Introduction. Wide hybridization is an important source of new genes for resistance to diseases for efficient breeding of wheat (Triticum aestivum L.) in particular due to the involvement of a wide range of an alien diversity and the ability to combine wheat and the alien resistance genes.Aim. Investigate the resistance to widespread diseases and give the breeding evaluation of the advanced introgression lines developed by repeated crosses of amphiploids, primitive lines or collection samples with modern cultivars of winter bread wheat.Methods. The field experiments were established in the crop rotation of the department of wheat breeding and seed production of PBGI–NCSCI according to the generally accepted scheme of the breeding process of self-pollinating crops. The degree of plant damage was determined on a 9-point integrated scale developed on the basis of a modified Saari and Precott scale. The protein content was determined by the Kjeldahl method, the weight of thousand kernels (WTK) by the standard method (DSTU 4138-2002).Results. A low frequency of combine of the group resistance with high yield and grain quality was registered. The genetic background of some introgression lines has been found to be favorable for realizing the positive effect of rye translocation 1BL.1RS on both economically valuable and adaptive traits in Southern Ukraine and the ability to combine with other resistance genes. A failure of a correlation of the resistance to powdery mildew, leaf and stem rusts with crop yield both in the absence and presence of a natural infectious pressure, as well as low positive connection (Rsp = 0,26 **) of the productivity with resistance to Septoria blight and yellow rust in only one variant of the experiment were established. A low significant positive correlation between the resistance and protein content and WTK, as well as between indexes of the line resistance to various diseases was observed in most cases, which is obviously a result of the artificial selection for group resistance. The breeding lines (E2792_14, AIL1161_16, E218_09, E212_09, AIL1073_16) with alien polygenic complexes for the resistance to rust diseases, high values of WTK, protein content and morphological traits; characterized by high productivity, adaptability, baking quality were isolated.Conclusions. The lines are devoid of many of the negative qualities inherent to wild species, they can be a promising source for resistance to the diseases and may be of interest for further breeding work in Southern Ukraine. Кey words: Triticum aestivum L., introgression lines, resistance, productivity.