In the field of engineering, elevated temperatures often induce thermal stress. Especially in brittle materials, the propagation of internal cracks under such thermal stress is frequently the primary cause of failure. We utilize the 3D internal laser-engraved crack technology to create internal cracks at various locations inside the half-Brazilian disk specimens. By precisely controlling the power of the heating device, we achieved temperature non-uniformity of the external heat source, resulting in uneven heating of the material’s surface. Simultaneously, by adjusting the geometry of the material and using semi-Brazilian disk specimens, we generated uneven temperature gradients and thermal capacities along different heat conduction paths. The internal cracks at different locations also affect the distribution of thermal stress in the material, further influencing the formation of the non-uniform temperature fields (NUTF). Through the above methods, we successfully conducted physical experiments on the 3D propagation of internal cracks in brittle materials under NUTF. Additionally, we utilized J-integral and the finite element method for thermal analysis to conduct thermo-mechanical coupled 3D numerical simulations. Furthermore, we employed a polarising microscope to analyze the microscopic morphology of 3D internal cracks. Finally, we revealed the mechanism of 3D internal crack propagation under NUTF. The results show that prefabricated internal cracks in group A produce “n-shaped” crack at a height of 15 mm and “u-shaped” cracks at heights of 25 mm and 35 mm. Meanwhile, in group B, the eccentric prefabricated internal cracks ultimately produce “s-shaped” cracks. Through analysis, it was determined that the differential formation occurred due to relative sliding between the upper and lower surfaces of the crack under longitudinal shear stress induced by NUTF. Group A cracks have smooth surfaces, which are mode I-II cracks. group B generated “lance-like” fractures with obvious “anti-binary tree”, which are mixed mode I-II-III cracks. These findings provide an experimental and theoretical basis for the study of 3D internal crack propagation modes under NUTF and deepen the understanding of fracture mechanics in brittle materials.