We examine the partial melting and the cooling history of a ~5 km section of mantle lithosphere preserved in the Donjek massif, part of a Permian ophiolite in the northern Cordillera of Yukon, Canada. The mantle rocks are depleted spinel harzburgite containing <3% clinopyroxene displaying steep rare-earth element (REE) chondrite-normalized profiles and low (Gd/Yb)n (0.02 to 0.07) compared to most other ophiolites. The REE patterns of clinopyroxene can be modeled as 16–20% partial melts of typical depleted mid-ocean ridge (MOR) mantle. The REE exchange between coexisting ortho- and clinopyroxene preserves temperatures (TREE) of 1150–1360 °C, some of the highest values recorded in ophiolites and abyssal peridotites, and show a positive correlation with CaMg exchange (solvus) temperatures (TBKN) of 900–970 °C. The harzburgite represents lithosphere formed at an initial melting temperature of ~ 1350 °C that cooled at rate of 10−1 to 10−4 °C/year as deduced by TREE values with cation diffusion and grain size data. The TREE temperatures and cooling rates for the Donjek massif show a regular systematic variation with depth from the crust-mantle transition along a trend similar to the Samail ophiolite of Oman, consistent with conductive heat transfer beneath a cool lower crust. High near-solidus temperatures and the cooling rates in the massif were a consequence of rapid obduction against oceanic crust along either a transform or low angle detachment soon after melt extraction. Final emplacement of the ophiolite as klippen on underlying continental crust occurred ~ 40 m.y. later.