Cover Crop Mixtures Research Articles

Cover crops improve soil structure and change organic carbon distribution in macroaggregate fractions

Abstract. Soil structure is sensitive to intensive soil management. It can be ameliorated by a reduction in soil cultivation and stimulation of plant and microbial mediators for aggregate formation, with the latter being a prerequisite and measure for soil quality. Cover crops (CCs) are part of an integrated approach to stabilize or improve soil quality. Thereby, the incorporation of diverse CC mixtures is hypothesized to increase the positive effects of CC applications. This study entailed an investigation of the legacy effect of CCs on soil aggregates after three crop rotations in the second main crop (winter wheat) after the last CC treatment. Four CCs (mustard, phacelia, clover, and oat) cultivated in pure stands and with a fallow treatment were compared to a mixture of the four CC species (Mix4) and a highly diverse 12-plant-species mixture (Mix12) in a long-term field experiment in Germany. The organic carbon (OC) distribution within macroaggregate fractions (16–8, 8–4, 4–2, 2–1, and <1 mm) and their aggregate stability were measured by dry- and wet-sieving methods, and the mean weight diameter (MWD) was calculated from water-stable aggregates. The results showed that, compared to the fallow, all CCs increased the MWD between 10 % and 19 % in soil under the following main crop. The average MWD increase over the fallow was slightly higher for CC mixtures (16 %) than for single CCs (12 %). Most of the OC (67.9 % on average) was stored in the <1 mm aggregate fraction, highest in the topsoil and decreasing with soil depth. The intermediate fractions (8–4 mm, 4–2 mm, 2–1 mm) stored 8.5 %, 10.5 %, and 11.0 % of the total OC, while 2.1 % was stored in the 16–8 mm fraction. Higher MWD improvement at the 20–30 cm depth also indicates additional benefits from a reduction in the cultivation depth. Structural equation modelling (SEM) suggests that single CCs were more likely to increase OC storage in small macroaggregates <1 mm, while CC mixtures were more likely to increase OC in the largest fraction (8–16 mm). Different individual CC species or mixtures exhibited varying involvement in the formation of different aggregate fractions. We provide evidence that litter quality, root morphology, and rhizosphere input, which affect microbial mediators of aggregate formation, might be the main reasons for the observed differences between CC treatments. Cover crops are valuable multifunctional tools for sustainable soil management. Here, we showed that they contribute to structure amelioration in arable soils. Increasing the functional diversity of plant species in CC mixtures could be a strategy to further enhance the positive effects of CCs in agroecosystems.

Productivity benefits of cereal-legume cover crop mixtures under variable soil nitrogen and termination times

Mixtures of cereal and legume cover crops can merge the benefits of these two functional groups and sometimes increase biomass production and ecosystem services compared to monocultures. However, the multifunctionality and productivity of cover crop mixtures depend on environmental and management factors that influence species composition. We hypothesized that (i) the species composition in the cereal/legume cover crop mixtures will change in response to soil N level and growth stage, (ii) cereal/legume mixtures will accumulate more aboveground biomass and N and produce greater leaf area index and canopy cover than monocultures, particularly at low soil N levels and with late termination, and (iii) cereal/legume mixtures will reduce the risk of high C:N ratios associated with late termination of a cereal cover crop. We evaluated cereal/legume cover crop mixtures and monocultures in a long-term maize (Zea mays L.) N rate study at the University of Kentucky. The study had four inorganic N rates applied to maize since 1970 (0, 84, 168, 336 kg N ha-1) and three winter cover crop treatments (cereal rye (Secale cereale L.) monoculture, crimson clover (Trifolium incarnatum L.) monoculture, rye/clover mixture). We evaluated the cover crops from fall 2020 through spring 2022. We collected repeated measurements of cover crop aboveground biomass, N content, leaf area index, and canopy cover. At the final sampling in late April or early May, we found that the cover crop mixture produced more biomass than both monocultures at intermediate N input rates, while low and high N input rates resulted in similar biomass levels as monoculture clover and rye, respectively. The treatment without N fertilizer application produced proportionally more clover in the mixture than in higher fertilizer rates. Clover in monoculture did not show significant differences in biomass among the N fertilizer rates, while rye increased its growth proportionally to the applied N. Early termination represented more biomass for rye in mixture, while late termination favored clover growth. The cereal/legume mixtures had moderate C:N ratios relative to both monocultures across N rates and sampling dates. The results of this study suggest that cover crop mixtures may perform equally or better than monoculture across different N levels and termination timings.

Mixture of winter cover crops reduces surface runoff and sediment production under no‐tillage system for Oxisols

AbstractOne of Brazil's efficient soil and water conservation practices is the no‐tillage system (NTS). Nonetheless, erosion during rainfall still causes soil and water losses in this system. Therefore, this study aimed to assess surface runoff in different cultivation systems and sediment production during rainfall events. In macro‐plot 1 (non‐terraced catchment [NTC]), we adopted the NTS management with up‐ and down‐slope farming without including mechanical runoff control practices, which most regional producers use. In macro‐plot 2 (best management practices [BMPs]), we optimized crop rotation by incorporating a mixture of cover crop species in autumn and implemented contour farming practices. In macroplot 3 (terraced catchment [TC]), we adopted the same soil management practices as the NTC macroplot, including mechanical runoff control using broad‐based terraces. We used rainfall data to create hyetographs, hydrographs, and sedimentographs, aiming to evaluate the impact of management practices on surface runoff and sediment production. The surface runoff was reduced by 81% (BMPs) and 88% (TC) compared to the NTC system. There was also a reduction in suspended sediment concentration, around 33% (BMPs) and 63% (TC), compared to the NTC system. Despite conservation systems have shown effectiveness in reducing surface runoff and sediment production, monitoring these systems during periods not influenced by the La Niña phenomenon is necessary to assess the impact of rainfall events on soils with conservation practices in extreme events. The study findings provide guidance and recommendations for agricultural producers and field technicians globally, offering criteria for selecting optimal soil and water management practices in no‐till systems. This promotes a conservation‐oriented approach to agriculture.

Effect of cover crops mixtures on weed suppression capacity in a dry sub-humid environment of Argentina

Cover crops (CC) are increasingly used worldwide within integrated weed management scenarios. They are usually established between two commercial crops and are not harvested, grazed, or incorporated into the soil, but remain on the surface during their growth cycle. The aim of this work was to determine the performance of different CC mixtures and their effects on weed suppression in the south-central region of Buenos Aires province, Argentina. Field experiments were carried out in 2019 and 2020 at the CEI Barrow (MDA-INTA, Tres Arroyos), where both binary and ternary CC mixtures were evaluated. Binary mixtures consisted of winter cereals (Avena sativa, Secale cereale) and legumes (Vicia villosa, Vicia sativa) while ternary mixtures were obtained by combining binary mixtures with canola (Brassica napus). Weed emergence counting was performed on a 14- day basis to determine weed total density. Vegetation cover of the CC mixtures as well as biomass production from crops and weeds were estimated. The specific composition of the mixtures showed a greater influence on vegetation cover than on biomass production, which would depend mainly on the prevailing environmental conditions. The type of vetch used, the addition of canola, as well as, the proportion of cereals in the mixture determined the CC effect on weeds. CC were consistently more effective in suppressing weed biomass than seedling emergence density. However, weed seedling suppression by CC was similar to or even greater than the chemical-based control (p<0.0001). The average biomass of weeds in all CC (pooled data) was highly reduced when compared to the weedy control (12 vs 259 g m-2, p<0.001), and similar responses were obtained when compared to the chemical fallow (8 g m-2). The mixture S. cereal+ V. villosa+ canola stood out for presenting the highest values of productivity and vegetation cover and high weed suppression. These results provide support for the choice of CC mixtures in a dry sub-humid area of Argentina, aimed at maximizing interference with weeds and also to demonstrate their benefits in short- and long-term management. Therefore, CC implementation within crop sequences should be considered as a complementary tool contributing to the development of more sustainable management strategies.

Combined effect of cover crops and bio-fertilizer on sustainable popcorn maize production.

Cover crops play an important role in low-input cropping systems, increasing the use of agro-ecosystem services. Due to the lack of information about the impact of cover crops and bio-fertilizers on popcorn maize (Zea mays everta Sturt.) growth and yield quality, especially the popping volume and nutritive quality, such as concentrations of protein and mineral elements, this research aimed to provide essential information. The interrelation between popcorn maize productivity and quality with important groups of soil microorganisms presents additional novelty. The results demonstrated that field pea is a beneficial cover crop, especially when combined with a bio-fertilizer, supporting the accumulation of maize biomass, chlorophyll, yield potential, and the concentrations of protein, Ca, Mg, Fe, and Zn. In addition, field pea residues promoted N-fixing bacteria, and the number of total microorganisms, especially actinomycetes and decomposing bacteria, which could promote nutrient uptake and grain quality. Residues of cover crop mixtures, common vetch + winter oats and field pea + winter oats, promoted the total number of microorganisms in the soil, and up to the end of vegetation, a greater number of decomposition and ammonification microorganisms were found, especially when the bio-fertilizer was applied, which consequently could support greater maize biomass. Popping volume, as a main trait of popcorn maize, had the highest value in the common vetch + winter oats variant, supporting again the statement that quality traits could be enhanced in sustainable production. Unlike living cover crops, mulch mainly affected soil microbial communities and promoted the development of actinomycetes and cellulolytic microorganisms during the growing season. The results of this research could contribute to the development of sustainable popcorn maize production for improved grain quality. They could also serve as a basis for isolating beneficial soil microorganisms to develop new bio-fertilizers that could improve maize production in synergy with cover crops.

Effects of Different Cover Crops and Amendments on Soil and Crop Properties in Organic Vegetable Production

The impacts of two different cover crop (CC) strategies, as well as compost, silicon (Si), and biocontrol (BC) soil amendments were evaluated on soil chemical and biological properties, crop development and yield, and disease and pest issues in organic vegetable production, as represented by legume (green snap bean), cucurbit (green zucchini squash), and solanaceous (sweet pepper) vegetable crops, in a three-year field trial in Maine, USA. A multi-species CC mixture (6 crops, including legumes, grasses, and brassicas) was compared with a standard winter rye CC for potential benefits on soil properties and biomass production. Soil amendments included a commercial organic fertilizer alone, composted dairy manure, compost plus BC (commercial formulations of Trichoderma and Streptomyces spp.), and compost plus Wollastonite, a natural source of Si. Poor stand establishment of some components of the multi-species CC mixture resulted in lower biomass and ground cover relative to winter rye, but had no effect on crop development or yield. Compost amendments increased soil pH, organic matter, and nutrient contents, as well as yields of bean, zucchini, and peppers relative to a fertilizer-only treatment. Additions of Si increased Si content in plant leaves and reduced powdery mildew on squash and leaf necrosis of beans. In the absence of substantial soilborne disease, BC provided only marginal reductions in powdery mildew and leaf necrosis and no effects on yield. These results help define specific management practices to improve organic vegetable production and provide useful information and options for growers.

Cover crop diversity for sustainable agriculture: Insights from the Cerrado biome

AbstractBrazil is one of the largest soybean producer of the world and the Cerrado biome has played a pivotal role in this expansion. Because of the economic and agronomic challenges associated with the maize production as a second summer crop in this region, cover crops are gaining popularity as a strategy to diversify the agricultural system while enhancing soil health. This study assessed the benefits of single species of cover crops and a mix of cover crop species in between harvest seasons to the soybean grain yield and nematode suppression. The study was carried out for 3 years in two locations within the Cerrado biome. We evaluated six cover crop treatments after soybean cultivation: (1) Mix of cover crops (Pennisetum glaucum, Crotalaria spectabilis and Urochloa ruziziensis), (2) P. glaucum (Pearl millet), (3) C. spectabilis, (4) U. ruziziensis (Congo grass) (5) Urochloa brizantha cv. Marandu (Marandu palisadegrass) and (6) U. brizantha BRS Paiaguás (Paiaguás palisadegrass). Pennisetum glaucum and U. brizantha cv. Marandu produced highest amounts of biomass on a 3‐year average. In one site, P. glaucum produced more biomass than other cover crops by 210%. Tropical grasses (U. ruziziensis, Marandu and Paiaguás), along with the cover crop mixture, exhibited intermediate biomass levels in the site with higher P. glaucum biomass production and did not differ from P. glaucum in the other site. Cover crops varied nutrient uptake depending on the species. Decomposition rates varied among cover crops as expected, with C. spectabilis decomposing rapidly and releasing substantial amounts nutrients, particularly nitrogen. In contrast, the cover crop mixture had a slower decomposition. The choice of cover crop significantly influenced soybean population and yield, with some variability across years and locations. The cover crop mixture consistently supported higher soybean populations and yields, highlighting its potential for enhancing soybean production, nutrient cycling and nematode suppression. It effectively reduced nematode abundance in soybean roots, highlighting its role in nematode management. Our findings emphasize the robustness and versatility of cover crop mixtures in mitigating weather variability across years and sites. They consistently performed well in terms of biomass production, nutrient uptake, soybean yields and nematode control. This study highlights the vital role of cover crops in the Cerrado ecosystem, enhancing soil health, crop productivity and environmental sustainability. The choice of cover crop species and mixtures offers a valuable tool for farmers seeking resilient and sustainable agricultural practices amid changing environmental conditions.

Cover crop effects on the growth of perennial weeds in two long-term organic crop rotations

Abstract Cover crops are important in organic arable cropping systems because they improve soil fertility and suppress annual weeds in the post-harvest period (the period between cash crops), but their effectiveness against perennial weeds is less clear. This study analyzed outbreaks of perennial weeds in two long-term organic crop rotations with and without cover crops in the period 2011–2022 in Denmark. In addition, the impact of including forage plantain (Plantago lanceolata) in cover crop mixtures on the growth of perennial weeds was studied from 2020 to 2022. P. lanceolata is known to contain secondary metabolites that inhibit nitrification and may influence nitrogen (N) availability. This study found that cover crops did not hinder outbreaks of Cirsium arvense, Sonchus arvensis, Elytrigia repens or other perennial weed species. E. repens actually proliferated more with cover crops whose presence prevented repeated mechanical post-harvest interventions. The crop rotation involving regular cutting of a one-year or two-year green manure crop provided the best management of perennial weeds if repeated post-harvest soil cultivation was also conducted. This could only be done in the absence of cover crops. Mixing P. lanceolata into the cover crop mixtures did not change the outcome of competition between cover crops and perennial weeds in the period between crop harvest in August and November. Instead, P. lanceolata significantly reduced the number of shoots emerging from perennial weeds in the subsequent growing season. The causality of this effect was not further elaborated in the study.

Integrating cover crops and herbicides for weed control in soybean

Abstract In mid-southern, southeastern, and northeastern U.S. soybean production regions, the evolution of herbicide-resistant weeds has become a significant management challenge for growers. The rising herbicide costs for managing herbicide-resistant weeds are also a growing concern, leading to the utilization of cover crops as an integrated weed management strategy for addressing these challenges. Field experiments were conducted at two locations in Alabama in 2022 to evaluate winter cereal cover crops, including a mixture and herbicide system integration in soybean. Treatments included five cover crops: oats, cereal rye, crimson clover, radish, and a cover crop mixture. Cover crops were evaluated for their weed-suppressive characteristics compared to a winter fallow treatment. Additionally, four herbicide treatments were applied: a preemergence (PRE) herbicide, a postemergence (POST) herbicide, PRE plus POST herbicides, and a nontreated (NT) check. The PRE herbicide was S-metolachlor; the POST treatment contained a mixture of dicamba and glyphosate. The PRE plus POST system contained the PRE application followed by POST application. Results show that cereal rye and the cover crop mixture provided weed biomass reduction compared to all cover crop treatments across both locations. Furthermore, we observed greater soybean yield following the cereal rye cover crop than following the winter fallow treatment at one location. POST and PRE plus POST herbicide treatment resulted in greater weed biomass reduction and improved soybean yield than the PRE herbicide treatment alone and the NT check at both locations.

Optimizing ecosystem function multifunctionality with cover crops for improved agronomic and environmental outcomes in dryland cropping systems

CONTEXTIntegrating cover crop mixtures into dryland crop-fallow rotations may foster ecological intensification by enhancing ecosystem function multifunctionality compared with conventional fallows and monoculture cover crops. However, the expression of functional traits in cover crop mixtures is influenced by arrays of agronomic and environmental filters that differently influence cover crop performance. OBJECTIVETo (1) determine the identity and diversity effects of different cover crop functional types on ecosystem functions and trade-offs, and (2) determine the optimal cover crop mixture that maximizes ecosystem function multifunctionality in a subtropical dryland environment. METHODSUsing three-site-year field experiments, we assessed if cover crop mixtures enhanced ecosystem function multifunctionality (MF) above that of monoculture cover crops and conventional fallow. We measured a range of ecosystem functions as indicators of three major ecosystem service categories: 1) provisioning services (biomass production, cash crop yield, and profitability); 2) supporting services (weed suppression, soil active carbon accrual, soil aggregate distribution, nitrogen (N) mineralization, N supply, and N retention); and 3) regulating services (parasitic nematode suppression, promotion of free-living nematodes, soil respiration, and soil water conservation). RESULTS AND CONCLUSIONSWe found greater suite of ecosystem functions following mixture cover crops compared to monocultures, likely due to synergistic trait combinations that increased ecosystem function multifunctionality. The legume and forage brassica monocultures had consistently low multifunctionality (MF < 0.5). The oat:legume mixture resulted in the highest multifunctionality (MF > 0.5), driven by moderate water use and aboveground biomass production, and high groundcover residency, thus favouring higher cash crop yields and system profitability. They also generated overall improvements in soil health by providing additional carbon inputs, increasing the proportion of larger soil aggregates, and improving soil food web structure. We predicted the optimum cover crop mixture to maximize ecosystem function multifunctionality was 64% oat, 36% legume and 0% forage brassica. SIGNIFICANCEThis study offers novel insight into the agronomic and environmental impacts of replacing fallow with cover crops in a subtropical dryland. It shows that integrating mixture of oat:legume cover crops can increase multifunctionality. Further research is required on the extent of ecosystem trade-offs between competing ecosystem functions. The predicted optimum cover crop mixture composition will likely vary when different ecosystem functions are considered or if long-term experiment data are used. Hence, the recommended optimum cover crop mixture must be interpreted with caution and cover crop selection should be based on practical recommendations according to target management goals.

Cover crop-mediated soil carbon storage and soil health in semi-arid irrigated cropping systems

Cover cropping is considered a climate-smart strategy to regenerate low-fertility soils in arid and semi-arid regions. However, the mechanisms of soil organic carbon (SOC) storage and soil health improvement in semi-arid irrigated cropping systems are poorly understood. The main goal of this study was to evaluate cover crop effects on various physical, chemical, and biological indicators of soil health in an irrigated silage corn (Zea mays L.)–sorghum [Sorghum bicolor (L.) Moench] rotation and understanding how changes in soil health contribute to SOC storage. We evaluated various SOC and nitrogen (N) pools, and microbial community structure under cover crop mixture of grasses, brassicas, and legumes (GBL), grasses and brassicas (GB), grasses and legumes (GL), and no cover crops (NCC) control for four years. Grasses included annual ryegrass (Lolium multiflorum Lam.) and winter triticale (× Triticosecale Wittmack), brassicas included turnip (Brassica rapa subsp. rapa L.) and daikon radish (Raphanus sativus var. Longipinnatus Bailey), and legumes included pea (Pisum sativum subsp. arvense L.) and berseem clover (Trifolium alexandrinum L.). Soil C pools: SOC, potentially mineralizable C, microbial biomass C, mineral-associated organic C (MAOC), and particulate organic C (POC) were greater with cover crops than without, but the effects were not consistent in all study years. Soil organic N was also greater under cover crops than under NCC. Soil C-to-N (C:N) ratio increased over the years but was similar among treatments. Cover crops used N and soil water to grow, significantly reducing soil inorganic N content and water-filled pore spaces in the first two years. However, fungal and bacterial communities grew better under cover crops with a high fungi-to-bacteria ratio than under NCC. The soil MAOC content was related to microbial community and enzyme activity while POC was affected by soil physicochemical conditions, specifically soil pH and mineral N availability, highlighting two major pathways of SOC sequestration in semi-arid cropping systems. This study showed how cover crops enhance SOC storage and soil health in semi-arid cropping systems.

Cover crop functional types alter soil nematode community composition and structure in dryland crop-fallow rotations

Increasing dryland cropping intensity by integrating cover crops into crop-fallow rotations can add diversity and alter soil ecosystem functions, including improvements in soil nutrient and water retention and cycling, and suppression of soil-borne pests. However, the use of cover crops can also carry the risk of increasing the abundance of plant-parasitic nematodes that could undermine system productivity. Here, we examined how the integration of diverse cover crops of different functional groups: Poaceae [forage oat (Avena sativa L.)]; Brassicaceae [forage rape (Brassica napus L.)]; and Fabaceae [common vetch (Vicia sativa subsp. sativa L. and fababean (Vicia faba L.) in monocultures and mixtures into a dryland crop-fallow rotation altered soil nematode community composition and structure relative to conventional fallows. We found that cover crop mixtures had greater diversity and complexity of free-living nematodes, indicating enhanced cycling of energy and nutrients (enrichment index >50 %) compared to monocultures. Furthermore, using a structural equation modelling approach, we showed the abundance of free-living nematodes is driven primarily by cover crop residue quality (C:N ratio, and cellulose content) and marginally by cover crop quantity (biomass). The grass-legume mixture had the highest abundance of plant-parasitic nematodes (Pratylenchus neglectus and Merlinius brevidens) but also fostered robust soil food webs with complex soil nematode trophic interactions, indicating promotion of soil ecosystem functions related to N and C cycling and overall improvement in soil health. The results indicate bottom-up effects of cover crops on soil nematode communities are modulated by cover crop functional type and mixture composition. Consequently, cover crops with appropriate traits must be selected if soil health is to be improved via suppression of plant-parasitic nematodes, promotion of free-living nematodes, and enhancement of soil food web complexity.

Impact of different establishment methods for summer-sown cover crops in Southwestern Germany on their weed suppression properties

Early establishment of cover crops (CCs) is essential for attaining successful weed suppression in the autumn-to-spring period. Due to reduced precipitation and extended periods of drought in the summer within temperate zones, the establishment of CCs poses challenges. Three methods of cover crop (CC) establishment were tested: 1) preharvest sowing 10 days before harvest of cash crop (PreHa); 2) no-till sowing directly after harvest of cash crop (NoTi) and 3) sowing with reduced tillage 10 days after harvest of cash crop (ReTi). The five CC species black oat (Avena strigosa Schreb.); common buckwheat (Fagopyrum esculentum Moench), phacelia (Phacelia tanacetifolia Benth.), fodder radish (Raphanus sativus var. oleiformis L.) and common vetch (Vicia sativa L.), along with a mixture comprising 20% of all five species, were included as second factor in a three-year field experiment (2020–2022) conducted in South West Germany. One treatment per establishment method was maintained without CCs as a control. Measurements of CC, volunteer cereal and weed density, soil coverage, and dry matter were carried out throughout the autumn-to-late autumn timeframe. On average across all years and CCs, the PreHa and NoTi establishment demonstrated the highest CC dry matter contents of 99 g m−2 and 119 g m−2, respectively, despite lower CC densities. This had a particularly notable impact on weed density in the ReTi variant, which, over the three-year average across all CC treatments, was 5 times higher than in NoTi and PreHa but exhibited a 18–27% smaller volunteer cereal density by comparison, thereby influencing the respective volunteer cereal and weed coverages. Meanwhile, weed and volunteer cereal dry matters were consistent across all establishment methods. The CC mixture exhibited enhanced resilience to fluctuating weather conditions, leading to an overall superior performance across the three-year experimental period and showing highest measured CC dry matters up to 272 g m−2 in 2022. However, the observed effects in the treatments varied considerably depending on the CC species, sowing date, and weather conditions. In summary, this study demonstrates that early CC sowing, whether directly after the cash crop harvest or 10 days before the cash crop is harvested, has a positive impact on CC biomass formation, especially leading to a reduced weed emergence. Additionally, under variable weather conditions, a diverse CC mixture can exhibit a more stable overall performance compared to CC monocultures.

Cover cropping promotes soil carbon sequestration by enhancing microaggregate-protected and mineral-associated carbon

Cover cropping can improve soil C sequestration compared to no cover cropping, but the mechanism of C sequestration in soil aggregates and minerals needs more exploration. We explored C sequestration using C fractions in soil aggregates and minerals by cover crops in a five-year old summer cover crop – winter wheat (Triticum aestivum L.) rotation system at Changwu National Agroecology Experimental Station in the Chinese Loess Plateau. Three cover crops as soybean (Glycine max L., SB), sudangrass (Sorghum sudanense (Piper) Stapf, SG), soybean and sudangrass mixture (SS) were planted during summer fallow and incorporated into the soil two weeks prior to wheat planting each year. Soil samples at 0–10, 10–20, and 20–40 cm depths were collected at wheat harvest after 5-yr and analyzed for C fractions which were coarse particulate organic C (cPOC), intra-microaggregate fine particulate organic C (iPOC), free fine particulate organic C (fPOC), and mineral-associated organic C (MOC). The iPOC and MOC are considered as protected C against mineralization. Compared to no cover crop (CK), cover crops increased large macroaggregate proportion at 0–10 cm by 18–22 %, with SS having a greater mean-weight diameter (MWD) than other treatments. Cover crops had greater MOC, iPOC, and fPOC fractions than CK in most aggregate-size classes and the bulk soil at all depths, with SS having the greatest C fractions. The MOC was greater than any other C fractions, regardless of cover crop species. Cover cropping can enhance soil aggregation and C sequestration by increasing microaggregate-protected and mineral-associated C compared to no cover cropping, resulting in improved soil C stabilization. Cover crop mixture was more effective in promoting soil aggregate stability and C fractions than single cover crop species.

PSI-A-12 Evaluation of Novel and Traditional Cover Crops Utilizing Cattle in High-Density, Short-Duration Grazing

Abstract The objective was to evaluate yield, nutritive value, and neutral detergent fiber digestibility (NDFD) of traditional and non-traditional cover crops utilizing short-duration, high-density grazing. This experiment was conducted at Western Illinois University Kerr Farm, Macomb, Il. Cow-calf pairs (n = 58) grazed 0.4046 hectare plots in spring 2021. Dry cows (n = 58) grazed 0.4046 hectare plots in the fall 2021. Grazing duration was 24 hours per plot. In the spring, five plots of rye were grazed. In the fall, two plots of kernza, two plots of cover crop mix (CMIX), and one plot of red clover were grazed. The cover crop mix contained 12 varieties including brassicas, legumes, cereal grains, and grasses. Kernza is a novel perennial crop harvested for grain in the summer and used for grazing in the fall and winter. Plots were randomly sampled four times pre and post grazing using a 0.4047 square meter forage ring. All grass within the ring was clipped leaving 10.16-15.24 cm of material to mimic grazing. Forage height was recorded using a tape measure. Samples were collected and dried prior to combining and averaging data. Data pre- and post-grazing included DM, forage height, and DM yield. Cattle intake was calculated using pre- and post-grazing DM yields. Samples were analyzed at Rock River Laboratories (Watertown, WI) for CP, aNDF, ADF, and NDFD at 12, 30, and 48 hours. Data were analyzed using the Mixed procedure of SAS 9.4. Means were determined using LSMeans and separated using the p-diff function. Pre-grazing height of rye was 141 cm, CMIX was 87.6 cm, while kernza and red clover were both 26.7 cm (P &amp;lt; 0.01). Rye was most abundant in kg/ha of DM pre-graze and post-graze (P &amp;lt; 0.01). Cows consumed over 50% of kernza and CMIX, but only 29% of rye (P &amp;lt; 0.01). Forage dry matter percentages did not differ. Kernza had a lower CP (6.36%) than rye (8.91%; P = 0.02). Kernza CP did not differ from CMIX at 7.78% (P = 0.19). Kernza had a 7.18% greater aNDF than rye (P = 0.04). The aNDF of CMIX (55.56%) was greater compared with red clover at 44.75% (P = 0.04). Kernza ADF was 49.22% compared with rye at 43.8% (P = 0.02). The CMIX had the most desirable NDFD at 12, 24, and 48 hours (P &amp;lt; 0.01). Data indicated that rye, kernza, red clover, and cover crop mixtures offer value in high-intensity, short-duration cover crop grazing in terms of nutritive value and NDFD for cattle. Rye had the greatest DM yield values but less consumption by cattle due to forage maturity.

Impact of Cover Cropping on Temporal Nutrient Distribution and Availability in the Soil

Cover cropping is a best management practice that can improve soil quality by reducing soil erosion, building soil organic matter (SOM), and improving soil nutrient availability. Southwest (SW) Florida citrus growers have the challenge of growing citrus in sandy soils characterized by low organic matter (&lt;2%), extremely low water and nutrient-holding capacities, and high sand content (&gt;90%), and therefore are looking for methods to improve SOM and nutrient retention and availability in sandy soils. A trial of two cover crop (CC) mixtures planted in the row middles (RM) of Huanglongbing-affected citrus ‘Valencia’ (Citrus sinensis (L.) Osbeck) orchards in sandy soils in SW Florida was conducted. This study explored how incorporating CCs in the RM of the orchards could affect soil ammonium (NH4+), soil nitrate (NO3−), exchangeable macronutrients, and SOM temporal availability. These parameters were measured under the tree canopy (UC) and within RM of two orchards: South Grove (SG) and North Grove (NG), both located in SW Florida. The two seeded CC mixtures were legume + non-legume (LG+NL) and non-legume (NL) and were compared to a control no-CC grower standard (GSC). Phosphorus, calcium, magnesium, and NH4+ were not statistically significantly different among treatments in either of the two sampling positions (UC and RM). Cover cropping significantly (p &lt; 0.05) increased NO3−-N concentrations in the RM area of the citrus orchards after seven consecutive seasons (brassicas, legumes, and grasses) by 31% in the LG + NL and 29% in the NL with reference to the GSC. In addition to the significant increase in NO3−N, SOM significantly (p &lt; 0.05) increased in the RM in the NG site only in both CCs treatments by 17% and 16% for LG + NL and NL treatments, respectively, compared with GSC.

Análise multivariada como ferramenta na seleção de agroecossistemas sustentáveis de cultivo de meloeiro no Semiárido

ABSTRACT Soil management systems, including tillage and green manuring, are important for the control of nematodes and for the sustainable production. The objective was to select, through multivariate analysis, sustainable agroecosystems for yellow melon cultivated under irrigated conditions in the Brazilian semi-arid region. The long-term experiment is set up in Argissolo Vermelho- Amarelo (Ultisol) with six multifunctional agroecosystems, composed of the combination of three mixtures of cover crops (1 - 75% legumes + 25% grasses and oilseeds; 2 - 25% legumes + 75% grasses and oilseeds and 3 - spontaneous vegetation) and two types of soil management (no tillage-NT and tillage-T). The purpose was to evaluate the nutritional status of plants, soil fertility, nematode population in roots and soil, and the yield. Data were analyzed with multivariate techniques, allowing the grouping of the agroecosystems based on their similarities and complexity of functions. The incorporation of plant residues to the soil reduces nematode population, but their maintenance on the surface allows the improvement of fertility and yield, even under larger nematode populations. The agroecosystems that associate the nonincorporation of residues from cover crops with larger species diversity in composition constitute the most complex model, allowing different environmental functionalities. Thus, it is recommended not to incorporate the plant residues, planting the melon seedlings directly in the straw. However, considering that the results obtained with the non-incorporation of spontaneous vegetation were similar to those obtained with the incorporation of commercial cover crops, it is concluded that this practice is also viable and adapted to the semi-arid conditions.

Oil radish, winter rye and crimson clover: root and shoot performance in cover crop mixtures

Abstract Purpose The current knowledge of belowground interactions in intercropping systems is limited due to methodological constraints. The current study aimed to investigate cereal-brassica and cereal-legume-brassica cover crop mixtures regarding mixture effects on root and shoot biomass as well as root traits, vertical root niche differentiation, and complementarity. Methods Sole crops and two- and three-species-mixtures of winter rye (Secale cereale L.), crimson clover (Trifolium incarnatum L.), and oil radish (Raphanus sativus L. var. oleiformis Pers.) were grown in Germany in a two-year organic field experiment. Root traits were analysed using the monolith method. For discrimination of species root mass Fourier transform infrared (FTIR) spectroscopy was used. Results Oil radish dominated mixtures above- and belowground. Oil radish and its mixtures had highest root length density (RLD) and root mass density (RMD) in subsoil. Rye had highest root biomass and RLD in topsoil. Clover was uncompetitive and had low RLD and RMD. Large but non-significant mixture effects occurred in the shoot, especially for shoot nutrient uptake. Mixture effects were positive for RLD and RMD in subsoil and positive for specific root length (SRL) throughout the whole profile. There was no clear evidence for vertical root niche differentiation and root mass complementarity. Conclusion Oil radish as a mixing partner increased rooting in subsoil. When comparing mixtures and sole crops, morphological changes, i.e. higher SRL in mixtures, were found. Contrary to expectations, changes in root allocation patterns, such as vertical niche differentiation or complementarity of root mass, were not observed.

Effects of paraquat application at cover crop planting on cover crop biomass and weed suppression

AbstractSuccessful cover crop (CC) establishment in the fall is important to maximize CC production, which is critical for achieving many objectives of CCs. Competition from winter weeds may reduce CC establishment and biomass production. A preplant herbicide, such as paraquat, at the time of CC planting in the fall will reduce winter weed pressure resulting in better establishment and growth. An experiment was conducted between 2019 and 2021 to test this hypothesis by evaluating a no-CC check, cereal rye, hairy vetch, crimson clover, and cereal rye + hairy vetch drilled with and without paraquat applied at planting (mid-October to mid-November) following either a corn or soybean crop. Visible weed suppression ratings were collected in mid-April, and total CC and weed biomass was collected in late April. More CC biomass was accumulated following corn than soybean, regardless of preplant herbicide application, because corn is typically harvested before soybeans. Therefore, CCs should be planted early to accumulate more biomass. Weed suppression varied by weed species from all factors, but in general weed suppression was best from a CC mixture containing cereal rye and paraquat applied at planting. If weed suppression is the main goal of the CC, then a preplant herbicide at CC planting is recommended. However, if CC weed suppression goals can be achieved through biomass accumulation, no preplant herbicide is needed. This information is useful for producers to achieve various CC objectives while managing costs.

Mixture of winter cover crops improves soil physical properties under no-tillage system in a subtropical environment

The monocrop of winter cover crops is a common practice under no-tillage system (NTS) in subtropical environment but increase diversity of cover crop species can be an alternative to improve soil physical quality, with positive effects on crop production. This study evaluated whether using a greater diversity of cover crop species affects soil physical properties, as well as grain yield of soybean (Glycine max L.) and common bean (Phaseolus vulgaris L.) under NTS in three growing seasons. The experimental design was in randomized blocks with four replications. The treatments were monocrop of black oat (Avena strigosa Schreb.); intercropping of black oat and turnip (Brassica napus L.); and a mixture of black oat, turnip, white oat (Avena sativa L.), rye (Secale cereale L.), white lupine (Lupinus albus L.), and common vetch (Vicia sativa L.). Cover crop biomass yield, soil physical properties, and grain yield of summer crops were evaluated. Using a mixture of cover crops improved soil physical properties by decreasing soil bulk density up to 5 %, increasing soil air-filled porosity at –100 hPa (0.0–0.2 m) up to 27 % and saturated hydraulic conductivity (0.0–0.2 m) up to 300 %. Common bean grain yield and 1000-grain weight in succession to the mixture of cover crops increased by 15 % and 4 %, respectively. Soybean yield components were not affected by treatments. The increased diversity of winter cover crops improved soil physical properties in relation to monocrop in NTS, as well as improved crop yield of common bean, which is a shallower root growth specie, more sensitive to soil constraints.