In this study the Peak Stress Method (PSM) has been applied to the fatigue assessment of the welds of an axle equipped in an off-road vehicle. In the fatigue design of welded joints, the PSM is a FE-based method to rapidly evaluate the NSIFs relevant to the opening, sliding and tearing modes by means of the linear elastic peak stresses extracted from the FE model. First, an axle, which consists of a central body and two lateral branches composed by several plates welded to a tube, has been fatigue tested under a vertical load in a test bench designed to replicate the frame-axle interface. Then, an FE model of the axle has been calibrated to simulate the behaviour observed during the fatigue test, where the reaction forces and the strains at pre-defined locations have been considered. Once the FE model has been calibrated, the PSM has been applied to the welds of the axle, taking advantage of an automatic procedure, and the crack initiation location has been estimated. Finally, the theoretical estimations have been compared with the experimental datum.