Demand is increasing for locally grown malt barley (Hordeum vulgare L.) in northeastern North America, driven primarily by growth in the craft beer sector. A multi-site experiment was conducted to evaluate how variety (V), seeding rate (S), and nitrogen (N) fertilizer affect malt quality in the northeast. Two barley varieties (Cerveza and Newdale), two seeding rates (200 and 400 seeds m−2), and five rates of actual applied N fertility (0, 30, 60, 90, and 120 kg ha−1) were tested at Charlottetown, PE, Canada, Ithaca, NY, US, Princeville, QC, Canada, and New Liskeard and Ottawa, ON, Canada. Basic agronomic data were collected from all environments including yield, thousand kernel weight, and hectoliter weight. Barley of suitable quality was micromalted and subjected to malt quality analysis. Both V and S resulted in small effects on malt quality, however, N had the greatest effect on most measured variables. Increased rates of N application resulted in increased yield, hectoliter weight, and thousand kernel weight but had a negative effect on most quality traits, especially with increased protein content, reduced fine extract, Kolbach index, and friability, though it increased wort β-glucans. This study shows that for most years at most sites, it is possible to achieve malt quality in the northeast; however, excessive protein and the prevalence of preharvest sprout damage are the main barriers. The results of this study have implications for increased malt barley production for the craft sector as well as potential access to commodity markets for northeastern producers.