Courtship Behavior Research Articles

Female contact sex pheromone recognition in the German cockroach (Blattella germanica) is mediated by two male antennae-enriched sensory neuron membrane proteins.

The German cockroach Blattella germanica is a notorious urban health pest that has developed resistance to multiple pesticides. Thus, novel non-lethal pest control agents are urgently needed. Olfaction interference via disruption of sex pheromone recognition-related genes offers a promising approach. The German cockroach has a unique courtship behavior in which female adults emit contact sex pheromones (CSPs) in response to antennal touching, which subsequently triggers distinctive male sex behavioral responses. Due to the limited volatility of CSPs, the molecular mechanisms underlying their recognition and the specific olfactory pathways activated remain poorly defined. Although the odorant receptor coreceptor (Orco) is critical for most insect olfaction, sensory neuron membrane proteins (SNMPs), in particular SNMP1, also play crucial roles in sex pheromone recognition in moths and flies. While multiple SNMP1 homologs have been identified in multiple insect species, they have yet to be fully functionally characterized in cockroaches. In this study, RNA-interference (RNAi)-mediated knockdown of BgerOrco reduced both the electrophysiology responses and courtship behaviors of males, indicating CSP perception proceeds via an olfaction pathway. Similar RNAi knockdown of BgerSNMP1e and BgerSNMP1d, which are predominantly expressed in male antennae, revealed critical roles in perceiving the major component of the Blattella germanica CSP blend. Unlike BgerSNMP1e, BgerSNMP1d was also found to function in the perception of the minor CSP component. Molecular docking analyses revealed no differences in the binding affinities of BgerSNMP1d for the major and minor CSP components, whereas the binding affinities of BgerSNMP1e displayed clear selectivity for the major component. Our results show that the olfactory pathway is critical for CSP recognition and that two male-enriched SNMP genes, BgerSNMP1e and BgerSNMP1d, are crucial factors mediating the male response to CSP stimulation in German cockroaches. This study lays a foundation for studying the mechanisms of CSP recognition and provides novel molecular targets with potential to be exploited as disruptors of courtship behavior. © 2024 Society of Chemical Industry.

Convergent olfactory circuits for courtship in Drosophila revealed by ds -Tango.

Animals exhibit sex-specific behaviors that are governed by sexually dimorphic circuits. One such behavior in male Drosophila melanogaster , courtship, is regulated by various sensory modalities, including olfaction. Here, we reveal how sexually dimorphic olfactory pathways in male flies converge at the third-order, onto lateral horn output neurons, to regulate courtship. To achieve this, we developed ds -Tango, a modified version of the monosynaptic tracing and manipulation tool trans- Tango. In ds -Tango, two distinct configurations of trans- Tango are positioned in series, thus providing selective genetic access not only to the monosynaptic partners of starter neurons but also to their disynaptic connections. Using ds -Tango, we identified a node of convergence for three sexually dimorphic olfactory pathways. Silencing this node results in deficits in sex recognition of potential partners. Our results identify lateral horn output neurons required for proper courtship behavior in male flies and establish ds -Tango as a tool for disynaptic circuit tracing.

Courtship Behavior of Adult Spodoptera frugiperda (Lepidoptera: Noctuidae) Observed Using Track 3D Trajectory Tracking.

Spodoptera frugiperda, also known as the fall armyworm (FAW), is classified by the Food and Agriculture Organization of the United Nations (FAO) as a major agricultural pest. By gaining a more nuanced understanding of the fall armyworm's courtship behavior, simpler and more environmentally friendly methods of controlling this pest can be developed. This study used the Track 3D system to meticulously record and describe the activity characteristics and patterns of adult males and females during courtship. The results show that adult FAWs engaged in a variety of activities during courtship that were either discrete (flying, flapping, moving, and crawling), continuous (flapping + flying, flapping + crawling, and flying + crawling), or combined (flapping + touching + flapping; flying + touching + flying). Flying and flapping were the most common activities, with observed flight patterns consisting of parabolic, circular, and zigzag trajectories. The peak activity times for adult FAWs are mainly concentrated at 11:00 p.m., 3:00 a.m., and 5:00 a.m., providing fundamental data for the precise attraction and control of adult FAWs at later stages.

Don't touch me! Mating not always preceded by courtship behavior in predatory coccinellids.

Knowledge of the ecology and behavior of biological control agents is essential for their effective use in biocontrol and Integrated Pest Management (IPM)programs. There is a lack of information regarding the courtship and mating traits that are crucial for successful mating in biocontrol population of coccinellids. To expand our current understanding in this area, 2 coccinellid species commonly used for the biocontrol of soft-bodied insects, Cryptolaemus montrouzieri (Mulsant) and Propylea quatuordecimpunctata (Linnaeus) (Coleoptera: Coccinellidae), were studied. A quantitative analysis was performed to compare their courtship and mating displays. Key behavioral traits of the precopulatory, copulatory, and postcopulatory phases were investigated. The courtship and mating sequences of C. montrouzieri and P. quatuordecimpunctata were similar to each other. During the mating phase, the key displays were the opening of the elytrae and wings followed by body shaking in C. montrouzieri and leg tapping followed by body shaking in P. quatuordecimpunctata. The mating success of both species was not correlated with any courtship display, such as antennal tapping. Only in C. montrouzieri mounting attempt showed a higher frequency from the backside without affecting male mating success. This research adds baseline knowledge about the courtship and mating behavior of the biocontrol population of coccinellids, contributing to the identification of potentiallyuseful benchmarks (e.g., body shaking, leg tapping) for monitoring prolonged mass-rearing processes, thusreducing mating failures.

Species of Wadicosa (Araneae, Lycosidae): a new species from the southeastern Arabian Peninsula

Wadicosa arabica sp. nov. is described from material of both sexes collected in Oman and the United Arab Emirates. The species belongs to the W. fidelis group and differs from its congroupers, inter alia, by the shape of the tegular apophysis and the embolus in the male. The occurrence of W. arabica sp. nov. is restricted to the gravel, pebble and cobble beds of ephemeral and intermittent streams (wadis) in the mountains of the eastern United Arab Emirates and northern Oman (the Hajar Mountains sensu lato). There it is often common and conspicuous alongside areas of surface waters. Notes on its courtship behaviour are given.

Display court ecology in male Albert’s Lyrebirds

ABSTRACT Display courts are fundamental to the sexual displays of many species of bird; however, the ecology of display courts is poorly understood, representing an important gap in our understanding of the function and evolution of complex animal displays. Here, we describe the biophysical attributes (‘ecology’) of the display courts of male Albert’s Lyrebirds, a ground-dwelling rainforest species that performs a spectacular song and dance display on set courts, known as ‘display platforms’, that are composed of vines or fallen branches. Males rhythmically move these vegetative structures while singing, forming a unique ‘stage shaking’ component of their sexual display. We investigated whether display platforms differed from random locations within a male’s territory in terms of the number of vines or other vegetative structures, area of ground clearing, vegetation density, and canopy cover measurements. We primarily found that platforms had a larger number of vines or other vegetative structures. Platforms also tended to be in areas with more canopy openings. We suggest that platform features amplify the movement of the dancing male, and that male Albert’s Lyrebirds choose platforms in locations that increase their conspicuousness to female receivers.

A modular circuit coordinates the diversification of courtship strategies.

Mate recognition systems evolve rapidly to reinforce the reproductive boundaries between species, but the underlying neural mechanisms remain enigmatic. Here we leveraged the rapid coevolution of female pheromone production and male pheromone perception in Drosophila1,2 to gain insight into how the architecture of mate recognition circuits facilitates their diversification. While in some Drosophila species females produce unique pheromones that act to arouse their conspecific males, the pheromones of most species are sexually monomorphic such that females possess no distinguishing chemosensory signatures that males can use for mate recognition3. We show that Drosophila yakuba males evolved the ability to use a sexually monomorphic pheromone, 7-tricosene, as an excitatory cue to promote courtship. By comparing key nodes in the pheromone circuits across multiple Drosophila species, we reveal that this sensory innovation arises from coordinated peripheral and central circuit adaptations: a distinct subpopulation of sensory neurons has acquired sensitivity to 7-tricosene and, in turn, selectively signals to a distinct subset of P1 neurons in the central brain to trigger courtship. Such a modular circuit organization, in which different sensory inputs can independently couple to parallel courtship control nodes, may facilitate the evolution of mate recognition systems by allowing novel sensory modalities to become linked to male arousal. Together, our findings suggest how peripheral and central circuit adaptations can be flexibly coordinated to underlie the rapid evolution of mate recognition strategies across species.

How Bar Chart Display Features can Skew Perception

This study empirically examines three display design features that can hinder or promote viewers’ perceptual bias of bar charts. Bar charts are commonly used in various landscapes, including business contexts, yet, research on their impact on bias is limited. In an experiment, 266 undergraduates viewed vertical bar charts with two styles of scaling (fine vs coarse), two formats of bar ordering (in a certain order vs in random order) and two patterns of bar width (same vs different). A 7-item quiz measured the level of perceptual bias. Results showed that the main effects of all three display features. The results revealed that the interaction of bar ordering formats and (1) the scaling styles and (2) the bar thickness patterns on viewers’ perceptual bias were significant. The other effects were not. In addition to extending the theoretical insight into visual deception in the contexts of bar charts, the findings provide practical guidelines for visual makers on bar chart designs that could lead to readers’ perceptual bias, and for visual viewers on how to properly decode the meaning embedded in bar charts.

Function and evolution of Ir52 receptors in mate detection in Drosophila.

Identifying a suitable mating partner is an ancient and critical biological problem. How a fruit fly distinguishes a fly of the same species from flies of innumerable related species remains unclear. We analyze the Ir52 receptors, expressed in taste neurons on the fly legs and encoded by a cluster of genes. We find that the cluster shows dynamic evolution, rapidly expanding and contracting over evolutionary time. We develop a novel invivo expression system and find that Ir52 receptors respond differently to pheromone extracts of different fly species. The receptors are activated by some compounds and inhibited by others, with different receptors showing distinct response profiles. Circuit mapping shows that Ir52 neurons are pre-synaptic to sexually dimorphic neurons that overlap with neurons acting in courtship behavior. Our results support a model in which Ir52 receptors detect information about the species of a potential mating partner.

Comparation of pheromone-binding proteins 1 and 2 of Spodoptera frugiperda in perceiving the three sex pheromone components Z9-14:Ac, Z7-12: Ac and Z11-16: Ac

Pheromone-binding proteins (PBPs) are mainly responsible for binding and transporting hydrophobic pheromone molecules across the aqueous sensilla lymph to the receptor proteins. The preference of each PBP is believed to be different for each pheromone component within a single species. Significantly higher expression level of PBP1 and PBP2 in the male antennae of Spodoptera frugiperda suggesting that SfruPBP1 and SfruPBP2 might play important roles in pheromone perception. However, the preference of these two PBP to the three main pheromone components Z9-14: Ac, Z7-12: Ac and Z11-16: Ac have not been determined. In this study, a fluorescence competitive binding assay revealed that the binding intensities of SfruPBP1 and SfruPBP2 to Z9-14: Ac or Z7-12: Ac was comparable. We then used the CRISPR/Cas9 system to individually or simultaneously knock out PBP1 and PBP2 in S.frugiperda. The result of courtship behavior indicated that SfruPBP1 and SfruPBP2 were indispensable and played equal roles in perceiving the pheromones Z9-14: Ac and Z7-12: Ac for orientation, wing vibration, and hair-pencil display. Compared with Z9-14:Ac and Z7-12: Ac, Z11-16: Ac showed higher or medium binding intensities with SfruPBP1 and SfruPBP2 but played a minor role in inducing the wing vibration behavior. The results of this study are valuable for elucidating the mechanisms involved in sex pheromone perception and may facilitate the development of PBP-targeted pest control techniques.

Males discriminate between substrate-borne cues of conspecific females based on age and mating status in the jumping spider, Habronattus brunneus.

Sexual selection is often studied with a focus on female mate choice, wherein females evaluate male signals to select an optimal mate. However, in some systems, males should also make careful decisions about the females they choose to court, particularly when faced with the risk of precopulatory sexual cannibalism. Here, we explore the idea that male jumping spiders (Habronattus brunneus) may mitigate this risk by responding to female cues probably associated with female aggression and/or receptivity. We tested mature male spiders' ability to discriminate between substrate-borne cues (i.e. silk and excreta) produced by conspecific females of different ages and mating statuses. We found that males spent more time exploring cues produced by mature, non-mated females compared with either immature females or mated females. Heightened interest in cues produced by females that are sexually mature but not yet mated may allow males to reduce cannibalism risk, reduce wasted courtship effort and increase their reproductive success. The use of chemical and/or tactile cues in jumping spider courtship behaviour has been vastly understudied compared with the ways they use vision; this study provides the groundwork for understanding how these sensory modalities interact.

Embryonic development of the world's smallest puffer fish, Carinotetraodon travancoricus - a threatened freshwater fish of the Western Ghats Biodiversity Hotspot.

The Malabar dwarf puffer, Carinotetraodon travancoricus is the smallest known pufferfish (family Tetraodontidae) and one of the smallest freshwater fishes of the Indian subcontinent. Due to their miniature size, wacky behaviour and appearance, they are much preferred in the international aquarium fish trade, although little is known regarding their breeding activity in captivity and their embryonic development. The purpose of this study was to fill these knowledge gaps. Wild-caught Malabar dwarf puffers were acclimatised to conditions, and pairs were introduced to breeding tanks. Adult fishes were fed with live and frozen diets including Artemia nauplii, moina and bloodworm. During spawning seasons, adult fish displayed elaborate courtship behaviour around sunset. Carinotetraodon travancoricus is a batch spawner releasing 1 to 5 eggs per diem. The eggs were spherical, and non-sticky, with a diameter of 1.48 ± 0.1 mm, and hatching took place after 108 to 116 h post-incubation. The newly hatched larvae were 3.5 ± 0.2 mm in length, and weighed 2.9 ± 0.4 mg. The early larvae have substantial yolk and oil globules as an energy reserve. Histological studies on mature females suggested the batch spawning nature of the species and low fecundity. Given its unique reproductive behaviour and characters, in situ protected habitats are required to ensure their continued survival in the wild, apart from encouraging captive breeding to augment the demand in the international aquarium fish trade.

Research on Universal Design of Smart Home Interface Navigation Based on Visual Interaction

The study focuses on analyzing the design characteristics of display and control interface navigation in smart home terminals for users of different ages. By examining the design features of the current typical smart home terminal display and control interface navigation, interface navigation design is the research object. Experimental research is conducted with young adult group (n = 15) and middle-aged to elderly group (n = 15) participants to identify the optimal interface navigation design through specific behavioral indicators, eye movement indicators, and subjective indicators. The results show that different interface layouts impact task completion performance. Middle-aged and older individuals perform better with multi-level highlighting operations. Both age groups prefer interface layouts featuring less-level dual navigation, specifically the left-side type, and multi-level highlighting. These results can improve the versatility of smart home interface design, expand the user base, and provide information for developing smart products.

The Ortholog Receptor Or67d in Drosophila Bipectinata is able to Detect Two Different Pheromones.

Sex pheromones play a crucial role in species recognition and reproductive isolation. Despite being largely species-specific in drosophilids, the mechanisms underlying pheromone detection, production, and their influence on mating behavior remain poorly understood. Here, we compare the chemical profiles of Drosophila bipectinata and D. melanogaster, the mating behaviors in both species, as well as the tuning properties of Or67d receptors, which are expressed by neurons in antennal trichoid sensilla at1. Through single sensillum recordings, we demonstrate that the D. bipectinata Or67d-ortholog exhibits similar sensitivity to cis-vaccenyl acetate (cVA) as compared to D. melanogaster but in addition also responds uniquely to (Z)-11-eicosen-1-yl-acetate (Z11-20:Ac), a compound exclusively produced by D. bipectinata males. Through courtship behavior assays we found that, surprisingly, perfuming the flies with Z11-20:Ac did not reveal any aphrodisiacal or anti-aphrodisiacal effects in mating assays. The behavioral relevance of at1 neuron channels in D. bipectinata compared to D. melanogaster seems to be restricted to its formerly shown function as an aggregation pheromone. Moreover, the non-specific compound cVA affected copulation negatively in D. bipectinata and could potentially act as a premating isolation barrier. As both ligands of Or67d seem to govern different behaviors in D. bipectinata, additional neurons detecting at least one of those compounds might be involved. These results underscore the complexity of chemical signaling in species recognition and raise intriguing questions about the evolutionary implications of pheromone detection pathways in Drosophila species.

Courtship and mating behaviour of the intermediate bow-fingered gecko Cyrtodactylus intermedius in the wild

Courtship and mating behaviour of the intermediate bow-fingered gecko Cyrtodactylus intermedius in the wild

Features of display in account of compensation of losses from written-off stocks during the period of martial state

The paper is devoted to the issues of reflecting in the accounts compensation for losses from written-off stocks during the period of martial law. The relevance of this direction of research is due to the fact that, unfortunately, at enterprises in places where assets are stored, processes of theft, improper storage and deterioration of stocks sometimes occur, as a result of which business entities suffer losses. Therefore, the use of compensation mechanisms by the guilty parties (employees of the enterprise, the aggressor country) and their reflection in accounting lead to a decrease in costs during the write-off of stocks from the enterprise's balance sheet. Every enterprise in Ukraine, during its activity, necessarily records stocks that can be used in production, trade, administration or in other areas of work. Special attention is paid to the places of their storage during the receipt of these assets. We believe that the data of warehouse accounting of stocks should coincide with the data of accounting. But, unfortunately, there are situations in which, after an inventory or military aggression, it is necessary to write off undetected (stolen), damaged or destroyed stocks. In today's conditions, Ukrainian enterprises suffer significant losses as a result of martial law. The use of unmanned aerial vehicles and missiles by Russia, the occupation of a large part of Ukrainian territories, the location of enterprises in war zones or close to them leads to the loss of enterprise assets. We have researched that these losses can occur as a result of direct ammunition hits, fires, and breakdown of equipment used to preserve stocks. Unfortunately, the loss and deterioration of stocks leads to the mismatch of their recognition as assets and the subsequent receipt of economic benefits.

Mating proximity blinds threat perception

Romantic engagement can bias sensory perception. This ‘love blindness’ reflects a common behavioural principle across organisms: favouring pursuit of a coveted reward over potential risks1. In the case of animal courtship, such sensory biases may support reproductive success but can also expose individuals to danger, such as predation2,3. However, how neural networks balance the trade-off between risk and reward is unknown. Here we discover a dopamine-governed filter mechanism in male Drosophila that reduces threat perception as courtship progresses. We show that during early courtship stages, threat-activated visual neurons inhibit central courtship nodes via specific serotonergic neurons. This serotonergic inhibition prompts flies to abort courtship when they see imminent danger. However, as flies advance in the courtship process, the dopaminergic filter system reduces visual threat responses, shifting the balance from survival to mating. By recording neural activity from males as they approach mating, we demonstrate that progress in courtship is registered as dopaminergic activity levels ramping up. This dopamine signalling inhibits the visual threat detection pathway via Dop2R receptors, allowing male flies to focus on courtship when they are close to copulation. Thus, dopamine signalling biases sensory perception based on perceived goal proximity, to prioritize between competing behaviours.

FEATURES OF ACCOUNTING DISPLAY OF FINISHED PRODUCTS AS A COMPONENT OF FOREST RESOURCES

Different disciplines and branches of science (economics, ecology, sociology, etc.) have their own approaches to understanding the concept of «forest resources», which can lead to differences in its interpretation. The article analyzes approaches to understanding the essence of the main categories of forestry, in particular, «forest» and «forest resources». It is noted that the diversity of approaches in defining the concept of «forest resources» is reflected in the variability of the understanding of this definition in the context of ecological, economic, socio-cultural and other aspects. The author's definition of the category «forest resources» as an object of accounting is presented and it is determined that forest resources are part of the country’s natural resources, which include not only forest products, but also its useful properties (for example, recreation and health). The author gives the most common approaches to understanding the essence of the concept of «forest resources», including ecological, economic, socio-cultural, ecosystem and integrated. Depending on the purpose and specifics of the use of the components of forest resources, their totality is divided into several groups: raw resources of wood origin; resources of non-wood origin; resources of animal genesis; multifaceted beneficial functions of the forest. The peculiarities of forest resources as a category of accounting are determined. It has been established that forest resources are recorded as biological assets. The assessment of the main ecological and economic indicators of the functioning of forestry in Ukraine was carried out and the conclusion was made that along with the improvement of the economic indicators of activity in the forestry sector, during the analyzed period, the deterioration of the ecological indicators of forestry activity was observed. The approaches of scientists to the classification of finished products as a component of forest resources were traced and their own classification was proposed according to the economic value and economic benefits from the use of the main and waste products, the rational use of which can provide enterprises with additional economic benefits.

Effects of ttk on development and courtship of male Nilaparvata lugens.

The transcription product of tramtrack (ttk) is an important transcription factor which plays many roles in the regulation of the development, differentiation and chromosome recombination of organisms. Few studies have been reported on the specific functions of ttk in other insects except Drosophila melanogaster. Our aims are to reveal the ttk effects on development and courtship of male rice pest brown planthopper (BPH), Nilaparvata lugens. In this study, we first assayed spatiotemporal expression of ttk in BPH, then treated the fourth nymphs of BPH with dsttk. We found most individuals died before emerging to adults, the adult eclosion rate was only 18.89%. No courtship behavior was found in individuals injected with dsttk. Further research showed that the main frequency of courtship vibration signal (CVS) 431.3 Hz in the individuals injected with dsttk was significantly higher than 223 Hz in the individuals injected with dsGFP, and female adults nearly had no response to the 431.3 Hz CVS. We found that about 81% of the 4-instar nymphs of BPH treated with dsttk died before they emerged as adults, the successfully emerged adults emitted the 431.3 Hz CVS to which female adults did not respond and lost the ability of courtship. This was first finding about the functions of ttk in rice planthopper and illustrated the potential of ttk as target for RNAi to control rice planthopper. © 2024 Society of Chemical Industry.

Mating behavior and three types of mating songs of the sandy beach-dwelling ground cricket Dianemobius csikii (Grylloidea, Trigonidiidae, Nemobiinae)

This study aimed to investigate the mating behavior of the sandy beach-dwelling ground cricket, Dianemobius csikii (Bolívar, 1901) (Orthoptera, Grylloidea, Trigonidiidae, Nemobiinae). The calling songs of males were recorded, and the temporal structures of the songs were analyzed. Subsequently, the courtship song and mating behavior of cricket pairs were observed. The calling song consisted only of monotonous chirps, while the courtship song consisted of similar chirps and ticks consisting of a single pulse. Dianemobius csikii exhibited a relatively longer courtship duration than other cricket species. The female stayed with the male for approximately 25 min while the male emitted the courtship song. The male then changed to the trill song, which is a continuous song, just before copulation. Copulation occurred within 10–40 s of the male emitting the trill song. The courtship behavior differs from that of other well-studied cricket species, such as Gryllinae. The findings of this study provide insight into the mating behaviors of crickets.