Crosstalk Removal Research Articles

Mosaic-PICASSO: accurate crosstalk removal for multiplex fluorescence imaging.

Ultra-multiplexed fluorescence imaging has revolutionized our understanding of biological systems, enabling the simultaneous visualization and quantification of multiple targets within biological specimens. A recent breakthrough in this field is PICASSO, a mutual-information-based technique capable of demixing up to 15 fluorophores without their spectra, thereby significantly simplifying the application of ultra-multiplexed fluorescence imaging. However, this study has identified a limitation of mutual information (MI)-based techniques. They do not differentiate between spatial colocalization and spectral mixing. Consequently, MI-based demixing may incorrectly interpret spatially co-localized targets as non-colocalized, leading to overcorrection. We found that selecting regions within a multiplex image with low-spatial similarity for measuring spectroscopic mixing results in more accurate demixing. This method effectively minimizes overcorrections and promises to accelerate the broader adoption of ultra-multiplex imaging. The codes are available at https://github.com/xing-lab-pitt/mosaic-picasso.

Deep Learning for Enhancing Multisource Reverse Time Migration

Reverse time migration (RTM) is a technique used to obtain high-resolution images of underground reflectors; however, this method is computationally intensive when dealing with large amounts of seismic data. Multi-source RTM can significantly reduce the computational cost by processing multiple shots simultaneously. However, multi-source-based methods frequently result in crosstalk artifacts in the migrated images, causing serious interference in the imaging signals. Plane-wave migration, as a mainstream multi-source method, can yield migrated images with plane waves in different angles by implementing phase encoding of the source and receiver wavefields; however, this method frequently requires a trade-off between computational efficiency and imaging quality. We propose a method based on deep learning for removing crosstalk artifacts and enhancing the image quality of plane-wave migration images. We designed a convolutional neural network that accepts an input of seven plane-wave images at different angles and outputs a clear and enhanced image. We built over 500 1024×256 velocity models, and employed each of them using plane-wave migration to produce raw images at 0°, ±10°, ±20°, and ±30° as input of the network. Labels are high-resolution images computed from the corresponding reflectivity models by convolving with a Ricker wavelet. Random sub-images with a size of 512×128 were used for training the network. Numerical examples demonstrated the effectiveness of the trained network in crosstalk removal and imaging enhancement. The proposed method is superior to both the conventional RTM and plane-wave RTM (PWRTM) in imaging resolution. Moreover, the proposed method requires only seven migrations, significantly improving the computational efficiency. In the numerical examples, the processing time required by our method was approximately 1.6% and 10% of that required by RTM and PWRTM, respectively.

A Study on Correcting the Effect of Polarization Crosstalk in Full-Disk Solar Photospheric Magnetic Field Observations

Magnetography using magnetic sensitive lines is regarded traditionally as the main instrument for measuring the magnetic field of the whole Sun. Full polarized Stokes parameters (\(I\), \(Q\), \(U\), \(V\)) can be used to deduce the magnetic field under specific theoretical models or inversion algorithms. Due to various reasons, there are often cross-talk effects among Stokes signals observed directly by magnetographs. Especially, the circularly polarized signal \(V\) usually seriously affects the linearly polarized \(Q\) and \(U\) signals, which is one of the main errors in the value of the transverse magnetic field (parallel to the solar surface) that is related to \(Q\) and \(U\). The full-disk magnetograph onboard the Advanced Space based Solar Observatory (ASO-S/FMG) is designed to observe Stokes parameters and deduce the magnetic-field vector. In this article, the methods for correcting the effects of cross-talk \(V\) on \(Q\) and \(U\) based on the assumption of perfectly symmetric \(Q\) and \(U\) and anti-symmetric Stokes \(V\) profiles and a new method for reducing the crosstalk effect under observation mode of the FMG are developed. Through the test, it is found that the two methods are effective in cross-talk removal in the sunspot region and have better consistency. Additionally, the developed method can be applied to remove the cross-talk effect by using only one group of \(Q\), \(U\) and \(V\) images observed at one wavelength position.

Crosstalk Removal in Forward Scan Sonar Image Using Deep Learning for Object Detection

This paper proposes the detection and removal of crosstalk noise using a convolutional neural network in the images of forward scan sonar. Because crosstalk noise occurs near an underwater object and distorts the shape of the object, underwater object detection is limited. The proposed method can detect crosstalk noise using the neural network and remove crosstalk noise based on the detection result. Thus, the proposed method can be applied to other sonar-image-based algorithms and enhance the reliability of those algorithms. We applied the proposed method to a three-dimensional point cloud generation and generated a more accurate point cloud. We verified the performance of the proposed method by performing multiple indoor and field experiments.

Correcting for color crosstalk and chromatic aberration in multicolor particle shadow velocimetry

Color crosstalk and chromatic aberration can bias estimates of fluid velocity measured by color particle shadow velocimetry (CPSV), using multicolor illumination and a color camera. This article describes corrections to remove these bias errors, and their evaluation. Color crosstalk removal is demonstrated with linear unmixing. It is also shown that chromatic aberrations may be removed using either scale calibration, or by processing an image illuminated by all colors simultaneously. CPSV measurements of a fully developed turbulent pipe flow of glycerin were conducted. Corrected velocity statistics from these measurements were compared to both single-color PSV and LDV measurements and showed excellent agreement to fourth-order, to well into the viscous sublayer. Recommendations for practical assessment and correction of color aberration and color crosstalk are discussed.

Dual-polarization multi-band optical OFDM transmission and transceiver limitations for up to 500 Gb/s uncompensated long-haul links.

A number of critical issues for dual-polarization single- and multi-band optical orthogonal-frequency division multiplexing (DP-SB/MB-OFDM) signals are analyzed in dispersion compensation fiber (DCF)-free long-haul links. For the first time, different DP crosstalk removal techniques are compared, the maximum transmission-reach is investigated, and the impact of subcarrier number and high-level modulation formats are explored thoroughly. It is shown, for a bit-error-rate (BER) of 10(-3), 2000 km of quaternary phase-shift keying (QPSK) DP-MB-OFDM transmission is feasible. At high launched optical powers (LOP), maximum-likelihood decoding can extend the LOP of 40 Gb/s QPSK DP-SB-OFDM at 2000 km by 1.5 dB compared to zero-forcing. For a 100 Gb/s DP-MB-OFDM system, a high number of subcarriers contribute to improved BER but at the cost of digital signal processing computational complexity, whilst by adapting the cyclic prefix length the BER can be improved for a low number of subcarriers. In addition, when 16-quadrature amplitude modulation (16QAM) is employed the digital-to-analogue/analogue-to-digital converter (DAC/ADC) bandwidth is relaxed with a degraded BER; while the 'circular' 8QAM is slightly superior to its 'rectangular' form. Finally, the transmission of wavelength-division multiplexing DP-MB-OFDM and single-carrier DP-QPSK is experimentally compared for up to 500 Gb/s showing great potential and similar performance at 1000 km DCF-free G.652 line.

Development of Movement Crosstalk Removal Method for 3D Elliptical Vibration Cutting

The three-dimensional elliptical vibration cutting can realize high efficient and low cost of method of optical free surface cutting, whose effective drive form is the stack of piezoelectric ceramics to achieve the vibration in three directions . but the crosstalk phenomenon generated in the process of the piezoelectric movement make the tool deviate the ideal trajectory so that seriously affect the quality of the workpiece surface, damage the tool. Thus a method of removing the crosstalk generated in the three-dimensional elliptical vibration cutting device and its control system is proposed in the paper. The control system model of a three-dimensional elliptical vibration cutting is established, then, the crosstalk data is assumed, using the least squares method to fit the date, crosstalk compensation algorithm is obtained so that the crosstalk removal module can be designed to remove the crosstalk generated the piezoelectric movement ,the final section is the simulation-based analysis, the simulation results show that the method can meet the control requirements of the three-dimensional elliptical vibration, the crosstalk of piezoelectric movement can be removed effectively.

Joint Color Decrosstalk and Demosaicking for CFA Cameras

In interest of low cost, low power consumption, and compact size, most digital cameras adopt a design of single sensor array coupled with a color filter array. This design inevitably suffers, due to physical characteristics of the optical and semiconductor components and the imperfection of manufacturing, from the problem of crosstalk between different color channels. Channel crosstalk can desaturate colors and blur image details, but the problem was seemingly overlooked by existing color demosaicking algorithms. To rectify this deficiency we propose a new joint demosaicking and decrosstalk technique that counters the effects of channel crosstalks by adaptive least-squares inverse filtering. The new technique integrates the operations of deconvolution for crosstalk removal and interpolation for color demosaicking, and it introduces a general framework in which any spatially varying crosstalks and varying spatial-spectral correlations can be modeled and factored into the color reproduction. Simulation results show that the proposed technique is highly effective and capable to obtain both high color fidelity and sharp, clean spatial details.

Calibration of linearly polarized polarimetric SAR data subject to Faraday rotation

A model for linearly polarized fully polarimetric backscatter measurements is used, incorporating the effects of system noise, channel amplitude, phase imbalance, crosstalk, and Faraday rotation. A step-by-step procedure is outlined for correction (or calibration) of fully polarimetric data subject to Faraday rotation, to recover the true scattering matrix. The procedure identifies steps for crosstalk removal and correction of channel imbalances that are robust in the presence of Faraday rotation. The final steps in the procedure involve a novel strategy for estimation and correction of Faraday rotation. Three approaches to estimate the (one-way) Faraday rotation angle /spl Omega/ directly from linear (quad-) polarized synthetic aperture radar (SAR) backscatter data obtained by a spaceborne SAR system are described. Each approach can initially be applied to the signature of any scatterer within the scene. Sensitivity analyses are presented that show that at least one of the measures can be used to estimate /spl Omega/ to within /spl plusmn/3/spl deg/ to 5/spl deg/, with reasonable levels of residual crosstalk, noise floor, channel amplitude, and phase imbalance. Ambiguities may be present in the estimates of /spl Omega/ of /spl plusmn/n/spl pi//2 - the impact of this is discussed, and several approaches are suggested to deal with this possibility. The approach described in this paper is relevant for future L-band spaceborne SARs and removes one key obstacle to the deployment of even longer wavelength SARs (e.g., an ultrahigh frequency or P-band SAR) in Earth orbit.

A nonlinear recursive least squares algorithm for crosstalk‐resistant noise canceler

AbstractIn this paper, a method of updating filter coefficients is proposed in which the RLS (Recursive Least Squares) algorithm is applied to a noise canceler with a filter for crosstalk removal. The RLS algorithm has an advantage over the Least Mean Square algorithm by virtue of better convergence. When the filter coefficients are updated including the filter for crosstalk removal, the error signal becomes nonlinear in terms of filter coefficients. Hence, a nonlinear RLS algorithm is derived in which such nonlinearity is taken into account. It is shown that the convergence is further improved in comparison with the conventional RLS algorithm. Finally, a method is proposed to reduce the noise contained in the transmitted voice signals of telephone by means of the present noise canceler and its effectiveness is evaluated. © 2003 Wiley Periodicals, Inc. Electron Comm Jpn Pt 3, 86(5): 36–44, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecjc.10039

Cross-talk filtering in four dye fluorescence-based DNA sequencing.

We have addressed two important issues of nonlinear cross-talk and baseline adjustment in DNA data processing. An important aspect in the processing of the four-dye fluorescence-based data is the cross-talk filtering. Typically, a matrix M, which is a function of the fluorophores and the fluorescence detection system, is used in the multicomponent analysis. In this deconvolution process the matrix is applied directly to the raw signal, on a linear cross-talk assumption. This necessitates the signal to be aligned to the baseline before the filter is applied. The various techniques used for aligning the raw data have the negative effect of adding distortion to the signal. An algorithm for cross-talk removal is presented in this paper. The algorithm uses the intensity difference of the signal rather than the actual value itself, thus making the cross-talk removal possible before the base line adjustment. In addition, a supplementary filtering step is proposed in order to account for the nonlinear nature of the cross-talk. This second step is based on a matrix T that accounts for the correlation of each of the signals with the other three. The overall result is a more precise presentation of the DNA data and less information loss through filtering.

A unified algorithm for phase and cross-talk calibration of polarimetric data-theory and observations

A unified approach to phase and cross-talk calibration of polarimetric data which can be applied to calibrating scattering matrix data or to extraction of the descriptors of distributed targets is described. It relies on the scene being dominated by targets with uncorrelated like and cross-polarized backscattering coefficients, but provides cross-talk calibration of targets for which this is not true. The algorithm needs unsymmetrized data, but uses only quantities derived from the covariance matrix of large areas. It makes no assumptions about system reciprocity, permits ready interpretation of the terms in the calibration procedure, allows comparison of the relative magnitude of the system-induced mixing of terms in the observed covariance matrix, is noniterative, and produces indicators which allow testing of whether it meets its own underlying assumptions. The linear distortion model is shown to lead to an inconsistent system of equations; this inconsistency can be removed by introducing an extra parameter which has properties expected of system noise. The modulus of the copolarized correlation coefficient, which is important in polarimetric classification and as a phase descriptor, is shown to be invariant under all effects embodied in the linear distortion model. Calibration of the scattering matrix data is based on a minimum least squares principle. This suggests that current methods of symmetrization are not optimal. The same analysis shows that estimates of parameters needed to form an equivalent reciprocal system are also nonoptimal. The method is more general than the well-known van Zyl algorithm for cross-talk removal, and permits an analysis of the conditions under which the van Zyl algorithm will yield valid results. Correction of phase distortion induced by channel imbalance Is treated as an optional extra step relying on a known HH-VV phase difference in some region of the image. Results from the algorithm are discussed using scattering matrix data from the 1989 MAESTRO campaign. >

Symmetrization of cross-polarized responses in polarimetric radar images using reciprocity

A new method for symmetrizing polarimetric scattering matrices is applied to the polarimetric synthetic aperture radar (SAR) images acquired over Mount Shasta. This method symmetrizes the cross-polarized responses in the polarimetric images using a 2*2 matrix derived from the image itself and based on the reciprocal property of natural distributed targets. The covariance parameters of the in-scene trihedral reflectors are presented to demonstrate the effectiveness of this method. The results are also compared with those obtained by the symmetrization technique employed by POLCAL, before and after crosstalk removal. Before crosstalk is removed from the images, there were no significant differences between the results obtained by the POLCAL method and the new method for the covariance parameters of trihedral reflectors. After crosstalk removal, using distributed targets with reflection symmetry, the new symmetrization method outperformed the POLCAL method.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>