CPD Plasma Research Articles

A comparison of factor VIII activity in cryoprecipitates prepared from ACD and CPD plasma.

In an attempt to develop a method to produce a cryoprecipitate with a predictable Factor VIII potency, several variables were studied and statistically analyzed. These included the hematocrit, age, blood group and Rh type of the donor; possible epinephrine release; the degree of lipemia, volume, pH and Factor VIII activity of the donor plasma; the volume of cryoprecipitates, its Factor VIII activity and the per cent yield; and the Factor VIII activity of the supernatant plasma. Cryoprecipitates were prepared by the method of Pool and Shannon from the plasmas of 40 random male donors, half the bloods being drawn in ACD and half in CPD. None of the variables had a significant influence on the per cent yield of activity, although the mean values obtained suggest that the per cent yield of Factor VIII activity in the cryoprecipitates prepared from CPD plasmas is higher than in those prepared from ACD plasma. Although the per cent yield of Factor VIII in cryoprecipitates prepared from CPD plasma is not significantly different from that of ACD plasma, the mean total units of Factor VIII activity is significantly higher in CPD cryoprecipitates. It also was confirmed that a higher per cent Factor VIII activity in the donor results in a relatively higher activity in the cryoprecipitate. The data indicate that if CPD plasma collected from donors having above a certain minimum per cent activity were used for cryoprecipitate production, one could be assured of having a minimum of 100 units of Factor VIII activity per bag.