Mitochondria are energy processing organelles in most cell types, essential for maintaining major cellular functions by ATP production, such as the cell‐to‐cell signaling of neurons in the mammalian nervous system. Inner ear hair cells, like all cells, use the mitochondrial machinery for their normal functioning (Song et al., 2013). The structure of these organelles, consequently, has implications for the healthy function of inner ear hair cells and impairments are the cause of certain forms of deafness and imbalance. As a double‐membraned organelle, the inner membranes of mitochondria includes the inner mitochondrial membrane and the cristae. Electron microscopic tomography (EMT) reveal that these two inner portions of mitochondria articulate with each other via narrow tubular‐shaped openings known as crista junctions (CJs) (Song et al., 2013). These structures are probably dynamic, with CJs forming and disintegrating in response to environmental changes, mitochondrial shape changes, inner membrane surface area variations or nutrient transport to the mitochondrion (Perkins and Ellisman, 2007). For this study, we predicted an asymmetrical distribution of crista junctions in mitochondria with tubular cristae located in the calyxplasm of a vestibular calyx ending adjacent to a post‐synaptic density at a ribbon synapse in a vestibular hair cell. Synaptic ribbons are the form that synapses take in retinal photoreceptors and inner ear hair cells, consisting of synaptic vesicles and an electron‐dense “ribbon” or docking site, and they are the location of vesicle fusion during active neurotransmitter release (Schmitz et al., 1996). Using the EM tomograms and 3‐D imaging software called IMOD, qualitative and computational data can be obtained on these mitochondria including their tubular cristae and CJ numbers, shape, distribution and even the density of the matrix (Tasel et al., 2016). With these tools, we hypothesized that a statistically significantly larger number of CJs will appear on the sides of mitochondria near the ribbon synapse, as opposed to away from it. Furthermore, we expect metabolic activity at the synapse to also be higher, as ATP is required for signaling activity of the ribbon synapse provided by the tubular cristae (Perkins and Ellisman, 2007). Calculations, with the help of the IMOD software has helped us bear out these assumptions, using information on the number, total volume and other details on CJs of vestibular hair cells (Tasel et al., 2016) (Song et al., 2013).Support or Funding InformationSupported by NIH R21‐DC013181 (AL) and P41‐RR004050 (GP, ME).