Saffron Extract Research Articles

Study the Possible Protective Effect of Saffron Extract on Induced Rats Toxicity by Acrylamide

Study the Possible Protective Effect of Saffron Extract on Induced Rats Toxicity by Acrylamide

Eco-friendly silver nanoparticles (AgNPs) fabricated by green synthesis using Capsicum annum L. extract: biosynthesis, characterization, and antibacterial activity

Silver nanoparticles (AgNPs) have many benefits in the health sector, it has antimicrobial and biomedical Activity. AgNPs can be synthesized by using green chemistry or using chemical substances, such as NaBH4. Green synthesis of AgNPs is an environmentally friendly alternative and has cost-effective production. Capsicum annuum L. combined with Saffron extract was used to synthesize AgNPs. The biosynthesis, characterization, and antibacterial activity of AgNPs were studied in this research. The synthesized AgNPs were characterized by UV- Visible spectroscopy (UV-Vis), Fourier- transform Infrared Spectroscopy (FTIR), and Particle Size Analyzer (PSA). The formation of AgNPs was confirmed by optical performance using UV-VIS spectroscopy and showing a peak of AgNPs at 407 nm. Capsicum Annuum L. combined with Saffron extract contained C-H, C-N, and C=O groups. The particle size was characterized using PSA, the size is 37,74 nm. Capsicum Annuum L. combined with Saffron extract has good antibacterial activity. Keywords: Green Synthesis, AgNPs, Capsicum annuum L, Saffron.

Microencapsulated saffron floral waste extracts as functional ingredients for antioxidant fortification of yogurt: Stability during the storage

Dairy industries are constantly seeking to develop innovative ingredients through the use of food by-products, since consumers’ demand for healthier foodstuffs has increased over the last years. Therefore, the development of innovative dairy products enriched with saffron floral extracts could represent a novel environmentally-friendly technological solution. The present study aimed to determine the effect of adding alginate-based microencapsulated saffron floral by-products extracts (0.5 g/100 g, 1 g/100 g), or/and saffron stigmas extracts (0.05 g/100 g, 0.1 g/100 g) to homemade yogurts. For this purpose, physical-chemical properties, antioxidant capacity, total phenolic content (TPC), microbiological analysis, color and organic acids and soluble sugars profile were determined at 0, 7, 14 and 21 days of refrigerated storage. The results showed that these novel yogurt formulations allowed proper fermentation, being the microbiological profile and physicochemical parameters not affected by saffron extracts, compared to the control. The incorporation of microencapsulated saffron floral extracts improved the functional properties in terms of TPC and antioxidant properties which remained stable over 21 days of storage. Enriched yogurts also showed a good profile of organic acids and soluble sugars, mainly lactic acid and lactose. In conclusion, saffron floral by-products-alginate beads could be potential sustainable candidates to use as functional ingredients improving the nutritional and functional value of yogurts.

Enhancement of cisplatin-induced apoptosis by saffron in human lung cancer cells

BackgroundCisplatin is a prevalent chemotherapeutic agent, and it has been used extensively to treat lung cancer. However, its clinical efficacy is hampered by its safety profile and dose-limiting toxicity. Saffron is a natural product that has shown significant anticancer effects. The combination treatment of saffron with chemotherapeutic agents has been considered a new strategy. MethodsHerein, saffron extract as a natural anticancer substance was combined with cisplatin to assess their combined efficacy against tumor development in vitro. In A549 and QU-DB cell lines, the combined effect of the saffron extract with cisplatin led to a significant reduction in cell viability as compared to cisplatin alone. ResultsAfter 48 h incubation a considerable reduction in ROS levels in the QU-DB cell line upon treatment with cisplatin in the presence of saffron extract in comparison with cells treated with cisplatin alone. Furthermore, apoptosis increased significantly when in cells treated with cisplatin in combination with saffron extract compared to cisplatin alone. ConclusionOur data establish that the combination of saffron extract as a natural anticancer substance with cisplatin leads to improved cell toxicity of cisplatin as an anticancer agent. Therefore, the saffron extract could be potentially used as an additive to enable a reduction in cisplatin dosages and its side effects.

Prevention of neurotoxicity and cognitive impairment induced by zinc nanoparticles by oral administration of saffron extract.

The accumulation of relatively higher dose of zinc oxide nanoparticles in brain was reported to produce neurotoxicity. Indeed, nanoparticles have a high ability to penetrate biological membranes and be uptaken by cells, which may cause cell disorders and physiological dysfunctions. The aim of the current study was to evaluate, whether oral administration of saffron extract, in rats, can protect from neurotoxicity and behavioural disturbances induced by chronic administration of ZnO-NPs. Daily oral administration of ZnO-NPs was performed for 21 consecutive days to induce oxidative stress-like situation. Then after the saffron extract was concomitantly administrated in several rat groups to overcome the nanotoxicological effect induced by ZnO-NPs. In the frontal cortex, the hippocampus and the cerebellum, ZnO-NPs induced a H2 O2 -oxydative stress-like effect reflected in reduced enzymatic activities of catalase, superoxide dismutase and glutathione S-transferase, and decreased acetylcholinesterase activity. In addition, increased levels of proinflammatory interleukins IL-6 and IL-1-⍺ occurred in the hippocampus, reveal the existence of brain inflammation. The concomitant administration of saffron extract to animals exposed to ZnO-NPs prevented the enhanced anxiety-related to the behaviour in the elevated plus-maze test, the open field test and preserved spatial learning abilities in the Morris water maze. Moreover, animals exposed to ZnO-NPs and saffron showed abnormal activity of several antioxidant enzymes as well as acetylcholinesterase activity, an effect that may underly the preserved anxiety-like behaviour and spatial learning abilities observed in these animals. Saffron extract has a potential beneficial therapeutic effect: antioxidant, anti-inflammatory and neuroprotective agent.

Eco-friendly synthesis of zinc oxide nanoparticles using saffron extract and their photocatalytic and antibacterial activities

Eco-friendly synthesis of zinc oxide nanoparticles using saffron extract and their photocatalytic and antibacterial activities

Optimasi Formula dan Evaluasi Sediaan Sabun Transparan dari Putik Saffron (Crocus Sativus L.)

Transparent solid soap, namely soap in the form of a bar with a transparent appearance, the resulting foam on the skin is softer. Saffron has been used as a food ingredient, treatment, and traditional medicine since ancient times. The content of saffron is like saponins which can be used in making transparent soap. This study aims to formulate a transparent soap from saffron essence as a skin protecting property. This research includes the manufacture of saffron extract, manufacture of transparent soap and transparent soap test parameters including organoleptic test, pH test, foam stability test, water content test and preference test by respondents. Based on research results, saffron extract can be formulated into transparent solid soap preparations that meet the requirements for water content in accordance with SNI, namely for F0 9.12 ± 0.56, FI 8.6 ± 0.42, F2 8.6 ± 0.43, F3 8.6±0.93. For the results of the foam height test, it was found that the foam results were quite stable. For the results of the irritation test, it can be concluded that soap preparations containing saffron essence are safe to use because they do not show any side effects after use.

Extracts obtained from organs of saffron (Crocus sativus) alter growth and seed germination of common lambsquarters (Chenopodium album) and barnyard grass (Echinochloa crus-galli)

With the aim of examining the allelopathic effects of saffron (Crocus sativus), Chenopodium album and Echinochloa crus-galli were selected for this study. The experiment was carried out in different levels using aqueous extract of different organs (leaf, corm and a combination of leaf and corm) with different concentrations of the extracts (0, 1, 2.5, 5, 10 and 20%). The results showed that seed germination of both weeds was significantly affected by the organs and concentration of saffron extracts. The highest germination rate of C. album was 2.38 seeds per day in the application of corms extract and the lowest germination rate was 2.12 seeds per day in the application of combination of leaf and corm extract. The highest seed vigor index of C. album was 12 in the application of leaf extract and the lowest was 9 in the application of leaf and corm extracts. In addition, the C. album application of the highest concentration of the extract (20%) led to the lowest weight of root, stem, seedlings, germination percentage, germination rate, seed vigor index, and number of normal seeds. In E. crus-galli, the increase in the concentration of the extract from 0 to 20% enhanced the mean germination time from 3.4 in control sample to 4.1 days with 20% extract concentration. All the applied extracts obtained from different saffron organs caused a significant reduction in germination traits in both species of weeds. Meanwhile the extracts of leaf and corm exerted the highest effects. Generally, the results showed that the application of 20% extract obtained from the combination of leaf and corm tissue provided the highest growth inhibition and lowest germination rate in both weeds.

Fingerprint of Characteristic Saffron Compounds as Novel Standardization of Commercial Crocus sativus Extracts.

Food supplements based on saffron (Crocus sativus L.) dried stigma extracts are widely consumed due to their multiple bioactive properties. Saffron extract (SE) standardization is of crucial importance, as it determines the reproducibility of the product quality and is essential for the evaluation of its bioactive effect and safety. Although SEs are commonly standardized considering their safranal content, the lack of specificity of the official methods may give inaccurate measurements. In addition to the development of more precise methodologies, the evaluation of alternative saffron components, such as crocins and picrocrocin, for standardization purposes would also be of interest. Thus, in this study, qualitative and quantitative information regarding picrocrocin and crocin isomers of different commercial saffron extracts was first obtained by a validated methodology using liquid chromatography (HPLC) coupled to diode array (DAD) and mass spectrometer (MS) detectors. Principal component analysis (PCA) was applied to gain insight into the compositional variability and natural grouping of SE. These studies suggested the potential use of the relative content of crocin isomers and trans-/cis-crocins and trans-4 GG/picrocrocin ratios as novel criteria for SE standardization. Their reproducibility and stability under controlled storage conditions for 36 months was demonstrated in a commercial standardized SE (affron®).

Identification of Compounds of Crocus sativus by GC-MS and HPLC/UV-ESI-MS and Evaluation of Their Antioxidant, Antimicrobial, Anticoagulant, and Antidiabetic Properties.

In order to valorize the species Crocus sativus from Morocco and to prepare new products with high added value that can be used in the food and pharmaceutical industry, our interest was focused on the phytochemical characterization and the biological and pharmacological properties of the stigmas of this plant. For this purpose, the essential oil of this species, extracted by hydrodistillation and then analyzed by GC-MS, revealed a predominance of phorone (12.90%); (R)-(-)-2,2-dimethyl-1,3-dioxolane-4-methanol (11.65%); isopropyl palmitate (9.68%); dihydro-β-ionone (8.62%); safranal (6.39%); trans-β-ionone (4.81%); 4-keto-isophorone (4.72%); and 1-eicosanol (4.55%) as the major compounds. The extraction of phenolic compounds was performed by decoction and Soxhlet extraction. The results of the determination of flavonoids, total polyphenols, condensed tannins, and hydrolyzable tannins determined by spectrophotometric methods on aqueous and organic extracts have proved the richness of Crocus sativus in phenolic compounds. Chromatographic analysis by HPLC/UV-ESI-MS of Crocus sativus extracts revealed the presence of crocin, picrocrocin, crocetin, and safranal molecules specific to this species. The study of antioxidant activity by three methods (DPPH, FRAP, and total antioxidant capacity) has proved that C. sativus is a potential source of natural antioxidants. Antimicrobial activity of the aqueous extract (E0) was investigated by microdilution on a microplate. The results have revealed the efficacy of the aqueous extract against Acinetobacter baumannii and Shigella sp. with MIC ≤ 600 µg/mL and against Aspergillus niger, Candida kyfer, and Candida parapsilosis with MIC = 2500 µg/mL. Measurements of pro-thrombin time (PT) and activated partial thromboplastin time (aPTT) in citrated plasma obtained from routine healthy blood donors were used to determine the anticoagulant activity of aqueous extract (E0). The anticoagulant activity of the extract (E0) studied showed that this extract can significantly prolong the partial thromboplastin time (p < 0.001) with a 359 µg/mL concentration. The antihyperglycemic effect of aqueous extract was studied in albino Wistar rats. The aqueous extract (E0) showed strong in vitro inhibitory activity of α-amylase and α-glucosidase compared with acarbose. Thus, it very significantly inhibited postprandial hyperglycemia in albino Wistar rats. According to the demonstrated results, we can affirm the richness of Crocus sativus stigmas in bioactive molecules and its use in traditional medicine.

Abstract 1319: Saffron (Crocus sativus) extract inhibits migration and invasion of cancer cells through modulation of MMP-3 and MMP-9 expression

Abstract Saffron is a spice that has been used throughout history for various therapeutic properties. Recently, it has also been documented to have a significant anticancer effect on primary tumors. However, limited studies are found studying its effect on the metastatic potential of cancers. The goal of our study was to explore the effect of saffron on the migration and metastatic potential of breast cancer cells. Methods: MDA468 breast carcinoma cells were treated with varying concentrations of Saffron (100-1000 ug/mL) and examined using Q-phase holographic time-lapse microscopy to measure the movement of cells over time. The anticancer activity of Saffron was confirmed using cell viability assays. Invasion and metastatic potential were studied using Boyden-chamber and scratch wound assays. Finally, the tumorigenicity of the cell line was examined through colony formation assays in the presence and absence of Saffron. Results: Q-phase microscopy revealed a reduction in projections and significantly reduced movements in cells after Saffron treatment. The inhibition of migration, cell mobility and invasion by Saffron was confirmed through scratch wound and Boyden chamber assays (p&amp;lt;.01). Saffron extract also resulted in inhibition of viability in a dose-dependent manner and colony formation assays found markedly reduced size and number of colonies in the presence of the extract. Western blot results showed marked reduction of MMP-3 and MMP-9 expression in Saffron-treated cells. Conclusions: Our results found saffron to be effective in decreasing breast cancer carcinoma cells MDA468’s viability, migration, and tumorigenicity. Initial results indicate that Saffron may reduce migratory ability of cancer cells through the modulation of metalloproteinases. Citation Format: Tyler King, Stefana Moscardini, McKenzie Martin, Fatima K. Rehman. Saffron (Crocus sativus) extract inhibits migration and invasion of cancer cells through modulation of MMP-3 and MMP-9 expression [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 1319.

Crocus sativus Extract as a Biological Agent for Disease-Modifying Therapy of Collagenase-Induced Mouse Model of Osteoarthritis.

Osteoarthritis (OA) is an age-related joint disease that involves the degeneration of cartilage and is the most prevalent form of arthritis, affecting a large part of the population. OA is a multifactorial disorder, and no single etiological mechanism has been found to be common to all forms of the disease. Currently used therapies for control of the disease are mainly nonsteroidal anti-inflammatory drugs (NSAIDs) and corticosteroid medications. The aim of this study was to investigate the extract from Crocus sativus as a biological disease-suppressing therapy agent. Balb/c mice were injected intra-articularly with Clostridium histolyticum type IA for induction of osteoarthritis. The mice were randomized to five groups: control group, I group (CIOA untreated), II group (CIOA + 100 mg/kg/daily saffron), III group (CIOA + 50 mg/kg/daily saffron), IV group (CIOA + 25 mg/kg/daily saffron). Flow-cytometry analysis was used to study the splenocytes' phenotype isolated from the treated animals. The serum levels of inflammatory and anti-inflammatory cytokines were analyzed with ELISA. The histological assessment was used to analyze the saffron extract effect on histopathological alterations. Saffron treatment significantly decreased osteoarthritis-associated joint histological manifestations and decreased serum TNFα levels. The flow-cytometry analysis showed a decrease in pro-inflammatory immune cell subtypes in the spleen. The results obtained suggest that saffron affected the disease progression and could be a potential therapeutic approach in osteoarthritic patients' therapy.

Optimization of a Solid-Phase Extraction Procedure for the Separation of Picrocrocin and Crocins from Saffron Extract

Saffron is a spice derived from the flower of Crocus sativus used as a flavoring and coloring agent in the food industry which also possesses medicinal properties. In the current study, the optimum Solid Phase Extraction (SPE) conditions for separating picrocrocin and crocins from aqueous saffron extracts were investigated, using Rotatable-Central Composite Design (RCCD) in combination with Response Surface Methodology (RSM). The optimized factors were volume of saffron extract (3 mL), elution solvent (15% v/v ACN/water for picrocrocin; and 50% v/v ACN/water for crocins), and volume of elution solvent (ACN/water) (10 mL). The response factor measured was the UV-Vis absorbance. The presence of picrocrocin and crocins in the solutions obtained from SPE was confirmed using Ultra-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UPLC-QToF-MS). The above optimized SPE procedure provides economy in reagents and consumables.

PH-responsive co-delivery of doxorubicin and saffron via cross-linked chitosan/laponite RD nanoparticles for enhanced-chemotherapy

Combining natural antioxidants with synthetic anticancer agents is a helpful approach in chemotherapy to reduce side effects and enhance the effectiveness of drugs. Saffron extract (SAF), a natural antioxidant with anti-cancer properties, bears a protective effect against doxorubicin-induced toxicity. Co-delivery of doxorubicin (DOX), a known anticancer agent, and SAF using nanotechnology, can be helpful to achieve a highly effective chemotherapy result. This study aimed to design cross-linked chitosan/laponite RD (CS/LP®) nanoparticles for co-encapsulation of DOX and SAF. The results indicated a high encapsulation efficiency of DOX (≥83%) and SAF (≥60%) for synthesized nanocarriers. These carriers exhibited a highly controllable pH-responsive release behavior. The release of DOX and SAF remarkably increased by decreasing pH from neutral (pH 7.4) to acidic (pH 5.5). The effects of cross-linked CS content were evaluated on the release properties. An increase in cross-linked CS content imposed a sustained release behavior by decreasing the release rate. The kinetic studies were used to confirm the release mechanisms. In vitro tests performed high cytotoxicity for doxorubicin/saffron-loaded nanoparticles against MDA-MB-231 cells. The MTT assay demonstrated no cytotoxicity for CS/LP® nanoparticles confirming its biocompatibility. The DOX/SAF-loaded nanocarriers exhibited antibacterial activity against Gram-negative and Gram-positive bacteria. • Doxorubicin and saffron were successfully co-encapsulated into cross-linked CS/LP® with high encapsulation efficiency. • The cross-linked CS/LP® nanoparticles showed simultaneous release for doxorubicin and saffron with high pH-responsive and controllable properties. • Increasing cross-linked CS content endowed sustained release by a remarkable reduction of release rate. • In vitro tests performed high cytotoxicity for doxorubicin/saffron-loaded nanoparticles against breast cancer cell lines.

Study of the dyeing properties of saffron and ultrafiltrated saffron powders, as colourants for natural and synthetic fibres

AbstractThere is a noticeable growing interest in the use of natural dyes in contemporary textile dyeing, motivated by the requirements imposed, in favour of environmental prosperity. The use of natural dyes reduces significantly both the energy requirements and the environmental impact of the process. In the present work natural and synthetic fibres were dyed with the dyestuff isolated from Crocus sativus L., after aqueous extraction of the dried stigmas of the plant. Additionally, part of the powder was purified by using ultrafiltration technology. The saffron extract and the ultrafiltrated saffron retentate were used to dye cotton, wool, nylon and polyester, in various depths of shade and temperatures. Both saffron and ultrafiltrated saffron successfully dyed not only the natural substrates, but also the synthetic ones, while higher dyeing temperatures produced level dyeing with all substrates used. Ultrafiltrated saffron powder produced brighter and much stronger dyeing to the original saffron powder, due to the elimination of extraction by‐products. Isothermal adsorptions for both colourants were performed on all substrates in order to investigate their adsorption mechanism. It was found that saffron and ultrafiltrated saffron follow a Freundlich‐type adsorption isotherm on cotton, wool and nylon which is a typical mechanism for a planar‐directed dye of big molecular weight. Nernst‐type adsorption was found to occur on polyester which again is typical for the adsorption of disperse dyes on polyester. Thus, saffron can be claimed as a universal dye, able to successfully dye natural and synthetic substrates.

A Picrocrocin-Enriched Fraction from a Saffron Extract Affects Lipid Homeostasis in HepG2 Cells through a Non-Statin-like Mode.

Dyslipidemia is a lipid metabolism disorder associated with the loss of the physiological homeostasis that ensures safe levels of lipids in the organism. This metabolic disorder can trigger pathological conditions such as atherosclerosis and cardiovascular diseases. In this regard, statins currently represent the main pharmacological therapy, but their contraindications and side effects limit their use. This is stimulating the search for new therapeutic strategies. In this work, we investigated in HepG2 cells the hypolipidemic potential of a picrocrocin-enriched fraction, analyzed by high-resolution 1H NMR and obtained from a saffron extract, the stigmas of Crocus sativus L., a precious spice that has already displayed interesting biological properties. Spectrophotometric assays, as well as expression level of the main enzymes involved in lipid metabolism, have highlighted the interesting hypolipidemic effects of this natural compound; they seem to be exerted through a non-statin-like mechanism. Overall, this work provides new insights into the metabolic effects of picrocrocin, thus confirming the biological potential of saffron and paving the way for in vivo studies that could validate this spice or its phytocomplexes as useful adjuvants in balancing blood lipid homeostasis.

Optimization of a Solid-Phase Extraction Procedure for the Separation of Picrocrocin and Crocins from Saffron Extract

Optimization of a Solid-Phase Extraction Procedure for the Separation of Picrocrocin and Crocins from Saffron Extract

Evaluation of saffron extract bioactivities relevant to skin resilience

IntroductionSaffron, Crocus sativus L, is a perennial spice herb. It has been extensively studied for its antioxidant, antidepressant, and anti-inflammatory properties, and has recently gained new interest for use in high-end cosmetics. The present work aims to elucidate the skin-protective properties of saffron in human dermal fibroblasts. MethodsThe skin-protective properties of saffron extract were evaluated in terms of tyrosinase and collagenase inhibition activities, antioxidant activity in mouse macrophage cells, collagen synthesis and hyaluronic acid synthesis and cell migration activity in primary dermal fibroblast normal human neonatal cells (HDFn). ResultsSaffron’s main phytoactive constituents - crocins, picrocrocin, safranal, and crocetin - were quantified by LC-MS at 91.0, 61.5, 3.6, and 1.9 mg/g, respectively. Saffron extract inhibited tyrosinase and collagenase with IC50 0.78 mg/mL and 0.1 mg/mL, respectively. Saffron extracts (100–200 μg/mg) suppressed reactive oxygen species and nitric oxide generation in lipopolysaccharide-stimulated RAW 264.7 murine macrophage cells and promoted collagen and hyaluronic acid synthesis in HDFn. Saffron extract at 25 μg/mg significantly promoted migration of HDFn cells (wound healing capacity) compared to control (no treatment). ConclusionThe findings highlighted the potential benefits of saffron extract relevant to skin resilience.

Fabrication of Nutraceutical Beverage from Saffron (Crocus sativus L.) Extract and Studying Its Health Effects

A saffron extract-based beverage (SEBB) was formulated and characterized based on its sensory attributes and health benefits. The main bioactive compounds of saffron extract (crocin and safranal) were quantified. Three formulations of SEBB were prepared based on the sucrose concentration: SEBB 1 contained 65 g of sucrose per 500 ml, SEBB 2 contained 17.5 g, and SEBB 3 contained 79.5 g. The SEBB most desired by consumers was then subjected to biochemical analysis to evaluate its antioxidative effects on the damage induced by food contaminated with carbon tetrachloride (CCl4). Fifteen albino rats were split into five groups and treated with different doses of CCl4 or SEBB according to the planned animal experiment for 62 days. Sensory evaluation illustrated that SEBB 1 had the highest acceptability scores. The content of crocin and safranal was 23.039 and 4.135 ppm, respectively. The SEBB ameliorated the increased activity of enzymes involved in liver and kidney function and improved the total antioxidant capacity, blood glucose, and lipid profile.

Bioactive compounds in the nutrition of peri- and postmenopausal women within the concept of 4P-medicine

An updated consensus statement from the European Menopause and Andropause Society noted that a woman's quality of life may decline during peri- and post-menopause as a result of various menopausal symptoms and age-related comorbidities. The purpose of the work was to assess the possibility of using bioactive compounds in periand postmenopausal women to prevent the development of menopausal complications and chronic diseases associated with aging. Material and methods. Literature search was carried out using PubMed, Google Scholar, ResearchGate, RSCI databases mainly over the past 10 years, with the exception of works of fundamental importance, according to the keywords "perimenopause", "postmenopause", "Affron® saffron extract", "special extract ERr 731". Results. The use of diet, dietary supplements including those containing extracts of saffron (Affron®) and Siberian rhubarb (ERr 731) and their biologically active substances, has a versatile effect on the correction of menopausal disorders due to the impact on various pathogenetic pathways of their development and can be used in women in this period as a phytonutrient support. Conclusion. The combined use of saffron and Siberian rhubarb extracts is promising from the point of view of correcting the female state during peri- and postmenopause, taking into account the pathogenetic moments of the development of menopausal complications and chronic diseases associated with aging.