Creatine Kinase Concentrations Research Articles

Flavonoid Containing Polyphenol Consumption and Recovery from Exercise-Induced Muscle Damage: A Systematic Review and Meta-Analysis

Flavonoid polyphenols are bioactive phytochemicals found in fruits and teas among other sources. It has been postulated that foods and supplements containing flavonoid polyphenols may enhance recovery from exercise-induced muscle damage (EIMD) through upregulation of cell signalling stress response pathways, particularly the nuclear factor erythroid 2-related factor 2 (NRF2) pathway. This study aims to investigate the ability of polyphenol treatments containing flavonoids to enhance recovery of skeletal muscle strength, soreness and creatine kinase post EIMD. Medline (Pubmed), Embase and SPORTdiscus were searched from inception to August 2020 for randomised placebo-controlled trials which assessed the impact of 6 or more days of flavonoid containing polyphenol ingestion on skeletal muscle recovery in the 96-h period following a single bout of EIMD. A total of 2983 studies were screened in duplicate resulting in 26 studies included for analysis. All meta-analyses were undertaken using a random-effects model. The pooled results of these meta-analyses show flavonoid polyphenol treatments can enhance recovery of muscle strength by 7.14% (95% CI [5.50-8.78], P < 0.00001) and reduce muscle soreness by 4.12% (95% CI [-5.82 to -2.41] P = 0.00001), no change in the recovery of creatine kinase concentrations was observed. These results indicate that ingestion of polyphenol treatments which contain flavonoids has significant potential to improve recovery of muscular strength and reduce muscle soreness in the 4-day period post EIMD. However, the characterisation of polyphenol dosage and composition of study treatments should be prioritised in future research to facilitate the development of specific guidelines for the inclusion of flavonoid-rich foods in the diet of athletes and active individuals.

The Effect of Protein Supplementation versus Carbohydrate Supplementation on Muscle Damage Markers and Soreness Following a 15-km Road Race: A Double-Blind Randomized Controlled Trial.

We assessed whether a protein supplementation protocol could attenuate running-induced muscle soreness and other muscle damage markers compared to iso-caloric placebo supplementation. A double-blind randomized controlled trial was performed among 323 recreational runners (age 44 ± 11 years, 56% men) participating in a 15-km road race. Participants received milk protein or carbohydrate supplementation, for three consecutive days post-race. Habitual protein intake was assessed using 24 h recalls. Race characteristics were determined and muscle soreness was assessed with the Brief Pain Inventory at baseline and 1–3 days post-race. In a subgroup (n = 149) muscle soreness was measured with a strain gauge algometer and creatine kinase (CK) and lactate dehydrogenase (LDH) concentrations were measured. At baseline, no group-differences were observed for habitual protein intake (protein group: 79.9 ± 26.5 g/d versus placebo group: 82.0 ± 26.8 g/d, p = 0.49) and muscle soreness (protein: 0.45 ± 1.08 versus placebo: 0.44 ± 1.14, p = 0.96). Subjects completed the race with a running speed of 12 ± 2 km/h. With the Intention-to-Treat analysis no between-group differences were observed in reported muscle soreness. With the per-protocol analysis, however, the protein group reported higher muscle soreness 24 h post-race compared to the placebo group (2.96 ± 2.27 versus 2.46 ± 2.38, p = 0.039) and a lower pressure muscle pain threshold in the protein group compared to the placebo group (71.8 ± 30.0 N versus 83.9 ± 27.9 N, p = 0.019). No differences were found in concentrations of CK and LDH post-race between groups. Post-exercise protein supplementation is not more preferable than carbohydrate supplementation to reduce muscle soreness or other damage markers in recreational athletes with mostly a sufficient baseline protein intake running a 15-km road race.

Effect of Kinesio taping on delayed-onset muscle soreness in elite athletes.

This research analyzes the effect of Kinesio taping on delayed-onset muscle soreness in elite athletes because delayed-onset muscle soreness increases the risk of injury in sports. Elite female volleyball players (N.=31) were randomly divided into experimental (N.=16, with Kinesio taping of the knee) and control (N.=15) groups. To elicit delayed-onset soreness in knee joint muscles, the protocol that we used consisting of downhill running, visual analog pain score assessment, and measurements of serum interleukin-6 and creatine kinase concentrations immediately and after 24 hours, 48 hours, and 72 hours. Compared with the control group, the pain reported by the athletes with Kinesio taping decreased 24 hours and 48 hours (P<0.05) after running, but there was no significant difference 72 hours (P<0.05) after running. Interleukin-6 concentrations were lower in the athletes with Kinesio taping than those in the control group immediately and 24 hours (P<0.05) after running, but there was no significant difference 48 hours and 72 hours (P<0.05) after running. There were no significant differences (P>0.05) in creatine kinase concentrations. The use of Kinesio taping in elite athletes is an effective protective strategy for alleviating pain symptoms caused by delayed-onset muscle soreness, but it is not effective to alleviate the skeletal muscle microinjury caused by delayed-onset muscle soreness.

The effect of acute and repeated ischemic preconditioning on recovery following exercise-induced muscle damage

ObjectivesThe aim of this investigation was to determine if acute or repeated applications of ischemic preconditioning (IPC) could enhance the recovery process, following exercise induced muscle damage (EIMD). DesignRandomized control trial. MethodsTwenty-three healthy males were familiarised with the muscle damaging protocol (five sets of 20 drop jumps from a 0.6 m box) and randomly allocated to one of three groups: SHAM (3 × 5 min at 20 mmHg), Acute IPC (3 × 5 min at 220 mmHg) and Repeated IPC (3 days x 3 × 5 min at 220 mmHg). The indices of muscle damage measured included creatine kinase concentration ([CK]), thigh swelling, delayed onset muscle soreness, counter movement jumps (CMJ) and maximal voluntary isometric contraction (MVIC). ResultsBoth acute and repeated IPC improved recovery in MVIC versus SHAM. Repeated IPC led to a faster MVIC recovery at 48 h (101.5%) relative to acute IPC (92.6%) and SHAM (84.4%) (P < 0.05). Less swelling was found for both acute and repeated IPC vs. SHAM (P < 0.05) but no group effects were found for CMJ, soreness or [CK] responses (P > 0.05). ConclusionTaken together, repeated IPC can enhance recovery time of MVIC more than an acute application, and both reduce swelling following EIMD, relative to a SHAM condition.

Correlations between clinical and laboratory findings and prognostically unfavorable CMR-based characteristics of acute ST-elevation myocardial infarction.

Aim To evaluate factors associated with unfavorable predictive characteristics of ST-segment elevation acute myocardial infarction (STEMI) as per data of magnetic resonance imaging (MRI).Material and methods The study included 52 patients with STEMI who underwent a primary percutaneous coronary intervention (pPCI). Contrast-enhanced cardiac MRI was performed for all patients on days 3-7. Delayed contrast-enhancement images were used for assessing infarct size, presence of microvascular obstruction (MVO) areas, and heterogeneity zones.Results Multifactorial analysis showed that independent predictors of MVO were type 2 diabetes mellitus (DM) (relative risk (RR) 1.9, confidence interval (CI): 1.1-3.26, р=0.012), increased levels of brain natriuretic peptide (BNP) (RR 2.04, CI: 1.39-2.99, р=0.004) and creatine kinase (CK) (RR 2.06, CI: 0.52-0.80, р=0.02), and infarct size (IS) (RR 2.81; CI: 1.38-5.72, р=0.0004). Construction of ROC curves provided the quantitative values of study indexes, at which the risk of MVO increased. For BNP, this value was ≥276 pg/ml (sensitivity, 95.7 %; specificity, 37.9 %); for CK ≥160 U/l (sensitivity, 74.1 %; specificity, 61.9 %); and for IS ≥18.8 % (sensitivity, 79.3 %; specificity, 69.6 %). Correlation analysis of risk factors for increased size of the heterogeneity zone showed significant correlations of the heterogeneity zone size with older age of patients (r=0.544, р&lt;0.0001), higher concentrations of BNP (r=0.612, р&lt;0.0001), CK (r=0.3, 95 % CI: 0.02-0.5, р=0.03), and C-reactive protein (CRP) (r=0.59, CI: 0.3-0.7, р=0.0001). Increased levels of CK (r=0.53, 95 % CI: 0.29-0.70, р=0.0001) and BNP (r=0.55, 95 % CI: 0.28-0.70, p=0.0003) significantly correlated with increased IS.Conclusion Risk of MVO formation as per MRI data increased in the presence of type 2 DM and IS ≥18.8 % (р&lt;0.05). Formation of MVO in patients with STEMI was associated with increased levels of BNP ≥276 pg/ml and CK ≥160 U/l (р&lt;0.05). Increased levels of BNP, CK, and CRP were associated with a larger size of heterogeneity zone according to data of the correlation analysis. A larger heterogeneity zone was more typical for older patients. Increased levels of CK and BNP were also associated with larger IS. The correlation analysis did not show any significant interactions between the size of heterogeneity zone, IS, and MVO size (р&gt;0.05).

Seasonality of the stress response in goat bucks when there is use of electroejaculation for semen collection

Electroejaculation (EE) is frequently used to collect semen, but this procedure is both stressful and painful. In seasonal-breeding species the sensitivity to stressors might vary with season of the year when semen collection occurs, therefore, the present study was conducted to compare the responses when imposing the EE procedure, stress response to EE and fresh semen characteristics in goat bucks throughout the year. Semen was collected using EE from goat bucks once in autumn (mid-breeding season), winter (transition to the non-breeding season), spring (non-breeding season), and summer (beginning of the breeding season). The number of pulses applied during ejaculation was larger in the autumn and spring than winter and summer months. The number of vocalizations/pulse while bucks were ejaculating was greater in the summer than winter and spring months. The increase in heart rate was greater in spring than summer months. Cortisol concentration was least in the autumn, and concentration of creatine kinase (CK) was greatest in summer. Sperm mass motility was greater in autumn than spring months. Spermatozoa concentration, total number of spermatozoa ejaculated, motile spermatozoa ejaculated, and progressively motile spermatozoa were greater in the autumn and spring than in the winter and summer months. The bucks were less stressed by imposing EE procedures in the autumn, and buck responses were greater in the spring-summer period. Bucks had a greater incremental increase in serum CK and number of vocalizations in the summer months, indicating there was a greater muscular damage and quite likely pain associated with lesser semen quality.

Diagnostic and Pharmacological Potency of Creatine in Post-Viral Fatigue Syndrome.

Post-viral fatigue syndrome (PVFS) is a widespread chronic neurological disease with no definite etiological factor(s), no actual diagnostic test, and no approved pharmacological treatment, therapy, or cure. Among other features, PVFS could be accompanied by various irregularities in creatine metabolism, perturbing either tissue levels of creatine in the brain, the rates of phosphocreatine resynthesis in the skeletal muscle, or the concentrations of the enzyme creatine kinase in the blood. Furthermore, supplemental creatine and related guanidino compounds appear to impact both patient- and clinician-reported outcomes in syndromes and maladies with chronic fatigue. This paper critically overviews the most common disturbances in creatine metabolism in various PVFS populations, summarizes human trials on dietary creatine and creatine analogs in the syndrome, and discusses new frontiers and open questions for using creatine in a post-COVID-19 world.

Repercussions of pathway inhibition response of Panaxginseng fractions ameliorate cardiac dysfunction in hypertensive model rats

This study examined the preventive and therapeutic benefits of various Panax ginseng fractions against NG-nitro-L-arginine methyl ester (L-NAME)-induced hypertension in rats. Rats injected with L-NAME plus vehicle exhibited persistently elevated SBP, serum concentrations of the cardiac injury biomarkers creatine kinase (CK), CK-MB, and troponin, total cholesterol, triglyceride (TG), low-density lipoprotein (LDL), and interleukin (IL)-6, but lower high-density lipoprotein (HDL) compared to the vehicle control group. Furthermore, L-NAME disrupted the normal histological structure and function of rat cardiac tissue. All ginseng fractions and losartan restored SBP to a normal level, reversed the changes in CK, CK-MB, troponin, total cholesterol, TG, HDL, and LDH, and preserved normal myocardial histology, with the WGF demonstrating the greatest cardioprotective and antihypertensive effects. Compounds within multiple ginseng fractions, but especially the aqueous fraction, possess potent and effective antihypertensive, antilipidemic, anti-inflammatory, and cardioprotective properties.

Does coping style affect behavioral responses and growth performance of lambs weaned at different ages?

The objective of this study was to determine the relationships between coping style (CS), and physiological, behavioral characteristics and growth performance of lambs weaned at different ages. Male Karakaş lambs (n = 75, mean age = 30 d) obtained from a breeder farm were subjected to a 3-minute open field test. Of the 75 lambs classified by hierarchical clustering on principal components’ scores on open field test behaviors, 40 were assigned to 1 of 2 groups as proactive (Pro, n = 20) or reactive (Rea, n = 20). Pro (n = 10) and Rea (n = 10) lambs were selected randomly and weaned at 45 d (D45). The remaining 20 lambs (10 Pro, 10 Rea) were weaned at 75 d (D75). Lambs from Pro and Rea groups (Pro n = 5, Rea n = 5) were randomly assigned to each pen (n = 10). Blood samples were taken from lambs just before and 20 minutes after the start of open field test and at weaning (on 0, 1, 3, 5, and 7 d after weaning) to measure serum cortisol, glucose, lactate dehydrogenase, creatine kinase, total protein, and triglyceride concentrations. There was no significant effect of CS on blood parameters measured both in open field test and that measured at weaning (P > 0.05). CS had significant effect on live weight (LW) and average daily weight gain (ADWG) (P < 0.05). Reactive lambs had greater LW and ADWG than Pro lambs (P < 0.05). Weaning age (WA) had significant effect on LW and ADWG (P < 0.05). The ADWG were greater in D75 lambs than in D45 lambs (P < 0.05). CS did not have significant effect on behavioral variables measured at weaning, except for success index values. Rea lamb had greater index values than Pro lambs (P < 0.05). WA had significant effect on behavioral variables measured at weaning and those variables were higher in D75 lambs than in D45 lambs (P < 0.05). Partial correlations between open field test behaviors and those measured at weaning were not significant (P > 0.05). There was a significant positive partial correlation between aggressive behaviors and glucose (P < 0.05). The strongest partial correlations in the network were between creatine kinase and lactate dehydrogenase (r = + 0.78, P <0.05), and stereotypic behaviors and aggressive behaviors (r = + 0.67, P <0.05). Although the behavioral responses measured in the open field test are less time consuming than are those measured in ethological field studies, they do not reflect adequately the social behavior of lambs after weaning; however, WA had significant effects on social and stereotypical behaviors; therefore, to ensure social cohesion among lambs, it is important to identify the appropriate age for weaning.

Comparative Study on Electric Fish Handling Gloves and Immersion Anesthesia for the Surgical Implantation of Transmitters in Brook Trout

AbstractAlthough the use of electric fish handling gloves (EFHGs) has been advocated as a no‐residue alternative to chemical immobilization for field studies, the impact of their use on fish welfare remains uncertain for surgical procedures. The effects of EFHGs (10–16 mV), with and without local infiltration of lidocaine (6 mg/kg), on stress and muscle integrity after the surgical implantation of an intracoelomic transmitter in adult Brook Trout Salvelinus fontinalis were compared to the effects of two immersion anesthetics (buffered tricaine methanesulfonate at 60 mg/L and eugenol at 40 mg/L) using blood markers. Lower plane of anesthesia and inconsistent immobilization made surgeries with EFHGs more challenging to perform than surgeries conducted using immersion anesthesia. Plasma cortisol and lactate levels were significantly higher at 1–2 h postprocedure than at the end of the surgery (time 0) for all experimental groups (+210% and +121%, respectively), but no significant differences were detected for the magnitude of these increases between groups. Plasma creatine kinase concentrations significantly increased at 2 h postprocedure for the EFHG treatment groups (+206%), whereas they significantly decreased over the same period for the immersion anesthesia groups (−89%). Residues of lidocaine were not detected in fillets, suggesting the absence of significant systemic diffusion. This study suggests that the use of EFHGs for intracoelomic surgical procedures in Brook Trout induces higher disturbances to muscular physiology compared to immersion anesthesia. Differences in the level of stress, muscular anaerobic activity, or oxidative stress were not documented. Further research is needed to evaluate the analgesic properties of EFHGs and local infiltration with lidocaine.

Zone of Interaction Between the Parasite and the Host: Protein Profile of the Body Cavity Fluid of Gasterosteus aculeatus L. Infected with the Cestode Schistocephalus solidus (Muller, 1776).

During infection, the host and the parasite "communicate" with each other through various molecules, including proteins. The aim of this study was to describe the excretory-secretory proteins from the helminth Schistocephalus solidus and its intermediate host, the three-spined stickleback Gasterosteus aculeatus L., which are likely to be involved in interactions between them. Combined samples of washes from the G. aculeatus sticklebacks cavity infected with the S. solidus, and washes from the parasite surface were used as experimental samples, while washes from the uninfected fish body cavity were used as control. The obtained samples were analyzed using mass-spectrometry nLC-MS/MS. As a result of mass-spectrometry analysis 215 proteins were identified. Comparative quantitative analysis revealed significant differences in LFQ intensity between experimental and control samples for 20 stickleback proteins. In the experimental samples, we found an increase in the content of serpins, plasminogen, angiotensin 1-10, complement component C9, and a decrease in the content of triosephosphate isomerase, creatine kinase, fructose-biphosphate aldolase, superoxide dismutase, peroxidoxin-1, homocysteine-binding and fatty acid-binding proteins, compared to uninfected fish samples. In the experimental group washes, 30 S. solidus proteins were found, including malate dehydrogenase, annexin family proteins, serpins, peptidyl-prolyl cis-trans isomerase and fatty acid-binding protein. Thus, the protein composition of washes from the helminth S. solidus surface and the body cavity of infected and uninfected stickleback G. aculeatus were studied. As a result, it was shown that various components of the immune defense system predominated in the washes of infected fish and helminths.

Effects of post-exercise recovery methods on exercise-induced hormones and blood fatigue factors: a systematic review and meta-analysis.

High-intensity exercise consumes a large amount of energy and tends to induce post-exercise fatigue. Promoting physical and psychological recovery after exercise can enable individuals to perform better in subsequent training or competitions and reduce the risk of injury. This study aims to investigate the effects of post-exercise recovery methods on exercise-induced hormones and blood fatigue factors. PubMed, Embase and Web of Science databases were queried to collect literature on the correlation between post-exercise recovery methods and the expression of exercise-induced hormones and blood fatigue factors. The search time ranged between inception to July 2020. Stata (version 15.0) was used for meta-analysis. A total of 10 studies were included, involving the data of 278 cases. Among these, 148 people were placed in the study group and assigned active post-exercise recovery measures while 130 people were placed in the control group and assigned no post-exercise recovery measures. The results of this meta-analysis showed that there was significant difference between the study group and the control group [relative risk (RR) =15.62, 95% confidence interval (CI): 3.25, 75.06, P<0.05]. The subgroup analysis on the effect of active and passive recovery on the blood lactate concentration (BLC) and creatine kinase (CK) concentration revealed that the CK concentration [standardized mean difference (SMD) =-0.76, 95% CI: -1.47, -0.04] and BLC (SMD =-1.16, 95% CI: -2.30, -0.02) were significantly lower in the study group compared with the control group. Further analysis on the effect of different post-exercise recovery methods on the BLC and CK concentrations indicated that BLC (SMD =-1.16, 95% CI: -2.30, -0.02) was significantly lower in the group with cold water immersion compared with the control group, while there was no significant difference in the changes of CK concentration. Additionally, food supplementation was shown to reduce CK concentration (SMD =-1.16, 95% CI: -4.69, 2.36). Recovery measures after high-intensity exercise can accelerate the reduction of BLC and the activity and concentration of CK, thus helping the body quickly return to a pre-exercise state.

Sevoflurane ameliorates adriamycin-induced myocardial injury in rats through the PI3K/Akt/GSK-3β pathway.

The aim of this study was to explore the effects of sevoflurane (SEV) pretreatment on Adriamycin (ADR)-induced myocardial injury through the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/glycogen synthase kinase-3β (GSK-3β) pathway. A total of 24 rats weighing 200-250 g were divided into four groups, including: control group (C group), ADR injection group (ADR group), SEV pretreatment group (ADR + SEV group) and inhibitor group (ADR + SEV + LY group). H9c2 cells were cultured in vitro and were divided into control group (C group), ADR treatment group (ADR group), and SEV pretreatment group (ADR + SEV group) and inhibitor group (ADR + SEV + LY group) as well. Next, the content of aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) in the serum was detected via Enzyme-Linked Immunosorbent Assay (ELISA). Hematoxylin-eosin (HE) staining assay was performed to determine the severity of myocardial injury. Meanwhile, the apoptosis rate of cells was detected through terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) assay. Additionally, Western blotting (WB) was employed to measure the protein expression levels of phosphorylated (p)-GSK-3β, p-PI3K, Akt and p-Akt. Compared with C group, ADR significantly increased the content of AST, LDH and CK in the serum (p<0.01), reduced protein expression levels of p-GSK-3β, p-PI3K and p-Akt (p<0.01), increased apoptosis rate (p<0.01), and induced myocardial injury. SEV pretreatment significantly alleviated the effect of ADR, manifested as significantly lowered content of AST, LDH and CK in the serum (p<0.01), distinctly elevated protein expression levels of p-GSK-3β, p-PI3K and p-Akt (p<0.01), notably reduced apoptosis rate (p<0.01), and relieved myocardial injury. LY294002 remarkably inhibited the protective effect of SEV against myocardial injury (p<0.01) CONCLUSIONS: SEV is able to prominently ameliorate ADR-induced myocardial injury by regulating the phosphorylation level of the PI3K/Akt/GSK-3β signaling pathway.

The effect of exercise during sport training on levels of salivary diagnostic markers

Introduction/Objective. The aim of this study was to determine the changes in concentrations of urea, creatinine, uric acid, proteins, aspartate aminotransferase (AST), creatine kinase (CK), and salivary amylase in saliva samples collected before, immediately after, and 30 minutes after physical activity performed during basketball and mixed martial arts (MMA) training. Methods. Twenty-two athletes, 11 basketball players and 11 MMA fighters, 18 men and four women, aged 15?24 years, participated in the study. Saliva samples were collected using sterile saliva containers (Salivette?) from all participants before training (sample 1), immediately after (sample 2), and 30 minutes after training (sample 3). The levels of all investigated biomarkers were measured spectrophotometrically using a biochemical analyzer. Results. Statistically significant differences were present among samples 1, 2, and, 3 in the concentrations of urea, AST, and CK in samples collected from MMA fighters (Friedman test). Among three samples taken from basketball players, the significant differences were not observed for the analyzed parameters. When concentrations of all diagnostic markers were compared between basketball and MMA independently for samples 1, 2, and 3, statistically significant differences (Mann?Whitney U-test) existed in concentrations of urea, uric acid, proteins, and AST. Conclusion. Based on the results of the present study, the influence of the exercise on the levels of salivary diagnostic markers, such as urea, AST, and CK, is more evident during MMA than basketball training. Saliva composition of MMA fighters and basketball players differ in terms of levels of urea, uric acid, proteins, and AST.

Effect of Massage Before or After Exercise on Delayed-Onset Muscle Soreness in a Rat Model

Background: Delayed-onset muscle soreness (DOMS), also known as tenderness of touch, refers to the pain caused by muscle mechanical stimulation, such as contraction and stretching. Chinese massage has been widely used in the treatment of sports fatigue and sports injury, but there is controversy in the efficacy. In this experiment, we established DOMS model in rats to observe the prevention and treatment effect of massage, to find the best time for intervention, and to provide scientific basis for the prevention and treatment of exercise fatigue. Methods: 130 male SD(sprague-dawley, SD) healthy rats were randomly divided into blank group, control group and massage group. Except for blank group, the other rats received DOMS model. Professionals applied kneading and twisting methods on both lower limbs of rats. The expression of IL-2, IL-6 and IL-8 in skeletal muscle of rats were determined by western blot, PCR and ELISA, and the content of serum creatine kinase was determined by ELISA. In addition, we measured the concentration of Ca2+, Ca2+-ATPase in mitochondria of skeletal muscle. The changes of skeletal muscle structure were observed by scanning electron microscopy. Results: After massage, the expression of IL-2, IL-6, IL-8 and CK decreased compared with control group (P &lt; 0.01), the expression of IL-2, IL-6 and IL-8 in post massage group was lower than that in front massage group (P &lt; 0.01), and the content of CK in front massage group was lower than that in post massage group(P &lt; 0.01). The content of Ca2+ in front massage 24, 48 and 72h group was lower than that in post massage (P &lt; 0.01), the concentration of Ca2+-ATPase in front massage 24h and 72h group was lower than that in post massage group (P &lt; 0.05). Conclusion: Massage can prevent the injury of muscle and reduce the inflammatory reaction of muscle after exercise. It can also improve the activity of Ca2+-ATPase, enhance the transport of Ca2+ by mitochondria and protect the skeletal muscle.

Nemaline Rod/Cap Myopathy Due to Novel Homozygous MYPN Mutations: The First Report from South Asia and Comprehensive Literature Review.

Background and PurposePathogenic variants in the myopalladin gene (MYPN) are known to cause mildly progressive nemaline/cap myopathy. Only nine cases have been reported in the English literature.MethodsA detailed evaluation was conducted of the clinical, muscle magnetic resonance imaging (MRI), and genetic findings of two unrelated adults with MYPN-related cap myopathy. Genetic analysis was performed using whole-exome sequencing. MRI was performed on a 1.5-T device in patient 1.ResultsTwo unrelated adults born to consanguineous parents, a 28-year-old male and a 23-year-old female, were diagnosed with pathogenic variants in MYPN that cause cap myopathy. Both patients presented with early-onset, insidiously progressive, and minimally disabling proximodistal weakness with mild ptosis, facial weakness, and bulbar symptoms. Patient 1 had a prominent foot drop from the onset. Both patients were followed up at age 30 years, at which point serum creatine kinase concentrations were minimally elevated. There were no cardiac symptoms; electrocardiograms and two-dimensional echocardiograms were normal in both patients. Muscle MRI revealed preferential involvement of the glutei, posterior thigh muscles, and anterior leg muscles. Whole-exome sequencing revealed significant homozygous splice-site variants in both of the probands, affecting intron 10 of MYPN: c.1973+1G>C (patient 1) and c.1974-2A>C (patient 2).ConclusionsThis study elaborates on two patients with homozygous MYPN pathogenic variants, presenting as slowly progressive congenital myopathy. These patients are only the tenth and eleventh cases reported in the English literature, and the first from South Asia. The clinical phenotype reiterates the mild form of nemaline rod/cap myopathy. A comprehensive literature review is presented.

Effects of branched-chain amino acid supplement on knee peak torque and indicators of muscle damage following isokinetic exercise-induced delayed onset muscle soreness

[Purpose] This study aimed to investigate the effects of branched-chain amino acid (BCAA) supplement on delayed onset muscle soreness (DOMS) by analyzing the maximum muscle strength and indicators of muscle damage.[Methods] Twelve men with majors in physical education were assigned to the BCAA group and placebo group in a double-blinded design, and repeated measurements were conducted. DOMS was induced with an isokinetic exercise. Following BCAA administration, the changes in the knee extension peak torque, flexion peak torque, aspartate aminotransferase (AST), creatine kinase (CK), and lactate dehydrogenase (LDH) concentrations were analyzed. The maximum knee muscle strength was measured at the baseline (pre-D0) following BCAA administration for 5 days before exercise (-D5, -4D, -3D, -2D, -1D). In contrast, the post-treatment measurements (D3) were recorded after BCAA administration for 3 days (post-D0, D1, D2). Blood samples were obtained before (pre-D0), immediately after (post-D0), 24 h (D1), 48 h (D2), and 72 h (D3) after the exercise to analyze the indicators of muscle strength. BCAA was administered twice daily for 8 days (5 days and 3 days before inducing DOMS and during the experimental period, respectively).[Results] There was no difference in the flexion peak torque between the groups. However, the BCAA group showed a significantly higher extension peak torque at D3 (second isokinetic exercise), compared to the placebo group (p<.05). There was no difference in AST changes between the groups. Nonetheless, the CK and LDH were significantly reduced in the BCAA group, compared to the placebo group. There was no correlation between the extension peak torque and flexion peak torque. However, the CK and LDH increased proportionately in DOMS. Moreover, their concentrations significantly increased with a decreasing peak torque (p<.01).[Conclusion] An exercise-induced DOMS results in a decrease in the peak torque and a proportional increase in the CK and LDH concentrations. Moreover, the administration of BCAA inhibits the reduction of the extension peak torque and elevation of CK and LDH concentrations. Therefore, BCAA might be administered as a supplement to maintain the muscle strength and prevent muscle damage during vigorous exercises that may induce DOMS in sports settings.

Monitoring training and recovery responses with heart rate measures during standardized warm-up in elite badminton players.

To investigate short-term training and recovery-related effects on heart rate during a standardized submaximal running test. Ten elite badminton players (7 females and 3 males) were monitored during a 12-week training period in preparation for the World Championships. Exercise heart rate (HRex) and perceived exertion were measured in response to a 5-min submaximal shuttle-run test during the morning session warm-up. This test was repeatedly performed on Mondays after 1-2 days of pronounced recovery ('recovered' state; reference condition) and on Fridays following 4 consecutive days of training ('strained' state). In addition, the serum concentration of creatine kinase and urea, perceived recovery-stress states, and jump performance were assessed before warm-up. Creatine kinase increased in the strained compared to the recovered state and the perceived recovery-stress ratings decreased and increased, respectively (range of average effects sizes: |d| = 0.93-2.90). The overall HRex was 173 bpm and the observed within-player variability (i.e., standard deviation as a coefficient of variation [CV]) was 1.3% (90% confidence interval: 1.2% to 1.5%). A linear reduction of -1.4% (-3.0% to 0.3%) was observed in HRex over the 12-week observational period. HRex was -1.5% lower (-2.2% to -0.9%) in the strained compared to the recovered state, and the standard deviation (as a CV) representing interindividual variability in this response was 0.7% (-0.6% to 1.2%). Our findings suggest that HRex measured during a standardized warm-up can be sensitive to short-term accumulation of training load, with HRex decreasing on average in response to consecutive days of training within repeated preparatory weekly microcycles. From a practical perspective, it seems advisable to determine intra-individual recovery-strain responses by repeated testing, as HRex responses may vary substantially between and within players.

The Effect of the Oral Contraceptive Cycle Phase on Exercise-Induced Muscle Damage After Eccentric Exercise in Resistance-Trained Women.

Romero-Parra, N, Rael, B, Alfaro-Magallanes, VM, Janse de Jonge, X, Cupeiro, R, and Peinado, AB; On Behalf of the IronFEMME Study Group. The effect of the oral contraceptive cycle phase on exercise-induced muscle damage after eccentric exercise in resistance-trained women. J Strength Cond Res 35(2): 353-359, 2021-To evaluate the influence of the active pill phase versus withdrawal phase of a monophasic oral contraceptive (OC) cycle on exercise-induced muscle damage and inflammation after eccentric resistance exercise. Eighteen resistance-trained female OC users (age: 25.6 ± 4.2 years, height: 162.4 ± 5.0 cm, and body mass: 58.1 ± 5.7 kg) performed an eccentric squat-based exercise during the active pill phase and withdrawal phase of their OC cycle. Muscle soreness, counter movement jump (CMJ), and blood markers of muscle damage and inflammation were evaluated before and postexercise (0, 2, 24, and 48 hours). Creatine kinase (CK) values were higher in the withdrawal (181.8 ± 89.8 U·L-1) than in the active pill phase (144.0 ± 39.7 U·L-1) (p < 0.001). The highest CK concentrations and muscle soreness values were observed 24 hours postexercise (217.9 ± 117.5 U·L-1 and 44.7 ± 19.7, respectively) compared with baseline (115.3 ± 37.4 U·L-1 and 4.4 ± 9.2, respectively; p < 0.001). In addition, a decrease in CMJ immediately postexercise (20.23 ± 4.6 cm) was observed in comparison with baseline (24.2 ± 6.1 cm), which was not yet recovered 24 hours postexercise (21.9 ± 5.9 cm; p < 0.001). No other phase or time effects were observed. An eccentric squat-based exercise session elicits muscle damage but no inflammation response in resistance-trained women. Furthermore, the highest CK concentrations observed in the withdrawal phase suggest that this phase might be more vulnerable to muscle damage and, therefore, less adequate to administer high training loads. However, the lack of differences in other muscle damage variables between OC phases does not warrant any guidance on the active pill versus withdrawal phase.

Puerarin alleviates coronary heart disease via suppressing inflammation in a rat model.

Puerarin shows inhibitory effects on inflammation in chronic heart failure (CHF), but its efficacy in coronary heart disease (CHD) remained vague. Rat CHD model was constructed, and serum parameters were determined using a blood liquid biochemical analyzer. Also, contents of creatine kinase (CK), creatine kinase MB isoenzyme (CK-MB), lactate dehydrogenase (LDH) and cardiac troponin (cTnT) were measured using colorimetry. Histological examination was conducted with Hematoxylin-Eosin (H&E) staining, and cardiac function was assessed by Echocardiography. Cell apoptosis was detected using Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Relative expressions were measured using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot as needed. In CHD rats, the levels of TC, LDL and TG and the expressions of matrix metalloproteinase-9 (MMP-9), CD40 ligand (CD40L), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) were increased while HDL level was decreased, accompanied with inflammatory cell infiltration and cardiac malfunction. Also, the contents of CK, CK-MB, LDH and cTnT, the percentage of apoptotic cells, the expressions of Bcl-2 associated X protein (Bax), cleaved Caspase-3, TNF-α, Interleukin-β (IL-β), IL-6 and Lipoprotein-associated Phospholipase A2 (Lp-PLA2) expressions and the levels of oxidized-(ox-)LDL and malondialdehyde (MDA) were upregulated, while the level of super oxidase dismutase (SOD) and the expressions of B cell lymphoma-2 (Bcl-2) and vascular endothelial growth factor (VEGF) were downregulated. However, Puerarin ameliorated the effects of CHD model construction, suppressed nuclear factor-(NF-)κB expression, and enhanced the expressions of FarnesoidXReceptor (FXR), phosphorylated-AKT (p-AKT) and phosphorylated-signal transducer and activator of transcription 3 (p-STAT3). Puerarin alleviated CHD in rats via inhibiting inflammation, providing possible method for CHD treatment.