Crop Technology Research Articles

CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in pigeonpea and groundnut.

The CRISPR/Cas9 technology, renowned for its ability to induce precise genetic alterations in various crop species, has encountered challenges in its application to grain legume crops such as pigeonpea and groundnut. Despite attempts at gene editing in groundnut, the low rates of transformation and editing have impeded its widespread adoption in producing genetically modified plants. This study seeks to establish an effective CRISPR/Cas9 system in pigeonpea and groundnut through Agrobacterium-mediated transformation, with a focus on targeting the phytoene desaturase (PDS) gene. The PDS gene is pivotal in carotenoid biosynthesis, and its disruption leads to albino phenotypes and dwarfism. Two constructs (one each for pigeonpea and groundnut) were developed for the PDS gene, and transformation was carried out using different explants (leaf petiolar tissue for pigeonpea and cotyledonary nodes for groundnut). By adjusting the composition of the growth media and refining Agrobacterium infection techniques, transformation efficiencies of 15.2% in pigeonpea and 20% in groundnut were achieved. Mutation in PDS resulted in albino phenotype, with editing efficiencies ranging from 4 to 6%. Sequence analysis uncovered a nucleotide deletion (A) in pigeonpea and an A insertion in groundnut, leading to a premature stop codon and, thereby, an albino phenotype. This research offers a significant foundation for the swift assessment and enhancement of CRISPR/Cas9-based genome editing technologies in legume crops.

Economic Injury Levels and Economic Thresholds for Leucoptera coffeella as a Function of Insecticide Application Technology in Organic and Conventional Coffee (Coffea arabica), Farms.

Leucoptera coffeella (Lepidoptera: Lyonetiidae) is one of the main pests in coffee crops. The economic injury level (EIL) is the lowest density of the pest at which economic damages match the costs of control measures. The economic threshold (ET) is the density of the pest at which control measures must be taken so that this population does not reach the EIL. These are the main indices used for pest control decision-making. Control of L. coffeella is carried out by manual, tractor, airplane or drone applications. This work aimed to determine EILs and ETs for L. coffeella as a function of insecticide application technology in conventional and organic Coffea arabica crops. Data were collected over five years in commercial C. arabica crops on seven 100 ha central pivots. The cost of control in organic crops was 16.98% higher than conventional. The decreasing order of control cost was manual > drone > airplane > tractor application. Coffee plants were tolerant to low densities (up to 15% mined leaves) of the pest that caused losses of up to 6.56%. At high pest densities (54.20% mined leaves), losses were high (85.62%). In organic and conventional crops and with the use of different insecticide application technologies, EIL and ET were similar. The EIL and ET were 14% and 11% of mined leaves, respectively. Therefore, these indices can be incorporated in integrated pest management programs in C. arabica crops. The indices determined as a function of insecticide application technology in organic and conventional coffee are important as they serve producers with different technological levels. Additionally, EILs and ETs can contribute to more sustainable production, as control methods will only be employed when the pest density reaches these indices.

Determinants of adoption of climate smart agricultural technologies in wheat production in Arsi Zone, Oromia Region of Ethiopia

AbstractClimate-resilient crop production requires the use of crop technology that can sustainably increase productivity, build resilience to climate change, and reduce greenhouse gas emissions. Despite countrywide initiatives that promote climate-smart agricultural technology (CSAT) adoption, the rate of adoption has been extremely low. Therefore, this study aims to identify factors that affect the adoption of CSATs in wheat production in the Arsi zone of the Oromia region of Ethiopia. Towards this, plot-level primary data were gathered from 628 plots that were managed by 422 randomly selected wheat-producing smallholder farmers in three selected districts of the Zone. Descriptive statistics and multivariate probit (MVP) model were used to analyze data. The major CSATs adopted for wheat production in the study area were improved wheat varieties (95%), integrated soil fertility management (85%), and irrigation (19%). The result of the MVP model revealed that farmers with higher levels of education, farming experience, and contact with extension workers, have credit access, have access to market information, have greater annual total income, and are closer to the nearest market center are more likely to adopt CSATs in wheat production. Therefore, it is recommended that to enhance the adoption of CSATs in wheat production, policymakers and other development partners should concentrate on the identified factors that were significantly associated with the decisions to adopt CSATs.

The Influence of Sugar Beet Cultivation Technologies on the Intensity and Species Biodiversity of Weeds

Sugar beet production is highly affected by weeds. The structure of crop rotation, the use of intercrops and different tillage techniques bring several benefits to sugar beet cultivation and create different living conditions for weeds. The response of weed communities in sugar beet stands has not been studied. The experimental plot is in the cadastral area of Ivanovice na Hané (Czech Republic). During an eight-year monitoring period (2013–2020), 46 weed species were identified. The dominant species was Chenopodium album. There were also summer and winter weeds. A more varied crop rotation increased the intensity of weed infestation, with winter weeds being the most common. On the contrary, a higher proportion of cereals in the crop structure favors the presence of summer weeds. The tillage technology and the inclusion of catch crops did not significantly affect the intensity of weed infestation in sugar beet stands or the spectrum of weed species. Current cropping technologies have driven the evolution of weeds. Due to their short life cycles and relatively simple genomes, weeds can respond very quickly to technological measures and, thus, change their harmfulness.

Optimization of an Efficient Direct Shoot Induction System and Histological Evaluation of Shoot Formation in Cucurbita maxima Duch

Improving plant regeneration ability and shortening regeneration time can promote the development of genetic transformation breeding technology for horticultural crops. We optimized several culture conditions, including explant type, mother plant genotype, and medium, to improve shoot formation in winter squash (Cucurbita maxima Duch.). Histological analysis of the occurrence of shoots was also carried out. The results indicate that cotyledon was the most suitable explant for inducing the shoot regeneration of winter squash. We found that ‘Jin-li’ had a shorter shoot induction time and a higher average number of shoots. The highest induction rate of 95.23% among the five lines. The average shoot induction rate of five lines was the highest (84.85%) on Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 6-benzylaminopurine (6-BA) and 0.2 mg/L indole-3-acetic acid (IAA). We also found that there was an interaction between genotypes and induction media, and their interaction had a greater impact on the shoot induction rate than individual effects. Histological observation revealed that the induced shoots of winter squash cotyledons originated from subepidermal cells. We also found that the optimal medium for de novo root regeneration was 1/2 MS. We acclimatized and cultivated regenerated plants and harvested their fruits, which maintained the characteristics of mother plants. These findings lay an important foundation for further research on direct shoot regeneration and accelerate its application in winter squash genetic transformation.

Optimizing nitrogen application position to change root distribution in soil and regulate maize growth and yield formation in a wide-narrow row cropping system: pot and field experiments.

The wide-and narrow-row cropping technology used for maize has the advantages of protecting cultivated soil and improving the population structure in maize fields. However, the relationship between nitrogen application position and root interactions has not been determined. Through pot and field experiments, we evaluated the effects of two nitrogen application positions ((narrow row nitrogen application (RC) and wide row nitrogen application (RN)) and two nitrogen application regimens ((high nitrogen(HN) and low nitrogen(LN)) on root growth and yield composition of wide-narrow row maize during the flowering and harvest stages. In field experiments, RC increased the biomass, length and surface area of competing roots (narrow-row roots, CR) at the flowering stage. The yield and agronomic efficiency of N(AEN) and partial factor productivity of N(PFPN) were increased by RN compared to RC under HN, However, the AEN under LN was significantly lower; There was no significant effect on maize growth and biomass allocation at the same level of application of N. At the flowering stage, the results of CR and non-competing roots (wide-row roots, NCR) was consistent under pot experiments and the field experiments, and the yield under RN was also higher than that under RC, although the difference was not significant. Furthermore, according to the principal component analysis and correlation analysis, the competing roots were the main factor influencing yield and AEN. In conclusion, our study showed that RN is a useful fertilization method to improve overall productivity. All in all, how roots coordinate neighbors and nitrogen spatial heterogeneity is a complex ecological process, and its trophic behavior deserves further study.

Advancing tree genomics to future proof next generation orchard production.

The challenges facing tree orchard production in the coming years will be largely driven by changes in the climate affecting the sustainability of farming practices in specific geographical regions. Identifying key traits that enable tree crops to modify their growth to varying environmental conditions and taking advantage of new crop improvement opportunities and technologies will ensure the tree crop industry remains viable and profitable into the future. In this review article we 1) outline climate and sustainability challenges relevant to horticultural tree crop industries, 2) describe key tree crop traits targeted for improvement in agroecosystem productivity and resilience to environmental change, and 3) discuss existing and emerging genomic technologies that provide opportunities for industries to future proof the next generation of orchards.

Winter Cover Crops Affect Aggregate-Associated Carbon, Nitrogen and Enzyme Activities from Black Soil Cropland

The thinning, leaning, and hardening of arable land in the black soil region of Northeast China has brought serious challenges to the sustainable development of agriculture. It is of great significance to explore suitable conservation tillage for the conservation and sustainable utilization of black soil resources actively. The topsoil of the cropland in the northeastern part of the Songnen Plain with winter fallow (CK), planted alfalfa, and planted winter wheat was used as the research object to analyze the changes in the soil aggregate composition, nutrients, and enzyme activities before and after freeze–thaw, respectively, and to investigate the effect of winter cover crops on the improvement of the quality of the black soil cropland. Compared with the winter fallow field, (1) planting alfalfa significantly increased the mechanical stability of 1–2 mm and 0.25–1 mm particle size aggregates (about 3 times and 25 times over), and planting winter wheat increased the water stability of 0.25–1 mm particle size aggregates significantly (2.7 times over); (2) planting alfalfa and winter wheat increased the soil C/N ratio of >2 mm and 1–2 mm particle size aggregates, and the increment in the C/N ratio in >2 mm particle size aggregates remarkably increased, by 203.6% and 362.7%, respectively; (3) planting alfalfa significantly enhanced the soil invertase activity and urease activity in >2 mm and 0.25–1 mm particle size aggregates, and planting winter wheat significantly enhanced the catalase activity in 0.25–1 mm particle size aggregates. In conclusion, planting winter cover crops during the winter fallow period can maintain and promote the mechanical and water stability of medium and large (0.25–1 mm,1–2 mm) soil aggregates, increase the carbon content and C/N ratio of larger (1–2 mm, >2 mm) aggregates, and enhance the enzyme activity of small and medium (0.25–1 mm, <0.25 mm) aggregates to varying degrees. The results of the study can provide a reference for the promotion of basic research on and technology for winter cover crops in the black soil region.

The economic impact of CGIAR-related crop technologies on agricultural productivity in developing countries, 1961–2020

The international agricultural research centers that comprise the CGIAR got their start in the 1960s. They soon made major contributions to crop improvement in developing countries, particular in rice and wheat in Asia. Today, farmers can acquire new technologies from many sources, and evidence of whether the CGIAR continues to play an important role in crop improvement has become dated. This paper brings together an expanded set of evidence on the diffusion and productivity impact of CGIAR crop research through 2020, and breaks out these impacts by crop, region, and over time. By 2016–2020, CGIAR-related crop technologies had been adopted on at least 221 million hectares across Asia, Africa and Latin America, generating economic welfare gains of $47 billion annually. In the 2010s, technology adoption and welfare impacts were increasing by about $600 million annually, almost as much as in the 1990s. In the early days of the “Green Revolution,” these welfare impacts were largely confined to rice and wheat in Asia, but in recent decades have grown to include a larger range of crops and geographies, notably cassava and maize in Sub-Saharan Africa. Although improved crop varieties have been the main technology through which CGIAR crop centers have achieved these impacts, CGIAR-related integrated pest management and natural resource management technologies have also made significant contributions to crop productivity. In addition to raising farm income, productivity gains in staple crops have lowered food prices, thereby benefitting the whole population. This is a key reason why agricultural productivity growth, and food crop productivity growth in particular, has had greater impacts on poverty reduction in low-income countries than comparable productivity growth in other sectors.

Preface

Within the framework of the presented proceedings, the results of the conducted III International Conference on Current Issues of Breeding, Technology and Processing of Agricultural Crops, and Environment (CIBTA-III-2024).

An overview on production constraints, strategies and innovative production technologies of fruit crops

An overview on production constraints, strategies and innovative production technologies of fruit crops

ТЕХНОЛОГИИ УБОРКИ ЗЕРНА ЭНЕРГО-РЕСУРСОСБЕРЕГАЮЩИМИ МНОГОФУНКЦИОНАЛЬНЫМИ АГРЕГАТАМИ

The research was carried out with the aim of improving the technology of harvesting cereal crops for the production of competitive products. The work was carried out in 2018-2022 in Krasnodar region. We optimized the parameters of multifunctional units and tested them in relation to harvesting conditions in Kuban. Comparison of machines, complexes, technologies and systems was carried out using a comprehensive assessment method using the Harrington function. The proposed technology with multifunctional units, compared to the basic one, allows reducing labor costs by 1.25 times, operating costs and metal consumption by 1.2 times, and increasing labor productivity by almost 2 times. When analyzing the dependence of the cost of grain yield losses on the duration of harvesting, direct biological yield losses and indirect losses associated with injury to grain by the working parts of harvesting machines were taken into account. A multifunctional unit is proposed, designed for harvesting grain crops with simultaneous pressing of straw. It is based on the use of the serial power unit “Polesie” UES-2-280A, the mounted grain harvester KZR-10 and the straw baler PRP-1.6. A production test has proven the advantage of a rotary threshing apparatus compared to a classic one. The use of combine harvesters with rotary threshing and separating devices reduces grain crushing, compared to threshing machines, by 10 times, microdamage to grain - by 6...8%. Using the three-factor experiment planning method, the optimal duration for harvesting grain crops using the proposed technology with a multifunctional unit was established, which, with a grain yield of 6.8 t/ha, is 5 working days, the working width of the combine header is 5.4 m. Using the proposed unit with one of the trailed machines (straw baler, direct seed seeder, discor or other tillage machine) is quite effective.

Effects of seed size and nursery media on the germination and seedling growth of Plukenetia conophora

Research on the effects of seed size, soil-based and soilless media to determine the best condition for increased seedling production of African walnut was carried out at the green house of Department of Crop Science and Technology, Federal University of Technology, Owerri. Seed size was categorized into 10.23 to 10.31g (for large seeds) and below 10.23g (for small seeds). Soil-based nursery medium was sawdust mixed with topsoil volume by volume (v/v) 1:1, while soilless was sawdust alone. The experiment was a 2×3 factorial in a complete randomized design with 6 replications. Data were collected on number of days to emergence, plant height at 4, 8, and 12 weeks after planting (WAP), and number of leaves at 4, 8, and 12 WAP. Data collected were tested with analysis of variance (ANOVA), and treatment means were separated with Fishers least significant difference at 5% level of probability. Result showed that large seeds planted on soil-based media performed best in all the assessed traits. The inference is that high quality seedlings of African walnut can be produced by planting large seeds in soil-based media.

Screening and Selection for Herbicide Tolerance among Diverse Tomato Germplasms

Solanum lycopersicum, the domesticated species of tomato, is produced and consumed globally. It is one of the most economically important vegetable crops worldwide. In the commercial production of tomatoes, tomatoes are extremely sensitive to herbicide drifts from row crops in the vicinity. Injury to tomatoes from auxin herbicides and glyphosate can occur at rates as low as 0.01×. This results in a substantial yield reduction, and at high drift rates, plants may not show signs of recovery. With the new herbicide-resistant crop technologies on the market, which include 2,4-D and dicamba-resistant crops, there is an increase in the usage of these herbicides, causing more serious drift problems. There is a diverse germplasm of tomatoes that includes wild relatives which are tolerant to numerous biotic and abiotic stresses. Herbicide/chemical stress is an abiotic stress, and wild tomato accessions may have a natural tolerance to herbicides and other abiotic stresses. In the current study, diverse tomato genotypes consisting of 110 accessions representing numerous species, Solanum habrochaites, S. cheesmaniae, S. pimpinellifolium, S. chilense, S. lycopersicum, S. pimpinellifolium, S. galapagense, S. chimelewskii, S. corneliomulleri, S. neorickii, and S. lycopersicoides, were used for screening drift rate herbicide tolerance. The herbicides tested included simulated drift rates of 2,4-D, dicamba, glyphosate, quinclorac, aminopyralid, aminocyclopyrachlor, and picloram. The visual injury rating of each accession for each herbicide treatment was taken 7, 14, 21, and 28 days after treatment (DAT) on a scale of 0–100%. Numerous accessions were found to have minimal injury (less than 20%) for each of the herbicides tested; nine accessions were found for both 2,4-D and glyphosate, eleven for dicamba, five for quinclorac, eight for aminocyclopyrachlor and two for both aminopyralid and picloram at 28 DAT. The identification of genotypes with a higher herbicide tolerance will provide valuable genetic resources for the development of elite tomato varieties that can resist herbicide injury and produce competitive yields.

Effects of Cluster Farming and Crop Technology Dissemination on the Livelihoods of Farmers: The Case Chobi District West Shewa Zone of Oromia, Ethiopia

Enhancing productivity among smallholder farmers is widely perceived as a key strategy that is achieved through promoting an agricultural cluster farming approach. The adoption of improved agricultural production technologies is central to increasing production and productivity. This study was conducted in the Chobi district of the West Shewa zone with the objectives of this study to introduce improved crop technologies and to enhance crop productivity. In the Chobi district, six types of crop technologies were promoted in the 2019/20 production season. The district is a previously unaddressed area in improved crop production technology and a total of 234 farmers (197 male and 37 female) benefited with a total of 68.97 quintals disseminated improved seed. In the information-sharing field days held a total of 499 stakeholders participated. From the 13 improved varieties that cover 164.3 hectares of land, a total output of 3753 quintals of grain was harvested. The yield effect of different crop varieties in the Chobi district scores higher yield in barley 32 Qt/ha and maize 80 Qt/ha than the national and regional average. The yield from linseed is 11 Qt/ha higher than the national average and the productivity of potato 120 Qt/ha is higher than the regional mean yield. Therefore, the study recommends a cluster farming with a full crop production package is crucial to enhance the income gains of smallholder farmers and the concerned stakeholders have to engage in the dissemination of climate-smart and resilient crop production technologies and extension services.

The Role of Agricultural Projects in Building Sustainable and Resilient Maize Value Chain in Burkina Faso

Poor seed quality and climate change significantly affect the maize value chain in Burkina Faso. To address the challenges, a catalytic project titled “Strengthening resilient seed systems in the maize value chain in Burkina Faso—from research to markets” was initiated to enhance the development of a resilient maize value chain. This study aims to assess the role of the project in developing a sustainable and resilient maize value chain. In this study, we used a mixed approach in design and implementation: qualitative research using key informants’ interviews, secondary data such as baseline survey reports, and lessons learned during the seed value chain greening intervention implemented in the Hauts-Bassins and Cascades regions of Burkina Faso. We analyzed qualitative data following the Gioia method. Kabako, a drought-tolerant hybrid seed variety, doubled crop yields in demonstration plots compared to smallholder farms and regional and national averages. Extension officers and village-based advisors (VBAs) were trained on improved seeds, composting, strip cropping, intercropping, crop rotation, and water management technologies and afterward trained smallholders. The VBAs trained smallholders on proper postharvest management practices and processing. The off-takers acted as the market. However, smallholders also sold their maize products in the informal open markets. The aggregator system was the missing link in Burkina Faso’s maize value chain. There was limited involvement of women in the project. Results obtained from this study are valuable for policymakers and value chain actors in preparing policies and filling missing gaps for exploiting the potential of the maize value chain.

Large Scale Demonstration and Yield Performance of Selected Crop Technologies: Evidence from West Shewa Zone, Oromia Region, Ethiopia

Large Scale Demonstration and Yield Performance of Selected Crop Technologies: Evidence from West Shewa Zone, Oromia Region, Ethiopia

Management and application of specific location food crop technologies based on ecoregional dry land in central Sulawesi (A Review)

To achieve food self-sufficiency and food independence, government has been implementing some programs. Those programs generally face two basic problems, namely: 1. A high conversion of paddy fields in to industries and housing area which are in line with the growth of population. 2. Global climate change which causes more frequently of drought and floods in both dry and lowland farming. Dry land is very extensive in Central Sulawesi which can be used as a new source of food production to reach food self sufficiency, while it has not been optimally utilized yet. Thus, Central Sulawesi Province has potency to become a food buffer region in Indonesia by optimizing the utilization of dry land along with the application of specific location technologies. Some technologies which could be simultaneously applied in dry land are high-yield varieties selection, improving cropping patterns, rotation of varieties, modification of cropping systems, and specific location fertilization. This article will discuss or review the results of research that have been carried out in dryland management, the use of adaptive superior varieties, and planting patterns that can significantly increase land productivity and farmer income. So comprehensive planning is needed to improve and optimize the use of dry land. To speed up technology in certain locations, a collaboration between local governments, especially extension services, research institutions and universities is needed to encourage the production and productivity of food crops in dry lands.

Farmer Interest Groups: Paving Its Path Towards Profitability

The current study entailed to ascertain the role that Farmers' Interest Groups played in knowledge transfer as well as in increasing agricultural production, productivity, and in turn leading to profitability for the farm sector in the Boudh district of Odisha. For the impact assessment, six FIGs from a range of villages in the Boudh district, including Baghiapada, Saleising, and Palijhar, were randomly selected. The results of this study depicted that member of Farmer Interest Groups adopted scientific agricultural practices and goatery technologies at a greater mean rate (80.63 MPS and 79.63 MPS, respectively) than non-members (59.12 MPS and 60.12 MPS). The aggregate differences in the adoption of crop and goatery farming technology between FIG members and non-members were calculated to be 21.5 and 19.5 MPS, respectively. It was also found that the crop and goatery production under FIG produced a higher yield than the agricultural practices now employed by farmers. The members became more active in meetings and the adoption of new and scientific farm and goatery technology after learning about the benefits of the FIGs. In addition to enhancing agriculture and animal production, this will also benefit the socioeconomic conditions of the farming community.

Water-smart farming: review of strategies, technologies, and practices for sustainable agricultural water management in a changing climate in West Africa

The global temperature increase of 1.1°C over the past decade and human population growth are already escalating competition for water in locations where water is scarce, adversely affecting agriculture. Water-smart farming was developed to address challenges associated with the availability, access and use of agricultural water. It complements practices and goals related to agricultural water in climate-smart agriculture. It encompasses several climate-responsive food cropping systems, water-saving methods, tools, and technologies to grow food sustainably. Effective implementation of water-smart farming can ameliorate the pressing burden of agricultural water competition. Therefore, the conceptual framework of water-smart farming and its principal elements or ideology are introduced in this review. The major pillars of water-smart cropping, which include conditioning and improving the soil’s capacity to capture and hold moisture, basic infrastructure for water harvesting, storage and use as nature-based cropping solutions were elaborated. Further examples of technologies and best practices in water-smart crop farming, such as breeding for drought-tolerant crop varieties, promoting dryland cropping systems, alternate wetting and drying technology, water drainage and control structures, rainwater harvesting, utilization of irrigation reservoirs, cover cropping and intercropping for enhancing soil moisture conservation were presented. The review also briefly shows the contribution of some of these best practices and adaptive technologies of water-smart cropping toward promoting water-saving methods used for growing rice, maize, legumes, cassava and peri-urban vegetables.