The marine shales in southern China are characterized by high thermal evolution and intensive deformation during multistage burial and uplift. Fracturing and cementation, associated with tectonic activity, diagenesis, hydrocarbon generation and migration processes, are widely and variably distributed in shale reservoirs experiencing different tectonic evolution. In this study, we utilized cross-cutting relationships, fluid inclusion microthermometry, and laser Raman spectroscopy of fluid inclusions, along with U-Pb dating of fracture-filling calcite veins, integrated with burial history modeling, to elucidate the timing and mechanism of fracturing in the Paleozoic Wufeng-Longmaxi shale reservoir, as well as regional variations in the Fuling shale gas field. Bedding-parallel fractures (BFs) in shale reservoirs are filled with calcite and quartz veins. The timing of fracturing, as determined by minimum homogenization temperatures and U-Pb dating, is estimated to have occurred during ca. 191 -122 Ma, during which some short vertical (or high-angle) fractures (VFs) opened around 163.4 Ma and 137 Ma, subsequently sealed by calcite veins, respectively. All calcite and quartz veins within these fractures contain high-density methane inclusions, while shale reservoirs were in a high-over-mature evolutionary stage. This suggests that fracturing was primarily driven by gas generation overpressure during deep burial. In shale reservoirs near fault belt folds, multistage fractures developed, including three stages of intersecting fractures (IFs) and one-stage of long VFs. The timing of fracturing and cementation corresponds to the Yanshanian tectonic uplift (ca. 83 - 69 Ma) with intense tectonic movement likely being the direct cause of fracture opening. The presence of abundant gas inclusions in veins recorded shale gas expulsion during rapid tectonic uplift, reflecting the destruction of shale gas preservation conditions. In contrast, no significant fracturing or cementation processes have been observed in the tectonically gentle zones. The different fracturing and cementation processes indicated that preservation conditions declined with increased fracture openings during uplift, correlating with the gas enrichment.