Poultry farming has been recognized as one of the most vital sectors for the economy and revenue generation in many countries. For the production of high-quality freshly hatched chicks, effective cleaning and sanitation of the hatchery environment and hatching eggs were crucial components of proper management and hygiene in chicken hatcheries. The current review aimed to assess the efficient ways of mitigating the risk of disease introduction (external biosecurity) and its subsequent dissemination (internal biosecurity) within and between poultry farms and hatcheries. In addition to identifying the variety of risk categories that are applied to various biosecurity industries, this article clarified the equivalent tools, including checklists and/or questionnaires, that can be used to assess biosecurity compliance. The checklist was aimed to evaluate numerous biosecurity protocol categories, including the farm's infrastructure, employees, their education and training, access control mechanisms, cleaning and disinfection procedures, handling of litter and waste, chick control, registrations, and pest management. In conclusion, external biosecurity was critical to preventing infections from entering hatcheries and poultry farms. Questionnaires or checklists were effective instruments for gathering information on biosecurity and evaluating compliance in poultry farms. Keywords: Biosecurity compliance, Checklist, Hazard, Poultry sector