (p < 0.05). Triglyceride levels were the lowest in CCPE 2.0 (p < 0.05). In the group supplemented with CCPE at all levels, the high-density lipoprotein levels significantly increased (p < 0.05). Drip loss in the breast at 24 and 48 h decreased (p < 0.05). Additionally, live weight, defeathered weight, and carcass weight significantly increased (p < 0.05). Furthermore, CCPE improved intestinal morphology, especially villus height and the villus height per crypt depth ratio (p < 0.05). CCPE supplementation also reduced pathogenic bacteria, increased Lactobacillus spp. (p < 0.05), and increased the expression of immune-related genes and antioxidant activity in the liver and intestines (p < 0.05). Therefore, the use of CCPE as an alternative to antibiotics in broiler feed improved growth performance and health parameters in broilers. It provides a sustainable and environmentally friendly option for supplementary feed, contributing to more efficient poultry nutrition management.

Long-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA1b in the rat small intestine.

Although long-term high dietary sodium consumption often aggravates hypertension and bone loss, sodium in the intestinal lumen has been known to promote absorption of nutrients and other ions, e.g., glucose and calcium. However, whether high-salt diet (HSD) altered mucosal morphology, villous cell turnover and calcium transporter expression remained elusive. Herein, rats were treated with HSD containing 8% wt/wt NaCl for up to 5 months. HSD rats exhibited a marked increase in sodium intake with high fecal and urinary sodium excretion, as compared to the control group treated with normal diet. Intestinal histomorphometry revealed increasing of crypt depth and villous height in 3- and 4-month HSD groups, respectively, consistent with larger mucosal-to-serosal amplification ratio that reflected an increased surface area for nutrient absorption. The signals of Ki-67-positive cells was enhanced in the crypts as visualized by multiphoton fluorescence microscopy, whereas the TUNEL-positive cells were decreased in the villi of HSD, suggesting greater crypt cell proliferation and a reduction of villous cell apoptosis. Confocal microscopy showed higher expression of TRPV6 protein in the villous tip of HSD, while PMCA1 expression was increased in villous tip and crypt areas. The percentage of cells with highly expressed uroguanylin-an endogenous intestinal natriuretic peptide-was significantly higher in HSD group. In conclusion, HSD profoundly changed the intestinal morphology and turnover of epithelial cell, increased the expression of calcium transporters and uroguanylin. Our findings reflect pathophysiological adaptations in the intestine, which might be another target organ for drug discovery against HSD-induced osteopathy in the future.

Development of an ex vivo model to assess the impact of fumonisin B1 on swine intestinal morphology.

This study was conducted to assess the effects of fumonisin B1 (FB1) on the jejunum of pigs using a novel ex vivo model conducted in parallel with an in vivo trial. For the in vivo model, twelve male 28 to 70-days-old pigs were subjected to two treatments of six animals each: the control group, fed a basal diet (BD), and the FB1 group, fed the BD + 50 mg/kg FB1. At 70 days, the animals were slaughtered and one jejunal sample was collected from each pig for further histopathological analyses. Other four male pigs from the in vivo control treatment were slaughtered at 70 days for the ex vivo model. Four jejunal explants were collected from each pig, totaling 16 intestinal explants, which were subjected to two treatments, with 8 explants each, using an Ussing Chamber (UC) system: the control group, subjected to buffer solution (BS), and the FB1 group, subjected to BS + 50 mg/L FB1. Samples from in vivo and ex vivo models were analyzed for histopathological parameters and subjective intestinal assessments. The FB1 group presented lower (P < 0.05) villi height than the control group in both in vivo and ex vivo. A decrease (P < 0.05) in the villi number, crypt depth, enterocyte height and enterocyte nucleus size was also observed in the FB1 group ex vivo, with a higher severity score of lymphatic vessels dilation than the control (P = 0.0459). The FB1 group also tended to increase the goblet cells count (P = 0.0736) ex vivo as well as to decrease the crypt width (P = 0.0638) in vivo. The ex vivo model exhibited similar mean values and statistical responses to those observed in vivo, demonstrating its potential as an alternative approach for assessing the effects of mycotoxins in a reduced number of animals.