Multiple semi-elliptical cracks frequently develop at the weld toes of welded joints. These interfering multiple cracks significantly accelerate crack growth rates and reduce the fatigue life of a welded joint compared to a single crack. This study proposes a method for predicting the fatigue life of a welded joint with multiple cracks, considering weld toe amplification effect, multiple crack interference amplification effect, and crack closure effect. The method is validated through fatigue tests on Q420qFNH weathering steel welded joints. The main factors influencing the interference amplification effect are investigated, and theoretical formulas for the interference amplification factor of the stress intensity factor are derived using the finite element method and the superposition principle. The initiation, aggregation, and morphological evolution during the propagation process of multiple cracks is accurately simulated, and the fatigue life of both cruciform-welded and butt-welded joints is predicted. Additionally, a quantitative analysis is conducted to assess the impact of the number of fatigue cracks on fatigue strength. The results indicate that the interference amplification effect of cracks depends on their distance and mainly affects the surface point where the crack front is closest. The proposed fatigue life prediction method can accurately estimate the fatigue life of Q420qFNH steel welded joints considering the effect of multi-cracks. The interference and coalescence of multiple cracks significantly reduce the fatigue life of welded joints. An increase in the number of fatigue cracks notably decreases the fatigue strength.