Cross-species Protection Research Articles

Eimeria tenella and E. acervulina: Differences in Ability to Elicit Cross-Species Protection as Compared with the Turkey Coccidium, E. adenoeides

Repeated oral inoculation of turkey poults with large doses (1 x 10(6) oocysts) of the chicken coccidia, Eimeria tenella or E. acervulina, failed to prevent weight loss, poor feed conversion, and intestinal pathology in turkeys challenged with the turkey coccidium, E. adenoeides. Invasion by E. tenella in turkeys was significantly greater than invasion by E. adenoeides in chickens; by 24 hr postinoculation (PI), the numbers of E. tenella and E. adenoeides sporozoites in the ceca had decreased markedly as compared with the numbers that initially invaded, and they did not differ significantly from each other. At 24 hr PI, however, transfer of cecal scrapings from chickens or turkeys inoculated with E. adenoeides produced infection in 53% of the recipient turkeys, but transfer of scrapings from either chickens or turkeys inoculated with E. tenella failed to produce infection in 20 attempts with recipient chickens. Cultured chicken peripheral blood monocytes (PBMs) that were inoculated with E. adenoeides sporozoites contained numerous vesicles that were recognized by the refractile body-specific monoclonal antibody 1209; the number of vesicles was markedly decreased in PBM cultures inoculated with gamma-irradiated E. adenoeides sporozoites. Very few vesicles were detected in the cytoplasm of turkey PBMs that contained E. tenella sporozoites, and none were detected in turkey PBMs containing E. adenoeides sporozoites. The survival of infective sporozoites, along with the secretion of refractile body antigen, may be more critical to the development of cross-species immunity than the number of sporozoites that initially invade the foreign host.

Leishmania pifanoi amastigote antigens protect mice against cutaneous leishmaniasis.

In the search for a leishmaniasis vaccine, extensive studies have been carried out with promastigote (insect stage) molecules. Information in this regard on amastigote (mammalian host stage) molecules is limited. To investigate host immune responses to Leishmania amastigote antigens, we purified three stage-specific antigens (A2, P4, and P8) from in vitro-cultivated amastigotes of Leishmania pifanoi by using immunoaffinity chromatography. We found that with Corynebacterium parvum as an adjuvant, three intraperitoneal injections of 5 micrograms of P4 or P8 antigen provided partial to complete protection of BALB/c mice challenged with 10(5) to 10(7) L. pifanoi promastigotes. These immunized mice developed significantly smaller or no lesions and exhibited a 39- to 1.6 x 10(5)-fold reduction of lesion parasite burden after 15 to 20 weeks of infection. In addition, P8 immunization resulted in complete protection against L. amazonensis infection of CBA/J mice and partial protection of BALB/c mice, suggesting that this antigen provided cross-species protection of mice with different H-2 haplotypes. At different stages during infection, vaccinated mice exhibited profound proliferative responses to parasite antigens and increased levels of gamma interferon production, suggesting that a Th1 cell-mediated immune response is associated with the resistance in these mice. Taken together, the data in this report indicate the vaccine potential of amastigote-derived antigens.

Eimeria maxima gametocyte antigens: potential use in a subunit maternal vaccine against coccidiosis in chickens

Affinity-purified gametocyte antigens (APGA) from Eimeria maxima, emulsified in Freund's adjuvant, were injected intramuscularly into breeding hens on two or three occasions. As a result, progeny of the immunized hens were partially immune to infection with E. maxima, Eimeria tenella and Eimeria acervulina (with a reduction in total oocyst output of 45–63% as compared with progeny of untreated hens). Thus, APGA in Freund's adjuvant appears to have great potential as part of a maternally applied vaccine against coccidiosis. The ability of APGA to induce partial cross-species protection is most probably due to the existence of conserved epitopes in the different species as indicated by comparative Western blots of E. maxima and E. tenella. Surprisingly, Freund's adjuvant by itself also induced significant levels of maternal immunity to coccidiosis (with a 12–35% reduction in oocyst output in the progeny). In contrast to the purified antigens or Freund's alone, crude extracts from gametocytes as well as other developmental stages, induced little if any significant maternal immunity despite provoking the production of large amounts of parasite-specific IgG, including antibodies to APGA. This result indicates that a successful maternal vaccine against coccidiosis requires, in addition to good recognition of protective antigens, the exclusion of irrelevant antigens from the vaccine preparation.

Vaccination against gastrointestinal nematodes of sheep using purified secretory acetylcholinesterase from Trichostrongylus colubriformis--an initial pilot study.

Purified secretory acetylcholinesterase (sAChE) from Trichostrongylus colubriformis was used as a candidate vaccine against mixed T. colubriformis, Haemonchus contortus and C. oncophora challenge infections of sheep. Cross species protection was achieved with an average reduction in worm burden of all species of 31%, rising to 58% in individual cases. There was no consistent reduction in faecal egg counts and increases in anti-T. colubriformis sAChE IgG antibody levels following vaccination were modest. We suggest that improved antigen delivery systems will result in increased host protection.

Cell-mediated immunity to Eimeria in the fowl: The absence of cross-species protection is not due to the lack of cross-reactive T cells

Immunity to Eimeria species in the fowl has been shown to be species specific and it has been proposed that the lack of cross-protection between the species of Eimeria is due to the absence of T cells which recognize antigens from a heterologous species. When this hypothesis was tested the results showed that antigens from E. tenella elicited a strong specific response by cells from birds immune to E. tenella. In contrast cells from birds which were immune to E. acervulina responded to a similar magnitude to both E. tenella and E. acervulina antigens. This indicates that the lack of cross-protection is not due to the lack of cross-reactive T cells.

Interferon Production in Rat Type II Pneumocytes and Alveolar Macrophages

The time course and magnitude of interferon production induced by influenza virus were determined in type II pneumocytes and alveolar macrophages isolated from rats (Sprague-Dawley). Although the peak of interferon production was approximately 20 h in both alveolar cell types, it was more than three- to fourfold higher in type II cells than in macrophages. Dose-response relationships were noted between the virus multiplicity of induction as well as population numbers of either alveolar cell type and interferon yields. The viral-induced cytokines produced by rat type II cells and alveolar macrophages exhibit physiochemical and biological properties characteristic of interferons and, with respect to type II pneumocytes, mark their heretofore unrecognized capability to produce interferon. The best cross-species antiviral protection afforded by these rat interferons to cells of different origin, expressed as percentage of homologous species (100%), was as follows: guinea pig 50, mouse 40%, and both human and monkey 0%. The heterologous antiviral activity by interferons from either rat alveolar macrophages or type II cells on alpha interferon-sensitive guinea pig cells suggests that these cytokines may be more appreciably related to the alpha-like interferon species. The growth of influenza and Sendai viruses was precluded in both rat alveolar macrophages and type II pneumocytes. The findings herein suggest that type II cells may be a major source of alveolar interferon for activating the antiviral state and modulating alveolar cell functions requisite for lung integrity.

Properties of bovine interferons.

This study was done in an attempt to elucidate some of the properties of bovine IFNs. Maximum levels of both fibroblast and leukocyte IFNs occurred prior to 24 h whereas maximum levels of immune IFN were not reached until after 72 h. The latter species of IFN was unstable at either pH 2 or 56 degrees C whereas both the fibroblast and leukocyte IFNs were more stable under these conditions. Studies of cross-species protection between fibroblast and leukocyte IFNs indicate that the former was more protective for other species than the latter.

Neutralization of interferon activity in homologous and heterologous cells with homologous and heterologous antibody.

AbstractHuman fibroblast interferon (HFIF) and human leukocyte interferon (HLIF) can protect mouse L-929 cells against the cytopathic effects of vesicular stomatitis virus (VSV), although HLIF is about 60 times and HFIF about 1200 times less active than mouse interferon (MIF). This relationship suggests that, with repsect to the mouse L-929 receptor, there exists a greater similarity between the active sites of MIF and HLIF than between MIF and HFIF. Employing antibodies directed against MIF, HFIF, and HLIF as a probe, we found interferon-cell interactions to be stronger for homologous than for heterologous combinations. A variable-fit model is proposed to explain the differences that interferons display in cross-species protection. Thus, the closer the fit between interferon and receptor, the greater the antiviral response.