Crataegus Oxyacantha Research Articles

Analysis of Adverse Reactions Associated with the Use of Crataegus-Containing Herbal Products

Background/Objectives: Products from various parts of Crataegus species are traditionally applied as a cardiotonic. In Europe and the USA, mainly Crataegus monogyna Jacq. (Lindm.) and Crataegus laevigata (Poir.) DC (synonym Crataegus oxyacantha L.) are used, but worldwide, other Crataegus species are also used. Phytotherapeutic preparations with a standardised content of flavonoids and/or oligomeric procyanidins are commercially available. The products are generally considered as safe and are at most associated with minor and atypical adverse reactions. The aim of this study was to critically assess the information about the safety of Crataegus-containing products in humans. Methods: A scoping review of the literature about adverse reactions associated with Crataegus-containing products was performed. Next, individual case safety reports (ICSRs) were assessed, which were included in VigiBase (the World Health Organisation’s global database of adverse event reports for medicines and vaccines) and in the database of the Netherlands Pharmacovigilance Centre Lareb. The findings are discussed in relation to the literature. Results: The scoping review yielded 23 clinical studies with single-herb and 14 with multi-herb preparations, from which only a few minor gastrointestinal and cardiac events had been reported. A total of 1527 reports from VigiBase, from 1970 to 2023, were analysed, as well as 13 reports from Lareb. The most frequently reported adverse reactions belonged to the system organ classes ‘gastrointestinal disorders’, ‘skin and subcutaneous tissue disorders’, ‘general disorders and administration site conditions’, ‘cardiac disorders’ or ‘nervous system disorders’. In 277 reports of VigiBase, a single-herb product was the only suspect for causing the adverse reaction(s). Of these, 12.6% were graded as serious. Conclusions: The results of our study provide deeper insight in the adverse reaction profile of Crataegus-containing products and should contribute to their safe application in the treatment of less severe forms of cardiac failure.

Effect of crataegus oxyacantha on high blood pressure: A randomized single-blind controlled trial

BackgroundHawthorn (Crataegus laevigata) is a traditional herbal treatment for cardiovascular diseases. It contains bioactive compounds that may have cardiovascular benefits. Despite encouraging laboratory studies, additional clinical trials are required to confirm hawthorn's efficacy in treating hypertension. MethodsThis randomized controlled trial (RCT) was conducted on patients with high blood pressure (BP) who met the inclusion criteria. A total of 110 participants were randomly assigned to either the intervention group (n=55) or the control group (n=55). The intervention group received standard antihypertensive therapy plus Crataegus drops, while the control group received only standard antihypertensive therapy. BP was measured at baseline and weeks 6, 12, and 16. Adherence to treatment was evaluated using a checklist. Data were analyzed using intention-to-treat analysis and repeated measures ANOVA (p-value=0.05). ResultsThe results indicate that at week 6, the mean systolic blood pressure (SBP) and mean arterial pressure (MAP) were significantly higher in the Crataegus group compared to the control group (p=0.02 and p=0.03, respectively). However, no significant differences were observed at weeks 12 and 16. Notably, the mean values of SBP and MAP decreased significantly in the Crataegus group at 12 and 16 weeks compared to week 6. ConclusionCrataegus may decrease SBP and MAP more significantly than diastolic blood pressure (DBP). However, except for SBP at six weeks, there were no significant differences in BP between the control and Crataegus groups. Additional research with a larger sample size is required to better understand the potential of Crataegus as a treatment for hypertension.

Elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extract additives in carboxymethyl chitosan scaffolds for osteochondral tissue engineering applications

Elderberry (<i>Sambucus nigra</i>) and hawthorn (<i>Crataegus oxyacantha</i>) extract additives in carboxymethyl chitosan scaffolds for osteochondral tissue engineering applications

Antioxidant and antihyperlipidemic effects of hawthorn extract (Crataegus oxyacantha) in broiler chickens.

One of the main problems in the poultry industry is excess body fat, and the anti-fat effect of Cratagus extract has been confirmed in several studies. The present study was carried out to investigate the effects of hawthorn extract (Crataegus oxyacantha) on growth performance, haematological variables and hepatic gene expression in broiler chickens reared at high altitude (2100m). A total of 225-day-old chicks (Ross 308) were randomly distributed into three treatments. Experimental treatments were prepared by adding 0.0, 0.2 and 0.4mL of hawthorn extract per litre of consumption of water. The results showed that weight gain and feed conversion ratio were significantly improved and abdominal fat decreased by consumption of two levels of Crateagus extract when compared to the control (p<0.05). Consumption of hawthorn extract decreased circulatory levels of malondialdehyde, triacylglycerol, total cholesterol and low-density lipoproteins cholesterol but increased ferric reducing antioxidant power and high-density lipoproteins cholesterol (p<0.05). Hawthorn extract caused an up-regulation of catalase, superoxide dismutase1, glutathione peroxidase1 and peroxisome proliferator-activated receptor alpha but reduced the expression of key lipogenic enzymes (p<0.05). Overall, consumption of 0.4mL hawthorn extract per litre of drinking water, improved growth performance, suppressed lipogenesis and enhanced antioxidant response.

Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

In recent years, people have had more expectations from the developed technology in medicine, especially in the field of orthopedics and traumatology. Tissue engineers are interested in techniques that benefit from patients’ cells and biomaterials, instead of prostheses and implants. On the other hand, researchers have begun to use various medicinal plants for regeneration and anti-cancer studies. In the present study, we aimed to produce cartilage and bone inductive scaffolds for osteochondral tissue engineering applications with the addition of hawthorn or elderberry extracts. Firstly, wet electro-spun poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) fibers were integrated with a loofah mat. Then, they were impregnated into chitosan solution with/without hawthorn or elderberry extract. Composite hydrogel scaffolds were obtained by cross-linking with 0.3% (w/v) genipin. Fabricated scaffolds had more than 90% porosity and showed swelling capacity in the range of 1500–2200%. Based on the in vitro biocompatibility analyses using mesenchymal stem cells (MSCs), all the fabricated scaffolds were found to be biocompatible by WST-1, ALP activity, and GAG content analysis. Also, histological/immunohistochemical analyses showed that hawthorn and elderberry extract addition increased MSCs proliferation and collagen type I and II positivity. Consequently, all the scaffolds showed promising features for osteochondral tissue engineering applications.Graphical abstract

Catalytic, Antioxidant, and Antifungal Applications of ZnO Nanoparticles: Facile Green Synthesis by Crataegus oxyacantha Leaf Aqueous Extract

Catalytic, Antioxidant, and Antifungal Applications of ZnO Nanoparticles: Facile Green Synthesis by Crataegus oxyacantha Leaf Aqueous Extract

The Effects of Hawthorn (Crataegus oxyacantha) Fruit Extract on Performance, Carcass and Some Serum Parameters of Broilers Reared Under Heat-Stress Conditions[2

Background: The present experiment was conducted to investigate the effect of heat stress (HS) and hawthorn (Crataegus oxyacantha) fruit extract (HFE) on performance, carcass characteristics and serum parameters in broiler chickens. Methods: Experimental treatments included two different ambient temperatures (24°C: thermoneutral conditions, or 35°C: HS conditions) and three different HFE (0, 0.2 and 0.4 ml/L) in a 2´3 factorial arrangement. A total of 300 1-day-old broiler chicks were randomly allotted 1 of 6 treatments with five replicates. A total of 300 1-day old broiler chicks were randomly allocated to 6 groups with five replications of 10 birds each. HFE supplementation had no effect on growth performance, carcass characteristics and visceral weight in stressed groups (P greater than 0.05). It was also determined that it has a lowering effect on serum malondialdehyde (MDA), free fatty acid (FFA), triacylglyceride (TAG), total lipid (TL) and low density lipoproteins (LDL) (P less than 0.05), but it has no effect on other parameters (P greater than 0.05). Result: As a result, it is thought that the addition of HFE to the drinking water of broiler chickens exposed to HS has no effect on the performance characteristics, HFE has a hypolipidemic effect and would be beneficial in order to strengthen the immune system.

Teratogen Potential Evaluation of the Aqueous and Hydroalcoholic Leaf Extracts of Crataegus oxyacantha in Pregnancy Rats.

Crataegus oxyacantha is used in the treatment of cardiovascular diseases. The aim of this study was to evaluate the transplacental genotoxicity effect of aqueous (AE) and hydroalcoholic extract (HE) of leaves C. oxyacantha in a rat model and the quantification of malondialdehyde (MDA) in the liver. Three different doses of the AE and HE of the C. oxyacantha leaf were administered orally (500, 1000 and 2000 mg/kg) to Wistar rats during 5 days through the pregnancy term (16-21 days), and sampling in rats occurred every 24 h during the last 6 days of gestation, while only one sample was taken in neonates at birth. A sample of the mother's and the neonate's liver was taken for the determination of MDA. The results show that, at the hepatic level, the evaluated doses of extracts C. oxyacantha in pregnant rats and their pups did not show cytotoxicity. However, the AE and HE generated cytotoxic and genotoxic damage in the short term. On the other hand, only the AE showed a teratogenic effect. Based on these results, the AE and HE of the C. oxyacantha leaf should not be administered during pregnancy.

Anti-Atherogenic Protection by Oligomeric Proanthocyanidins via Regulating Collagen Crosslinking Against CC Diet-Induced Atherosclerosis in Rats.

The synthesis of collagen and its turnover remained as critical determinants for the progression of atherosclerosis. During this condition, proteases secreted by SMCs and foam cells in the necrotic core degrade collagen. Growing evidences demonstrated that consumption of antioxidant rich diet is highly associated with a reduced risk of atherosclerosis. Oligomeric proanthocyanidins (OPC) have been proved to possess promising antioxidant, anti-inflammatory and cardioprotective activity, based on our previous studies. The present study aims to investigate the efficacy of OPC isolated from Crataegus oxyacantha berries as a natural collagen crosslinker and anti-atherogenic agent. Spectral studies like FTIR, ultraviolet and circular dichroism analysis confirmed the in vitro crosslinking ability of OPC with rat tail collagen when compared to the standard epigallocatechin gallate. The administration of cholesterol:cholic acid (CC) diet induces proteases-mediated collagen degradation that could result in plaque instability. Further, the CC diet fed rats showed significantly increased levels of total cholesterol and triacylglycerols which, in turn, increases the activities of collagen degrading proteases-MMPs (MMP 1, 2 and 9) and Cathepsin S and D. Upon OPC treatment, marked reduction in the lipid content, activation of proteases with concomitant increase in the mRNA levels of collagen Type I and Type III as similar to atorvastatin treatment were observed .Thus, OPC supplementation may contribute to the prevention of atherosclerotic plaque instability by acting as a natural crosslinker of collagen.

Host cell cAMP-Epac-Rap1b pathway inhibition by hawthorn extract as a potential target against Trypanosoma cruzi infection.

Although the two drugs currently available for the treatment of Chagas disease, Benznidazole and Nifurtimox, have proven to be effective in the acute phase of the disease, the 60-90-day treatment leads to high toxicity and unwanted side effects, presenting, in addition, a low efficacy in the chronic phase of the disease. For this reason, new therapies that are more effective are needed. In this regard, we have recently shown that the inhibition of the Epac-Rap1b pathway suppressed the cAMP-mediated host cell invasion by Trypanosoma cruzi. Interestingly, it has been described that vitexin, a natural flavone that protects against ischemia-reperfusion damage, acts by inhibiting the expression of Epac and Rap1 proteins. Vitexin can be found in plants of the genus Crataegus spp., traditionally known as hawthorn, which are of great interest considering their highly documented use as cardio-protectors. Pre-treating cells with an extract of Crataegus oxyacantha produced levels of T. cruzi invasion comparable to the ones observed for the commercially available Epac1-specific inhibitor, ESI-09. In addition, extract-treated cells exhibited a decrease in the activation of Rap1b, suggesting that the effects of the extract would be mediated by the inhibition of the cAMP-Epac-Rap1 signaling pathway. Using HPLC-HRMS2, we could confirm the presence of vitexin, and other flavones that could act as inhibitors of Epac/Rap1b, in the extracts of C. oxyacantha. Most significantly, when cells were treated with the extract of C. oxyacantha in conjunction with Nifurtimox, an increased modulation of invasion was observed.

A clinical study on the effectiveness of homoeopathic medicine Crataegus oxyacantha in different potencies in the management of hyperlipidemia

Haw thorn is widely used Chinese herb for treatment of gastrointestinal ailments and heart problems. Hyperlipidemia is a life threatening condition which leads to coronary artery disease if treatment is not started earlier. In allopathic system statins are the most important drug used in case of hyperlipidemia and there are known side effects for the drug. There are only few studies available in the past about the success of homoeopathic medicines in treating patients suffering from hyperlipidemia. Therefore an observational study was conducted with an objective to ascertain the effectiveness of homoeopathic medicine Crataegus oxyacantha in the management of hyperlipidaemia.Methods: An observational study was conducted at OP and IP departments of Govt Homoeopathic medical college, Thiruvananthapuram. Thirty cases suffering from hyperlipidemia that followed up for 1 year were assessed using lipid profile values.Results and Discussion: The highest number of cases were in the age group 51-55, with maximum incidence in females. Out of 30 cases studies 23 patients shows significant improvement in total cholesterol values, 26 patients shows significant improvement in triglycerides values, 22 patients shows significant improvement in LDL value, 26 patients shows significant improvement in HDL value. In the present study bad cholesterol levels were found to be decreased along with that there is improvement in good cholesterol values. The wellbeing level of the patient is good after treatment with medicine Crataegus. By using the paired t test, the calculated t value of total cholesterol, triglycerides, HD Land LDL were found to be greater than the table value ie, p

LC-MS-based lipidomic analysis of liver tissue sample from spontaneously hypertensive rats treated with extract hawthorn fruits.

At present, many experiments provide support for the cardiovascular protective effect of hawthorn (Crataegus oxyacantha) flower, leaf and fruit extracts. The aim of this study was to investigate the intervention mechanism of hawthorn fruit extract on spontaneously hypertensive rats (SHR) and its effect on their lipid metabolic pattern. After SHR was intervened by hawthorn extract (1.08 g/kg/d) for 6 weeks, the blood pressure and liver histopathology of rats were evaluated. An UHPLC-Q Extractive metabolomics approach was used to collect information on rat liver lipid metabolites, combined with multivariate data analysis to identify significantly different substances and potential biomarkers through mass spectrometry and database searches. Histomorphology of the liver was partially restored in the hawthorn-treated group. Hawthorn extract interferes with sphingolipid metabolism, glycerophospholipid metabolism and glycerolipids metabolism, improving partially disturbed metabolic pathways. This study showed that hawthorn could partially restore liver histomorphology and has anti-hypertensive effect by regulating lipid metabolism.

Indication of herbal medicines and medicinal plants by the doctors of Basic Health Units

In Brazil, the use of medicinal plants in the treatment of illnesses is influenced by indigenous, African and European cultures. Approximately 80% of the world population uses plants or their preparations in health care, making it a complementary therapy. Thus, the objective was to identify the indication made by doctors working in the Family Health Strategy for medicinal plants and/or herbal medicines. Thirty-one doctors from the Basic Health Units located in Juazeiro do Norte-CE were interviewed, using semi-structured forms. For data analysis, the Microsoft Excel 2010 program was used. 23 plant species indicated for medicinal purposes and 19 phytotherapeutic purposes were identified, and Cymbopogon citratus (DC.) Stapf, Matricaria chamomilla L., Aloe vera L. Burm.f. and Peumus boldus Molina were more representative among plants, and Valeriane® (Valeriana officinalis L.); Calman® (Passiflora incarnata L., Crataegus oxyacantha L. and Salix alba L.); Ginkgo biloba® Extract (Ginkgo biloba L.); Sintocalmy® (Passiflora incarnata L.) and Liberaflux® (Hedera helix L.) were the most representative among herbal medicines. It was found that doctors recommend plants and/or herbal medicines in the treatment of certain diseases, however further study is needed regarding their use and dosage.

Crataegus oxyacantha Extract Mitigates Diabetic Nephropathy via Oxidative Stress Regulation in Streptozotocin-Induced Zebrafish Model

Crataegus oxyacantha Extract Mitigates Diabetic Nephropathy via Oxidative Stress Regulation in Streptozotocin-Induced Zebrafish Model

Molecular Mechanisms of Hawthorn Extracts in Multiple Organs Disorders in Underlying of Diabetes: A Review.

Diabetes mellitus (DM) is one of the most important metabolic disorders associated with chronic hyperglycemia and occurs when the body cannot manage insulin secretion, insulin action, or both. Autoimmune destruction of pancreatic beta cells and insulin resistance are the major pathophysiological factors of types 1 and 2 of DM, respectively. Prolonged hyperglycemia leads to multiple organs dysfunctions, including nephropathy, neuropathy, cardiomyopathy, gastropathy, and micro- and macrovascular disorders. The basis of the metabolic abnormalities in carbohydrate, fat, and protein in diabetes is insufficient action of insulin on various target tissues. Medicinal plants are rich sources of bioactive chemical compounds with therapeutic effects. The beneficial effects of leaves, fruits, and flowers extracts of Crataegus oxyacantha, commonly called hawthorn, belonging to the Rosaceae family, are widely used as hawthorn-derived medicines. Data in this review have been collected from the scientific articles published in databases such as Science Direct, Scopus, PubMed, Web of Science, and Scientific Information Database from 2000 to 2021. Based on this review, hawthorn extracts appear both therapeutic and protective effects against diabetic-related complications in various organs through molecular mechanisms, such as decreasing triglyceride, cholesterol, very low density lipoprotein and increasing the antioxidant activity of superoxide dismutase, catalase, glutathione peroxidase, total antioxidant capacity, decreasing malondialdehyde level, and attenuating tumor necrosis factor alpha, interleukin 6 and sirtuin 1/AMP-activated protein kinase (AMPK)/nuclear factor kappa B (NF-κB) pathway and increasing the phosphorylation of glucose transporter 4, insulin receptor substrate 1, AKT and phosphoinositide 3-kinases, and attenuating blood sugar and regulation of insulin secretion, insulin resistance, and improvement of histopathological changes in pancreatic beta cells. Collectively, hawthorn can be considered as one new target for the research and development of innovative drugs for the prevention or treatment of DM and related problems.

In Vitro Antibacterial Effect of Various Berries on Listeria monocytogenes as Food Borne Patogen

Listeria monocytogenes is a food borne pathogen and causes illnesses with a high mortality rate in susceptible populations. It is often incriminated in outbreaks of human listeriosis. Increasing interest in the health benefits of various berries has led to investigation of their antibacterial activity. Causative agent can multiply at refrigerator temperatures, is resistant to disinfectants, and adheres to various surfaces. Native berries were assessed for their ability to inhibit the growth of bacteria L. monocytogenes. Extracts and powder berries – sea buckthorn (Hippophae rhamnoides L.), rosehip (Rosa canina L.), black chokeberry (Aronia melanocarpa (Michx.) Elliott), grape marc (Vitis vinifera L.) and hawthorn (Crataegus oxyacantha L.) were used. All plant materials come from the Rudi-Arionești Natural Complex in the Republic of Moldova in 2017–2019. In previous studies it has been found that sea buckthorn, rosehip, black chokeberry, and hawthorn have antimicrobial effects on pathogenic microorganisms responsible for food alteration. Bacteria showed varying susceptibilities to the berry fruits. Antimicrobial properties were evaluated using well diffusion method and broth dilution method. According to the results obtained, sea buckthorn was found to have the most pronounced effect on Listeria monocytogenes, the diameter of the growth inhibition zone being 32 mm, followed by rosehip samples 26 mm. The minimum inhibition concentration (MIC) and the minimum bactericidal (MBC) were determined.

Crataegus oxyacantha Extract as a Biostimulant to Enhance Tolerance to Salinity in Tomato Plants.

Salinity is a severe abiotic problem that has harmful impacts on agriculture. Recently, biostimulants were defined as bioprotectant materials that promote plant growth and improve productivity under various stress conditions. In this study, we investigated the effect of Crataegus oxyacantha extract as a biostimulant on tomato plants (Solanum lycopersicum) grown under salt stress. Concentrations of 20 mg/L, 30 mg/L, and 70 mg/L of C. oxyacantha extract were applied to tomato plants that were grown under salt stress. The results indicated that plants that were treated with C. oxyacantha extract had a higher ability to tolerate salt stress, as demonstrated by a significant (p < 0.05) increase in plant growth and photosynthetic pigment contents, in addition to a significant increase in tomato soluble sugars and amino acids compared to the control plants. In the stressed tomato plants, malondialdehyde increased and then decreased significantly with the different concentrations of C. oxyacantha extract. Furthermore, there was a significant improvement in the antioxidant enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), and glutathione reductase (GR) in the stressed plants, especially after treatment with 70 mg/L of the extract. Overall, our results suggest that C. oxyacantha extract could be a promising biostimulant for treating tomato plants under salinity stress.

In Vitro Anti-cancer Effect of Crataegus oxyacantha Berry Extract on Hormone Receptor Positive and Triple Negative Breast Cancers via Regulation of Canonical Wnt Signaling Pathway.

Breast cancer treatment strategy depends mainly on the receptor status. Our aim was to identify a herbal preparation, effective against breast cancer, irrespective of hormone sensitivity, and to understand its molecular mechanism. The rich antioxidant composition of hawthorn (Crataegus oxyacantha) makes it a promising anti-cancer drug candidate. Polyphenol-rich methanolic extract of C. oxyacantha berry (M.Co) was found to be cytotoxic on hormone receptor positive (MCF-7) and triple negative (MDA-MB-231) breast cancer cell lines, at a dose (75μg/ml) safe on normal cells. It could effectively inhibit tumor cell proliferation and arrest cell cycle at G1/S transition in both cell lines. Molecular targets were selected from different levels of canonical Wnt signaling pathway (such as autocrine and antagonistic ligands, receptor, effector, cytoplasmic components, downstream targets, and pathway antagonist), since they are frequently found dysregulated in all breast cancers and their aberrant activation is associated with cancer stem cell expansion. M.Co could significantly downregulate the expression of Wnt pathway agonists and upregulate that of Wnt antagonists at transcriptional and translational levels, in both cell lines. To conclude, C. oxyacantha berry extract is effective against breast cancer irrespective of its hormone dependency, and cancer growth inhibition at stem cell level can be expected.

Crataegus oxyacantha leaves extract for carbon steel protection against corrosion in 1M HCl: Characterization, electrochemical, theoretical research, and surface analysis

Plant extracts have become a source of green inhibitors that guarantee highest anti-corrosion efficiency. In the present study, we characterized the phenolic compounds of the aqueous extract from the Crataegus oxyacantha leaves (COL) by UHPLC/DAD/ESI-MS and we evaluated their inhibitory activity of carbon steel corrosion in the 1M HCl medium. The UHPLC/DAD/ESI-MS and FT-IR were used to determine of the composition of aqueous extracts from COL. For the inhibitory test of corrosion we used the weight loss study, the electrochemical study (potentiodynamic and impedance), the surface analysis (SEM-EDS, UV-Visible) and quantum chemical calculations. The results showed that COL extract contains nine phenolic compounds (flavonoids and phenolic acid). Weight loss corrosion and electrochemical tests revealed that the maximum protection efficiency of the steel achieved a 94% at 3 g/L concentration of COL extract at 298K.The values ofΔGads0, Ea, ΔHa and ΔSa demonstrate an endothermic phenomenon and controlled by physisorption process. Finally, the surface analysis confirmed that the surface metallic is protected and maybe the inhibitor molecules in COL extract form bonds with the metal surface. Theoretical study backed with the experimental results.

Comparative Evaluation of Prophylactic and Therapeutic Hypolipidemic Potential of Crataegus Oxyacantha Fruit with Atorvastatin in Murine Model of Dyslipidemia

Background: Dyslipidemia or hyperlipidemia is a consequential condition resulting from derangement in lipid profile. Standard treatment such as Atorvastatin though beneficial, causes many serious adverse effects thus an alternative like Crataegus oxyacantha (Hawthorn) with hypolipidemic potential was investigated. Aims: To evaluate prophylactic and therapeutic hypolipidemic potential of crataegus oxyacantha (hawthorn) fruit in comparison with atorvastatin in murine model of dyslipidemia through assessing physiological parameter i.e., weight and serum biochemical parameters i.e., lipid profile: TC, TGs, VLDL, LDL and HDL. Study design: This experimental study was carried out in research laboratory of Akhtar Saeed Medical and Dental College and National Health Research Centre (NHRC), Lahore Methods: A murine study of 60 days was conducted on 64 male albino Wistar rats (Age  6 weeks) divided into 8 groups with 8 rats each weighing 180-200 grams. Group 1 (healthy control) received normal diet and 2 ml of normal saline for 60 days. Group 2 (disease control) received HFD and 2 ml of normal saline for 30 days while 3-5 (prophylactic groups) were given HFD along with ethanolic extract of Crataegus oxyacantha fruit, atorvastatin and their combination in doses of 40 mg/kg OD, 80 mg/kg OD and 20+40 mg/kg OD orally respectively for the same period. 6-8 (therapeutic groups) received ethanolic extract of Crataegus oxyacantha fruit, atorvastatin and their combination respectively after induction of dyslipidemia from 30th till 60th day in the same doses as mentioned above. Each rat was weighed and fasting samples for biochemical parameters were drawn by cardiac puncture in all groups at baseline and repeated at 30th day in all groups while also at 60th day in therapeutic groups as well as healthy control group. The results were analyzed using one way ANOVA for Mean±SD, post hoc Tukey’s test for group comparison in the Graph-Pad Prism (V.5) software. A p-value ≤ 0.05 was considered as statistically significant. Study period: This study was conducted from September, 2020-November, 2020. Results: Our murine study concludes that Crataegus oxyacantha (40 mg/kg OD orally) when administered prophylactically and therapeutically shows a noteworthy hypolipidemic potential when compared with Atorvastatin (80mg/kg OD orally). Better results were obtained in prophylactic doses as well as in low dose combination with Atorvastatin (20 mg/kg + 40 mg/kg respectively). Conclusion: Results suggest that Crataegus oxyacantha can be used as alternative in treating hyperlipidemias. Keywords: Crataegus oxyacantha, Atorvastatin, Hyperlipidemia.