Cull Cows Research Articles

331 Economic comparison of march and may calving systems in the nebraska sandhillls

Abstract A 3-yr study evaluated the economic differences between a March and May calving production system of crossbred beef cows and their offspring from the Gudmundsen Sandhills Laboratory, Whitman, NE. Both herds were treated as a common when not in treatment period. Adjusted calf weaning BW was higher (P) for March Calves (226.4 ± 1.1 kg vs 193.4 ± 2 kg). Pregnancy rates (89% vs 91%) were similar through both systems. The stochastic economic model used for the analysis was based on 9 yr of USDA AMS data. The model accounts for most assumptions within each system, including all labor, cull and replacement cow costs and feed costs. This analysis was on the total calf costs, total pair feed costs and average market net return at weaning. March systems wintered on hay had a positive net return 2 out of the 9 yr (Average of -$88.76/calf) and on cornstalks 8 out of 9 years in Dawson County, NE (Average of $62.75/calf). March systems on winter range feed 0.41 kg DM/(cow • d) of supplement had a positive net return 4 out of the 9 yrs (Average of $25.23/calf). The May system, no matter the treatments of range or meadow and with or without supplement, had a positive net return 2 out of the 9 years (Average of $-65.77 and $-83.90/calf). Within this analysis, even with the input costs being higher for March, the net return was still greater in a March vs May system. Further analysis will be done on different trts within each system.

Relationships Between Cull Beef Cow Characteristics, Finishing Practices and Meat Quality Traits of Longissimus thoracis and Rectus abdominis.

The aim of study was to investigate the relationships between the characteristics of cull beef cows in the Rouge des Prés breed, finishing practices and physicochemical characteristics and sensory traits of Longissimus thoracis (LT) and Rectus abdominis (RA) muscles from 111 cows. On the basis of our surveys, which qualify at cow level the animal characteristics and finishing diet, clusters of cull cows and finishing practices are created and their effects tested on LT and RA meat quality. Old and heavy cows with good suckling ability (95 months, 466 kg and 7.1/10) are characterized by LT with larger fibers, and higher intramuscular fat content and fat-to-muscle ratio. Young and heavy cows with low suckling ability (54 months, 474 kg and 4.4/10) are characterized by LT and RA with lower MyHC IIx and higher MyHC IIa and MyHC I proportions. MyHC IIx and IIa proportions are lower and a* and b* color indices higher when cows are finished on pasture, probably related to grass diet and physical activity. The fat-to-muscle ratio is higher without any effect on the intramuscular fat content when cows are finished over a short period (107 days) with a high level of concentrate (9.7 kg/day). The opposite effect is observed over a long period (142 days) with a low level of concentrate (5.8 kg/day), confirming the interaction effect between finishing duration and amount of energy concentrate on the allotment of adipose tissue deposit.

Algicidal effect of blue light on pathogenic Prototheca species.

Prototheca spp. are pathogenic algae with important zoonotic potential. Most importantly, these algae often infect dairy cattle. Since there is no effective therapy against the algae, the standard recommendation is the disposal or culling of infected cows to avoid outbreaks. This study investigated the ability of blue light to inactivate pathogenic Prototheca species. Blue LED light (λ = 410 nm) was used to inactivate in vitro suspensions of P. zopfii genotypes 1 and 2, and P. blaschkeae. Our results showed that blue light irradiation induced a strain-specific dose-dependent algicidal effect against all tested strains. P. zopfii genotype 1, was more sensitive than genotype 2 and P. blaschkeae was the most tolerant. Even though we observed different inactivation kinetics, all strains presented significant photoinactivation levels within feasible procedure periods. Therefore, we conclude that blue light irradiation offers promising potential for the development of novel technologies that control contaminations and infections caused by Prototheca spp.

Association between herd management practices and antimicrobial resistance in Salmonella spp. from cull dairy cattle in Central California.

BackgroundIn this study cull dairy cows from six California dairy herds were sampled seasonally over the course of a year. The objectives were to determine the prevalence of antimicrobial resistant (AMR) Salmonella spp. shed in cull cow feces, and the factors associated with fecal shedding of AMR and multidrug resistant (MDR) Salmonella.MethodsSix dairy farms located in the San Joaquin Valley of California were identified and enrolled as a convenience sample. On each dairy, and once during each of the four seasons, 10 cull cows were randomly selected for fecal sampling on the day of their removal from the herd. In addition, study personnel completed a survey based on responses of the herd manager to questions related to the previous 4 month’s herd management and the specific cattle sampled. Fecal samples were submitted to the California Animal Health and Food Safety laboratory for Salmonella isolation. Antimicrobial resistance was evaluated using broth microdilution method and a gram-negative assay plate following Clinical Laboratory Standards Institute (CLSI) guidelines and breakpoint references. All statistical models were survey adjusted for number of animals on sampling day.ResultsA total of 62 Salmonella were isolated from 60 of the 239 fecal samples collected. For 12% (95% confidence interval (CI) [3–20]) of fecal samples a multidrug resistant Salmonella was isolated. The survey-weighted results for the two most common drug classes for which isolates were resistant were tetracycline (39%; 95% CI [27–51]) and ampicillin (18%; 95% CI [9–27]). An important finding was the identification of cephalosporin as the third most common drug class for which isolates were resistant, with ceftriaxone (10%; 95% CI [2–17]) being the most common drug associated with resistance in that class. At the cow-level, reason for culling, prior treatment with antimicrobial drugs as the reason for culling was associated with higher odds of isolating an AMR Salmonella isolate. At the herd-level, percent of animals monthly culled on the farm as well as number of milking cows in the herd were associated with isolation of antimicrobial resistant Salmonella in cull cows.DiscussionSalmonella isolated from fecal samples from cull cows were resistant to important antimicrobials, such as ceftriaxone. The most common drug classes for which isolates were resistant were tetracyclines and beta-lactams, with ampicillin, ceftriaxone and ceftiofur being the three most common drugs within the latter. Cow and herd level factors were associated with isolating antimicrobial resistant Salmonella that should be further investigated for their potential role in promoting occurrence of AMR Salmonella. Our results also highlight the importance of monitoring dairy cattle sent to slaughter for shedding of Salmonella resistant to medically important antimicrobial drugs.

Short communication: Survey of hepatic copper concentrations in Midwest dairy cows

Previous research from our laboratory and others indicates that liver copper concentrations in dairy cattle are commonly well above those recognized as adequate for the nutritional needs of the animal. It has also been speculated that hepatic copper concentrations have been increasing in recent years. Unlike other species, the threshold at which elevated liver copper concentrations becomes deleterious to hepatocytes is not known for cattle. Therefore, the objectives of this study were 3-fold: (1) to delineate differences in the range and mean dry matter hepatic copper concentration for dairy cattle in a retrospective analysis (January 1, 2007, to December 31, 2015), (2) to investigate hepatic copper concentrations in Midwest cull dairy cattle, and (3) to evaluate histologic changes in hepatocellular morphology in the context of copper concentration in cull cows. Furthermore, microscopic changes in hepatocellular morphology or architecture were examined and scored for evidence of inflammation, fibrosis, necrosis, and abundance of rhodanine-stained granules using hematoxylin and eosin and rhodanine staining. The retrospective analysis found copper concentrations within a range of 3 to 1,963 µg/g, with a mean of 473 µg/g. Hepatic copper concentrations in our retrospective study did not increase with time. In our abattoir analysis, copper concentrations ranged from 15 to 978 μg/g, with a mean of 390 μg/g. This study found that the range and mean hepatic copper concentrations were comparatively less in the current abattoir study than copper concentrations in our retrospective analysis. There was no evidence for hepatocellular changes associated with increased copper burdens in this study population.

Managing cow herd dynamics in environments of limited forage productivity and livestock marketing channels: An application to semi-arid Pacific island beef production using system dynamics

Sustainable ranching operations require access to adequate forage reserves and suitable means to market livestock, both of which are critical determinants of adaptive capacity (defined here as the ability to manipulate stocking rate). Ranch adaptive capacity is most relevant during times of forage shortages from drought. Unfortunately for island beef production systems, traditional adaptive measures used in continental systems are unavailable, such as transporting livestock to less affected areas, importing feed resources (cost prohibitive), intensive grazing practices or stockpiling forage (since forages mature too rapidly and are generally low quality), or destocking through cull cow sales (due to limited marketing and processing capacities). Located on an island of Hawaii, the case study ranch investigated here is challenged by each of these environmental and market constraints. The ranch resides on the leeward side of its island such that it receives minimal rainfall and forage productivity is similar to semi-arid rangelands in the western United States. The ranch's livestock management problem is compounded during drought, since island slaughter capacity is limited and there is no financially feasible means of marketing and transporting culled livestock off the island. Therefore, when forage is limited, managers are forced to retain ownership of culled mature cows, who are moved into a terminal herd to await the next available harvest or shipping availability. Terminal herds occupy areas with lower quality forages to conserve the most productive pastures for higher valued calves. This backlog of cull cows creates extended periods of stress on forage resources, since grazing pressure is not relieved as drought intensifies and increases operational expenses. A simulation model was created in an effort to identify key leverage points within the ranching operation that have the greatest impact on forage availability, herd size and net income. Upon completion of the model, sensitivity analyses were conducted to identify key drivers of model behaviors and several what-if scenarios were run based on questions provided by island ranch managers. Results showed that reducing terminal herd size through increased island processing capacity would not relieve forage pressure or eliminate the backlog of terminal cull cows, although net income was improved through greater cow sales. Several follow up tests were then run to evaluate changes to internal ranch decision making, which showed that reductions in heifer retention would provide a wider array of ecological and economic benefits. The ranch's ability to manipulate heifer retention rates, rather than cull cow rates, terminal herd shipping, or island processing capacity, was shown to be the critical aspect which drives ranch adaptive capacity.

Evaluation of national surveillance methods for detection of Irish dairy herds infected with Mycobacterium avium ssp. paratuberculosis

The aim of this study was to evaluate the utility and cost-effectiveness of a range of national surveillance methods for paratuberculosis in Irish dairy herds. We simulated alternative surveillance strategies applied to dairy cattle herds for the detection of Mycobacterium avium ssp. paratuberculosis (MAP)-infected herds (case-detection) or for estimation of confidence of herd freedom from infection (assurance testing). Strategies simulated included whole-herd milk or serum serology, serology on cull cows at slaughter, bulk milk tank serology, environmental testing, and pooled fecal testing. None of the strategies evaluated were ideal for widespread national case-detection surveillance. Herd testing with milk or serum ELISA or pooled fecal testing were the most effective methods currently available for detection of MAP-infected herds, with median herd sensitivity >60% and 100% herd specificity, although they are relatively expensive for widespread use. Environmental sampling shows promise as an alternative, with median herd sensitivity of 69%, but is also expensive unless samples can be pooled and requires further validation under Irish conditions. Bulk tank milk testing is the lowest cost option and may be useful for detecting high-prevalence herds but had median herd sensitivity <10% and positive predictive value of 85%. Cull cow sampling strategies were also lower cost but had median herd sensitivity <40% and herd positive predictive values of <50%, resulting in an increased number of test-positive herds, each of which requires follow-up herd testing to clarify status. Possible false-positive herd testing results associated with prior tuberculosis testing also presented logistical issues for both cull cow and bulk milk testing. Whole-herd milk or serum ELISA testing are currently the preferred testing strategies to estimate confidence of herd freedom from MAP in dairy herds due to the good technical performance and moderate cost of these strategies for individual herd testing. Cull cow serology and bulk tank milk sampling provide only minimal assurance value, with confidence of herd freedom increasing only minimally above the prior estimate. Different testing strategies should be considered when deciding on cost-effective approaches for case-detection compared with those used for building confidence of herd freedom (assurance testing) as part of a national program.

Culling Decisions and Dairy Cattle Welfare During Transport to Slaughter in the United States.

Nearly a third of dairy cows are removed from herds annually in the United States. Our objective is to describe what is known about the process of sending a dairy cow to slaughter in the United States including our perspectives about her fitness for transport, her condition upon arrival at the slaughter plant and the decisions to transport her in the first place. This process begins when the decision is made by the farmer to remove a cow from the herd. Once a cow leaves the farm, she makes her way either directly to slaughter or goes through one or more livestock auctions or markets along the way. Cull cows can travel considerable distance to slaughter and may face a number of welfare challenges during this process. These stressors are exacerbated if the cows are compromised and not fit for transport. While all major industry stakeholders have recommendations or guidelines about fitness for transport, none are enforced rules or regulations. There is little financial disincentive for farmers to stop shipping compromised dairy cows, and, in some cases, slaughter plants are willing to take the risk on purchasing cows in this condition as those that survive the journey often generate a good margin of return. As a result, the decision to ship compromised cull cows is too common, as indicated by data about cow condition both at the farm and the slaughter plant. Compromised culled dairy cattle continue to arrive at slaughter plants and leadership within the industry is needed to tackle this welfare challenge.

Identification of key rearing factors to manage cattle carcass fatness and conformation scores during the fattening period

The fatness and conformation scores characterise the payment of cattle carcasses in the EUROP system. The management of these carcass traits is important for stakeholders of the meat chain. The aim of this study was to identify rearing factors, applied during the fattening period, influencing the fatness and conformation scores of the carcasses, in two categories of animals (cull cows and young bulls). A data set of individual cattle data (233 cull cows and 565 young bulls) from different experiments was used. It included eleven rearing factors characterising the fattening period. From carcass data, clusters of fatness and conformation scores were defined. To identify the rearing factors with a leverage on carcass traits according to the category of animal, four robust prediction models were developed using logistic regression to predict these clusters. According to the results of the models established, to achieve the fatness score rehearsed by stakeholders, cull cows should ingest less dry matter (DM) and have a shorter fattening period, whereas young bulls should be more efficient, ingest more DM and be slaughtered heavier. To improve the carcass conformation score, the cull cows should ingest more net energy and begin their fattening heavier. For the young bulls, the three rearing factors identified for the fatness score allowed to improve the conformation score. This study showed also that the jointly management of these both carcass traits is possible, nevertheless it is necessary to adapt the applied fattening practices according to the category of animal.HighlightsUsed rearing factors to predict the fat score and conformation of bovine carcasses.Logistic regression is well adapted to predict fat score and conformation of bovine carcasses.The prediction models of the fat score and conformation are robust.

Assessment the Calf’s Welfare Due to The Gender, Number of Offspring and Calving Status in Holstein Calves

The aim of the present paper was to study the influence of gender, number of offspring, calving status on both health and welfare. Out of 3000 heads, data for 429 Holstein female calves and 551 male calves which have been born during one year were used with computer records. The result suggested that abnormal behaviour during 1st 12 hours of calf's life have been significantly affected by twinning and dystocia .The presence and duration of behaviour related to respiratory, digestive or mixed disorders were significantly higher in females, the calf’s growth rate was higher in male than female with significance difference, while its birth weight was significantly higher in dystocia than calves from normal parturition. There was a non-significant effect of calf gender, number of offspring and parturition state on calves weaning age. It could be concluded that Calf gender, number of offspring and parturition state have a different effects on behaviour and growth performance of calves from birth till weaning. Abnormal behaviours during the first 12 hours of calf's life as delayed sternal recumbancy, standing, abnormal suckling have been significantly affected by twinning and dystocia and not significantly affected by calf sex. Presence and duration of behaviour related to respiratory, digestive or mixed disorders were significantly higher in females than males, where these disorders were significantly higher in case of dystocia and twinning births. Culling of cows with incidence of twinning is recommended to control the decreased fertility, decreased total number of calves over the lifespan of the cow, and decreased milk production.

Impacts of Various Dry-Aging Methods on Meat Quality and Palatability Attributes of Beef Loins from Cull Cow

Impacts of Various Dry-Aging Methods on Meat Quality and Palatability Attributes of Beef Loins from Cull Cow

Transcriptome changes in muscle of Nellore cows submitted to recovery weight gain under grazing condition

The aim of this study was to evaluate transcriptome changes in the muscle tissue of Bos taurus indicus cull cows subjected to recovery weight gain under grazing conditions. In all, 38 Nellore cull cows were divided randomly into two different management groups: (1) Maintenance (MA) and (2) Recovery gain (RG) from weight loss by moderate growth under high forage availability. After slaughter, RNA analysis was performed on the Longissimus thoracis muscle. Semaphorin 4A, solute carrier family 11 member 1, and Ficolin-2 were expressed in the RG, which may indicate an inflammatory response during tissue regrowth. Signaling factors, such as Myostatin, related to fibroblast activation, negative control of satellite cell proliferation in adults and muscle protein synthesis were less abundant in the RG group. The only gene related to anabolic processes that were more abundant in the MA group was related to fat deposition. The genes that were differentially expressed in the experiment showed muscle repair-related changes during RG based on the greater expression of genes involved in inflammatory responses and the lower expression of negative regulators of muscle cell proliferation and hypertrophy.

ПРОДУКТИВНЫЕ КАЧЕСТВА КОРОВ ГОЛШТИНСКОЙ ПОРОДЫ РАЗНОЙ СЕЛЕКЦИИ В УСЛОВИЯХ СМОЛЕНСКОЙ ОБЛАСТИ

According to the results of work for 2018 ZAO Zolotaya Niva in the Smolensk region occupies a leading position in dairy productivity of cows. A herd of pedigree reproducer was formed in the period from 2010 to 2015 years of animals, imported from Germany, Denmark and USA. The highest productivity of cows in the herd reaches the level of 14-16 thousand kg. of milk per lactation, with a fat content of 4,32-4,42 % and protein - 3,42-3,52 %. The largest number of animals is begun to yield more milk to the level of 8-12 thsd. kg. Daughters born in the United States, Denmark and Russia were worse for signs of dairy productivity than their mothers, unlike daughters from Germany. Average values of productivity from 1 to 7 lactation have a tendency to increase. Affiliation to line of breeding, except cows from Russia, does not have a significant impact on their highest productivity. The best productive qualities have daughters born in Russia and imported from the United States, received from the bulls of American breeding. Cull cows in the herd mainly occur at 2-3 of lactation for reasons of disease of the legs, udder, genital organs and animal defects. The average life expectancy of cows on the farm is 2,8 lactation.

Estimating the impact of clinical mastitis in dairy cows on greenhouse gas emissions using a dynamic stochastic simulation model: a case study

The increasing attention for global warming is likely to contribute to the introduction of policies or other incentives to reduce greenhouse gas (GHG) emissions related to livestock production, including dairy. The dairy sector is an important contributor to GHG emissions. Clinical mastitis (CM), an intramammary infection, results in reduced milk production and fertility, increases culling and mortality of cows and, therefore, has a negative impact on the efficiency (output/input) of milk production. This may increase GHG emissions per unit of product. Our objective was to estimate the impact of CM in dairy cows on GHG emissions of milk production for the Dutch situation. A dynamic stochastic simulation model was developed to simulate the dynamics and losses of CM for individual lactations. Cows receive a parity (1 to 5+), a milk production and a calving interval (CI). Based on the parity, cows have a risk of CM, with a maximum of three cases in a lactation. Pathogens causing CM were classified as gram-positive bacteria, gram-negative bacteria, or other. Based on the parity and pathogen combinations, cows had a reduced milk production, discarded milk, prolonged CI and a risk of removal (culling and mortality) that reduce productivity of dairy cows and therefore increase GHG emissions per unit of product. Using life cycle assessment, emissions of GHGs were estimated from cradle to farm gate for processes along the milk production chain that are affected by CM. Processes included were feed production, enteric fermentation, and manure management. Emissions of GHGs were expressed as kg CO2 equivalents per ton of fat-and-protein-corrected milk (kg CO2e/t FPCM). Emissions of cows with CM increased on average by 57.5 (6.2%) kg CO2e/t FPCM compared with cows without CM. This increase was caused by removal (39%), discarded milk (38%), reduced milk production (17%) and prolonged CI (6%). The GHG emissions increased by 48 kg CO2e/t FPCM for cows with one case of CM, by 69 kg CO2e/t FPCM for cows with two cases of CM and by 92 kg CO2e/t FPCM for cows with three cases of CM compared with cows without CM. Preventing CM can be an effective strategy for farmers to reduce GHG emissions and can contribute to sustainable development of the dairy sector, because this also can improve the income of farmers and the welfare of cows. The impact of CM on GHG emissions, however, will vary between farms due to environmental conditions and management practices.

Effect of cow replacement strategy on cow and calf performance in the beef herd

Two contrasting replacement strategies are used by Irish beef farmers to select replacement females – animals sourced from within the suckler beef herd and sourced from the dairy herd. The objective of this study was to investigate the effect of replacement strategy (i.e. beef v. beef×dairy (BDX)) on cow and calf performance using data from the national beef database across a range of beef and dairy breeds. The association between replacement strategy and calving difficulty score, calving interval, weaning weight, weaning price and all carcass traits was investigated using a mixed model. The effect of replacement strategy on cow survival, calving dystocia and calf perinatal mortality was quantified using logistic regression. Beef cows were older (10.92 days; P<0.001) at their first calving, but were 1.15 times (P<0.01) more likely to survive to a subsequent lactation compared with BDX cows. Calving interval was 1.53 days shorter (P<0.001) for BDX compared with beef cows. Greater calving difficulty and calving dystocia was associated with beef cows (P<0.001) relative to BDX. However, BDX were 1.36 times (P<0.001) more likely to have a dead calf at birth relative to beef cows. Calves weaned from BDX were heavier (18.49 kg; P<0.001) at weaning, reached slaughter 12.8 days earlier (P<0.001), had 7.99 kg heavier carcass (P<0.001) and a greater fat score (P<0.001) compared with the progeny of beef cows. Beef cow progeny had a superior conformation score (0.5; P<0.001) and achieved a greater price per kilogram (P<0.001) compared with progeny from BDX. Beef cull cows had a heavier carcass (5.58 kg), superior carcass conformation, greater carcass price per kilogram and greater overall carcass value (P<0.001) than BDX. Results from this study show that replacement strategy is of fundamental importance depending on the type of system implemented by farmers and consideration must be given to the traits of importance within the context of the individual production system.

A proposal of Carcass Grading System for Cull Cows Using Principal Component Analysis

A proposal of Carcass Grading System for Cull Cows Using Principal Component Analysis

Correction: Evaluating the impact of meloxicam oral suspension administered at parturition on subsequent production, health, and culling in dairy cows: A randomized clinical field trial.

[This corrects the article DOI: 10.1371/journal.pone.0209236.].

In vitro subcellular characterization of flunixin liver metabolism in heifers, steers, and cows

The majority of cattle found to have violative liver residues of flunixin (FNX) in the United States are dairy cows. It has been hypothesized that illness of cows decreases the rate of FNX metabolism, resulting in violative residues at slaughter. Another contributing factor might be an age-related decrease in FNX metabolism, as dairy cull cows are typically older at slaughter than cattle raised for beef, rather than milk production. In order to investigate this possibility, subcellular fractions were prepared from liver slices from steers (n = 6) and heifers (n = 5) <30 months of age, and cows (n = 8) >48 mos of age. Cytochrome P450 (P450), NADPH-P450 reductase and glucose-6-phosphate dehydrogenase (G6PDH) activity and rate of 5-hydroxy FNX (5-OH FNX) formation were measured in liver homogenate, cytosolic, microsomal, and S9 fractions. Cows had lower concentrations of P450, NADPH-P450 reductase activity, and 5-OH FNX formation (P ≤ 0. 02), supporting the theory that advanced age may contribute to the higher incidence of violative FNX residues in dairy cows.

Sampling Methodology to Maximize the Efficient Use of National Abattoir Surveillance: Using Archived Sera to Substantiate Freedom From Bluetongue Virus Infection in Ireland.

In recent years, there has been increasing recognition of the value of multiple data sources available to fulfill surveillance objectives, and the use of these has been applied to address many questions relating to animal health surveillance. In Ireland, we face a slightly different problem, namely, best use of an existing surveillance resource (serological samples collected over many years from cull cows at slaughter), which has been used to substantiate freedom from Brucella abortus following its successful eradication in 2009. In this study, we evaluate a sampling methodology to use this resource to substantiate freedom from bluetongue virus (BTV) infection. An examination of the degree to which cull cows were resident in the same herd throughout the midge biting season showed that, of 50,640 samples collected between 17 October and 23 December 2016, 80.2% were from animals resident in the same herd between 01 April 2016 and 2 months prior to their slaughter date, 74.1% for 1 month prior, 70.1% for 2 weeks prior, 66.4% for 1 week prior, and 56.4% up to 1 day prior to slaughter. An examination was made of the degree to which individual samples within the same 88-well frozen storage block came from geographically clustered herds, whether from a concentration of animals from the same herd in a single block, or from clustering around the slaughterhouse where the samples were taken. On the basis of these analyses, a sampling strategy was derived aimed at minimizing the number of storage blocks which needed to be thawed, whilst ensuring a large enough and representative sample, geographically stratified according to the bovine population of 51 squares, each 45 × 45 km, covering the entirety of Ireland. None of the 503 samples tested were positive for BTV, providing reassurance of national BTV freedom. More broadly, the study demonstrates the use of abattoir-based serological samples collected for one large scale surveillance programme in surveillance for other bovine infections.

Effect of sex and sub-zero storage temperature on the microbial and oxidative stability of beef packed in a high-oxygen atmosphere after different vacuum ageing times

This study aimed to evaluate the effect of sex and sub-zero storage temperature on the microbial and oxidative stability of Belgian Blue beef packed in a high-oxygen atmosphere after different ageing times. Longissimus thoracis et lumborum from Belgian Blue young bulls and cull cows were aged at −1 or 4 °C for 80 days in vacuum. Every 20 days, samples were repackaged in a high-oxygen atmosphere (70/30% O2/CO2) and stored for 7 days (2 days at 4 °C + 5 days at 8 °C). Ageing at −1 °C had a protective effect against the growth of lactic acid bacteria and Enterobacteriaceae and myoglobin oxidation. Brochothrix thermosphacta was the limiting parameter for ageing longer than 20 days at −1 °C, permitting a subsequent 7-day shelf-life in a high-oxygen atmosphere. Meat from young bulls was more sensitive to oxidation than meat from cull cows. Extending Belgian Blue meat ageing for >20 days had a negative impact on retail shelf-life.