The gap acceptance behavior of drivers at U-turn median openings is studied by considering the critical gap which cannot be obtained directly by field measurements. A thorough investigation on critical gap estimation is carried out by collecting the data sets from median openings at 4-, 6-, and 8-lane roads of Hyderabad City, India. Wide difference (10–42%) among the critical gap values estimated by the application of existing methods shows the limitation to consider for the mixed traffic situations. To address this issue, recently developed method Influence Area for Gap Acceptance (INAFOGA) which is based on clearing behavior of drivers at unsignalised intersections is modified and applied considering the merging behaviour of U-turn vehicles at median openings and named ‘Modified INAFOGA’ method. Modified INAFOGA method is compared with probability equilibrium method through paired-sample hypothesis (t test), and result revealed that difference in mean values 0.009 signifies that both methods are comparable. Difference in critical gap values obtained from the box plots and radar charts indicates that probability equilibrium method is not suitable to address the behavior of U-turn vehicles at median openings under mixed traffic conditions. These observations coupled with higher critical gap values validate the fact that ‘modified INAFOGA’ method is indeed appropriate under mixed traffic conditions.