ABSTRACT Food production systems in protected conditions are probably the most intensive agricultural systems causing biodiversity loss. Experimental sites in different European areas were established to investigate whether an agroecological approach to organic greenhouse production allows preserving or increasing biodiversity. Soil nematode communities were characterized by DNA-metabarcoding, and some biodiversity indices were calculated. A general increase in absolute number of genera indicates that the innovative treatments potentially positively influence biodiversity. A multidimensional analysis revealed that the change in nematode community compositions is caused more by country and sampling year than by the farming system. However, regionally, some shifts in biodiversity are obvious and the impact by a farming practice likely. This study is the first indication that the implementation of some agroecological measures in European protected cropping systems is feasible as at least they did not worsen (nor improve) soil biodiversity.
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