Abstract The International Health Regulations (IHR) are a legal instrument aimed to control infectious disease epidemics worldwide and that makes countries accountable for monitoring and promptly notifying the World Health Organization (WHO) of events that are likely to represent a public health emergency. The 196 signatory countries are bound by this international agreement. This workshop aims to present evidence on the role and updates of the IHR as a framework for global health preparedness. It will do that by exploring the main components of the IHR and their practical implications for public health practice, with particular emphasis on their function in preventing, detecting, and responding to cross-border public health threats. The workshop will feature four short presentations, in a total of 15 minutes, beginning with three presentations showcasing the findings of literature reviews covering various aspects of the IHR. These topics include the main challenges to implementation, the effectiveness of the IHR during the COVID-19 pandemic, its relevance to travel medicine and zoonosis prevention, and strategies for controlling vector-borne diseases. The last presentation will delve into the challenges and opportunities associated with implementing the IHR at both national and international levels, in the context of the proposals from the Working Group on Amendments to the International Health Regulations (2005). Throughout the workshop, emphasis will be placed on understanding how the IHR facilitate collaboration and coordination among countries during health emergencies, as well as the importance of capacity-building, information sharing, and multisectoral collaboration. This structure will allow for discussion aimed at areas for improvement to the IHR, especially in the context of its revision process and global health governance. We believe the added value of this workshop will be creating a platform for critical analysis and dialogue, sharing insights into the evolution of the IHR, ongoing debates, controversies, and emerging trends in global health regulations. Key messages • This workshop provides a platform for in-depth exploration of the IHR’s role in global health preparedness, offering evidence on its components and practical implications. • Through analysis of challenges and discussions on disease control, attendees will engage in critical dialogue aimed at improving the IHR and enhancing global health governance.