Objectives Karyomapping was developed as a means of screening for monogenic and chromosomal disorders simultaneously in human IVF embryos using SNP chips. SNP chips in cattle breeding on the other hand are used for genomic selection i.e. to establish estimated breeding values (e.g. for food conversion efficiencies, milk production etc.). This has led to significantly improved rates of genetic gain through the screening and selection of animals at birth, but with the inherent drawback of having to wait for calf to be born before the result can be obtained and the fact that improvements within a herd breed are limited by the number of animals born. Application of genomic selection to in vitro produced (IVP) embryos however could bring further benefits thanks to the ability to test a greater number of individuals in a shorter time, thereby increasing selection speed and intensity. Aneuploidy is common in IVP embryos and could lead to developmental arrest. Karyomapping can therefore allow simultaneous genomic selection and aneuploidy detection, offering the power to maximise pregnancy rates of high quality offspring. Moreover, Karyomapping can be used to characterise the frequency and parental origin of aneuploidy. The objective of this study therefore was to apply Karyomapping to cattle breeding with a view to establishing the first live births by this approach. Method We used Karyomapping to characterise the frequency and parental origin of aneuploidy in IVP bovine embryos in order to establish an estimate of total aneuploidy rates in each parental germline. Furthermore, estimations of genome wide recombination rates in cattle were obtained and we performed the first embryo transfers. Results Following Karyomapping of trophectoderm biopsies, 68.8% of the embryos (n=42/61) appeared euploid while the rest displayed one or more abnormalities. Trisomies were detected with a frequency of 2.10% (n=68/3248 chromosomes) while monosomies with a frequency of just 0.31% (n=10/3248 chromosomes) resulting in a statistically significant difference. Monosomies appeared with a similar frequency of 0.24% (n=4/1653) and 0.36% (n=6/1653) in chromosomes of paternal and maternal origin, respectively. However, trisomies appeared with a frequency of 2.60% (n=43/1653) in chromosomes of maternal origin while the frequency for paternal chromosomes was 1.51% (n=25/1653) resulting in a significant difference. The average genome-wide recombination distance per Mb was calculated as 1.67 cM/Mb, which is higher than previous studies. Moreover, the maternal X chromosome showed a much-reduced number of crossovers than other chromosomes of similar size. Following transfer of euploid embryos, 4 live births are reported at time of writing. Conclusions We demonstrate, for the first time, a proof of principle for the application of Karyomapping to cattle breeding, with the birth of four calves after analysis in this way. This combined genomic selection and PGT-A approach was highly reliable, with calves showing 98% concordance with their respective embryo biopsies for SNP typing and 100% concordance with their respective biopsies for aneuploidy screening. This approach permits a significant increase in the rate of genetic gain and is applicable to basic research to investigate meiosis and aneuploidy in cattle.