Degradation of macroporous resin of TDI cross-linked cellulose named TCC prepared by crosslinking cellulose with TDI was investigated. The life span for 70 % weight loss, which was estimated based on the changes in dynamic parameters obtained from thermogravimetric analysis, was 74,507 h. Three bacteria including Trametes gallic, Gloeophyllum trabeum, Aspergillus, which were separated and purified from nature, were used to decompose macroporous resin solely or in combination. The life span was reduced to 720 h when combination of Trametes gallic, Gloeophyllum trabeum, Aspergillus was used to decompose TCC at 25 °C, relative humidity of 70 % and pH = 5. Also, the influence of carbon content, nitrogen content and pH value on the biodegradation rate of TCC was discussed. Degradation rate of TCC was highest when mass ratio of glucose to TCC was 0.04, mass ratio of nitrogen to TCC was 0.04 and pH value was 5.