Cross-linked Microspheres Research Articles

Droplet Microfluidic Method for Estimating the Dynamic Interfacial Tension of Ion-Crosslinked Sodium Alginate Microspheres.

The research focuses on optimizing the production of hydrogel microspheres using droplet microfluidics for pharmaceutical and bioengineering applications. A semiempirical method has been developed to predict the dynamic interfacial tension at the interface of ion-cross-linked sodium alginate microsphere-sunflower oil modified with glacial acetic acid and Tween 80 surfactant. These microspheres are produced in a small-scale coaxial device that is manufactured using affordable DLP/LCD 3D printing technology with a transparent photopolymer. The method was tested to design the minireactor in the device, which allows for the production of fully cross-linked microspheres that are ready for use at the output of the reactor without additional cross-linking steps in the microsphere collector. The mathematical expression for estimating the interfacial tension at the moment of formation of a hydrogel microsphere includes the Reynolds number for a two-phase liquid, the Ohnesorge number, and the surface tension at the liquid-air interface for continuous medium flow (modified oil). The reliability of the prediction is confirmed for continuous medium and dispersed phase flow rates of 0.8-3.2 and 0.01-0.08 mL/min, respectively. The evolution of the interfacial tension from the moment the microspheres formed and the estimated ultimate interfacial tension in a cross-linked hydrogel-modified oil system contributed to the reliable determination of the linear size of a minireactor. The ultimate interfacial tension of 76.5 ± 0.3 mN/m was determined using the Young-Laplace equation, which is based on measuring the surface free energy of the hydrogel as soft matter using the Owens-Wendt method. Additionally, the equilibrium static contact angle of the fully cross-linked hydrogel surface wetted with oil is measured using the sessile drop method. From a practical perspective, a method for optimizing and streamlining the high-tech manufacturing of cross-linked polymer microspheres and mini- and microchannel devices for use in bioengineering and pharmaceutical applications is suggested.

Design, characterization and implementation of cost-effective sodium alginate/water hyacinth microspheres for remediation of lead and cadmium from wastewater

The aquatic plant water hyacinth was dried then cross-linked with sodium alginate to produce ionic cross-linked microspheres. The mechanism of controlling cadmium (Cd) and lead (Pb) in wastewater was tested by DFT at B3LYP level using LANL2DZ basis set. Modeling results indicated that the hydrated metals could interact with sodium alginate (SA)/water hyacinth (WH) microspheres through hydrogen bonding. Adsorption energies showed comparable results while total dipole moment and HOMO/LUMO band gap energy showed slight selectivity towards the remediation of Pb. FTIR spectra of cross-linked microspheres indicated that WH is forming a composite with SA to change its structure into a microsphere to remove Cd and Pb from water. Raman mapping revealed that the active sites along the surface of the microspheres enable for possible adsorption of metals through its surface. This finding is supported by molecular electrostatic potential and optical confocal microscopy. Atomic absorption spectroscopy results confirmed that the microspheres are more selective for Pb than Cd. It could be concluded that WH cross-linked with SA showed the potential to remove heavy metals through its unique active surface as confirmed by both molecular modeling and experimental findings.

Computational design and preparation of water-compatible noncovalent imprinted microspheres

Herein, 2,4-dichlorophenoxyacetic acid (2,4-D) was used as a model template in a rational design strategy to produce water-compatible noncovalent imprinted microspheres. The proposed approach involved computational modelling for screening functional monomers and a simple method for preparing monodisperse and highly cross-linked microspheres. The fabricated non-imprinted polymer (NIP) and 2,4-d-imprinted polymer (2,4-d-MIP) were characterised, and their adsorption capabilities in an aqueous environment were evaluated. Results reveal that the pseudo-second-order kinetics model was appropriate for representing the adsorption of 2,4-D on NIP and 2,4-d-MIP, with R2 values of 0.97 and 0.99, respectively. The amount of 2,4-D adsorbed on 2,4-d-MIP (97.75 mg g−1) was considerably higher than those of phenoxyacetic acid (35.77 mg g−1), chlorogenic acid (9.72 mg g−1), spiramycin (1.56 mg g−1) and tylosin (1.67 mg g−1). Furthermore, it exhibited strong resistance to protein adsorption in an aqueous medium. These findings confirmed the feasibility of the proposed approach, providing a reference for the development of water-compatible noncovalent imprinted polymers.

Synthesis of poly(maleic anhydride-co-vinyl chloride) crosslinked microspheres and its superior enhancement in mechanical strength and antibacterial activity for poly(vinyl alcohol) composite films

Poly(vinyl alcohol) (PVA) is an attractive raw material and widely applicated in multiple fields owing to its unique features. However, the poor mechanical strength and without intrinsic antimicrobial activity severely restrained its further use. Herein, we proposed a novel strategy to fabricate PVA-based composites film with excellent mechanical properties and superior antibacterial activity by employing functional polymeric microspheres as a hydrogen bonding cross-linker and bactericide loading platform. Firstly, we synthesized poly(maleic anhydride-co-vinyl chloride) cross-linked microspheres (PDVM) via self-stabilized precipitation polymerization and subsequently the PDVM microspheres were reacted with gaseous ammonia to endow the microsphere surface with abundant carboxyl and amidogen group. As a result, uniform PDVM microspheres with diameters ranging from 175 to 685 nm and controlled gel content (25.3–91.8%) were prepared successfully and aminolyzed PDVM microsphere (NPDVM) were obtained. By incorporating 5 wt% NPDVM in PVA matrix, the composite film has a significant increment in tensile strength (63.9 MPa) and elongation at break (107 %) in comparison to neat PVA (51.8 MPa, 80%) mainly attributed to the formation of multiple hydrogen bonding network. Furthermore, by immobilizing Zn2+ on the surface of NPDVM/PVA film via complexation, the composite film exhibited outstanding antibacterial efficiency against E. coli and S. aureus.

Effects of Surface Morphology and Type of Cross-Linking of Chitosan-Pectin Microspheres on Their Degree of Swelling and Favipiravir Release Behavior.

The cross-linked microspheres were prepared and loaded with Favipiravir SARS-CoV-2 antiviral drug, by copolymerization of chitosan (CS) with a polysaccharide extracted from fresh pomegranate peels. Moreover, glutaraldehyde (Glu) has been used as a chemical cross-linker and sodium hexametaphosphate (SHMP) as a physical cross-linker. The extracted polysaccharide was analyzed, and different techniques have been used. The analyses lead to the conclusion that it is pectin. The surface morphology of the prepared microspheres was studied using a scanning electron microscope, where the size and shape factor (S) of the Glu microspheres showed high values (74.27 μm) and (0.852), respectively, meaning their surfaces tend to be rough, whereas the SHMP microspheres showed a smaller size particle (20.47 μm) and a smaller shape factor (0.748), which gives an indication that the SHMP microspheres have smooth surfaces. The swelling studies have shown that Glu microspheres have a higher degree of swelling, which means SHMP microspheres are more compact. The prepared microspheres have shown a higher loading percentage of Favipiravir antiviral drug in SHMP microspheres (37% w/w) in comparison with Glu microspheres (35% w/w), where the electrostatic interaction between the Favipiravir ions and SHMP anions helps for more loading. The microspheres prepared under different types of cross-linking have shown initial burst release of Favipiravir, followed by a step of controlled release for a certain period of time, whose period depends on the pH of the release medium. Both Glu and SHMP cross-linked microspheres have shown high controlled release times in buffered release solutions at pH = 7.4 and for shorter periods at pH = 1.3 and pH = 9.4, which may be related to the type of electrostatic interactions between drug and polymer systems and their reactions with release solution ions.

SPRAY-DRIED CHITOSAN MICROSPHERES FOR SUSTAINED DELIVERY OF TRIFLUOPERAZINE HYDROCHLORIDE: FORMULATION AND IN VITRO EVALUATION

Objective: Sustained release systems have the potential to enhance the therapeutic responses in the long-term management of psychiatric disorders. In the present study, cross-linked microspheres of the antipsychotic drug Trifluoperazine (TFP) were prepared using biodegradable polymer-chitosan and various in vitro evaluations were performed on the prepared microspheres. Methods: The spray drying technique was used to prepare TFP-loaded chitosan microspheres. Tripolyphosphate (TPP) was incorporated into the chitosan solutions as a cross-linking agent in varying concentrations. Different evaluations like production yield, encapsulation efficiency, drug-polymer compatibility, Scanning Electron Microscopy (SEM), X-ray diffraction studies (XRD), Differential Scanning Colorimetry (DSC), particle size, zeta potential analysis and in vitro drug release studies were performed on the developed formulations. Results: The formulated microparticles exhibited production yields ranging from 38.51 to 57.21% and had reasonably good encapsulation efficiencies (54.52-78.35%). The drug excipient compatibility was confirmed by Infrared Spectroscopy. All the microspheres showed positive zeta potential with a mean diameter ranging from 1.45-3.61µ. SEM images revealed the formation of spherical particles with indentations on the surface. XRD and DSC studies confirmed the presence of an amorphous form of the drug inside the microspheres. The in vitro release profile of TFP from cross-linked chitosan microspheres was influenced considerably by changing the concentration of polymer and crosslinking agent in the formulation. The drug release from (0.5%) chitosan microspheres reduced from 91% to 79%, when TPP concentration was increased from 10% w/w to 30% w/w. All the formulations clearly showed a burst release of the drug in the initial hours and a subsequent sustained release profile. Conclusion: The results of this study suggest that TPP crosslinked spray-dried chitosan microparticles could be a promising method for developing a long-acting drug delivery system intended to effectively treat schizophrenia.

Multifunctional inorganic/organic nanocomposite microspheres - reinventing eggs for bone repair applications

This study reports for first time the development of bone-regenerative nanocomposite microspheres re-engineered from chicken eggs. We deployed a novel hybrid crosslinking technique combining chemical and thermal crosslinking to produce highly cross-linked microspheres consisting of organic egg white matrix and bioactive inorganic egg shell nanofillers. The nanocomposite microspheres exhibit enhanced mechanical properties and prolonged degradation profile. Ca2+ released from the microspheres are found to accelerate the biomineralization process, facilitate the stem cell differentiation, and promote the osteogenesis process. In addition, antibiotic drug levofloxacin has been loaded onto the proposed microspheres and the dual function of the microspheres in bacterial infection control and bone regeneration has been successfully demonstrated in animal models. Results show that our nanocomposite microspheres can serve as a promising multifunctional platform for bone repair applications.

A Facile Immobilization Strategy for Soluble Phosphazene to Actualize Stable and Safe Lithium-Sulfur Batteries.

Lithium-sulfur batteries (LSBs) have attracted extensive attention owing to their high energy density and abundant sulfur resources. However, LSBs are still restricted by the unsatisfactory electrochemical performance resulting from the shuttle effect of lithium polysulfide (LiPSs), and the potential fire hazard caused by inflammable ether electrolytes and polyolefin separators. Herein, a facile immobilization strategy for hexachlorocyclotriphosphazene (HCCP) is creatively applied to address the above issues simultaneously. Insoluble HCCP cross-linked microspheres (H-CMP) are firstly obtained at ambient temperature using tannic acid (TA) as a cross-linking agent and then a multifunctional separator coating is constructed based on H-CMP. The released phosphorus-related radicals from H-CMP in wide temperatures effectively prevent the combustion of electrolytes and separators, and hence improve the fire safety of the Li-S pouch cell. Furthermore, H-CMP availably chemisorbs LiPSs to interdict the shuttle effect, thereby dramatically improving the electrochemical performance of LSBs. The effectiveness of this strategy is also verified in high sulfur loading (6.38mg cm-2 ), high temperature (50 °C), and Li-S pouch cells. More importantly, H-CMP exhibits sufficient stability for Li metal and suppression of Li dendrites. This facile immobilization strategy for multifunctional phosphazenes provides a competitive option for the large-scale fabrication of high-safety and high-performance LSBs.

Formulation, characterization and cellular toxicity assessment of a novel bee-venom microsphere in prostate cancer treatment

Bee venom (B.V.) is a toxin produced naturally by honey bees with several toxic and therapeutic efficacies. It is used in the treatment of different cancer kinds like renal, hepatic, and prostate cancer. Due to its protein nature, it is degraded in the upper gastrointestinal tract. Colon-targeted drug delivery systems represent a useful tool to protect B.V. from degradation and can be administered orally instead of I.V. infusion and traditional bee stinging. In the present study, B.V. loaded enteric-coated cross-linked microspheres were prepared by emulsion cross-linking method. Percentage yield, entrapment efficiency %, swelling degree, and in-vitro release are evaluated for prepared microspheres. Free B.V., optimized microspheres formula (F3), and doxorubicin cytotoxic effects were tested by MTT assay. Results concluded that free B.V. was more effective against the growth of human prostate adenocarcinoma (PC3) cells followed by optimized microspheres than doxorubicin. But both free B.V. and doxorubicin have a cytotoxic effect on normal oral epithelial cells (OEC). According to flow cytometric analysis, the optimized microsphere formula induced apoptosis and reduced necrosis percent at IC50 concentration. Furthermore, microspheres did not affect the viability of OEC. These results revealed that microspheres have a degree of specificity for malignant cells. Therefore, it seems that this targeted formulation could be a good candidate for future clinical trials for cancer therapy.

Interaction of alginate with nano-hydroxyapatite-collagen using strontium provides suitable osteogenic platform

BackgroundHydrogels based on organic/inorganic composites have been at the center of attention for the fabrication of engineered bone constructs. The establishment of a straightforward 3D microenvironment is critical to maintaining cell-to-cell interaction and cellular function, leading to appropriate regeneration. Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.ResultsPhysicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups. Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation. PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth. Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.ConclusionSr2+ cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts.Graphical

Preparation of Smart Surfaces Based on PNaSS@PEDOT Microspheres: Testing of E. coli Detection.

The main task of the research is to acquire fundamental knowledge about the effect of polymer structure on the physicochemical properties of films. A novel meta-material that can be used in manufacturing sensor layers was developed as a model. At the first stage, poly(sodium 4-styrenesulfonate) (PNaSS) cross-linked microspheres are synthesized (which are based on strong polyelectrolytes containing sulfo groups in each monomer unit), and at the second stage, PNaSS@PEDOT microspheres are formed. The poly(3,4-ethylenedioxythiophene) (PEDOT) shell was obtained by the acid-assisted self-polymerization of the monomer; this process is biologically safe and thus suitable for biomedical applications. The suitability of electrochemical impedance spectroscopy for E. coli detection was tested; it was revealed that the attached bacterial wall was destroyed upon application of constant oxidation potential (higher than 0.5 V), which makes the PNaSS@PEDOT microsphere particles promising materials for the development of antifouling coatings. Furthermore, under open-circuit conditions, the walls of E. coli bacteria were not destroyed, which opens up the possibility of employing such meta-materials as sensor films. Scanning electron microscopy, X-ray photoelectron spectroscopy, water contact angle, and wide-angle X-ray diffraction methods were applied in order to characterize the PNaSS@PEDOT films.

Study on the Adsorption of Heavy Metals in Sludge by Calcium Alginate Cross-Linked Zeolite Microspheres

Study on the Adsorption of Heavy Metals in Sludge by Calcium Alginate Cross-Linked Zeolite Microspheres

Superhydrophobic Surface for Enhancing the Bioavailability of Salbutamol Sulfate from Cross-Linked Microspheres: Formulation, Characterization, and in vivo Evaluation.

IntroductionThe aim of the work was to formulate salbutamol sulfate (SB) microspheres by using superhydrophobic surface (SHS) under different processing factors for improving its encapsulation efficiency, controling its release rate, and hence enhancing its bioavailability.MethodsCross-linked microspheres of chitosan (CN) and carrageenan (KN) were made on a SHS under a glutaraldehyde-saturated atmosphere. The formulations were designed and optimized based on 42 factorial design. Percentage encapsulation efficiency (%EE), particle size, swelling ratio, and in vitro release rate were characterized, and the in vivo performance of optimized formula was investigated in beagle dogs.ResultsThe results showed that the prepared microspheres have a high %EE (97.11±0.78%) for F13. The swelling ratio was 4.2 at the end of the 8 hours for the optimized formula, and the in vitro release rate was controlled for 12 hours. In vivo study verified that there was a 1.61-fold enhancement in SB bioavailability from optimized formula (F13) compared to market tablet.ConclusionThe study suggested that microspheres prepared from CN/KN crosslinking on an SHS using glutaraldehyde atmosphere is a promising technique that can encapsulate and sustain the release of water-soluble drugs such as SB in addition to improving its in vivo pharmacokinetic profile.

Tunable high internal phase emulsions stabilized by cross-linking/ electrostatic deposition of polysaccharides for delivery of hydrophobic bioactives

We have explored a controllable approach to produce polysaccharide-based high internal phase emulsions (HIPEs) without the use of any surfactants, synthetic particles, or synthesis of Pickering emulsions. This strategy required only the centrifugation of ultrasonication-produced shell cross-linked polysaccharide microspheres, and simultaneously provided facile control over the properties of HIPEs. By this means, the strong viscoelastic HIPEs with high oil volume fraction (up to 85.6%) can be stabilized solely by 0.5 wt% polysaccharide. We can further strengthen the HIPEs by generating an additional complex shell through the electrostatic deposition of non-cross-linked polysaccharides back on the cross-linked microsphere surface. Using β-carotene as a model hydrophobic bioactive, these HIPEs stabilized by cross-linking/electrostatic deposition of polysaccharides featured high encapsulation efficiency, oxidation stability, and pH/redox dual stimuli-responsive release. Further development of this strategy could exhibit a great potential to construct tunable HIPEs with desired functionalities for smart delivery and oral adsorption of hydrophobic food active ingredients.

Self-Peristaltic Gel-Microspheres Based on Carboxymethyl Cellulose and Polyacrylic Acid Prepared via Inverse Suspension for Recovery Rare Earth Ions from Aqueous Solution

In this study, cross-linked microspheres formed by interpenetrating networks of carboxymethyl cellulose and polyacrylic acid were prepared via inverse-suspension polymerization. The resultant has high adsorption capacity for rare earth ions, can reach 313 mg/g for Ce3+ under the condition of pH 6 at normal temperature. Unique self-creep characteristic gives it a fast-speed adsorption performance, which makes it to reach adsorption equilibrium within 70 min. It also has good reuse performance; its adsorption capacity decrease rate is only 21% after six adsorption–desorption cycles. Adsorption kinetics and thermodynamic studies and adsorption isothermal analysis were researched as well. The research conclusion suggested that this adsorbent is a promising adsorbent for recovering the rare earth ions from wastewater containing rare earth. And all related experiments show that ISP method is effective for making spherical macromolecular adsorbents via water-soluble monomers.

Microfluidic synthesis of monodisperse porous polystyrene microspheres for sorption of bovine serum albumin

Droplet microfluidics offers exquisite control over the flows of multiple fluids in micro-scale, enabling fabrication of advanced microspheres with precisely tuneable structures. The main goal of this work was to design monodispersed carboxylated polystyrene microspheres with a developed pore structure (a specific surface area more than 200 m2/g) using microfluidic technology. We investigated the influence of the composition of monomer phase for the stable formation of droplets. Under the stable region, the resulting microspheres (with diameter 50 µm) showed narrow size distribution having a coefficient of variation of below 2%. The obtained microspheres are characterised by morphology and surface structure by means of electron microscopy. The structure of cross-linked microspheres is investigated by solid-state 1H NMR spectroscopy. Finally, these microspheres have great potential for the effective sorption of biologically active substances (bovine serum albumin).

Degradable porous carboxymethyl chitin hemostatic microspheres

Lethal hemorrhage could endanger lives. Although many hemostatic agents are commercially available with good clinical effect, it is necessary to develop novel hemostatic materials with high efficacy, biological safety, low cost, easily preparation and excellent biodegradability and biocompatibility. Here, novel biodegradable and uniform microspheres with regular spherical architecture and porous microstructure were prepared by thermosensitive carboxymethyl chitin (CMCH) with low substitution degree and low deacetylation through physical-crosslinking at high temperature without using any toxic crosslinkers. The obtained CMCH microspheres (CMCH-MS) were non-cytotoxic, low hemolytic potential and biodegradable in the presence of lysozyme. In vitro blood clotting evaluation indicated that the porous microsphere network structures and the hydrophilicity of the CMCH could promote hemostasis due to the quick blood absorption and local concentration of the coagulation factors for the CMCH-MS. The CMCH-MS showed much better hemorrhage control than negative control and traditional hemostatic chitosan, similar hemostatic performance as commercialized cross-linked starch microspheres in both rat tail and liver models. Thus, the new biodegradable porous CMCH-MS may hold great potential for hemorrhage control in medical treatment.

AlCl<sub>3</sub> Cross-Linked and Spray Dried Carboxymethyl Sago Cellulose Microspheres as Potential Carriers for the Enteric Delivery

A semisynthetic polymer of carboxymethyl sago cellulose (CMSC) was synthesized from Malaysian sago biomass and further used in the development of drug delivery system. Recently, we have reported spray-dried carboxymethyl sago cellulose (CMSC) microspheres for enteric release and dissolution enhancement of piroxicam. In the present investigation, an attempt has been made to improve the enteric release property of CMSC microspheres using aluminium chloride as a cross-linker in the spray drying process and prednisolone as a model drug. CMSC microspheres loaded with prednisolone were prepared using a cross-linker concentration of 0%, 0.01%, 0.025% and 0.05%. All the drug-loaded microspheres were found to have high drug entrapment efficiencies (DEE) ranging from 99% to 106.1%. FT-IR spectroscopy has confirmed the cross-linking in CMSC microspheres as well as intact and amorphous nature of the entrapped drug. Field Emission Scanning Electron Microscope (FESEM) results have shown agglomeration of microspheres and the presence of drugs on the surface. Cross-linked microspheres have shown better efficiency than the uncross-linked microspheres in restricting drug release in stomach pH. Only about 5% of the loaded drug was released from cross-linked microspheres at pH 1.2 while 10% of the drug was released from uncross-linked microspheres. Also, cross-linked microspheres have exhibited faster drug release in pH 6.8 than the uncross-linked microspheres. Spray-dried and AlCl3 cross-linked CMSC microspheres have shown promising results in enteric drug delivery as well as dissolution enhancement.

Preparation of Black Poly(Methyl Methacrylate-Ethyleneglycol Dimethacrylate) Microspheres Using Metallic Complex Dyes

In this study, we synthesized polymethyl methacrylate cross-linked microspheres and functionalized the surface of the microspheres with amine groups, and discussed the effect of functionalization and the change of the surface morphology of the microspheres. This research uses 1:2 metal composite acid dye to dye self-synthesized microspheres with uniform particle size, and successfully prepares black polymethyl methacrylate cross-linked microspheres, which are applied to spacer microspheres.

Methacrylic acid functionalized CPS microspheres to adsorb shikimic acid

Chloromethylated cross-linked polystyrene (CMCPS) microsphere is hydroxylated, obtaining hydroxylated cross-linked polystyrene microsphere (CPS-OH). Methacrylic acid (MAA) is grafted on the surface of CPS-OH microspheres by cerium salt-hydroxyl group redox initiation system, obtaining graft-polymerization functional microsphere CPS-g-PMAA. The chemical structure and physicochemistry characters of CPS-g-PMAA are characterized with infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The adsorption performance of the grafted microspheres CPS-g-PMAA for shikimic acid was mainly investigated and the adsorption mechanism was explored. The experiment results show that for the graft-polymerization of MAA, the suitable concentration of MAA in solution is 3.2 mol/L, the optimal concentration of initiator cerium salt and catalyzer Sulfuric acid is 1.3 × 10−2 and 0.3 1 mol/L, respectively, the appropriate temperature is 55 °C, and reaction time is 6 h, the grafting degree of PMAA can reach 43.3 g/100 g. The kinetics demonstrating adsorption kinetic of SA by CPS-g-PMAA is fitted PFO model in the first 50 min and is then fitted with the PSO model. The CPS-g-PMAA microsphere adsorbs SA via hydrogen bond interaction, the adsorption capacity reaches 92 mg/g in ethanol. The adsorption amount decreases with the increase of temperature, Freundlich isothermal model is fitted the adsorption experimental data.