Cromileptes Research Articles

Survival Rate and Growth Response of Humpback Grouper Seeds (Cromileptes altivelis) on Acclimatization to the Freshwater

Successful biodiversity cultivated in freshwater fish is farmed whitefish, tiger shrimp, vannamei shrimp and. While the grouper duck (Cromileptes Mozambique) export market prospects have never tried. This research would like to know how the seed of acclimatization grouper duck into fresh water against the survival rate and growth response. Using random design complete 5 x 4 treatment, seed samples with long range 12 cm, weight 22 gr. Acclimatization of habitat in salinity of 30 ppt ppt 4. Moderate decrease in salinity 0.0 A0 is the ppt/day (controls), A0,5 (0.5 ppt/day), A1 (1 PPT/day), A1.5 (1.5 ppt/day), and A2 (2 ppt/day). Data were analyzed with the survival rate of the t test (P ≤ 0.05) the results show a decrease in salinity of 0.5 ppt/day 35% real different with controls 92.5%, but is superior than the three other treatments (A1, A1.5 and A2) dead all before reaching 4 ppt. While the growth rate data were analyzed with anova followed duncan test (P ≤ 0.05) the results show A0.5 0.60% no different with real control of 0.61% real but different (fastest) compared to three other treatments (A1, A1.5 and A2). The conclusion is grouper duck capable cultivated in brackish water 4 ppt (mesohalin) through acclimatization with decreased salinity of 0.5 ppt/day.

Studi Identifikasi Dan Prevalensi Endoparasit Pada Saluran Pencernaan Ikan Kerapu Tikus (Cromileptes altivelis) Di Keramba Jaring Apung Unit Pengelola Budidaya Laut Situbondo, Jawa Timur <br><i>[Prevalence Study And Identification Of Endoparasites On Humpback Grouper Gastrointestinal (Cromileptes altivelis) In Floating Net Cage Marine Culture Management Unit Situbondo, East Java]<i>

Abstract Humpback grouper (Cromileptes altivelis) or also known as polkadot grouper is one of the excellent fish commodities in Indonesia which has trading value reached $ 90-150/kg. As a consumed fish, humpback grouper are needed for beverage at luxuries restaurant and hotel in the world. As major export commodities in Indonesia, since the 1990's the techniques of grouper cultivation in float net rats are enhanced to fulfill international market demand. Humpback grouper fish cultivation techniques in float net cages cannot be separated from the problems in any cultivate activities, one of the causes is a disease and one of the diseases that rushed humpback grouper is parasite. The disadvantages that caused by the parasite is fish’ weight loss. The purpose of this study is to determine the types of endoparasites that attacked the digestive system of humpback grouper (Cromileptes altivelis) in float net cages and also to discuss the prevalences of endoparasites that attack the digestive of humpback grouper (Cromileptes altivelis) in float net cages. The method of this research is descriptive. The sampling was done in once for 60 fishes with the length range of fish is around 15-20 cm and 4 months old from the float net. The number of each fishes samples that were taken 5-10% from the total of population, where the number of humpback grouper populations in each raft float net are 200 fishes. The main parameters that observed in this study were endoparasites identification species that attack the digestive system of humpback grouper (Cromileptes altivelis) in float net cages and the prevalence rates for each endoparasites. The supporting parameters in this study were the water quality in float net cages which includes temperature, pH and salinity are measured during the sample taken. The results showed that 60 samples taken from four float nets 6 fishes were positive infected endoparasitic of trematodes and nematodes, besides 4 fishes are positive infected worms Echinostoma which belong to the class of Trematodes and 2 fishes infected worms Camallanus carangis that belongs to the class Nematoda. The prevalence values for each of the worm are 6.67% for Echinostoma and 3.33% for Camallanus carangis. The research concern the identification and prevalence of endoparasites in Humpback grouper at hatchery is needed to early finding out the attack of endoparasites and to break the flow of endoparasitic spreading.

Kultur Sel Otak Ikan Kerapu Bebek (Cromileptes altivelis) Dengan Menggunakan Media L-15, MEM Dan TCM <br><i>[Cell Culture Brain Of Chromileptes altivelis With Medium L-15, MEM And TCM]<i>.

Abstract Humpback grouper is high value commodity fish. The demand of domestic and foreign markets increases every year. The obstacles of Humpback grouper fish cultivation is a disease caused by Viral Nervous Necrosis (VNN). The virus attacks the brain leading to mass death. To prevent the disease, a research on VNN virus has to be started. The problem of the research is the lack of culture of fish tissue. Virus have specific location to tissue cell and species to be infected. Thereofore, first step must be done is growth from tissue cell which infection of virus. Cell culture is a technique to isolate cell, protoplasm, tissue or organ to be grown in a sterile condition with nutrition containing growth hormone to promote multiplication. Cell culture technique can be used in many basic applications in cell biology. The purpose of this research is to develop cell culture from Grouper fish brain for VNN research. The hope is culture can grow and develop well.Research executed at June until Desember 2009 in Laboratory In Vitro of Veterinari Faculty of Airlangga University and Laboratory Gastroenteritis Institute of Tropical Disease, Airlangga University, Surabaya. The result showed that brain cell culture of humpback grouper fish can be grow and multiply well in all three media, media L-15 cell growth was faster compared to media MEM and TCM.

Assessing Vulnerability of Fish in the U.S. Marine Aquarium Trade

The trade in coral reef fishes for aquariums encompasses over 1,800 species from over 40 exporting countries, yet the population status for most traded species is unknown and unevaluated. At the same time, these coral reef fishes face a growing number of threats and often occur in jurisdictions with limited management capacity and data. In response, we assess vulnerability to overfishing for 72 coral reef fishes popular in the aquarium trade for the United States – the top importer – from the top exporting countries (Indonesia and the Philippines). We use a data-limited assessment approach: productivity susceptibility analysis (PSA). PSA estimates relative vulnerability of species by assessing their biological productivity and susceptibility to overfishing. The most and least vulnerable stocks were differentiated by attributes related to the reproductive biology (e.g., breeding strategy, recruitment pattern, and fecundity), appropriateness, ease of capture (e.g., schooling and aggregation), and rates of natural mortality. Our analysis identifies several of the most and least vulnerable species popular in the aquarium fish trade. The species that ranked as least vulnerable to overcollection were Gobiodon okinawae, Nemateleotris magnifica, Gobiodon acicularis, Salarias fasciatus, Ptereleotris zebra, Gobiodon citrinus, Pseudocheilinus hexataenia, Chaetodon lunula, Nemateleotris decora, and Halichoeres chrysus. In contrast, the ten most vulnerable species were Chromileptes altivelis, Plectorhinchus chaetodonoides, Pterapogon kauderni, Premnas biaculeatus, Echidna nebulosa, Centropyge bicolor, Zebrasoma veliferum, Pomacanthus semicirculatus, Zebrasoma scopas, and Thalassoma lunare. In a data-limited context, we suggest how these vulnerability rankings can help guide future efforts for reducing vulnerability risk. In particular, species that are relatively high-vulnerability and high-volume are prime targets for research and aquaculture efforts, increased monitoring of collection and exports, species-specific stock assessments, and voluntary reductions by retailers and consumers to avoid overexploitation.

Uji Toksisitas Surfaktan Linear Alkilbenzene Sulfonate (LAS) Histologi Insang dan Hati Benih Ikan Kerapu Bebek (Cromileptes altivelis)

Kristianti et al, 2019. Toxicity of Linear Alkilbenzene Sulfonate (LAS) Surfactant Gills and Liver Histology of Humpback Grouper Seed (Cromileptes Altivelis). JLSO 8(1):107-116.LAS is surfactant that is most commonly used because this surfactant is the main cleaning substance contained in detergent. Waste of detergent can give side effects in the tissue of organisms, especially on the gills and liver of fish. The aims of this research were to analyze the toxic effects arised from LAS surfactant to the survival of fish seed experiment with different concentrations, to determine levels of LAS surfactant that are capable to be tolerated and to analyze the gills and liver damage arising from LAS surfactant to fish seed experiment. The test of LAS surfactant toxicity on fish seed experiment was conducted using the laboratory of experimental method, while the testing of gills and liver histology of fish seed experiment with paraffin method and Hematoxylin Eosin (HE) staining. The results showed that LAS surfactant gave toxic effects on Humpback Grouper Seed (Cromileptes altivelis) and gave effect such damage to the gills and liver histology. Toxicity and tissue damage rose as the increase of LAS surfactant concentration. LAS surfactant levels were able to be tolerated by Humpback Grouper Seed test not more than 0,253 mg/l.

Analysis of Transportation Development for Small Islands in Sumenep District, East Java Province

This study aims to describe the economic potential of islands in Sumenep District. This study uses a qualitative descriptive approach, carried out by collecting data through observation, in-depth interviews, and data tracking. Sumenep District has the largest number of islands in East Java, 287 islands, spread across nine subdistricts. These islands have considerable economic potential, from oil and gas to fisheries, epinephelinae, cromileptes altivelis, and lobster. This area has a great need for the existence of a transportation network as a means of economic mobility of the community, which also affects the price of basic necessities far higher than the area of Sumenep Regency on the mainland, Madura Island and Java Island.

Genotype determination of megalocytivirus from Indonesian Marine Fishes

Murwantoko, Sari DWK, Handayani CR, Whittington RJ. 2018. Genotype determination of megalocytivirus from Indonesian Marine Fishes. Biodiversitas 19: 1730-1736. Megalocytivirus is the newest genus within the family of Iridoviridae which can be divided into groups represented by red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV) and turbot reddish body iridovirus (TRBIV), threespine stickleback iridovirus (TSIV). This virus caused serious systemic disease in cultured marine fishes for consumption and ornamental freshwater fishes with significant mortality. The objective of this study was to determine the genotype of megalocytivirus which infected marine fishes from Lampung, Karimun Jawa, Situbondo and Batam based on major capsid protein (MCP), ATPase, DNA polymerase, CY15 and IRB6. The liver, spleen and kidney tissues of humpback grouper (Cromileptes altivelis), tiger grouper (Epinephelus fuscoguttatus), baramundi (Lates calcarifer) were fixed in 10% phosphate-buffered formalin for histological and fixed in 70% ethanol for molecular analysis. Molecular analysis was performed by amplification of MCP, ATPase, DNA polymerase, CY15 and IRB6 genes and followed by sequencing. Genotype was determined by alignment of the sequences with various genotypes of megalocytivirus from Genbank. Histological examination showed that hypertrophy, inclusion body forming bearing cells were found in liver, spleen and kidney tissues. Polymerase chain reaction with MCP primer produced specific DNA bands. Those results confirmed the infection of megalocytivirus on marine cultured fish samples. The analysis from 10 isolates on five genes revealed that two genotypes of megalocytivirus as infectious spleen and kidney necrosis virus (ISKNV) and red sea bream iridovirus (RSIV) genotypes were existed in Indonesia. The ISKNV genotype was confirmed in fish samples from Lampung, Jepara, Bali; while RSIV genotype was found in fishes from Batam, and Situbondo. Interestingly, both ISKNV and RSIV genotypes were confirmed in fish samples from Karimun Jawa. This paper is the first report on the present of ISKNV and RSIV genotypes in Indonesia based on MCP, ATPase, DNA polymerase, CY15 and IRB6 genes.

Effects of dietary administration of Lactococcus lactis HNL12 on growth, innate immune response, and disease resistance of humpback grouper (Cromileptes altivelis)

Lactic acid bacteria are a common group of probiotics that have been widely studied and used in aquaculture. In the present study, we isolated Lactococcus lactis HNL12 from the gut of wild humpback grouper (Cromileptes altivelis) and explored its probiotic properties. For this purpose, L. lactis HNL12 was added to the commercial fish feed. The results showed that HNL12 had high auto-aggregation ability and strong tolerance to simulated gastrointestinal stress. When C. altivelis consumed a diet containing 0 (control), 106, 108, or 1010 CFU/g HNL12 for four weeks, all of the groupers fed a diet with HNL12 had significantly increased percent weight gain (PWG), especially those fed with 108 CFU/g, which had a PWG of 231.45%. Compared to the control, fish fed with L. lactis HNL12 exhibited significantly increased survival rates following injection with Vibrio harveyi after one month. Immunological analysis showed that C. altivelis fed with HNL12 had (i) enhanced respiratory burst activity of head kidney macrophages, superoxide dismutase, acid phosphatase, and lysozyme activities of serum; (ii) an improved survival rate from 36% to 70%; and (iii) upregulated expression of a broad spectrum of immunity. Meanwhile, de novo transcriptome assembly yielded 89,314 unigenes, which were annotated by at least one of the reference databases (Nr, Swiss-Prot, GO, COG and KEGG). A total of 307 genes showed significantly different expression between the groups fed with or without added HNL12. GO and KEGG enrichment analyses of the significantly different expression gene categories and pathways were related to infectious diseases, antigen processing and presentation, and other immune system responses. These results indicate that L. lactis HNL12 is effective for enhancing the growth, immunity, and disease resistance of C. altivelis; this study also provides insight into the use of probiotics for commercial applications.

Karakterisasi Molekuler Bakteri Probiotik Ikan Kerapu Bebek Berbasis Teknik 16S Ribosomal DNA

The aim of research was to find the molecular characteristics of probiotic bacteria in the digestive tract of grouper fish (Chromileptes altivelis) based on 16S ribosomal DNA technique. The bacteria were isolated from stomach and intestine of the fish and were analized in the Marine Microbiology Laboratory of fishery and marine Science Faculty of Riau University. The bacterial DNA were isolated by using a PCR (polymerase Chain Reaction) and was conducted in the biotechnology laboratory of Diponegoro University, Semarang. The purified DNA was sequenced in the BPPTTangerang. Result shown that 6 bacterial species may be potential as probiotic. There were Bacillus velesensis strain CR-11, Vibio alginolyticus A3G-2, Bacillus cereus site2S, uncultured bacterium clone BB3S16S-17, Bacillus subtillus strain CICC10066, and Bacillus flexus strain LF-3. these bacteria grow well at pH 2 and this indicated one of probiotic bacteria characteristics.

ANALISIS KESESUAIAN PERAIRAN UNTUK BUDIDAYA IKAN KERAPU BEBEK (Cromileptes altivelis) DI PERAIRAN PULAU TEGAL TELUK LAMPUNG

Tegal Island is part of Lampung Bay which has potential to be the location of aquaculture. One of kind fish that can be cultivated in these area is the humpback grouper (Cromileptes altivelis). Humpback grouper is the most popular fish species in local and foreign markets that have the highest selling price. The purpose of this study were to know the physical and chemical parameters in Tegal Island waters, to know the suitability of the waters for the humpback grouper cultivation in the waters of Tegal Island. The research was conducted in May 2017. The sampling location was 4 stations. The range of water quality values in Tegal island were water depth (15-25 m), dissolved oxygen (7 – 8 mg/l), brightness (5-10 m), salinity (30-34 ppt), temperature (28 – 32°C ), current velocity (20 – 50 m/s), pH (7,7 – 8,0), nitrate (0,9 – 3,2 mg/l) and phosphate (0,2 – 0,5 mg/l). The results showed that the waters of Tegal Island had appropriate water suitability level (St 3), corresponding marginally (St 1 and St 2) and unsuited (St 4) for the cultivation of humpback grouper.

The Benefit of Decision Support System as Sustainable Environment Technology to Utilize Coastal Abundant Resources in Indonesia

Being the largest archipelagic state in the world (± 81 000 km of coastline), Indonesia has a great potential for developing the aquaculture industry. But, the accomplishment of sustainable practices and management systems are still in its infancy. Therefore, it is vital to improve aquaculture technology and develop management tools that address the need for an eco-friendly production process. In this manuscript, the Decision Support System software is applied for the management of sustainable floating net cage finfish cultures. This software integrates physical, chemical, biological properties and information on coastal uses to determine site selection, production carrying capacity, ecological carrying capacity and socio-economic assessment of 18 cases, focusing on the economic viability of Tiger grouper [Epinephelus fuscoguttatus (Forsskål, 1775)], Humpback grouper [Cromoliptes altivelis (Valenciennes, 1828)] and Leopard Coral grouper [Plectropomeus leopardus (Lacepède, 1802)] at three remote areas in Indonesia, including: Ekas Bay, Talise and Galang Island. The outcomes demonstrate that only Galang Island provides a suitable area of 12 940 ha with the estimated production carrying capacity of (0.5 to 366) t/annual per fish farm along with ecological carrying capacity are limited of (18 393 to 21 727) t/annual and economic evaluation highlighted that all cultures development are economically viable.

Identification of Plectropomus leopardus, Promicrops lanceolatus and Cromileptes altivelis using species-specific TaqMan real-time PCR

Plectropomus leopardus, Promicrops lanceolatus and Cromileptes altivelis are high-value groupers in chinese aquatic product market and are substituted by cheaper ones sometimes due to economic profit. In this study, real-time PCR methods for identification of these groupers were developed. Species-specific primers and probes were designed on the basis of cytochrome oxidase I (COI) mitochondrial DNA sequences of groupers and other fishes. The specificity of the real time PCR assays were tested with a total of 51 specimens of fish, meat and poultry. Amplifications were observed in specific species only. The methods developed were accurate and rapid and offered the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.

The application of pigment-protein fraction from Nannochloropsis oculata on β-actin response of Cromileptes altivelis infected with viral nervous necrosis

<p class="Pa3"><strong>ABSTRACT </strong></p><p> </p><p class="Pa5">β-actin is a prominent protein in the immune system. An outcome from gene transcription, the protein protects against pathogen infection, such as receptor clustering, antigen internalization, and regulating vesicle for antigen processing. ß - actin expression will determine the success of immune response of the organism. This study aimed to understand the role of pigment-protein fraction (PPF) from <em>Nannochloropsis oculata </em>in the increase of β-actin expression in humpback grouper <em>Cromileptes altivelis </em>infected by <em>viral nervous necrosis </em>(VNN). The experiment was performed with one negative control (A: normal fish) and three PPF treatments (B: fish + PPF, C: fish + VNN, and D: fish + PPF + VNN). PPF was applied through the sonde method and analyzed with immunohistochemistry technique and Immunoratio software. The results showed that PPF was able to increase β-actin expression on all treatments: A (34.9%), B (38.1%), C (39.1%), and D (51.6%). It demonstrated PPF ability to induce the increase of β-actin expression indicating an improved defense of humpback grouper <em>C. altivelis </em>against VNN infection.</p><p> </p><p class="Pa5">Keywords: β-actin, pigment-protein fraction, <em>Nannochloropsis oculata</em>, VNN</p><p> </p><p> </p><p class="Pa3"><strong>ABSTRAK</strong></p><p class="Pa3"><strong> </strong></p><p class="Pa5">β-aktin adalah protein yang berfungsi penting sebagai salah satu sistem pertahanan tubuh. Protein ini merupakan hasil transkripsi dari gen yang memiliki fungsi penting dalam sistem pertahanan tubuh terhadap infeksi patogen, seperti kelompok reseptor, internalisasi antigen, dan mengatur keluar masuknya vesikel untuk pemrosesan antigen. Ekpresi β-aktin menjadi penentu berhasil atau tidaknya respons imun pada tubuh organisme. Penelitian ini bertujuan untuk mengetahui peran fraksi protein pigment (FPP) dari <em>Nannochloropsis oculata </em>dalam meningkatkan respons β-aktin pada ikan kerapu tikus <em>Cromileptes altivelis </em>yang terinfeksi viral nervous necrosis (VNN). Pengamatan dilakukan pada empat percobaan yaitu, satu kontrol (A : ikan normal) dan tiga perlakuan FPP (B : ikan + FPP, C: ikan + VNN, dan D: ikan + VNN + FPP). Pemberian FPP menggunakan metode sonde dan analisis hasil penelitian menggunakan teknik imunohistokimia dan <em>Immunoratio</em>. Hasil penelitian ini menunjukkan bahwa pengiduksian FPP mampu meningkatkan ekpresi β-aktin yaitu A (34,9%), B (38,1%), C (39,1%), dan D (51,6%). Hal tersebut menunjukan FPP mampu menjadi <em>inducer </em>yang baik untuk peningkatan ekpresi β-aktin. Meningkatnya ekspresi β-aktin bisa dijadikan sebagai indikator pertahanan tubuh ikan kerapu tikus <em>C. altivelis </em>terhadap infeksi VNN.</p><p> </p><p>Kata kunci: β-aktin, fraksi protein pigmen, <em>Nannochloropsis </em>oculata, VNN</p>

Development of Humpback Grouper Fish (Chromileptes altivelis) Culture in Gebe Island, Central Halmahera, North Moluccas

Gebe Island as one of the islands in Central Halmahera of North Moluccas Province, has a high potential of fishery resources both for capture and aquaculture fisheries. This activity aimed to improve community skill to rear humpback grouper fish (Chromileptes altivelis ) in floating net at Umera and Umiyal Villages as community empowerment in the Gebe Island. The method of this activities were training and mentoring. Juvenile of the fish was from artificial breeding, and collected naturally from the sea around the Gebe Island. According to the observation of the activity, the community can rear the humpback grouper fish from 5 cm to 2025 cm total body length per individual for 12 months (Umera Village), and it was from 5 cm to 1015 cm total body length per individual for 10 months (Umiyal Village). The fish grew to be 300 g/ind (in December 2013), 500 g/ind (July 2014), and 600 g/ind (October 2014). The community has been able to harvest and sell the grouper fish collected from the sea, reared for 24 months in the floating net.

GENETIC VARIATION AND POPULATION STRUCTURE IN THE HUMPBACK GROUPER, Cromileptes altivelis, TROUGHOUT ITS RANGE IN INDONESIAN WATERS

Starch gel electrophoresis was used to assess the level and distribution ofgenetic variation in humpback grouper, Cromileptes altiuelis sampled from 4 different areas of coral reefs in Raas and Kangean located in Madura as well as Sangeang and Bungin located in Sumbawa regions, between December 1997 and March 1998.

EFFECT OF CHOLINE AND LECITHIN ON GROWTH AND FEED EFFICIENCY OF JUVENILE HUMPBACK GROUPER, Cromileptes altivelis

This experiment was conducted to determine the effect of supplemental choline and lecithin in the diet of juvenile humpback grouper.

EFFECT OF DIETARY PROTEIN AND ENERGY ON GROWTH OF JUVENILE HUMPBACK GROUPER (CromiIeptes altivelis)

The effect of varying protein and energy contents of diet on the groMh and nutrient retention of juvenile humpback grouper was examined using a 3 x 3 factorial design with three replicates.

SUBSTITUTION OF FISHMEAL WITH SOYBEAN MEAL IN HUMPBACK GROUPER, Cromileptes altivelis JUVENILE DIETS SUPPLEMENTED WITH PHYTASE

Feeding experiments were conducted to evaluate the effects of replacing fishmeal with soybean meal in di6t on grolvth of humpback grouper.

GROW OUT AND EVALUATION OF FIRST GENERATION (F1) OF PROSPECTIVE HUMPBACK GROUPER Cromileptes altivelis BROODSTOCK

During this time the humpback grouper broodstock spawning that comes from nature. Efforts to provide humpback grouper fish from cultured (F1) has been conducted and it has been spawn. The purpose of this study was to determine the quality of broodstock first generation (F1) with a good maintenance management and monitoring evaluation of character growth, gonad development, and genetic characteristics through microsatelite marker. Broodstock reared in concrete tank volume of 75 m3 with three different of hormonal implantation treatments: (1) estradiol, (2) 17α-methyltestosterone (MT), (3) a mixture of 17α-Methyl-testosterone by aromatase inhibitors, and control. The observed parameters were growth, gonad development, reproductive hormones in the blood composition and the performance of genetic through microsatellite analysis. The results showed that the humpback grouper F1 implantation treatment by methyl testosterone and estradiol showed good growth with an average body weight at the end of the observed ranged from 680 ± 60.5 to 820 ± 76,5g with an average body length of 34 -36 cm. In individual control growth better because during the maintenance of the population is not implanted. Analyses of testosterone and estradiol in the blood plasma showed that 40% of individuals in the population were males. Microsatellite analysis showed that F1 fish had good genetic variation (0.778-1.000) so that it can be used as broodstock candidate.

GROW OUT AND EVALUATION OF FIRST GENERATION (F1) OF PROSPECTIVE HUMPBACK GROUPER Cromileptes altivelis BROODSTOCK

<em>During this time the humpback grouper broodstock spawning that comes from nature. Efforts to provide humpback grouper fish from cultured (F1) has been conducted and it has been spawn. The purpose of this study was to determine the quality of broodstock first generation (F1) with a good maintenance management and monitoring evaluation of character growth, gonad development, and genetic characteristics through microsatelite marker. Broodstock reared in concrete tank volume of 75 m<sup>3</sup> with three different of hormonal implantation treatments: (1) estradiol, (2) 17α-methyltestosterone (MT), (3) a mixture of 17α-Methyl-testosterone by aromatase inhibitors, and control. The observed parameters were growth, gonad development, reproductive hormones in the blood composition and the performance of genetic through microsatellite analysis. The results showed that the humpback grouper F1 implantation treatment by methyl testosterone and estradiol showed good growth with an average body weight at the end of the observed ranged from 680 ± 60.5 to 820 ± 76,5g with an average body length of 34 -36 cm. In individual control growth better because during the maintenance of the population is not implanted. Analyses of testosterone and estradiol in the blood plasma showed that 40% of individuals in the population were males. Microsatellite analysis showed that F1 fish had good genetic variation (0.778-1.000) so that it can be used as broodstock candidate. </em>