AbstractA soil test for estimating mineralizable N in soil is needed to improve fertilizer use efficiency. Our objectives were to: (i) test the effectiveness of two chemical indices in estimating potentially mineralizable N (N0) in soils, and (ii) determine if these indices could be used to differentiate the impact of cultural practices on the N‐supplying capacity of soils. We collected samples from the 0‐ to 0.15‐m depth of 42 soils representing all agroecological regions in Saskatchewan, Canada. We determined (i) N mineralized (Nmin), N0, and the rate constant (k) by aerobic incubation at 35°C for 24 wk, and (ii) the NH4‐N extracted by (a) 2 M KCl heated at 100°C for 4 h, and (b) a steam‐distilled phosphate‐borate solution buffered at pH 11.2. The association between N0 and the hot 2 M KCl extracted NH4‐N was close (r2 = 0.78, significant at P < 0.001); the relationship between N0 and the phosphate‐borate NH4‐N was slightly less precise (r2 = 0.73, P < 0.001). When NH4‐N extracted with cold 2 M KCl was subtracted from the NH4‐N extracted with hot extractants, the association with Nmin was weaker (r2 ≤ 0.50). The value of k for the 42 soils was generally constant (avg. 0.067 ± Sxt 0.05 = 0.040 wk−1). In a second experiment, we compared the two chemical extractants vs. the N mineralized in a 24‐wk incubation regarding their effectiveness in differentiating the effects of eight 37‐yr crop rotation treatments on N‐supplying capacity. These three indices performed effectively in this regard. For example, the 2 M KCl NH4‐N was closely associated with N mineralized in 24 wk (r2 = 0.92), as was phosphate‐borate NH4‐N (r2 = 0.88; both significant at P < 0.001). We concluded that the chemical extractants may provide a quick test for assessing N‐supplying capacity of the soil.