Defoliant spraying is an important link in the mechanized cotton harvest because adequate and uniform spraying can improve defoliation quality and reduce cotton trash content. In defoliant application, application volume and spraying technology are extremely important. In this study, the effectiveness of defoliant application to cotton plant that has come to harvest with two different application volumes and three different types of nozzles with a standard field crop sprayer was determined. Application rates were 250 and 400 L/ha and spraying nozzles were: (1) standard flat fan nozzle (TP8006), (2) air induction nozzle (AI 11002-VS) and (3) dual pattern nozzle (AI307003VP). A tracer (BSF) and defoliant were applied to mature cotton with approximately 60% open bolls and samplings for BSF deposition and spray coverage on cotton plant were done at two plant height (upper layer, lower layer) of plant. Before and after spraying, bolls open and leaves rate on cotton plants were calculated and filter papers were used to detect BSF deposition and water sensitive papers (WSP) were used to measure coverage rate of spraying methods used. Spectrofluorophotometer was used to detect the amount of tracer deposition on targets and an image process computer program was used to measure coverage rate on WSP. In analysis conclusions showed that air induction nozzle (AI 11002-VS), achieved better results than the dual pattern and standard flat fan nozzles in terms of higher depositions, coverages and leaf defoliations and boll opening rates. AI nozzles operating at 250 L/ha application rate provide the highest deposition and coverage rate on applications of defoliant, in addition, BSF as an indicator of the defoliant used reached on leaf beneath in merely this spray nozzle. After defoliation boll opining rate was 85-% on the 7th and 12th days after spraying and falling rate of leaves was 76-% at application rate of 250 L/ha with air induction (AI1102) nozzle.