Cow Dung Research Articles

From Waste to Plant Production: Cattle Dung Compost as an Alternative Nursery Substrate to Commercial Peat for Producing Lettuce Plants

The limited availability of peat-based substrates, a nonrenewable resource, and the negative environmental impacts associated with their extraction require the conservation of this resource. We assessed the use of cattle dung compost as a stand-alone nursery substrate, replacing peat substrate for the production of lettuce (Lactuca sativa L.) seedlings. A completely randomized design was employed, using cattle dung compost (C) and commercial peat (P, control treatment) in various combinations (100% P; 25% C/75% P; 50% C/50% P; 75% C/25% P; 100% C) to assess their effects on different growth parameters of lettuce. Twenty-eight parameters (e.g., seed germination, seedling survival, diameter, height, biomass growth) were assessed, corresponding to the time when the seedlings are transplanted. All treatments achieved 100% seed germination and seedling survival; indicating the successful establishment of lettuce seedlings across all tested substrates. However, seedling growth was found to be significantly influenced by substrate type. Seedling diameter was significantly increased by 10.8% in the 50% C/50% P substrate, while seedling height was significantly increased by 7.7, 6.8, and nearly 10%, respectively, within the 25% C/75% P, 50% C/50% P and 100% C substrates, relative to the control. Other substrate treatments were not significant. Similar or significantly higher values than the control were also observed for biomass growth under the tested substrates, indicating the beneficial effect of cattle dung on the initial growth of plants during the nursery phase. Cattle dung compost, used as a growing substrate, demonstrated similar or superior results to commercial peat, suggesting its potential as a viable alternative potting medium for sustainable horticulture.

Effect on Growth Performance and Nutritive Value of Cultivated Azolla filiculoides As An Alternative Feedstuff for Ruminant.

Azolla filiculoides is a tiny, free-floating aquatic fern and has a potential alternative protein and fibre source for ruminants, was investigated for its cultivation optimisation and feedstuff suitability. Study 1 was conducted to investigate the influence of different fertiliser types (control, broiler manure, sheep manure, cow manure) and concentrations (0.25 g/L-1.25 g/L) on the growth performance (fresh weight, doubling time, relative growth rate) and nutrient composition (dry matter, ash, crude protein, crude fibre, crude fat) of A. filiculoides. The optimised type of fertiliser and concentration in Study 1 were further adopted in Study 2 to evaluate the effect of different fertiliser processing methods on the growth performance, nutritive value and in vitro rumen digestibility of A. filiculoides upon cultivation. The findings in Study 1 showed that cultivation of A. filiculoides using sheep manure at the concentration of 1.00 g/L is the best resulted in the shortest doubling time (3 to 5 days) and produced fresh weight (FW), relative growth rate (RGR), crude protein (CP) and crude fibre (CF) at 132.2 g/m2, 0.32 g/g/day, 21.2% DM-1 and 14.4% DM-1, respectively. Furthermore, unprocessed sheep manure (T3) exhibited superior (p < 0.05) fresh weight, relative growth rate, nutrient composition and fibre components compared to the burned manure treatment (T2). In vitro digestibility analysis discovered that T3 achieved a 24-hour accumulated gas production of 86.9 mL DM-1, with in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD) and metabolisable energy (ME) of 82.9%, 43.7% and 5.8 MJ/kg DM, respectively. These findings suggest that Azolla filiculoides cultivation can be economically optimised using 1.00 g/L unprocessed sheep manure (fresh manure), potentially serving as a self-produced, nutritious feedstuff for ruminants.

Soil Amendment with Biochar Reduces the Magnitude of Drought Stress in Soybean

Drought stress severely affects plant growth and causes huge economic losses worldwide. The beneficial effect of biochar to improve soil properties and plant growth is well discussed nowadays. To investigate biochar’s potential for drought stress mitigation, a two-factorial pot experiment was conducted. Factor one was drought stress (100% Field capacity (FC), 70% FC, and 40% FC) and another was biochar treatment. Cow dung biochar (CDB) and poultry manure biochar (PMB) was applied at three rates (0, 4 and 8 g pot−1). The experiment was designed following a completely randomized design with four replicates. The results showed that drought deteriorated all the studied parameters whereas biochar application significantly improved all the parameters compared to control treatment. The chlorophyll concentration was maximum at the application of CDB as 8 g pot−1 followed by PMB as 8 g pot−1 at both drought conditions (70% FC and 40% FC). Biochar application significantly improved plant height at 28 and 42 days after sowing and the highest plant height was found at the application CDB as 8 g pot−1 followed by PMB as 8 g pot−1. The highest leaflet number was found at the application of CDB at a rate of 8 g pot−1. On average, the root length and biomass at 70% and 40% FC was the highest at the application of 8 g CDB pot−1 which was 28.66% and 61.59% higher, respectively over control. The application of CDB at a rate of 8 g pot−1 increased the shoot biomass by 45.35 and 69.52% compared to control at 70% FC and 40% FC, respectively. Therefore, application of biochar could be an option to reduce drought stress in plants. J Bangladesh Agril Univ 22(3): 317-325, 2024

Cow dung compost and vermicompost amendments promote soil carbon stock by enhancing labile organic carbon and residual oxidizable carbon fractions in maize field soil

AbstractEnhancing soil quality and promoting the accumulation of soil organic carbon through economically and environmentally sustainable practices are essential for modern agriculture. This study aimed to investigate the effects of various organic amendments and rotation systems on soil properties and sweet corn growth in sandy loam soil. A split‐plot field experiment was conducted over 2 years, including rotations with or without Sesbania sesban as the main plot and six fertilizer treatments: a no fertilizer control, chemical fertilizer and four organic amendments as subplots. The cropping system did not significantly influence soil properties and sweet corn growth. However, the application of soybean meal, castor meal, cow dung compost and vermicompost resulted in sweet corn yields comparable to those achieved with the chemical fertilizer treatment. Notably, the soil organic matter (SOM) content was significantly higher in the cow dung compost and vermicompost treatments compared with both the no fertilizer and chemical fertilizer treatments. Moreover, these two amendments significantly increased soil carbon stock because of significant increases in labile organic carbon (TOC400) and residual oxidizable carbon (ROC) fractions in the maize field soil. A significant positive correlation was observed between fresh ear weight with husks and several soil parameters, including electrical conductivity, available P, exchangeable K and Zn, NO3–N, NH4–N, inorganic C and N, TOC400, ROC and SOM. In conclusion, cow dung compost and vermicompost not only result in sweet corn yields comparable to those from chemical fertilizer but also significantly enhance soil carbon stock by increasing TOC400 and ROC. Therefore, they can partially substitute for chemical fertilizers in promoting sustainable agricultural production of sweet corn.

Study of nitrogen status in different planting systems and organic fertilizer doses in sandy soil

This study was conducted to determine the effect of different jajar legowo planting systems in intercropping sweet corn with peanuts, as well as the addition of several doses of organic fertilizer on soil nitrogen status and plant leaf tissue on sandy soil. The research was conducted from January to May 2023, on the south coast of Jember Regency. The study used a split-plot design; the main plot consisted of the treatment of 2:2 (A1) and 4:2 (A2) jajar legowo intercropping systems. The subplots were different doses of cow dung organic fertilizer: 10 t ha-1 (B1), 20 t ha-1 (B2), and 30 t ha-1 (B3), each repeated three times. Observational data were analyzed by ANOVA and DMRT 5%. The results of the study showed that the combination of the planting system and organic fertilizer doses applied had not been able to provide optimal microclimate and soil properties for the growth and development of sweet corn plants. The A2 planting system provides a better microclimate and plant morphology for plant N availability and absorption. The addition of organic fertilizer up to the highest dose (B3) has not been able to maintain optimal soil water and N availability for sweet corn in sandy soil.

Insights into the removal of sulfamethazine and sulfonamide-resistant bacteria from wastewater by Fe-Mn spinel oxide modified cow manure biochar activated peroxymonosulfate: A nonradical pathway regulated by enhanced adsorption and 3d orbital electron reconstruction

Effectively regulating nonradical pathways is crucial for adapting to complex water environments in sulfate radical-based advanced oxidation processes (SR-AOPs). This study prepared manganese ferrite-modified cow-manure biochar composites (BC-FM) as peroxymonosulfate activators to remove sulfamethazine (SMT) and sulfonamide-resistant bacteria (SA-ARB) from wastewater. The extensive conjugated graphitic structure in biochar induced Fe 3d orbital electron reconstruction, promoting empty orbitals formation and enhancing peroxymonosulfate inner-sphere complexation. This facilitated 100 % electron-transfer-dominated nonradical regulation. BC-FM exhibited remarkable catalytic performance, with apparent rate constant of 0.170 min−1 for SMT, inactivating ∼8.6 log of SA-ARB, and effectively blocking horizontal transfer of antibiotic resistance genes in both single and complex pollution systems (SMT/SA-ARB). Moreover, ion leaching experiments, cycling tests, real-water experiments, fluidized-bed and fixed-bed experiments demonstrated BC-FM’s potential for prolonged utilization and practical implementation. This study offers new insights into designing high-performance, environment-friendly bimetal catalysts and provides a basis for remediating organic contaminants with SR-AOPs in livestock wastewater.

The Effect Addition of Soil Amandments and PGPR (Plant Growth Promoting Rhizobacteria) on the Growth and Yield of Cotton Plants Intercropped with Corn Plants in Dry Land of North Lombok Regency of Indonesia

This research investigates the effect of soil amendments in the form of cow, form goat manures and PGPR biofertilizer on cotton plants intercropped with corn in the dry lands of North Lombok Regency. The research aims is to determine the growth and yield response of cotton plants due to the application of soil amendments and PGPR in an intercropped system with corn in dryland areas. The research was conducted from December 2023 to June 2024 in Andalan Village, Bayan District, North Lombok Regency. The method used is an experimental method with field trials. The design used was Randomized Block Design (RBD), incorporating two factors: soil amendment treatment (P) as the main plot and PGPR (K) concentration treatments as the subplot. Soil amendments consisted of three levels: P0 (no cow manure and no goat manure), P1 (20 tons/ha cow manure), P2 (20 tons/ha goat manure). PGPR concentration consisted of four levels: K0 (without PGPR), K1 (20 ml/liter PGPR application), K2 (30 ml/liter PGPR application), and K3 (40 ml/liter PGPR application). The research results indicated that the application of 20 tons/ha of goat manure (P2) produced the highest average across all observed parameters (plant height, number of leaves, branch number, stem diameter, and plant yield). Similarly, the application of 40 ml/liter PGPR produced the highest average for these observed parameters. Based on the results of the land equivalent ratio (LER) analysis, this integration system shows highly suitable and relevant to be applied in the dry lands of North Lombok.

Assessing the potential of cow dung and carpet grass as feedstock for biogas production to power a generator

This study investigates the potential of cow dung and carpet grass as feedstock for biogas production, aiming to provide a sustainable energy source for powering generators. A digester was fabricated to produce biogas from a mixture of cow dung and carpet grass under controlled anaerobic digestion conditions. The composition and energy content of the biogas were analyzed, and the biogas yield was measured over a 30-day period. Key parameters such as pH, system temperature, and total dissolved solids (TDS) were monitored to understand their influence on biogas production. Results showed that the pH values ranged from 4.1 to 5.2, which is lower than the optimal range for methanogenesis, yet biogas production was still feasible, peaking at pH 5.0. System temperature ranged from 28°C to 36.8°C, with the highest biogas yield observed at the upper end of this range. TDS levels fluctuated between 1,331 ppm and 3,060 ppm, indicating variations in the feedstock composition and microbial activity. The purified biogas successfully powered a 2.5 KVA generator for 30 minutes, demonstrating its practical application as an alternative energy source. This study highlights the potential of using cow dung and carpet grass for biogas production, contributing to sustainable energy solutions and waste management strategies.

The Effect Of Soil Treatment With Cow Manure Compost And Foliar Magnesium On Plant Growth Of Black Diyala Fig Ficus Carica L. Seedlings

The research was conducted at the research station affiliated with the Faculty of Agriculture / University of Kufa in the university city in the Najaf Governorate for the period from 1 March to 1 December 2023 to study the effect of organic fertilization with cow manure compost and foliar spraying with magnesium and their interactions on some vegetative traits of black Diyala fig seedlings. The foliar magnesium was used at four concentrations (0, 0.5, 1 or 1.5) gm L-1, and the ground addition of organic fertilizer (cow manure) was also applied at four levels (0, 5, 10 or 15) g pot-1, and their interactions. The results showed that black Diyala fig seedlings treated with cow manure compost recorded significant effect on the studied traits, as cow manure compost at 15 g pot-1 resulted in the highest increase of seedlings height 21.37 cm, stem diameter 2.07 mm, number of branches 3.30 seedling branch-1, number Leaves 10.03 leaf plant -1, and leaf area 2448.25 cm2 seedling-1. Spraying fig seedlings of the Black Diyala variety with magnesium at a concentration of 1.5 gm l-1 excelled in the amount of increase in seedling height 15.98 cm, stem diameter 1.82 mm, number of branches 3.23 seedling branch-1, and number of leaves. 10.36 plant leaves-1, average leaf area is 1899.50 cm2 seedling-1. However, best results were recorded in the interaction of foliar magnesium at 1.5 gm L-1 and organic fertilizer at 15 g pot-1 with significantly the highest increase in plant height 25.56 cm and stem diameter 2.28 mm, the number of branches is 5.28 branches, seedling-1, the number of leaves is 15.00 leaves-1, the average leaf area is 3030.00 cm2, seedling-1.

Deciphering cleaner and sustainable frontiers in scientific cow waste valorization: a review.

The forecasted global population growthis poised to create a greater exigency for livestock-derived food production,leading to a significant wastegeneration from the industrial-scalelivestockoperations, which necessitates to develop sustainable waste management solutions. The heightened demand for livestock and dairy products has driven a surge in cow waste (CW) production. While CW is typically used as organic fertilizer or solid fuel, improper disposal poses potential environmental hazards. Anaerobic digestion and composting transform CW into valuable products, such as biofuels and organic fertilizers, with the potential for electricity and heat generation, biochar production, and advanced friction materials. The CW contains essential inorganic and organic compounds vital for plant functions, including lignin, cellulose, hemicellulose, nitrogen, and minerals such as potassium, sulfur, iron, magnesium, copper, cobalt, and manganese. Additionally, the rich microbial diversity in cow dung drives the production of bioenergy carriers like biomethane and biohydrogen, promoting cost-effective energy generation and environmental sustainability. This review employs bibliometric analysis to explore the latest trends in CW applications, with a particular focus on innovative applications such as cellulose extraction, biochar production, microbial fuel cells, and nanoparticle synthesis. Itfurther evaluates the environmental impacts of these technologies and assesses their potential to advance sustainable and cleaner frontiers in the valorizationof CW.

Rejuvenation of Coastal Sandy Soils through Organics

The coastal zone represents the transition from terrestrial to marine influences and vice versa. They are predominated by sandy soil texture, and have low agricultural productivity due to their properties and are vulnerable to erosion. Effective maintenance of soil organic matter is found to be a pre-requisite to ensure soil health and crop productivity. Therefore, organics based rejuvenation methods for coastal sandy soils need to be developed. A pot culture experiment was done at Kochuveli, Industrial area, in the coastal belt of Thiruvananthapuram district, Kerala, with Amaranthus (variety: Arun) as test crop. The experiment was conducted in a completely randomized design (CRD) with 12 treatments and 3 replications. In the study, effect of different organic manures such as FYM, coirpith compost, vermicompost, city compost, biochar and thermochemical organic fertilizer (TOF), with and without lime on plant growth and yield, soil organic carbon and nutrient dynamics in the southern coastal sandy soils were studied. Soil management using organic manures had considerable impact on the growth and yield characteristics of the crop. Also the analyses of post-harvest soil for nutrient parameters showed that the highest value for organic carbon, nitrogen and potassium were recorded in soil treated with lime+TOF, while the highest value for available phosphorous was recorded in treatment lime+vermicompost. The study showed that soil test based lime application and basal application of TOF @ 25 t ha-1, top dressing with fresh cow dung slurry @ 1kg per 10 litres, along with 272.7 kg rock phosphate and 122.5 kg potassium sulphate ha-1 was the best treatment to improve the soil quality, growth and yield of amaranthus in coastal sandy soils in Thiruvananthapuram district.

Effect of different combinations of feed material of cattle dung with kitchen waste on growth rate of earthworm Eutyphoeus waltoni Michaelsen

In this study the effect of different combinations of animal dung (buffalo, cow, and goat) with kitchen wastes (vegetable wastes and banana peels) on the growth rate of Eutyphoeus waltoni during vermicomposting has been investigated. To identify the most potential feed material combination for enhancement of earthworm growth, the experiment was conducted using different combination of animal dung with kitchen waste as well as dung alone. Significant growth rate of Eutyphoeus waltoni was observed in all the binary combinations of animal dung with vegetable waste and banana peels whereas; the maximum growth rate was observed in the combination of buffalo dung and vegetable waste present in (1:1) ratio. It may be due to the balanced nutrient profile provided by the mixture, which enhances the decomposition process and creates an ideal environment for earthworm growth. The feed material combination of buffalo dung and vegetable waste in (1:1) ratio is suitable for better growth and development of earthworm Eutyphoeus waltoni which not only enhances the growth rate of earthworm but also contribute to efficient waste management by converting organic waste into nutrient-rich vermicompost.

Enhanced removal of tetracycline by vitamin C-modified cow manure biochar in water

Vitamin C (VC), due to its chemical properties, can provide more oxygen-containing functional groups such as hydroxyl groups for biochar (BC), which promotes the adsorption of tetracycline on biochar. Therefore, in this study, cow dung biochar (CDBC) was modified with VC and VC-modified CDBC (CDBC-VC) was synthesized. The modified biochar was characterized and related factors, adsorption kinetics, isotherms and adsorption mechanisms were investigated. Adsorption kinetics indicate a fast rate of adsorption. The adsorption isotherms showed that the maximum adsorption capacity was 31.72 mg/g (CDBC) and 50.90 mg/g (CDBC-VC), respectively, and the adsorption process was inhomogeneous with multiple molecular layers and the adsorbent has a higher affinity. Mechanistic studies showed that hydrogen bonding interactions, π-π electron donor-acceptor interactions, hydrophobic interactions, and electrostatic interactions were the key to the adsorption process. The analysis of adsorbent regeneration showed that CDBC-VC had good adsorption performance. CDBC and CDBC-VC showed the best performance in simulated industrial wastewater with removal rates of 78.81% and 93.69%. The adsorption mechanism was comprehensively analyzed using six machine learning models. The extreme gradient boosting model gave the best fit. Analysis of the weights of the input variables for predicting adsorption efficiency showed that the ratio of initial TC concentration to BC dosage (29.8%), specific surface area (23%), isoelectric point (8.8%), and ash content (7.7%) had a significant effect on the predicted results.

Draft genome sequence of Listeria aquatica strain SG_BD1, isolated from a cow dung sample in Bangladesh.

Here, we present the genome of Listeria aquatica strain SG_BD1, isolated from cow dung in Dhaka, Bangladesh, and assembled after Oxford Nanopore sequencing. The genome is 2,690,148 bp with 2,855 predicted coding DNA sequences, G + C content of 39.6%, and displays a putative virulence gene clpP and 9 CRISPRs.

EVALUATING SOIL AMENDMENT EFFECTS ON TOMATO YIELD AND FRUIT QUALITY

This study investigates the impact of various soil treatments on key yield components of tomato (Solanum lycopersicum) plants, including fruit diameter, length, number per plant, and weight. Eight treatments were evaluated: BK+, MgSO4, CaPh, EcoTea, Pig Manure, Cow Manure, Combined Inorganic Fertilizer, and a Control. Comprehensive statistical analyses revealed significant differences in yield parameters across treatments. CaPh, MgSO4, and the Combined Inorganic fertilizer enhanced fruit production. EcoTea promoted larger tomato lengths, while Pig Dung increased weights. Mineral composition analyses showed distinct treatment effects on sodium, potassium, calcium, magnesium, and zinc levels. Nutrient profiling indicated varied impacts on moisture, protein, ash, fiber, fat, and carbohydrate contents. Strong correlations were observed between mineral and nutrient pairs, highlighting potential synergies and antagonisms. This research provides valuable insights into optimizing soil management practices for improved tomato yield, quality, and nutritional value, contributing to sustainable agricultural development.

Growth and Yield of Lentil as Influenced by Zinc and Boron Management

A field experiment was conducted at Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh, during the period from November 2021 to March 2022 to evaluate the influence of micronutrients management on growth and yield of two lentil varieties viz., BARI Masur-8 (V1) and Binamasur-8 (V2) and 6 micronutrients management (no fertilizer - F1, recommended fertilizer dose (RFD) (40-90-40-55 Kg ha−1 of urea-TSP-MoP-Gypsum) with 10 Kg ha−1 ZnSO4 as basal - F2, RFD with zinc (Zn) as foliar spray - F3, RFD with Zn and boron (B) as foliar spray - F4, 50% RFD + 50% cow dung (5 t ha−1) - F5 and 50% RFD + 50% cow dung + Zn and B as foliar spray - F6). No significant effect had found for variety except 1000-seed weight but micronutrients management along with interactions significantly effects on all studied parameters of lentil. The higher 1000-seed weight (34.16 g) was recorded in BARI Masur-8. The highest plant height (37.09 cm), number of branches plant−1 (7), dry weight of root plant−1 (159.17 mg), dry weight of shoot plant−1 (3319.33 mg) and number of pods plant−1 (62) was observed at F6 treatment. The highest 1000-seed weight (31.68 g) were recorded at F2 treatment. The highest weight of nodules plant−1 (10.33 mg at 60 DAS), seed yield (2.62 t ha−1), straw yield (2.12 t ha−1) and biological yield (4.74 t ha−1) were found at F3 treatment. The highest plant height (38.45 cm), number of branches plant−1 (7), number of pods plant−1 (63), seed yield (2.91 t ha−1) and harvest index (58.65%) were recorded at V2F6 whereas the highest weight of nodules plant-1 (26 mg), straw yield (2.22 t ha−1) and biological yield (5.02 t ha−1) were found at V1F3 but the highest 1000-seed weight (34.87 g) at V1F1. The highest number of seeds pod−1 (1.83) was found at V2F1. So, two varieties showed similar performance on yield. Foliar application of Zn has the potentiality to increase seed yield. The variety Binamasur-8 with 50% RFD + 50% cow dung + Zn and B (F6) as foliar spray could bring maximum yield (2.91 t ha−1) of lentil followed by treatment F2, F3, and F4. Bangladesh Agron. J. 2024, 26(2): 34-40

Effect of Biochar on Composting of Cow Manure and Kitchen Waste

Composting is a common method for managing organic waste and creating nutrient-rich soil amendments. Recently, biochar, a carbon-rich material from biomass pyrolysis, has been noted for potentially improving composting. This study examines the impact of adding biochar to compost made from cow manure and kitchen waste through a controlled lab experiment. The treatments were labeled as CMX (cow manure), KWX (kitchen waste), and CMKWX (both) with X being the percentage of CM, KW, and CMKW minus that of biochar in the mixture. Key parameters such as temperature (T), pH, and electric conductivity (EC) were tracked during the composting processes, and the final composts were analyzed for total nitrogen (N), available nitrogen (AN), total phosphorus (TP), available phosphorus (AP), total potassium (TK), organic carbon (OC), calcium (Ca2+), magnesium (Mg2+), and organic matter (OM). The results showed that adding less than 10% biochar influenced composting positively. Specifically, 5% biochar amendment led to higher thermophilic temperatures (45–57 °C) and stable pH levels (6.3–8.7) compared to controls. However, biochar did not significantly enhance EC, which peaked at 1.78 dS/m in both the control and 5% biochar treatments. Nutrient analysis revealed that biochar increased Ca2+ (13.62 meq/g) and Mg2+ (5.73 meq/g) retention in CM composts (CM85 and CM100). The highest OM content was 16.84% in CM90, while the lowest was 3.81% in CM95. Higher OM negatively affected TN, with CM treatments having more OM and KW treatments having more TN. TP and TK were higher in control treatments without biochar. This study highlights the benefits of integrating biochar with organic waste for enhancing compost nutrient profiles and soil fertility. It was observed that the more diverse the compost feedstock, i.e., CMKW, the higher the nutrient content for treatments containing less than 10% biochar.

Influence of Different Cow Manure Application Rates on the Growth, Yield and Indigo Blue Color of Indigofera suffruticosa Mill

Background: Indigo is a key ingredient in the natural dyeing of fabrics. However, the conventional use of chemical fertilizers in indigo cultivation has led to an increase in production costs and soil degradation. Low-fertility soils further compromise the growth and yield of indigo plants. To address these challenges, employing manure is recognized as a sustainable approach for indigo cultivation. The use of manure enhances soil nutrient levels, improves soil structure and mitigates production costs. In this study, our objectives were to compare different rates of cow manure in relation to indigo growth, yield and the effect of cow manure rate on the value of indigo blue color. Methods: The experiments were carried out at the Plant Science Field from June to October 2022. Employing a randomized complete block design (RCBD), the study featured four treatments with four replications each. The treatments included: Treatment 1: no fertilizer (control); Treatment 2: cow manure at a rate of 3,125 kg/ha; Treatment 3: cow manure at a rate of 6,250 kg/ha; and Treatment 4: cow manure at a rate of 9,375 kg/ha. Data collection included plant height, canopy width, number of branches, plant weight, indigo blue weight and indigo blue color value. The CIE color system (Commission International de l’Éclairage) determined the color value. Result: The application of cow manure at a rate of 9,375 kg/ha resulted in the highest plant height and canopy width, with the corresponding number of branches of 150.73 cm, 46.45 cm and 31.73 cm, respectively. As compared to the other treatments, these values were statistically different. The color that was associated with the indigo finished product was dark blue and the yield; plant weight and indigo blue weight were recorded as 15,663.44 kg/ha and 2,540.44 kg/ha, respectively. The color value of indigo blue showed that the L* values of 24.94, a* -9.32, b* -2.44 and C* 9.63 were not significant but h* 194.66 was statistically significant.

Community-level effects of ivermectin and moxidectin from cattle dung: zooplankton as study case

Context Ivermectin (IVM) and moxidectin (MOX), are widely used internal and external antiparasitic drugs for livestock. They enter into the aquatic environment because the treated animals metabolise only a small percentage of the administered doses, and the rest is eliminated through the faeces posing a risk to aquatic organisms. Aims This study aimed to evaluate the responses of zooplankton to environmentally relevant concentrations of IVM and MOX spiked in cattle dung in a short-term exposure. Methods We compared the composition of the community, density, species richness, diversity and total biomass between treatments with parasiticides and controls. Key results The presence of both antiparasitic drugs altered the zooplankton community structure, leading to a shift in species composition. Cladocerans were the most affected, with a drastic reduction in their density. Additionally, a decrease in the density of copepods and an increase in the density of rotifers were observed in the MOX treatments. Conclusions Both drugs caused a simplification of the zooplankton community as species richness and diversity decreased. Overall, MOX was more toxic than IVM. Implications Our results suggest that long-term consequences on ecosystem services could arise, which merits the development of control and livestock management tools for the protection of these environments.

Effects of Organic Manure on Wheat Yield and Accumulation of Heavy Metals in a Soil—Wheat System

The application of organic manure is an effective way to develop sustainable agriculture. However, the application of organic manure may be associated with a potential risk of heavy metal pollution for soil and crops. In this study, the effects of organic cow manure (T1) (as base fertilizer), organic pig manure (T2) (as base fertilizer) and chemical fertilizer (T3) on winter wheat grain yields, grain quality, heavy metal concentrations and heavy metal bioconcentration factors (BCFs) in a soil–wheat system were studied from November 2021 to June 2023. The results showed that the winter wheat grain yields in the T1 and T2 treatments were lower than those in the T3 treatment by 2.57–38.0% and 10.5–25%, respectively. There were no significant differences in quality indexes of winter wheat grain among different fertilizer treatments. The concentrations of heavy metals in topsoil and winter wheat were 0.12–76.11 μg/g and 0.01–43.25 μg/g, respectively. The BCFs of heavy mental in the soil–wheat grain system was 0–2.92. In general, there were no significant differences in heavy metals’ concentrations in topsoil and wheat grain among different fertilizer treatments. In summary, compared with chemical fertilizer, the short-term application of organic manures had no significant effect on heavy metals concentrations in topsoil and wheat.