The creep fracture toughness of materials is required for the assessment of defective components at high-temperatures. Existing evidence indicates that creep crack-tip constraint state has a great influence on the fracture behavior of materials. In this work, the effect of constraint on creep fracture toughness was explored by conducting a series of creep crack growth tests on specimens with various thicknesses and loading modes. Results showed that the curves of creep fracture toughness with creep crack initiation time on the logarithmic scale for different groups of specimens were almost parallel with one another. Through the characterization of crack-tip constraint level for different groups of specimens in terms of a load-independent creep fracture constraint parameter, R*, the creep fracture toughness was found to increase with the decrease in the crack-tip constraint level. Moreover, evident effect of specimen geometry on creep fracture toughness was observed. Of all types of specimens, the CCT2 and CT10-SG exhibited the highest and lowest values of Kcmat at the same ti, respectively. In addition, the Kcmat was found to increase following a specimen group order of CT10-SG, CT10, CT5, SENT5, CT2, SENT2 and CCT2 at a given ti. Finally, a constraint-dependent creep fracture toughness equation was established for the Cr-Mo-V steel, which is expected for practical applications to improve accuracy of life assessment methods. Follow-up work on life assessment methods incorporating the constraint parameter R* is still necessary.