In this article, we report the investigation of the Ediacaran and Lower Paleozoic petroleum systems using their molecular markers. The cratonic basins located in western China were selected as a case study, whereas worldwide published samples where compiled for comparison. The study aimed in characterizing specifically and generally the molecular markers, and providing their geological significance as well. Gas chromatography-mass spectrometry experiments (GC-MS) were applied on Ediacaran reservoir solid bitumen extracts (Dengying Formation, member-Z2dn2) and Cambrian oil/rock extracts samples respectively from Sichuan Basin and Tarim Basin. The results show a predominance of n-alkanes over isoprenoids (e.g. Pr/nC17, Ph/nC18), a noticeable absence of 25-norhopane and monoaromatic steroids among all the samples. The values of pristane to phytane ratio (Pr/Ph) fall below 0.8 for all the solid bitumen samples, whereas they are equal to 0.9, on average, for the Lower-Middle Cambrian oils and rock extracts. Relatively high values of gammacerane to C30 17α (H)-hopane ratio (G/C30H), low values of C30 17α (H)-diahopane/C30H (average = 0.09), and a V-shaped distribution pattern for the following C29–C28–C27 regular steranes with C27 > C29 > C28 and C28 < C27 < C29 respectively for Ediacaran and Cambrian samples, have been noticed as well. Moreover, the values of the following ratios: ββ/(ββ+αα) and 20S/(20S + 20R) C29 steranes, tricyclic terpanes/(tricyclic terpanes +17α-hopanes), and Ts/(Ts + Tm) fall below their respective equilibrium endpoints, notwithstanding the calculated equivalent vitrinite reflectance values range within 1.69%–2.20%. The data shows that the source rocks of all the Ediacaran and Lower-Middle Cambrian samples of the western China were deposited in an anoxic and stratified environment, with the initial organic matter showing a prevalence of type II kerogen (marine algal). The organic matter have experienced none or at least a very slight biodegradation rate, in spite that the maturity rate corresponds to post-maturity level.
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